Table of Contents
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Table of Contents
Summary of Contents for MULTICHANNEL SYSTEMS MEA Series
- Page 1 MEA Amplifier for Inverse Microscopes Manual...
- Page 2 Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH. While every precaution has been taken in the preparation of this document, the publisher and the author assume no responsibility for errors or omissions, or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it.
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Page 3: Table Of Contents
Table of Contents Introduction About this Manual Important Information and Instructions Operator's Obligations Guarantee and Liability Important Safety Advice First Use of the MEA Amplifier Welcome to the MEA Amplifier Setting Up and Connecting the MEA Amplifier First Tests and Tutorial First Functional Tests General Performance / Noise Level Operating the MEA Amplifier... -
Page 5: Introduction
Introduction 1.1 About this Manual This manual comprises all important information about the first installation of the hardware and software, and about the daily work with the instrument. It is assumed that you have already a basic understanding of technical and software terms. No special skills are required to read this manual. -
Page 7: Important Information And Instructions
Important Information and Instructions 2.1 Operator's Obligations The operator is obliged to allow only persons to work on the device, who are familiar with the safety at work and accident prevention regulations and have been instructed how to use the device; ... -
Page 8: Important Safety Advice
MEA Amplifier for Inverse Microscopes Manual 2.3 Important Safety Advice Warning: Make sure to read the following advice prior to install or to use the device and the software. If you do not fulfill all requirements stated below, this may lead to malfunctions or breakage of connected hardware, or even fatal injuries. -
Page 9: First Use Of The Mea Amplifier
First Use of the MEA Amplifier 3.1 Welcome to the MEA Amplifier Raw data from up to 60 electrodes of a microelectrode array (MEA) is amplified by 60 channels of filter amplifiers that are built very small and compact using SMD (Surface Mounted Devices) technology. -
Page 10: Setting Up And Connecting The Mea Amplifier
MEA Amplifier for Inverse Microscopes Manual 3.2 Setting Up and Connecting the MEA Amplifier Warning: Spilled liquid can damage or even completely destroy the electronics of the MEA amplifier. Please be extremely careful when setting up your perfusion system and when starting the perfusion. -
Page 11: First Tests And Tutorial
First Tests and Tutorial 4.1 First Functional Tests Each MEA amplifier has been thoroughly tested at the factory site before delivery. However, you may want to perform some tests yourself before you begin your experiment to exclude any damage that might have occurred during transportation, or to fulfill your own guidelines, for instance. - Page 12 MEA Amplifier for Inverse Microscopes Manual The following screen shot shows a recording from a typical MEA amplifier with a test model probe and a sampling rate of 25 kHz. Double-click a channel in the display to have a closer look.
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Page 13: Operating The Mea Amplifier
Operating the MEA Amplifier 5.1 Signal Amplification and Filters The standard MEA amplifier combines the probe interface with a band pass filter and the signal amplification in one instrument. Different filter settings are used for different applications to enhance the signal-to-noise ratio. The pass band of the filter amplifier should be chosen according to the signal type. -
Page 14: Temperature Control
MEA Amplifier for Inverse Microscopes Manual If you use a MEA-System, the isolated power supply IPS10W that is integrated in the data acquisition computer supplies the power for operating the MEA amplifier. The power is distributed along the MCS high grade cable. Please consider that the amplifier can only operate properly if the supply voltage and current specifications are fulfilled, especially if you use a custom power supply. -
Page 15: Mounting The Mea Probe And Grounding The Bath
Operating 5.3 Mounting the MEA Probe and Grounding the Bath Warning: Do not use too much force. Otherwise you could damage the delicate MEA or contact pins of the amplifier. Put the lid of the amplifier only onto a dry and clean area with the bottom side down. - Page 16 MEA Amplifier for Inverse Microscopes Manual Grounding the bath You can ground the bath with a plain silver wire or with an Ag/AgCl pellet. Only the Ag/AgCl electrode provides a stable intrinsic potential. In practice, the plain silver wire will be good for 90 % of applications.
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Page 17: Stimulation
Operating 5.4 Stimulation Recommended stimulus protocols for MEA electrodes MCS recommends to use biphasic stimulation pulses only with negative phase first. The duration of each phase should be 100 μs and the amplitude between 100 mV and 3000 mV. (See also Potter, S. -
Page 18: Service And Maintenance
MEA Amplifier for Inverse Microscopes Manual 5.5 Service and Maintenance You should clean the contact pins of the amplifier with a soft tissue and pure (100 %) alcohol from time to time, especially if you have problems with the noise level. It may be necessary after a longer time of operation to replace the contact pins. -
Page 19: Troubleshooting
Troubleshooting 6.1 About Troubleshooting The following hints are provided to solve special problems that have been reported by users. Most problems occur seldom and only under specific circumstances. Please check the mentioned possible causes carefully when you have any trouble with the product. In most cases, it is only a minor problem that can be easily avoided or solved. -
Page 20: Unsteady Baseline
MEA Amplifier for Inverse Microscopes Manual Contact pin is defective Possible causes: The contact pins are contaminated. Clean the contact pins carefully with a smooth and clean tissue and pure (100 %) alcohol. The contact pins are damaged. Replace the contact pins carefully. Please see the Spare Parts list under "Ordering Information". Try to handle and clean the contact pins more carefully next time. -
Page 21: Artifacts On All Channels
Troubleshooting 6.4 Artifacts on all Channels You see strange artifacts on all channels. This behavior can be caused by an insufficient supply power. Please see the Technical Specifications section. If the voltage drops beyond a critical level, the amplifier cannot operate properly, resulting in artifacts or in a saturation of the amplifier. Possible causes: The cable connecting the amplifier to the power supply, that is, the MCS high grade cable leading from the amplifier to the data acquisition computer if you have a MEA-System,... -
Page 22: Liquid Spilled Onto Amplifier
MEA Amplifier for Inverse Microscopes Manual 6.5 Liquid Spilled onto Amplifier Warning: Spilled liquid can damage or even completely destroy the electronics of the MEA amplifier. Avoid it by all means. Of course it is best to completely avoid this situation and consider the following instructions as an "emergency measurement"... -
Page 23: Appendix
Appendix 7.1 Technical Specifications 7.1.1 MEA1060-Inv Operating temperature 10 °C to 50 °C Storage temperature 0 °C to 50 °C Dimensions (W x D x H) 165 mm x 165 mm x 20 mm (29 mm with sockets) Weight 800 g Supply voltage (external power ±... -
Page 24: Heating Element
MEA Amplifier for Inverse Microscopes Manual 7.1.2 Heating Element The heating element is mounted onto the bottom side of the base plate of the MEA1060 amplifier. It is used for keeping the temperature in the MEA culture chamber stable during recordings. -
Page 25: Pin Layout
Appendix 7.2 Pin Layout 7.2.1 Pin and MEA Layout The layout of standard MEA electrodes follows the scheme of a standard grid: The first digit is the column number, and the second digit is the row number. For example, electrode 23 is positioned in the third row of the second column. - Page 26 MEA Amplifier for Inverse Microscopes Manual Assignment pin to electrode 68-pin socket MEA electrode 68-pin socket MEA electrode...
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Page 27: Contact Information
Appendix 7.3 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site. User forum The Multi Channel Systems User Forum provides the opportunity for you to exchange your experience or thoughts with other users worldwide. Mailing list If you have subscribed to the Mailing List, you will be automatically informed about new software releases, upcoming events, and other news on the product line. - Page 28 MEA Amplifier for Inverse Microscopes Manual USB-MEA-Systems Product Product Description Number USB-MEA-System for USB- MEA Workstation for recording and analyzing data from upright microscopes MEA240- MEA (microelectrode array) experiments. Data acquisition Up-4- from up to 240 MEA electrodes, 4 analog channels, 1 audio System channel, and 16 digital channels.
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Page 29: Stimulation
Appendix acquisition and filter System amplification. 4 additional analog and 1 digital I/O port. amplifier Complete with data acquisition computer, software package and accessories. USB 2.0 High Speed data transfer with a sampling rate of up to 40 kHz per channel. USB-MEA256-System USB- 256-channel recording system with integrated data... - Page 30 MEA Amplifier for Inverse Microscopes Manual Spare Parts Product Product Description Number Heating element with 8 mm bore HE-Inv-8 For MEA1060-Inv/MEA1060-Inv-BC amplifier. hole Available with bore holes in different sizes, for using smaller or larger microscope Heating element with 12 mm bore HE-Inv-12 objectives.
- Page 31 Appendix 60 electrode channels and accessories MEA recording system for MEA60-Inv-2- Complete with 5 MEAs, data acquisition inverted microscopes with System-E computer with MC_Card, 2 x MEA1060-Inv advanced perfusion, amplifier, MEAS2/1, 2 x TC02, 2 x PH01, 60 electrode channels power supply, and accessories MEA recording system for MEA60-Up-...
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Page 33: Index
Index... -
Page 34: Bandwidth
MC_Card Aliasing layout ..........20 mounting .......... 10 Band Model Test Probe pass band..........8 Mounting Bandwidth MEA Probe ........10 Change MEA Noise troubleshooting........ 14 Configuration Noise Level Connecting testing ..........6 Contact Information Faraday cage layout ..........20 Filters bandwidth ...........8 Signal Frequency...
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