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4Deep HoloSea S7 User Manual

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Model S7
HoloSea Microscope User Guide

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Summary of Contents for 4Deep HoloSea S7

  • Page 1 Model S7 HoloSea Microscope User Guide...
  • Page 2 Notices Safety and Information Notices Copyright © 4Deep inwater imaging 2018 An “Important” signifies helpful information in No part of this document may be reproduced using the software/hardware. It identifies an in any format without prior agreement or writ- important piece of information to guide the ten consent from 4 Deep inwater imaging.
  • Page 3 HoloSea Microscope...

  • Page 4: Table Of Contents

    CONTENTS Contents 1 Overview 1.1 Purpose ......... . 1.2 Benefits .
  • Page 5 LIST OF FIGURES List of Figures The preassembled HoloSea Microscope (vertical view in left panel and horizontal view in right panel)........10m Cable .

  • Page 6: Overview

    4Deep software. This user guide is strictly related to the installation, use, care of and functionality of the HoloSea. For a detailed description of the setup and operation of the 4Deep software, please refer to the respective user guides. The HoloSea is a submersible holographic microscope; for the benchtop microscope, see the Desktop Microscope.

  • Page 7: Introduction

    • Simple: no need to focus. 2 Introduction 2.1 Safety Information The user should read this user guide and any other additional information supplied by 4Deep before operating the instrument. This microscope contains a class IIIB Laser. A 405 nm wavelength is emitted from the laser housing itself.

  • Page 8: Components Of The System

    – Windows 10 Pro is preferred over other versions (such as Home) 2.3 Components of the System Be careful when unpacking the contents of the package. As the 4Deep microscope images at the micron level, foreign objects (dust, dirt, fingerprints, smudges, perspiration, saliva etc.) on the camera window and/or the point source can greatly affect the quality of the captured...

  • Page 9: The Preassembled Holosea Microscope (Vertical View In Left Panel And Horizontal View In Right Panel)

    2 INTRODUCTION HoloSea S7 Figure 1: The preassembled HoloSea Microscope (vertical view in left panel and horizontal view in right panel). • Long (10m) cable with 13 pin connector to power laser, camera, and transmit data from the camera to the host computer.

  • Page 10: Power Supply (12 Vdc)

    HoloSea S7 2 INTRODUCTION Figure 3: Power Supply (12 VDC) • Carrying case Figure 4: Carrying case, with the microscope inside Please ensure all components listed above were shipped to you. If not, please contact a 4Deep representative immediately.

  • Page 11: Recommended Extras (Not Included)

    The following is a list of recommended extra components, suggested by 4Deep, to optimize the use of your 4Deep HoloSea. Note that none of these are necessary for the use of the HoloSea and the HoloSea is fully functional without.

  • Page 12: Chamber 1

    HoloSea S7 2 INTRODUCTION Figure 5: HoloSea components 2.5.1 Chamber 1 This Chamber contains a digital camera. The camera’s image sensor is protected by a sapphire glass window. The sapphire window also has a copper ring around it, to reduce the effect of biofouling on the instrument (shown in Figure 6).

  • Page 13: Chamber 2

    2 INTRODUCTION HoloSea S7 Figure 6: Protective sapphire camera window and copper ring. Be careful when handling the microscope. At the micron level, smudges on the windows can greatly affect the quality of the captured holograms. Take care not to smudge the windows.

  • Page 14: Crown

    Figure 8: The two purge valves on the bottom of the microscope. The purge valves do not need to be used to ensure the microscope operates correctly. If a 4Deep representative deems it necessary for the purge valves to be opened, it will be completed at the 4Deep office, NOT BY THE USER.

  • Page 15: Installation And Use

    3 INSTALLATION AND USE HoloSea S7 3 Installation and Use 3.1 Connect the HoloSea cable Firstly, ensure the connector pins (male and female) are clean, and free of any dirt/dust before connecting. The connector pin manufacturer suggests using Molykote 44 Medium grease before every mat- ing (Figure 9).

  • Page 16: Screw On Locking Sleeve

    HoloSea S7 3 INSTALLATION AND USE Figure 10: HoloSea bulkhead with 13 pin connector. The male connector is in the left panel, and is attached to the underwater cable. The female is shown in the right panel and is attached to the microscope.

  • Page 17: Connecting To The Computer

    3 INSTALLATION AND USE HoloSea S7 Figure 12: Use of female to female Ethernet connector. In the right panel, the black cord is the rigid Ethernet cable from the underwater cable, and the blue cable is the flexible Ethernet cable to the computer.

  • Page 18: Testing And Deployment

    Figure 16: Dimensions of the Submersible, reported in inches 3.5 Collecting Holograms To collect holograms, it is important to setup both the microscope and Octopus (4Deep’s software) properly. The steps below detail how to setup and use Octopus in conjunction with a 4Deep...

  • Page 19: Acquisition Setup For Octopus And The Microscope

    3 INSTALLATION AND USE HoloSea S7 microscope. Note that you should be using both this user guide, as well as the user guide for Octopus for complete setup. You will also notice that much of the information listed below is also listed in the Octopus User Guide.

  • Page 20: Select Instrument Preset Dialog

    HoloSea S7 3 INSTALLATION AND USE – Select the Preset Option for the HoloSea: Settings -> Reconstruction Options-> Preset. Figure 18: Select instrument preset dialog Go to Octopus User Guide for further details on capturing holograms (Section 4 in the Octopus User Guide).

  • Page 21: Tips For Optimal Hologram Acquisition

    The distance between the camera sensor and the point source - Source to Screen Distance (SSD) in the HoloSea is fixed, based on the configuration of the microscope. The standard configuration provides 54 mm SSD, and offers 15 mm of imageable space. Contact 4Deep for the development of custom configurations for special applications.
  • Page 22 HoloSea S7 4 CARE AND MAINTENANCE 1. You microscope does NOT have to be cleaned for the first deployment. Just ensure the sapphire windows are free of dust/dirt by connecting to a computer (see Section 3) 2. Before EVERY deployment the HoloSea connectors should be mated with grease (see Sub- section 3.1)

  • Page 23: Troubleshooting

    5 TROUBLESHOOTING HoloSea S7 5 Troubleshooting Issue Solution Camera not connecting Check that the cables are properly attached, and power is on If there are more than one Ethernet ports on the computer, try a different one If you are using a laptop...

  • Page 24: Software

    Please refer to the Swordfish, Stingray and Octopus brochures and manuals available at www.4- deep.com for the additional details on software installation and use. Custom software solutions can be developed by 4Deep based on customer specifications. Contact us for details.

  • Page 25: Specifications

    7 SPECIFICATIONS HoloSea S7 7 Specifications Optical Resolution 1.5 microns Laser pulse rate 0.5 µs Tow rate 8 knots Point source wavelength 405 nm Power < 5 Watts Power requirement 120/240 VAC Communication 13 pin SeaConn Ethernet connector Data transfer...

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