Conditioning - Metrohm 940 Professional IC Vario TWO Manual

3.21 Conditioning

3.21 Conditioning
￭￭￭￭￭￭￭￭
42
6 Inserting the separation column
Insert the separation column with the chip into the column holder
￭
until you hear it snap in place.
The separation column is now detected by MagIC Net.
In the following cases, the system must be conditioned with eluent until a
stable baseline has been reached:
After installation
￭
After each time the instrument is switched on
￭
After each eluent change
￭
NOTE
The conditioning time can lengthen considerably if the composition of
the eluent is modified.
Conditioning the system
1 Preparing the software
CAUTION
Ensure that the configured flow rate is not higher than the flow
rate permitted for the corresponding column (refer to the column
leaflet and chip data record).
Start the MagIC Net computer program.
￭
Open the Equilibration tab in MagIC Net: Work-
￭
place ▶ Run ▶ Equilibration.
Select (or create) a suitable method.
￭
Also see: MagIC Net Tutorial and online help.
2 Preparing the instrument
Ensure that the column is inserted correctly in accordance with
￭
the flow direction marked on the sticker (arrow has to point in the
direction of flow).
Ensure that the eluent aspiration tubing is immersed in the eluent
￭
and that there is enough eluent in the eluent bottle.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
940 Professional IC Vario TWO (2.940.2100)