E-Gel ™ Clonewell ™ Ii Gels; Advantages; General Guidelines; Prepare Samples - Thermo Scientific Invitrogen E-Gel G8100 User Manual

™ ™
E-Gel CloneWell II pre-cast agarose gels are designed for use with the E-Gel
and provide a fast, safe, and effective DNA fragment isolation method for DNA cloning workflows.

Advantages

•
Target fragments are collected directly from a recovery well. No gel-purification is required.
•
Contains SYBR
efficiency by avoiding UV transillumination.
General
•
Load gel within 15 minutes of opening the pouch; run the gel immediately after loading.
guidelines
•
Monitor the band of interest carefully as it migrates near the recovery wells. It may be difficult
to see low amounts of DNA in the well.
•
Important! Always wear Safe Imager
lid opened.
•
For guidance on disposal of used gels, see SYBR

Prepare samples

•
Prepare up to 25 μL of sample in 1X Sample Loading Buffer (e.g., use 2.5 μL of 10X Sample
Loading Buffer with 22.5 μL total sample).
10X Sample Loading Buffer is provided with E-Gel
•
Use the indicated amount of DNA per well for single or multiple bands.
•
Divide samples with higher amounts of DNA across multiple wells.
•
Use up to 25 μL total sample volume per well.
•
Dilute high salt samples (certain restriction enzyme and PCR buffers) 2- to 5-fold.
Gel type
E-Gel CloneWell II
™

Prepare gel

1. Remove the gel from the package.
2. Gently remove the combs. Do not allow the
combs to bend or create suction in the
wells during removal.
3. Insert gel cassette into the E-Gel
Snap Electrophoresis Device, starting from
the right edge.
4. Press down on the left side of the cassette
to secure it into the device.
E-G el Power Snap Electroph oresis System User Guide
™
™
Safe DNA stain, eliminating the risk of DNA damage, and improving cloning
Amount of DNA per well
Sample with single band
200-800 ng
E-Gel CloneWell
™
™
Power Snap Electrophoresis Device,
™ Viewing Glasses when viewing the gel with the filter
™
Safe DNA Gel Stain (page 48).
™ Clonewell
Sample with multiple bands
800 ng
™ Power
II gels
™
™ II Agarose Gels.
Total loading
volume
25 μL
25