Kent Scientific SomnoSuite User Manual
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Kent Scientific SomnoSuite User Manual

Low flow anesthesia system for mice and rats
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SomnoSuite
®
Low Flow Anesthesia System
for Mice and Rats
User's Guide

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Summary of Contents for Kent Scientific SomnoSuite

  • Page 1 SomnoSuite ® Low Flow Anesthesia System for Mice and Rats User’s Guide...
  • Page 2 SomnoSuite ® Anesthesia System User’s Guide Technical Help: 860-626-1172 ©2019 Kent Scientific Corporation, Documentation Version 1.02 for On-Unit Help Version 4.02 and Firmware Revision 2.0.1 - August 2019...

  • Page 3: Table Of Contents

    TABLE OF CONTENTS Introduction System Components ..............1 Navigating SomnoSuite .............. 2 SomnoSuite® Small Animal Anesthesia..............3 Set Up Hardware ................3 Initial Setup ................... 8 Procedure for Use ............... 8 Troubleshooting ................12 System Customization Custom Run Screen ..............13 Alarms ....................15 RoVent®...
  • Page 4 CapnoScan® Set Up Hardware .................31 Initial Setup .................32 Procedure for Use ..............33 Troubleshooting .................34 CapnoScan Sensor Maintenance ..........34 RightTemp® Set Up Hardware ................35 Initial Setup .................36 Procedure for Use ..............37 Troubleshooting .................38 RightTemp Pad and Sensor Maintenance ......38 Data Management PC Connections ................39 History - Internal Storage ............

  • Page 5: Introduction

    Introduction The SomnoSuite is not designed, intended, or authorized for use in human applications. System Components Front Panel Back Panel...

  • Page 6: Navigating Somnosuite

    Navigating SomnoSuite® Module Screens The SomnoSuite Run screen has a designated screen for each installed module. Touch the tabs along the top of the screen to view the parameters and important settings for each module. Touch the tab again to open the module’s extended Setup Menu.

  • Page 7: Somnosuite Small Animal Anesthesia

    Connect the Compressed Gas tubing directly to the Compressed Gas port. • Set gas pressure to 10-15 PSI . Tip: If your gas source does not regulate to this range, connect a Kent Scientific Pressure Reducer between the gas source and the SomnoSuite.
  • Page 8 Tubing Configurations: Connecting an Induction Chamber and a Nose Cone Depending on your specific module configuration, your SomnoSuite includes a set of 2-Accessory Connectors or 3-Accessory Connectors. These are used to divide the SomnoSuite’s airflow to multiple stations or animals.
  • Page 9 SomnoSuite ports and accessories are color-coded for convenience. White Ports and Connectors Inlet and Inspiratory Line Black Ports and Connectors Exhaust and Expiratory Line Blue Clips Branch 1 White Clips Branch 2 Yellow Clips* Branch 3 *3-Accessory Connectors only. Setup Examples...
  • Page 10 SomnoSuite with 3-Accessory Connector for Chamber and two Nose Cones SomnoSuite with 3-Accessory Connector for Chamber, Intubation Stand, and RoVent...
  • Page 11 SomnoSuite with External Ventilator Please see recommended flow rate chart on page 47 SomnoSuite with Stereotaxic Mask...

  • Page 12: Initial Setup

    The SomnoSuite Run Screen Initial Set-Up The first time the SomnoSuite is used, or any time the accessories have changed, be sure to confirm the Anesthesia Delivery settings. Procedure for Use 1) From the Anest Run Screen, touch Setup > Anesthesia to enter syringe settings:...
  • Page 13 2) Touch Setup > Air Source to choose the carrier gas source. Room Air: Utilizes the SomnoSuite’s internal pump to draw in surrounding air Compressed gas: Utilizes a 10-15 PSI gas source connected to the Compress Gas Port. 3) Install the filled syringe into the syringe block holder, tightening the Anesthetic Delivery tube to the syringe tip.
  • Page 14 5) From the Anest Run Screen, touch Induction. Tip: The SomnoSuite will deliver at a preset flow rate of 500mL/min. Try manually increasing the flow rate to reduce induction time. Press then turn Dial A to adjust the gas flow.
  • Page 15 13) At the end of the procedure, reduce the anesthetic concentration to 0%. 14) Touch Nose Cone again to stop air flow. Allow the animal to recover. Syringe Empty Position Calibration The SomnoSuite syringe empty points are pre-calibrated. If you need to change the fac- tory syringe calibrations: 1. Touch...

  • Page 16: Troubleshooting

    Try using a different syringe to see if the problem improves. Anesthetic depth too • SomnoSuite was • Take care not to overprime the SomnoSuite. deep incorrectly primed Liquid isoflurane should never be injected into the vaporizer block during priming. •...

  • Page 17: System Customization

    System Customization Custom Run Screen SomnoSuite allows you to configure your own Run Screen, allowing a combination of parameters from any installed modules to appear on a single screen. When using a Custom Run Screen, all settings need to be entered from the Menu or configured with Dial A and B.
  • Page 18 To use a dial command: At the Run Screen, quickly turn the Dial assigned to the setting whose value you want to change. SomnoSuite beeps and the associated text becomes blue. Turn the Dial to increase or decrease the value for the assigned setting.

  • Page 19: Alarms

    Touch Data Mgmt, then Alarms. Silence all Alarms If you do not wish for the SomnoSuite to make an audible tone when an alarm is triggered, you can silence them. Turn Dial B to choose “Silence all Alarms” or “Allow Sound”. Touch Back when finished.
  • Page 20 Fixed Alarms The SomnoSuite includes a number of fixed alarms that alert you to scenarios that require attention. The number of Fixed Alarms are dependent on the number of modules installed. You may view and configure any of the Fixed Alarms by selecting it, then pressing Dial A to enter the Alarm Menu.

  • Page 21: Rovent

    Set Up Hardware Please see the SomnoSuite instructions on page 3. Scavenging: Connect a charcoal canister to the Exhaust port on the back of the SomnoSuite. Air Source: If you are using a Compressed Gas Source: 1. Connect the Compressed Gas tubing directly to the Compressed Gas port on the back of the SomnoSuite.
  • Page 22 SomnoSuite with 2-Accessory connector for Chamber and Intubation Connecting Ventilator Tubing Only to an Endotracheal Tube: Connect the intubation connector tubing directly to the INSP and EXP ports on the front of the SomnoSuite.

  • Page 23: Initial Setup

    Measure Deadspace The Deadspace calibration will measure the volume of tubing between the SomnoSuite RoVent module and the animal. It is important that the Deadspace is recalibrated every time the tubing configuration is changed to maintain accurate tidal volume delivery.

  • Page 24: Procedure For Use

    4) Press Dial B. When asked to confirm measurement, press Dial B again. Procedure for Use RoVent With SomnoSuite Anesthetic Delivery: 1. Place the animal in the induction chamber. 2. From the Anest Run Screen, enter all Setup settings. (See page 8.) 3.
  • Page 25 Tip: The SomnoSuite will deliver at a preset flow rate of 500mL/min. Try manually increasing the flow rate to reduce induction time. From the Anest Run Screen, press and turn Dial A to adjust the air flow. 5. Turn the Anesthesia Concentration Knob to the desired concentration for induction.
  • Page 26 4. Enter the Anest Run Screen, and turn the Anesthesia Concentration Knob to the desired concentration. SomnoSuite calculates the respiration rate and tidal volume using the animal’s body weight. To change these settings, turn Dial A or Dial B. Touch...
  • Page 27 Configure Settings Configure all ventilator settings from the Vent Run Screen. Pressure Priority : RoVent ventilates to a specified inspiratory pressure. The default target pressure is 15cmH2O independent of body weight setting. To change the target pressure, touch Set Target Pressure.

  • Page 28: Raw Override Mode

    1. From the Vent Run Screen, touch Setup, then More Ventilator. 2. Touch Triggers Menu. 3. Turn Dial B to choose an action. Some actions will require additional settings. Please contact Kent Scientific if you need assistance in enabling a trigger function. Raw Override Mode: For setups where the RoVent’s safety parameters are not desired, the RoVent can be...

  • Page 29: Troubleshooting

    RoVent Troubleshooting Alarm Messages Problem Possible Cause Solutions Tube open to The highest measured Check the tubing between the INS and EXP atmosphere pressure in a breath cycle is ports for leaks or loose connections. under 25% of the target and the tidal volume is over target.
  • Page 30 More Ventilator, and Calib&Tests. Purge Clears any accumulations from the valves using high pressure air flow. Resulting Meaning Possible Action Message Success No leaks found. None is needed. Low baffle There is an internal Contact Kent Scientific. pressure RoVent problem.
  • Page 31 Kent Scientific. connection or a crack or hole in the tubing. Low baffle There is an internal Retest making sure that the tubing is not flexed or pressure or RoVent problem. moved. If the error recurs, contact Kent Scientific. Baffle leaks...

  • Page 32: Mousestat® Set Up Hardware

    Heart Rate Monitor Set Up Hardware Sensor: 1. Connect the interface cable to the MouseSTAT port on the back of the SomnoSuite. 2. Connect the sensor to the interface cable and ensure that the connection is secure. The MouseSTAT Run Screen:...

  • Page 33: Procedure For Use

    Procedure for Use MouseSTAT Settings 1) Anesthetize the animal and place on the RightTemp warming pad. Tip: Kent Scientific recommends the use of inhalant anesthetics. Injectable anesthetic agents have a greater effect on circulation, making pulse oximetry challenging. Always warm the animal to support circulation in the animal’s extremities.

  • Page 34: Troubleshooting

    MouseSTAT Troubleshooting Problem Possible Cause Solutions There is no red light Sensor not fully connected • Ensure that all connections are tight, especially at the back of the controller. blinking light in the sensor. Damaged wire in sensor or • Replace the damaged cable.

  • Page 35: Set Up Hardware

    The CO2 Run Screen Set Up Hardware Sensor: 1. Connect: 1) the interface cable to the CapnoScan port on the back of the SomnoSuite. 2) the side-stream sensor to the interface cable, ensuring that the connection is secure. 2. Insert: 1) the sampling assembly into the back slot of the side stream unit.

  • Page 36: Initial Setup

    SomnoSuite CapnoScan Setup Initial Setup CapnoScan Calibration: 1. From the Co2 Run Screen, touch Setup. 2. Touch More CO2, then Calib CO2 Sensor. Press B to zero the sensor.

  • Page 37: Procedure For Use

    Percent NO2: Set the concentration of NO2 in the balance gas. Note: Whenever these settings are changed, disconnect the CapnoScan from the SomnoSuite, then reconnect it. This will reinitialize the sensor so that the new settings take effect. 3) Connect the animal to the ventilator.

  • Page 38: Troubleshooting

    CapnoScan not running or • Ensure that all connections are tight, readings. not connected especially at the back of the SomnoSuite. • Ensure that the CapnoScan is running. Sampling tube dirty or • Ensure that the sampling tube is not wet, damaged soiled or damaged.

  • Page 39: Set Up Hardware

    Homeothermic Warming Set Up Hardware 1. Connect the warming pad to the Pad Power port on the front of the SomnoSuite. 2. Choose one probe as the body temperature sensor, and the other as the pad temperature sensor. • The probes are fully interchangeable.

  • Page 40: Initial Setup

    5. Secure the pad temperature sensor in the center of the warming pad using tape, etc. If using the included disposable pad covers, make sure the sensor is positioned prior to covering the pad. 6. We recommend that you secure the Pad Temperature Sensor to the warming pad directly beneath the animal.

  • Page 41: Procedure For Use

    Homeothermic Regulation: Homeothermic regulation allows you to set and control the body temperature of the animal. The mode requires the use of the body and pad temperature sensors. To use Unregulated Warming, connect both pad and body sensors. 1. Use the – and + icons to set the target body temperature of the animal. 2.

  • Page 42: Troubleshooting

    • Reconnect the temperature sensors. connected. Sensor failure • Contact Kent Scientific for assistance. RightTemp Pad and Sensor Maintenance • Use the disposable warming pad covers to prevent soiling the warming pad. • Wipe the warming pad clean with a damp cloth if needed. Never saturate the warming pad.

  • Page 43: Data Management

    Data Management For PC Connections Kent Scientific devices can collect and monitor data using a serial over-USB connection. The data can be uploaded to a variety of programs, including ADI’s data acquisition software, LabChart®. If you do not have LabChart, please contact Kent Scientific for recommended alternatives.

  • Page 44: History - Internal Storage

    2. From any Run Screen, Touch: 1) Menu, then Data Management, then Upload 3. Choose: 1) The Parameters to upload 2) The timing 4. Then touch Enable. The “Always enabled” setting saves data as long as the controller is on. The “Enabled while running” setting is available only if your system contains a RoVent ventilator, and saves data only when the ventilator is running.
  • Page 45 To view saved History: 1. Touch Menu, Config, Data Management, then History. Touch View. 2. From the Menu, touch Return. The SomnoSuite will give you options for your return screen. Select Hist to view history recordings. To export History to a PC: 1.

  • Page 46: Analog

    The Analog Output feature sends an analog signal corresponding to a single Parameter to external equipment via a remote cable. Please contact Kent Scientific for a remote cable if you would like to use this feature. To configure Analog Output 1.

  • Page 47: General Information

    This product is not designed, intended or authorized for use in human applications. Product Warranty The SomnoSuite has a one (1) year warranty including all parts and labor charges. This warranty does not cover damage by any cause including, but not limited to, any mal-...

  • Page 48: Satisfaction Guarantee

    Prior to shipment, please clean and decontaminate the product of any chemical, biolog- ical, or isotopic contamination. Please include a completed Product Return Form with the shipment. This form can be obtained by contacting Kent Scientific Customer Service at 888-572-8887 or 860-626-1172.
  • Page 49 RoVent Volume controlled, pressure controlled Control Modes I:E Ratio 1:1 to 1:5 PEEP Built-in Automatic or Manual Respiratory Assist Sigh Pressure 2cmH2O to 30cmH2O Sigh Breath Automatic or Manual Respiratory Rate Range 20bpm to 400bpm Tidal Volume Range 0.01 to 12.0mL Weight Range 3g to 1,250g MouseSTAT...
  • Page 50 Conditions Operating: 0°C to 45°C, 10% to 90% RH, non-condensing Storage: -40°C to 70°C, <90% RH, non-condensing SomnoSuite, RoVent, CapnoScan, MouseSTAT and RightTemp are registered trademarks of Kent Scientific Corporation. Windows is a registered trademark of Microsoft Corporation. LabChart is a registered trademark of ADInstruments Pty Ltd.

  • Page 51: Recommended Flow Rates

    Refer to your User’s Manual for complete instructions. The SomnoSuite uses the Body Weight setting to adjust the flow rate for a Low Profile Face Mask. If another mask style is used, adjust the flow rates manually by navigating to More Vaporizer >...

  • Page 52: Index

    Index airflow ....4, 10, 12, 20-22 capnoscan setup ......32 export ......40, 41, 45 air source ......3, 12, 17 charcoal ........3, 17 external ventilator ....7, 10 alarms ........15, 16, 45 chart ..........8, 13 fixed alarms ........16 always enabled ......40 clamps ..........
  • Page 53 kent device ......39, 41 parameters ......2, 13, 15, righttemp ..29, 36, 38, 45, 46 33, 39, 40 leak test ........25-27 room air......... 3, 9, 12 param locations ......13 low baffle pressure .... 26, 27 rr range ..........33 parity ..........39 maintenance ..
  • Page 54 triggers menu ......24 tubing configuration ..4, 17, 19 tv ............26 type anest ........8 unregulated ........37 upload........39, 40 usb connection......39 vaporizer......9, 12, 47 vaporizer block .......9, 12 ventilator ..... 7, 10, 17-26, 31, 33, 40 view ....2, 9, 16, 29, 40, 41 volleft ..........12 volume priority ......23 warming ......29, 35-38...
  • Page 55 1116 Litchfield Street Torrington, Connecticut 06790 Local: 860-626-1172 Toll-Free: 888-572-8887...

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