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Planer Kryo 1060 Series Operator's Manual

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Kryo 1060
(180 & 380)
OPERATOR'S MANUAL

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  • Page 1 Kryo 1060 (180 & 380) OPERATOR'S MANUAL...
  • Page 2 Issue 06 - Aug 2013 Microsoft is a registered trademark, and Windows is a trademark of Microsoft Corporation in the United States of America and other countries. Planer plc reserve the right to alter products and their specifications without notice.

  • Page 3: Notices

    Hard copies and backup copies should be routinely kept. All equipment supplied by Planer plc. is subject to a Warranty Agreement and Limited Liability. In addition, software products are not sold but are licensed for use and are subject to a Licence Agreement.

  • Page 4: Symbols Used In This Manual

    Symbols Used in this Manual Failure to follow these instructions could result in personal injury. Failure to follow these instructions could result in damage to equipment or samples. This indicates first-aid advice. Symbols Used on the Equipment Caution: Refer to this manual and accompanying documentation Mains supply ON Mains supply OFF...

  • Page 5: Safety Precautions

    SAFETY PRECAUTIONS IT IS IMPORTANT TO READ THIS SAFETY NOTICE PRIOR TO OPERATING ANY EQUIPMENT First Aid IF IN DOUBT, SEEK IMMEDIATE MEDICAL ATTENTION. If any quantity of liquid nitrogen comes into contact with the skin or eyes, immediately flood that area of the body with large quantities of unheated water, apply cold compresses and seek medical attention.
  • Page 6 (such as a pump) into the liquid. P Use tongs or wear cryogloves when handling cold or hot objects. Cryogloves are available as an accessory from Planer...
  • Page 7 P The Sample PRT socket inside the chamber' is only to be used with a Planer Sample PRT. P When heating under manual control, check the chamber temperature at intervals of 30 seconds or less to avoid overheating.

  • Page 8: Emc Precautions

    EMC PRECAUTIONS The following precautions must be taken to ensure that the equipment is not damaged by electrostatic discharge (ESD), and that its immunity to radio frequency interference is not compromised. | Ensure that the Controller is not running a program and that the mains power is disconnected before connecting or disconnecting any cable.

  • Page 9: Table Of Contents

    Contents Notices..........i Symbols used in this Manual.
  • Page 10 viii Shutting Down the System......3-3 Power Failures........3-3 Dealing with a System Failure During a Run.

  • Page 11: An Introduction To The Kryo1060

    An Introduction to the Kryo1060 C H A P T E R 1 An Introduction to the Kryo1060 About this Manual Welcome to the Kryo1060, a system that provides precision cooling and heating of biological specimens. This manual is aimed at the Kryo1060-180 and Kryo1060-380 users who are responsible for operating or installing the system.

  • Page 12: The Kryo1060 Components

    An Introduction to the Kryo1060 The Kryo1060 Components The major components of the Kryo1060 system are shown in the following diagram. The diagram shows the Kryo 1060-380 freezer. PC running DeltaT Printer Kryo1060-380 Chamber Liquid-Nitrogen Delivery System Controller...

  • Page 13: Kryo1060 Chamber Controls

    An Introduction to the Kryo1060 Kryo1060 Chamber Controls Solenoid Valve Selector Valve A Valve B Manual Override Valve Main Door Handles Mains On/Off Thermocouple Entry Port Controller The diagram shows the Kryo1060-380 freezer.

  • Page 14: Controller - Front Controls

    An Introduction to the Kryo1060 Controller - Front Controls Chamber temperature (or sample temperature while sample button is depressed). EEEE indicates that the chamber or sample PRT is faulty. A profile -120.4 can be run without a working sample PRT but the chamber PRT must be in full working order.

  • Page 15: Controller - Rear Controls

    An Introduction to the Kryo1060 Controller - Rear Controls Mains inlet Press to force an increase in temperature Press to force a reduction in temperature Reset the controller Auxilliary connector for service calibration only RS232 Connection to PC 37 Way D Type Connector for Chamber Connection...

  • Page 16: The Kryo1060 Freezer

    An Introduction to the Kryo1060 The Kryo1060 Freezer The Kryo1060 freezer has many important design features: Precision temperature control - Accurate linear cooling and heating is achieved by the controlled injection of atomised liquid nitrogen into the chamber and the pulsing of a heater. A fan system and well-designed ducting ensure uniform circulation of nitrogen gas past the specimens and a uniform temperature throughout the freezing chamber.

  • Page 17: Personal Computer (Pc)

    An Introduction to the Kryo1060 Manual override valve - In the event of a total control failure, a manual override valve is provided which can be used to manually introduce liquid nitrogen into the chamber. Safety features - The lid activates an interlock switch to switch off the heater, fan and liquid-nitrogen solenoid valves when it is opened.
  • Page 18 An Introduction to the Kryo1060...

  • Page 19: Installing The Kryo1060 System

    Installing the Kryo1060 System C H A P T E R 2 Installing the Kryo1060 System This chapter describes how to unpack and install the Kryo1060-380 system. Preparation The freezer is intended to be floor mounted on a sturdy surface able to carry the weight of the freezer easily.
  • Page 20 Installing the Kryo1060 System Lifting equipment should only be used by suitably qualified persons trained in its use. Ensure that the equipment is suitable for supporting the weight of the Kryo1060. Refer to local Health and Safety regulations before proceeding. As described above, position the freezer as close as possible to its final location whilst still mounted on its pallet.

  • Page 21: Installing The Freezer

    Installing the Kryo1060 System Installing the Freezer ƒ ƒ ƒ ƒ Connect the freezer Check that the mains voltage rating, shown on the right-hand side panel of the freezer, is correct for the local voltage. If incorrect, contact your supplier and do not continue to install the freezer.
  • Page 22 Installing the Kryo1060 System Connect the mains lead from the Controller Power outlet on the side of the freezer to the mains inlet on the Controller. Side panel of chamber Controller to mains wall power outlet Connect the 37 way plug from the Kryo1060 side panel to the rear of the Controller.
  • Page 23 Installing the Kryo1060 System Ensure that the mains switch on the front of the freezer is in the off position 'O'. Connect the mains power cable to the Mains Inlet on the side of the freezer and then plug into a suitable wall power outlet.
  • Page 24 Installing the Kryo1060 System...

  • Page 25: Operating The Kryo1060 System

    Operating the Kryo1060 System C H A P T E R 3 Operating the Kryo1060 System This chapter describes how to operate and maintain the equipment on a day-to-day basis. Freezing Samples The Procedure to Follow Never leave the Kryo1060 freezer unattended while running a profile.
  • Page 26 Operating the Kryo1060 System Attach the sample PRT to the required location. Replace the two inner lids, with the two stainless steel strips positioned down the centre of the freezer and then shut the main door. Press down on the two main door handles until they latch in place.

  • Page 27: Stopping A Run

    Operating the Kryo1060 System ƒ ƒ ƒ ƒ Switch off the freezer Switching off the chamber at sub-zero temperatures may cause serious damage to the equipment. Switch off the freezer. Stopping a Run At any time, you can stop a run prematurely by pressing the STOP button on the front panel of the Controller.

  • Page 28: Dealing With A System Failure During A Run

    Operating the Kryo1060 System Dealing with a System Failure During a Run Although the Kryo1060 freezer has been rigorously designed and tested to provide utmost reliability, it is recognised that a fault could cause a run to stop prematurely. ƒ ƒ ƒ ƒ To deal with a system failure The first task is to protect the samples if at all possible.

  • Page 29: Routine Maintenence

    Operating the Kryo1060 System Note. Only use the Manual Override Valve if you have an independent means of monitoring the temperature within the chamber and never cool below -100 C. Following a system lockup, you need to reset the freezer's controller.

  • Page 30: Disposal

    Operating the Kryo1060 System Portable Appliance Tester or similar equipment, to ensure adequate earth bonding. P The earth continuity of the mains installation must also be regularly inspected by the person responsible for the safety of the installation. Disposal Do not dispose of with general waste. Ensure that the system has been cleaned as necessary to ensure that it is safe to handle and service, and is free from any biohazard...

  • Page 31: Chapter 4 Troubleshooting

    Troubleshooting C H A P T E R 4 Troubleshooting Kryo1060 Freezer ƒ ƒ ƒ ƒ Fan not running, RUN lamp fails to light when RUN pressed P Faulty mains supply. Remedy: check that the supply cable is properly connected and that the external fuse (if any) is intact.
  • Page 32 Troubleshooting ƒ ƒ ƒ ƒ Fan running, RUN lamp fails to light when RUN pressed P Control board latched up. Remedy: press reset button on rear of Controller or switch off and then on. P Blown mains input fuses in Controller. Remedy: check/replace as required with identically rated fuses.
  • Page 33 Troubleshooting No liquid-nitrogen supply Remedy: 1. Check supply valve is turned on. 2. Check supply hose for leaks or blockages and replace or rectify as necessary.
  • Page 34 Troubleshooting...

  • Page 35: Appendix A Kryo1060 Specifications

    Kryo1060 Specifications A P P E N D I X A Kryo1060-380 Specifications Kryo1060-380 Freezer Note These specifications are subject to change without notice. Dimensions External Internal Height 112cm 64cm Width 132cm 2 x 50cm Depth 116cm 50cm Weight 423kg (shipping weight including packaging) approx. Capacity 8000 x 2ml ampoules Circulation...
  • Page 36 Operating humidity Less than 90%. Altitude up to 2000 m Pollution deg. Pollution degree 2 (BS EN 61010-1) Vibration Designed to survive normal shipment when packed in the Planer supplied container. Not designed to endure significant vibration when being operated.

  • Page 37: Kryo1060-180 Freezer

    Kryo1060 Specifications Kryo1060-180 Freezer Note These specifications are subject to change without notice. Dimensions External Internal Height 112 cm 64 cm Width 86 cm 50 cm Depth 116 cm 50 cm Weight 211kg (shipping weight including packaging) approx. Capacity 4000 x 2ml ampoules Circulation Horizontal laminar flow.
  • Page 38 Operating humidity Less than 90%. Altitude up to 2000 m Pollution deg. Pollution degree 2 (BS EN 61010-1) Vibration Designed to survive normal shipment when packed in the Planer supplied container. Not designed to endure significant vibration when being operated.

  • Page 39: Aux Connector

    Kryo1060 Specifications AUX Connector 8 NC 7 NC 6 Calibration switch 5 NC 4 NC 3 0V (GND) 2 0V (GND) 1 NC Screen GND This connector is provided for calibration by qualified service personnel only. Do not connect to any other equipment.
  • Page 40 Kryo1060 Specifications...

  • Page 41: Appendix B Spare Parts

    When ordering spares, always quote the model number, the serial number, the part number and the part description. Always use qualified parts from Planer plc, except where parts are clearly marked with the original manufacturer's description and are identical to the original.
  • Page 42 Spare Parts...

This manual is also suitable for:

Kryo 1060-380Kryo 1060-180

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