STEMCELL STEMvision Technical Manual page 33

Figure 13. Human STEMvision™ Colony Marker Application upon Startup

Menu Bar: Located along the top of the application window.

View Window: Located below the Menu Bar. The loaded image will be displayed here along with the
analysis results (if loaded) as colored circles surrounding each individual colony. The colony ID
number is shown near the top left of the colored circle surrounding each colony.

Colony List: Located on the right side of the application window. Gives a tabular summary of each
colony, indicating its ID number so that it can be identified in the image. For human assays it shows
the summary of CFU type (i.e. CFU-E, BFU-E, CFU-G/M/GM or CFU-GEMM) and for mouse assays
the summary of CFU size class (i.e. size class 1, 2, etc.) that has been scored by STEMvision™.

Colony Count Summary: Located on the bottom right corner of the application window. Summarizes
the numbers of different types of colonies in the analyzed culture.
1. Click on "File" in the Menu Bar and select "Open Image". Browse through the folders and select the image
you want to view. This will open up the acquired image only (i.e. the different colony types will not be
shown). Human 14-Day dark-field images may be opened separately, if desired. If the selected file is a
bright-field image and there is a corresponding dark-field image in the folder, the image shown in the
preview window will be a color composite of both the bright-field and dark-field images.
Note: Images may be dragged and dropped directly from a file manager window into the preview window.
2. Click on "File" in the Menu Bar and select "Open Data". Browse and select the data (txt) file generated
during the image analysis (i.e. ImageName_AssayType_Result.txt).
Note: Data may be dragged and dropped directly from a file manager window into the "Colony List" field.
Colonies will be marked on the image as shown in Figure 14 (human) or Figure 15 (mouse).
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