Resolve Automatic Check Errors - illumina NextSeq 500 System Manual

NextSeq 500 System Guide
RTA Errors
To troubleshoot RTA errors, first check the RTA error log, which is stored in the RTALogs folder. This file is not
present for successful runs. Include the error log when reporting issues to Illumina Technical Support.

Resolve Automatic Check Errors

If errors occur during the automatic check, use the following recommended actions to resolve the error.
If a pre-run check fails, the reagent cartridge RFID is not locked and can be used for a subsequent run.
However, the RFID is locked after the foil seals have been pierced.
System Checks
Doors Closed
Consumables Loaded
Required Software
Instrument Disk Space
Network Connection
Network Disk Space
Temperature
Temperature
Temperature Sensors
Fans
Imaging System
Imaging Limits
Z Steps-and-Settle
Bit Error Rate
Flow Cell Registration
Reagent Delivery
Valve Response
Pump
Buffer Mechanism
Spent Reagents Empty
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
Recommended Action
Make sure that the compartment doors are closed.
Consumable sensors do not register. Make sure that each consumable is properly
loaded.
On the run setup screens, select Back to return to the loading step, and repeat run
setup.
Critical components of the software are missing.
Perform a manual software update to restore all software components.
The instrument hard drive does not have sufficient disk space to perform a run. It is
possible that data from a previous run did not transfer.
Clear run data from the instrument hard drive.
The network connection has been interrupted. Check network status and the physical
network connection.
Either the BaseSpace account is full or the network server is full.
Recommended Action
Contact Illumina Technical Support.
Contact Illumina Technical Support.
Contact Illumina Technical Support.
Recommended Action
Contact Illumina Technical Support.
Contact Illumina Technical Support.
Contact Illumina Technical Support.
It is possible that the flow cell is not properly seated.
• On the run setup screens, select Back to return to the flow cell step. The imaging
compartment door opens.
• Unload and reload the flow cell to make sure that it is seated properly.
Recommended Action
Contact Illumina Technical Support.
Contact Illumina Technical Support.
Contact Illumina Technical Support.
Empty the spent reagents container and reload the empty container.
30