diagenode Bioruptor Pico User Manual
  1. Manuals
  2. Brands
  3. diagenode Manuals
  4. Laboratory Equipment
  5. Bioruptor Pico
  6. User manual

diagenode Bioruptor Pico User Manual

Sonication system
Hide thumbs Also See for Bioruptor Pico:
Table of Contents

Advertisement

Quick Links

Download this manual
Bioruptor
Pico
®
Sonication System
U S E R M A N U A L
V e r s i o n 1 . 1
Bioruptor
Pico
®
Cat. No. B01060001

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Bioruptor Pico and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for diagenode Bioruptor Pico

Summary of Contents for diagenode Bioruptor Pico

  • Page 1 Bioruptor Pico ® Sonication System U S E R M A N U A L V e r s i o n 1 . 1 Bioruptor Pico ® Cat. No. B01060001...
  • Page 2 Diagenode, at its discretion, will repair or replace the product in accordance with the warranty terms and conditions stated herein. In case of repair or replacement of a product under warranty, Diagenode will cover the expenses to return the repaired or replacement product.

  • Page 3: Table Of Contents

    Ordering Information ............Back Cover Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 4: Critical Steps For Maintenance And Efficient Shearing

    Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...

  • Page 5: Introduction

    Diagenode recommends that pregnant women should not be exposed to 20-60 kHz wave lengths for a long period of time. Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 6: Bioruptor ® Pico Technical Specifications

    15 ml tubes – 6 Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...

  • Page 7: Getting To Know Your Bioruptor

    ® Sonication bath Motorized lid Sonication Unit Power Cable (EU) Power Cable (US) Tube holder Water Cooler To learn more, please visit www.diagenode.com Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 8: Single Cycle Valve For Bioruptor

    4°C during the sonication process. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...

  • Page 9: Motorized Lid

    5 µl 10 or 50 µl 0.65 ml 100 µl 100 µl 1.5 ml 200 µl 300 µl 15 ml 500 µl 2 ml Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 10: Equipment Installation

    Water Cooler and the Single Cycle Valve kit. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...

  • Page 11: Installing The Bioruptor

    400 mm or 15,74 inch). 4. Place the Single Cycle Valve on top of the Water cooler as shown in the image. Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...
  • Page 12 8. Plug the power cord into the outlet of the Water cooler. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...
  • Page 13 12. Press Start/Stop key to control the temperature. Note: See Water cooler manual for additional information Now you are ready to start ! Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 14: Controlling The Sonication

    Switch on power switch (back side of sonication unit) Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...
  • Page 15 IMPORTANT NOTE: Machine has to rest for 20 Press STOP minutes before starting the next sonication run. Figure 3. Close Lid Screen Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 16: Tube Holders And Tubes

    C01020031 (Cat. No. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...

  • Page 17: Standard Protocol For Dna Shearing

    Sonication cycle & total sonication time: varies depending on desired DNA size (see table) Note: Recommended protocols are subject to change without notice. Additional protocols are available on demand. Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...
  • Page 18 DNA High Sensitivity chip. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...
  • Page 19 406.9 bp (CV%: 7.96%). All samples were analyzed on Bioanalyzer 2100 DNA High Sensitivity chips. C. Target Size: 300 bp D. Target Size: 400 bp Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 20: Important Comments About Dna Shearing

    Pico sonication system. ® Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...
  • Page 21 3) RNA contamination in genomic DNA preparation should be carefully removed using RNase-DNase free enzymatic digestion since they might generate a biased fragment distribution profile on microfluidics-based platform (e.g. Agilent Bioanalyzer) or alter sonication effiency. Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 22: Small Volume Dna Shearing For Bioruptor

    Note: Recommended protocols are subject to change without notice. Additional protocols are available on demand. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...
  • Page 23 • Adding short centrifugation step(s) during the sonication round (e.g. after half of the cycle numbers) can significantly improve the results. • Protocols for other size ranges are available on request. Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...
  • Page 24 Panel A, B and C: peak electropherogram view. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...

  • Page 25: Important Comments About Dna Shearing

    • Sample concentration: Diagenode recommends using a DNA concentration of 10 ng/ µl. Using larger or smaller concentrations may result in variable peak distribution.
  • Page 26 Agilent Bioanalyzer) or alter sonication effiency. Europe Diagenode sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com...

  • Page 27: Consistent And Highly Reproducible Chromatin Shearing With The Bioruptor

    500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker). (MW corresponds to the 100 bp DNA molecular weight marker) Diagenode Inc. North America / Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na@diagenode.com...

  • Page 28: Pico

    For a complete listing of Diagenode’s international distributors visit: http://www.diagenode.com/pages/distributors.html For rest of the world, please contact Diagenode s.a. © 2010 Diagenode, Inc. All rights reserved. The content of this document cannot be reproduced without prior permission of the authors. Bioruptor and IP-Star are registered trademarks of Diagenode, Inc.

This manual is also suitable for:

B01060001

Table of Contents