Related Manuals for Gilson PIPETMAN P Series
Summary of Contents for Gilson PIPETMAN P Series
- Page 1 PIPETMAN ® ENGLISH User's Guide téléchargeable sur www.gilson.com auf www.gilson.com herunterladen scaricabile dal sito www.gilson.com se puede telecargar desde www.gilson.com...
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Page 2: Table Of Contents
14 - sPaRe PaRTs eC DeCLaRaTION Of CONfORMITY 1 - IntrodUctIon The Pipetting Standard – Gilson's PIPETMAN P is designed and manufactured to provide you with a range of robust, accurate and precise pipettes. The PIPETMAN P is a fully adjustable, air-displacement pipette with the selected volume shown on a digital indicator (volumeter). -
Page 3: Parts Check List
P5000, P10ml: measurement and transfer of large volumes. 2 - Parts check LIst Just take a moment to verify that the following items are present: - PIPETMAN P, - User's Guide, - Safety bag, - Certificate of conformity (including bar-code sticker). -
Page 4: Setting The Volume
PIPETMAN ® 4 - settInG the VoLUme The volume of liquid to be aspirated is set using the vol- umeter. The dials are colored either black or red to indicate 1.25 µL 7.5 µL 12.5 µL the position of the decimal point, depending on the P100 P200... -
Page 5: Pipetting
However, PIPETMAN P can also be used with the main tip brands. Fitting the tips To fit a new Gilson DIAMoND Tip, push the tip-holder into the tip using a slight twisting motion to ensure a firm and airtight seal. - Page 6 PIPETMAN ® Dual-position adapter for P2 and P10 with DL10 with D10 with D10 Pre-rinse the tips Some liquids (e.g. protein-containing solutions and organic solvents) can leave a film of liquid on the inside wall of the tip ; pre-rinse the tip to minimize any errors that may be related to this phenomenon.
- Page 7 First stop Second stop dispense Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°). Press the push-button slowly and smoothly to the first stop. Wait for at least a second, then press the push- button to the second stop to expel any residual liquid from the tip.
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Page 8: General Guidelines For Good Pipetting
PIPETMAN ® 6 - GeneraL GUIdeLInes For Good PIPettInG Table - Immersion Depth Make sure that you and Wait Time operate the push- button slowly and Model Immersion Wait Time Depth (mm) (seconds) smoothly. When aspirating, keep the tip at a P100 constant depth P200... -
Page 9: Accessories
ISO 8655-2 standard for conditions of use). 7 - accessorIes To make pipetting more comfortable and more secure, Gilson has developed several accessories: To avoid the possibility of liquid running back into the pipette, store the pipette vertically. CaRROUseL™ Pipette stand (7 pipettes) f161401 sINGLe™... -
Page 10: Glp Features
9 - troUbLeshootInG A quick inspection of the pipette may help you to detect a problem. You may download from the Gilson website (www.gilson. com) the “2 minute inspection”, which shows how to perform a quick diagnosis of your pipette. - Page 11 Damaged or corroded piston(s) Return pipette to supplier Damaged tip-holder(s) Replace the tip-holder Worn O-ring or seal Replace both parts Tips fall off or Low quality tips Use Gilson Diamond tips do not fit Damaged tip-holder(s) Replace the tip-holder correctly Damaged tip-ejector Replace tip-ejector However, if you can’t solve the problem, contact...
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Page 12: Leak Test
After making sure that the pipette is correctly reas- sembled, repeat this test. For the P2 to P200 models: Fit a Gilson DIAMoND Tip. Set the pipette to the maximum volume given in the specifications, and pre-rinse. Aspirate the set volume from a beaker of distilled water. -
Page 13: Maintenance
After replacing any parts you should verify the performance of your pipette following the verification procedure available on the Gilson website (www. gilson.com). If the pipette needs to be readjusted, please contact your local Gilson authorized Service Center. changing the tip-ejector... - Page 14 PIPETMAN ® Remove the piston assembly, o-ring and seal. Clean, autoclave, or replace the tip-holder. Reassemble the pipette (refer to the figure, p 14). Tighten the connecting nut (turn clockwise). Refit the tip-ejector (see above). Color coded push-button Connecting nut Piston assembly Seal O-ring...
- Page 15 Piston assembly You may remove the piston assembly for cleaning purposes only. If the piston assembly is changed, the pipette must be adjusted and calibrated in a Gilson authorized Service Center. The piston assembly must not be autoclaved.
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Page 16: Cleaning And Decontamination
Gilson authorized Service Center. You may refer to the decontamination procedure available on the Gilson website (www. gilson.com). Liquid must never enter the upper part (handle) of any pipette. cleaning The pipette must be cleaned, as described below, before it is decontaminated. - Page 17 Clean the individual components of the lower part of the pipette using an ultrasonic bath (20 minutes at 50°C) or with a soft-cloth and brushes. Small round brushes with soft plastic bristles may be used to clean the interior of the tip-holder. Rinse the individual components with distilled water.
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Page 18: Specifications
PIPETMAN P is a high quality pipette that offers excel- lent accuracy and precision. The figures given in the “Gilson Maximum Permissible Errors” table (page 19) were obtained using Gilson DIAMoND Tips. These figures are guaranteed only when genuine Gilson DIAMoND Tips are used. - Page 19 Gilson maximum Permissible errors Single channel Maximum Permissible Errors Model Volume Gilson ISO 8655 (Reference) (µL) systematic Random systematic Random error (µL) error (µL) error (µL) error (µL) ± 0.024 0.012 ± 0.08 0.04 ± 0.025 0.012 ±...
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Page 20: Spare Parts
PIPETMAN ® 14 - sPare Parts Service Kit 1st level includes: - 3 piston seals - 3 o-rings - 1 tip-holder Service Kit 2nd level includes: - 1 push-button - 1 connecting nut - 1 tip-ejector or only for the P2 and P10 models - 1 tip-ejector and 1 adapter... - Page 21 P2 (F144801) and P10 (F144802) Description C+D+E Service Kit 1st level F144501 F144502 A+B+F1+F2 Service Kit 2nd level F161970 F161971 Seal + O-ring (5 sets) F144861 F144862 Tip-ejector Adapter F144879 F144879 P20 (F123600) and P100 (F123615) Description P100 C+D+E Service Kit 1st level F144495 F144496 A+B+F...
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Page 22: Ec Declaration Of Conformity
PIPETMAN ® ec decLaratIon oF conFormIty The company, GILson s.a.s. 19, avenue des Entrepreneurs BP. 145 - 95400 Villiers-le-Bel (France) Tel.: +33(0) 1 34 29 50 00 Fax: +33(0) 1 34 29 50 20 www.gilson.com Hereby certifies on its sole responsibility that the products listed below: PIPetman®... - Page 23 notes...
- Page 24 LT801120/A - ©2009 Gilson SAS All rights reserved January 2009 English Printed in France Specifications subject to change without notifications - errors omitted. www.gilson.com sales@gilson.com service@gilson.com training@gilson.com Gilson, Inc. World headquarters 3000 Parmenter Street P.O. Box 620027 Middleton, WI 53562-0027, USA...
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