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Related Manuals for Thermo Scientific E‑Gel 4466611
Summary of Contents for Thermo Scientific E‑Gel 4466611
- Page 1 ™ E‑Gel Imager Gel Documentation System USER GUIDE For visualization and documentation of gels Catalog Numbers 4466611and 4466612 Publication Number MAN0004868 Revision A.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Life Technologies Corporation | 5781 Van Allen Way | Carlsbad, CA 92008 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
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Page 3: Table Of Contents
Contents ■ CHAPTER 1 Product information ..........6 Product description . - Page 4 Contents Description of edit mode control panel tools ........25 Description of menu based tools .
- Page 5 Contents ■ APPENDIX G Explanation of symbols ......... . . 49 ■...
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Page 6: Chapter 1 Product Information
Product information Product description ™ The E‑Gel Imager Gel Documentation System is a benchtop gel imaging system that supports both fluorescent and colorimetric (visible dye) visualization applications. The system consists of a high-resolution camera for capturing images, and a choice of interchangeable bases to provide transillumination. -
Page 7: Product Contents
Chapter 1 Product information Product contents Product contents Upon receiving the device Examine the unit carefully for any damage incurred during transit. Any damage claims must be filed with the carrier. The warranty does not cover in-transit damage. ™ Types of E‑Gel imager products ™... -
Page 8: Accessory Products
Chapter 1 Product information Product contents (continued) Cat. No. Cat. No. Component 4466611 4466612 ™ E‑Gel Imager Blue Light Base Kit ™ E‑Gel Imager Blue Light Base — ✓ Universal 12 V Power Cord — ✓ Accessory products ™ Accessory products that can be purchased for use with the E‑Gel Imager system are listed in the following section: ™... -
Page 9: Description Of Parts
Chapter 1 Product information Description of parts Description of parts ™ E‑Gel Imager System ™ The E‑Gel Imager is a compact gel imaging tool standing 43.7 cm in height. It ™ consists of the E‑Gel Imager Camera Hood (35.6 cm in height), which fits on top of a Base unit (11.9 cm in height). -
Page 10: E-Gel ™ Imager Camera Hood
Chapter 1 Product information Description of parts E‑Gel ™ Imager Camera Hood The camera hood contains the camera and emission filter. It must be connected to a computer installed with the GelCapture Software in order to operate. Image settings can be adjusted using the software, or manually using the dials on the camera hood. ON/OFF power switch Manual iris dial Manual focus dial... -
Page 11: E-Gel ™ Imager Base Unit
Chapter 1 Product information Description of parts E‑Gel ™ Imager Base Unit ™ Two types of E‑Gel Imager Base are available for visualizing different types of gel stain, and for different applications. There is a bordered wall to prevent liquid spills, and a wall breach for easy removal of gels from the surface of the light base. - Page 12 Chapter 1 Product information Description of parts ™ E‑Gel Imager UV Light Base ™ The E‑Gel Imager UV Light Base is used to visualize ethidium bromide stained gels. ™ This light base can be used to visualize E‑Gel precast agarose gels. An automatic shut-off switch turns off the transilluminator after 10 minutes.
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Page 13: Colored Emission Filters
Chapter 1 Product information Description of parts Colored emission filters ™ Three colored emission filters are available with the E‑Gel Imager System to provide better definition when viewing specific sample types. Place the type of filter appropriate for the type of stain being used into the filter tray when visualizing a gel through the camera. -
Page 14: Chapter 2 Methods
Methods Minimum PC requirements ™ In order to install and operate both the E‑Gel Imager and GelCapture Software, your PC must meet the following minimum requirements: ™ ™ • Intel Core 2 Duo processor, 1.8 GHz. • Minimum 1 GB RAM of memory (2 GB recommended). ™... -
Page 15: Install The E-Gel ™ Imager Camera Hood And Light Base
Chapter 2 Methods Install hardware Install the E‑Gel ™ Imager Camera Hood and Light Base 1. Place the E‑Gel ™ Imager Light Base on a flat surface. 2. Plug the Universal 12 V power cord into the light base power port, and connect the wall plug to an electrical socket. -
Page 16: Using The E-Gel ™ Imager Gel Documentation System
Chapter 2 Methods ‑ Gel Using the E ™ Imager gel documentation system ™ Using the E‑Gel Imager gel documentation system ™ After the software and hardware for the E‑Gel Imager system is set up, the system is ready for use. Read the following chapter for detailed explanations on how to operate the system. - Page 17 Chapter 2 Methods ‑ Gel Using the E ™ Imager gel documentation system 4. Place the E‑Gel ™ ™ Imager Camera Hood on top of the E‑Gel Imager Base. 5. Turn the camera on ( 6. Turn the E‑Gel ™ Imager Base on ( 7.
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Page 18: Launch The Gelcapture Software
Chapter 2 Methods ‑ Gel Using the E ™ Imager gel documentation system Launch the GelCapture Software 1. Launch the GelCapture Software from your PC desktop. 2. Select the type of E‑Gel ™ Imager Light Base being used to provide transillumination from the Home screen (see page 21). -
Page 19: Define Region For Image Capture
Chapter 2 Methods ‑ Gel Using the E ™ Imager gel documentation system Define region for image capture In the Live mode screen you can save, copy, or view histograms in the selected area. 1. Select Area of interest ( ) to define the region of the gel that you wish to image. -
Page 20: Capture And Save Image
Chapter 2 Methods Maintenance Capture and save image 1. Right click in the green AOI frame to display a menu with the AOI options. Note: The options in the menu differ depending on whether you are looking at a real-time image, or working on a previously saved image. 2. -
Page 21: Chapter 3 Gelcapture Software
GelCapture software GelCapture software ™ The GelCapture software application seamlessly integrates with the E‑Gel Imager hardware, giving you complete control of configuration, capturing and saving sample images on your PC for analysis. The friendly GUI lets you perform common actions with a single click of a button or icon. -
Page 22: Live Mode Screen
Chapter 3 GelCapture software Live mode screen Live mode screen Iris and focus settings: Image of recommended settings for the iris and focus. Exposure time: The +/– slide gauge is used to adjust the amount of time the camera sensor collects signal to create an image. -
Page 23: Description Of Live Mode Control Panel Tools
Chapter 3 GelCapture software Description of live mode control panel tools Description of live mode control panel tools Short descriptions of tools that are available in the Live mode screen are listed in the following table. Icon Function Saves an image. Prints an image. -
Page 24: Edit Mode Screen
Chapter 3 GelCapture software Edit mode screen Edit mode screen Capture a new image: Return to Live mode screen. File: The file name of a saved image. Date taken: The date an image was captured. Exposure: The exposure time used to capture the image. Sensitivity: The sensitivity used to capture the image. -
Page 25: Description Of Edit Mode Control Panel Tools
Chapter 3 GelCapture software Description of edit mode control panel tools Description of edit mode control panel tools Short descriptions of tools that are available in the Edit mode screen are listed in the following table. Icon Function Opens the Live mode screen to allow capture of a new image. Saves an image. -
Page 26: Description Of Menu Based Tools
Chapter 3 GelCapture software Description of menu based tools Description of menu based tools The Main Menu bar can be used to access various tools for file management and image adjustment. Tool Function File menu Open tiff file Opens a saved .tif file from your computer. Save image Saves an image (available in Edit Mode only). -
Page 27: About The Histogram
Chapter 3 GelCapture software About the histogram About the histogram The histogram provides the user with a graphical version of the image data to help determine the optimal image capture conditions. When an image is detected, the histogram is displayed at the bottom of the screen. Left cursor Right cursor The y-axis of the histogram displays the number of pixels at a given gray level. -
Page 28: Correct Overexposure
Chapter 3 GelCapture software Correct overexposure Correct overexposure Overexposure occurs when the camera receives too much light. Overexposure can be visualized as white, or bright areas that are difficult to analyze, and that can lead to inaccurate analysis results. 1. Select the Show over exposure checkbox. 2. -
Page 29: Invert An Image
Chapter 3 GelCapture software Invert an image Invert an image Inverting an image is particularly useful for discriminating between close bands. This function inverts the color scale of the image (i.e., light areas appear dark and dark areas appear light, much like for a film negative). 1. -
Page 30: Appendix A Troubleshooting
Troubleshooting Observation Possible cause Recommended action Camera is not detected Camera not turned on, or Turn on the Light Base and Camera Hood prior software launched before to launching the GelCapture software. camera turned on. Cables not properly connected. Ensure the USB 2.0 cable is properly connected to both the Camera Hood and the PC. - Page 31 Appendix A Troubleshooting Apply pseudo colors Observation Possible cause Recommended action Improper illumination intensity Button is receiving incorrect Contact technical support. voltage Low incoming illumination Contact technical support. source voltage Duration of E‑Gel ™ Safe Imager ™ ™ ™ ™ ™...
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Page 32: Appendix B Product Specifications
Product specifications ™ E‑Gel Imager Camera Hood Condition Description Case dimensions 35.6 cm (height) × 30.5 cm (length) × 21 cm (width) Electrical Requirements 100–240 V, 50/60Hz, 0.6A Temperature Ambient ± 5°C to 40°C Adaptor Specifications Use only the UL Listed adaptor supplied with the E‑Gel ™... -
Page 33: Appendix C Software Installation
Software installation Getting started Minimum PC requirements ™ In order to install and operate both the E‑Gel Imager and GelCapture software, your PC must meet the following minimum requirements: ™ ™ • Intel Core 2 Duo processor, 1.8 GHz. • Minimum 1 GB RAM of memory (2 GB recommended). ™... - Page 34 Appendix C Software installation Install GelCapture Software Windows ™ 7 professional 1. Open the CD with internet explorer, right-click the GelCapture.exe installation file, and select Run as administrator. 2. Select Allow, I trust this program. I know where it′s from or I′ve used it before to allow the program to run.
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Page 35: Windows ™ 7 Professional
Appendix C Software installation Installing the camera driver (Windows ™ 7 pro) ™ Installing the camera driver (Windows 7 pro) ™ Installing the camera driver (Windows 7 professional) ™ Before installing the camera driver for Windows 7 Professional, make sure that the ™... - Page 36 Appendix C Software installation Installing the camera driver (Windows ™ 7 pro) 3. From the Devices and Printers settings, open the Device Manger. 4. Connect the USB 2.0 cable from the PC to the E‑Gel ™ Imager, and turn the ™...
- Page 37 Appendix C Software installation Installing the camera driver (Windows ™ 7 pro) 5. Under Other devices, right-click Unknown device and select Update Driver Software. 6. In the Update Driver Software window, select “Browse my computer for driver software”. ‑ Gel ™...
- Page 38 Appendix C Software installation Installing the camera driver (Windows ™ 7 pro) 7. Select “Let me pick from a list of device drivers on my computer”. 8. Choose E‑Gel ™ Imager USB2 and click, Have Disk. ‑ Gel ™ Imager Gel Documentation System User Guide...
- Page 39 Appendix C Software installation Installing the camera driver (Windows ™ 7 pro) 9. In the Install From Disk window, click Browse. 10. Browse to the following location: My computer >> Local Disc (C:) >> Program Files >> EGel Imager >> GelCapture >> drivers >> EGel, and click Open. ‑...
- Page 40 Appendix C Software installation Installing the camera driver (Windows ™ 7 pro) 11. Choose EGel Imager from the E‑Gel ™ Imager folder, and click Open. 12. Click OK. ‑ Gel ™ Imager Gel Documentation System User Guide...
- Page 41 Appendix C Software installation Installing the camera driver (Windows ™ 7 pro) 13. Choose EGel Imager USB2, and click Next. 14. After successfully completing the driver update, the successful update window will be displayed. Click Close. 15. Close the Device Manager and Control Panel windows. The E‑Gel ™...
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Page 42: Appendix D The E-Gel ™ Imager Band Excision Kit
™ The E‑Gel Imager Band Excision ™ Using the E‑Gel Imager Band Excision Kit ™ Use the E‑Gel Imager Band Excision Kit (Cat. No. 4466605) to excise bands from your gel. ™ • When cutting bands from the E‑Gel Imager Blue Light Base, use the ™... - Page 43 Appendix D The E‑Gel ™ Imager Band Excision Kit ‑ Gel ™ Using the E Imager Band Excision Kit Activation switch: A magnetic switch that allows transillumination to be powered on without ™ having the E‑Gel Imager Camera Hood in place. ™...
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Page 44: Excise A Band From The Gel
Appendix D The E‑Gel ™ Imager Band Excision Kit ‑ Gel ™ Using the E Imager Band Excision Kit Excise a band from the gel 1. Turn the E‑Gel ™ ™ Imager camera OFF, then remove the E‑Gel Imager Camera ™... -
Page 45: Appendix E The White Light Converter
The White Light Converter Using the White Light Converter ™ The E‑Gel Imager System is designed to detect gels that are stained with both fluorescent and visible dyes, but requires use of the White Light Converter to detect gels that are stained with visible dyes. White Light Converter E‑Gel ™... - Page 46 Appendix E The White Light Converter Using the White Light Converter 5. Continue to work as you would without the White Light Converter. Note: To work with a new sample, turn the unit OFF, remove the Tower, remove the sample from the Light Base, and clean the Light Base with water and ethanol.
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Page 47: Appendix F Related Products
Related products Accessory products Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that the material is available from fisherscientific.com or another major laboratory supplier. Item Amount Source ™ E‑Gel Imager Gel Documentation System ™ E‑Gel Imager UV Light Base 1 unit Cat. -
Page 48: E-Gel Agarose Gels And Dna Ladders
Appendix F Related products ‑ Gel ™ agarose gels and DNA ladders ™ E‑Gel agarose gels and DNA ladders ™ E‑Gel Agarose Gels are bufferless pre-cast agarose gels with a variety of different agarose percentages and well formats designed for fast, convenient electrophoresis of DNA samples. -
Page 49: Appendix G Explanation Of Symbols
Explanation of symbols Symbol Description The E‑Gel ™ Imager System complies with the Underwriters Laboratories Inc. regulation and the European Community Safety ™ requirements. Operation of the E‑Gel Imager System is subject to the conditions described in this manual. The protection provided by the equipment may be impaired if the equipment is used in a manner not ™... -
Page 50: Appendix H Products No Longer Supported
Products no longer supported ™ ™ The E‑Gel Imager iBase Light Diffuser E‑Gel ™ Imager Adaptor Base ™ ™ ™ The E‑Gel Imager Adaptor Base is used with the E‑Gel Safe Imager ™ ™ Transilluminator to to visualize gels stained with SYBR dyes. -
Page 51: About Multiple Image Acquisition
Appendix H Products no longer supported ‑ Gel The E ™ Imager iBase ™ Light Diffuser About multiple image acquisition Multiple image acquisition is an advanced image capture function to acquire a series of images over time (for example, for bands migrating during gel electrophoresis). -
Page 52: Multiple Image Acquisition Edit Screen
Appendix H Products no longer supported ‑ Gel The E ™ Imager iBase ™ Light Diffuser Multiple image acquisition edit screen Back to thumbnail: Return to Live mode screen. File: The file name of a saved image. Date taken: The date an image was captured. Exposure: The exposure time used to capture the image. -
Page 53: Perform Multiple Image Acquisition
Appendix H Products no longer supported ‑ Gel The E ™ Imager iBase ™ Light Diffuser Perform multiple image acquisition Use Multiple Image Acquisition to capture images of band migration during gel electrophoresis. 1. Click the Multiple Image Acquisition button. 2. - Page 54 Appendix H Products no longer supported ‑ Gel The E ™ Imager iBase ™ Light Diffuser 9. Click Stop to cancel image capture. 10. When the acquisition process is completed, or acquisition is aborted, the Capture a new image button appears on the screen. ‑...
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Page 55: Additional Multiple Image Acquisition Functions
Appendix H Products no longer supported ‑ Gel The E ™ Imager iBase ™ Light Diffuser Additional multiple image acquisition functions The Background function for Multi Images Acquisition process creates images that are made of a combination of previous and new information. The background for a second image is based on information from both the first and second images, although the second image was captured with the same exposure time as the first image in the series. -
Page 56: Using The E-Gel ™ Imager Light Base With E-Gel ™ Ibase ™ Device
Appendix H Products no longer supported ‑ Gel ‑ Gel Using the E ™ imager light base with E ™ iBase ™ device ™ ™ ™ Using the E‑Gel imager light base with E‑Gel iBase device Hardware configuration ™ ™ The E‑Gel Imager Blue Light Base or E‑Gel Imager UV Light Base can be used to... -
Page 57: Using The E-Gel ™ Imager Adaptor Base
Appendix H Products no longer supported ‑ Gel Using the E ™ Imager Adaptor Base ™ ™ ™ Connect the E‑Gel iBase Device with the E‑Gel Imager Light Base 1. Place the E‑Gel ™ ™ ™ iBase Device on E‑Gel Imager Light Base. - Page 58 Appendix H Products no longer supported ‑ Gel Using the E ™ Imager Adaptor Base ™ Install the E‑Gel Imager Camera Hood and Adaptor Base 1. Place the E‑Gel ™ Imager Adaptor Base on a flat surface. 2. Plug the 48-V power supply into the 48 V power port, and connect the wall plug to an electrical socket.
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Page 59: Hardware Configuration
Appendix H Products no longer supported ‑ Gel Using the E ™ Imager Adaptor Base Hardware configuration ™ ™ ™ The E‑Gel Imager Adaptor Base is used with the E‑Gel Safe Imager ™ Transilluminator to to visualize gels stained with SYBR dyes. - Page 60 Appendix H Products no longer supported ‑ Gel Using the E ™ Imager Adaptor Base ™ ™ ™ Connect the E‑Gel iBase /Safe Imager Transilluminator with the Adaptor Base 1. Connect the DC connector cable to the E‑Gel ™ ™ ™...
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Page 61: The E-Gel ™ Imager Ibase ™ Light Diffuser
Appendix H Products no longer supported ‑ Gel The E ™ Imager iBase ™ Light Diffuser ™ ™ The E‑Gel Imager iBase Light Diffuser ™ ™ Using the E‑Gel Imager iBase Light Diffuser ™ ™ The E‑Gel Imager iBase Light Diffuser (Cat. No. 4473062) is designed to improve ™... - Page 62 Appendix H Products no longer supported ‑ Gel The E ™ Imager iBase ™ Light Diffuser 3. Remove the protective film from the surface of the diffuser. 4. Place the diffuser on the E‑Gel ™ ™ iBase Device so that the velcro strips on the ™...
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Page 63: Appendix I Safety
Safety WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document. -
Page 64: Chemical Safety
Appendix I Safety Chemical safety • Do not open the unit. Due to the danger of exposure to high voltage, only trained service technicians should open the unit. • Do not place the system near any motorized, vibrating, or magnetic equipment. •... -
Page 65: Biological Hazard Safety
Appendix I Safety Biological hazard safety Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. Conduct all work in properly equipped facilities with the appropriate safety equipment (for example, physical containment devices). -
Page 66: Documentation And Support
Documentation and support Customer and technical support Visit thermofisher.com/support for the latest service and support information. • Worldwide contact telephone numbers • Product support information – Product FAQs – Software, patches, and updates – Training for many applications and instruments •... - Page 68 E-Gel Imager_UG_MAN0004868-v1-GUID-93F6BB57-826D-47E1-86B2-8581C36DE233-2018/09/13 13:29:35 en 15:24:59.097-04:00 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 16 July 2020...
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