Nicoya OpenSPR Operation Manual page 31

7. The sensor chip should be rinsed with distilled/deionized water, then dried with clean
compressed air or nitrogen (both the front and back of the chip). It is important that the
sensor chip be completely dry for it to make a proper seal with the flow cell.
8. Carefully slide the sensor chip into the slot in the top of the sensor holder such that the
cut corner will be in the bottom right position when viewing the side of the holder
containing the slot [Figure 2.31]. If installed correctly, the glass chip will cover the entire
window, and the sensor will be fully seated into the bottom of the holder.
9. Place the holder containing the sensor into the docking stage. Ensure it is fully seated
within the docking stage.
10. Close the lid of the OpenSPR, then click "Dock" to allow the sensor to be automatically
docked into place, creating a seal with the flow cell.
11. When the sensor is docked, click "Next" in the active prompt [Figure 2.28]. This will begin
flow of the running buffer into the flow cell. This process will take about 2 minutes to
complete, and a progress bar and timer will inform you of the time remaining [Figure
2.32].
12. When the filling flow cell process is complete, the software will automatically check for
the detection of a sensor signal. A prompt will appear with the appropriate status [Figure
2.33].
OpenSPR 1-Channel Operation Manual V4.1.01
Figure 2.31 Proper installing of a sensor chip into the sensor holder.
Figure 2.32 Progress bar for filling flow cell with running buffer.
31