Zeiss LSM 880 with AiryScan FAST Training Manual page 33

Aligning AiryScan
The initial alignment of the AiryScan must be checked each time you change your sample. Slight variances in sample prep,
coverslip thickness and mounting media volume will influence the alignment so it is critical that this be checked for each new
sample.
The alignment only requires a single track for imaging. Select only one
track where the image has enough detail and contrast – please do not
attempt to use channels where the fluorescence is diffuse.
1. Go to Maintain tab in the main toolbar and undock the Airyscan
dialog. Place this somewhere off to the side of the image display.
2. Expand 'Airyscan' tab from image display toolbar.
3. Ensure the detector view is enabled from Airyscan options.
4. Click 'Continuous' to scan (do not use 'Live' for alignment).
Note: The AiryScan alignment is an automatic process that optimizes
during continuous scanning. You do not need to interact with this
alignment while the scan is going.
33 – LSM 880 Training Guide