Microtec CUT4060E Operating Manual

Rotary microtome
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® ® ® ®
microTec
R
M
CUT4060E
OTARY
ICROTOME
Operating Manual
MADE IN GERMANY
microTec
Laborgeräte GmbH
Rudolf-Diesel-Str. 47
69190 Walldorf, Germany
Phone: + 49 (0) 6227 8222-0
Fax: + 49 (0) 6227 8222-30
mail@micro-tec.de
www.micro-tec.de
1

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Summary of Contents for Microtec CUT4060E

  • Page 1 ® ® ® ® microTec CUT4060E OTARY ICROTOME Operating Manual MADE IN GERMANY microTec Laborgeräte GmbH Rudolf-Diesel-Str. 47 69190 Walldorf, Germany Phone: + 49 (0) 6227 8222-0 Fax: + 49 (0) 6227 8222-30 mail@micro-tec.de www.micro-tec.de...
  • Page 2: Table Of Contents

    ® ® ® ® microTec Table of Contents General Notes ………………………………………………………………… 4 Significance of the Operating Instructions ……………………………………4 Usage According to its Specification ………………………………………… 5 Improper Use ..................... 5 Warranty and Liability …………………………………………………………. 6 Warranty ………………………………………………………………………… 6 Brief Discription of Use, Function and Sequence ………………………….. 7 Edition, Date of Issue of the Operating Manual …………………………….
  • Page 3 ® ® ® ® microTec Operation ………………………………………………………………………. 22 Preparation, Orientation and Alignment of Specimen ………………………22 Commencing the Cutting Operation …………………………………………. 22 Instructions for Producing Sliced Microtomic Sections of the Specimen ... 22 Cutting Action …………………………………………………………………... 23 Handwheel ……………………………………………………………………… 23 Coarse Advanced Drive ………………………………………………………..23 Friction Clutch on the Coarse Advanced Drive Mechanism ……………….
  • Page 4: General Notes

    ® ® ® ® microTec 1. General Notes Significance of the Operating Instructions Dear Customer, Please read through the operating instructions carefully before using the equipment and familiarize yourself with its functions and controls. This is imperative to ensure proper use of the equipment.
  • Page 5: Usage According To Its Specification

    ® ® ® ® microTec Usage According to its Specification The CUT 4060E rotary microtomes are manually operated instruments for use in all work involving paraffin wax and solid cut microtomology within the disciplines of biology and medicine, and within industry at large.
  • Page 6: Warranty And Liability

    ® ® ® ® microTec Warranty and Liability The company – microTec GmbH – certifies that this instrument has been carefully tested and inspected. Verification to ensure adherence to the technical specification data published has been carried out prior to shipping out the instrument from the factory.
  • Page 7: Brief Discription Of Use, Function And Sequence

    ® ® ® ® microTec Brief Description of Use, Function, and Sequence The CUT 4060E rotary microtomes are manually operated instruments for use in all work involving paraffin wax and solid cut microtomology within the disciplines of biology and medicine, and within industry at large.
  • Page 8: Edition, Date Of Issue Of The Operating Manual

    ® ® ® ® microTec Edition, Date of Issue of the Operating Manual Edition: Version 004.96 Date of issue: 13. February 1996 Copyright Copyright : microTec Laborgeräte GmbH Copyright to this operating manual remains with microTec GmbH. This operating manual is only intended for use by the operator and his staff.
  • Page 9: Basic Safety Instructions

    ® ® ® ® microTec 2. Basic Safety Instructions Heed the Notes and Instructions Given in this Operating Manual! A prime prerequisite for safe handling and malfunction-free operation of this instrument is the awareness and recognition of basic safety instructions and regulations.
  • Page 10: Hazards And Dangers In Handling And Using The Instrument

    ® ® ® ® microTec Hazards and Dangers in Handling and Using the Instrument Important! The following precautionary measures must be undertaken during any operation carried out using the instrument. Non-compliance with these precautionary measures will infringe recognized principles and rules of technology and the intended and proper use of the instrument.
  • Page 11: Interpretation Of Hazard Symbols

    ® ® ® ® microTec Interpretation of Hazard Symbols Interpretation Symbol Danger • Warning • Caution • Note • Important ! • Information •...
  • Page 12: Informal Instructions Issued By The Manufacturer

    ® ® ® ® microTec Informal Instructions Issued by the Manufacturer Preparation of the Specimen Due attention should be paid in the preparation of specimens with regard to making the correct choice of the medium used for embedding and encapsulation of the specimen and its curing and infiltration time.
  • Page 13: Servicing And Fault Clearance

    No modifications, conversions or enhancements may be made to the instrument • without the approval being given by the manufacturer. All conversions require written confirmation issued by microTec. • Replace components which are not in proper working order immediately. •...
  • Page 14: Cleaning And Associated Care

    ® ® ® ® microTec 2.10. Cleaning and Associated Care Cleaning Depending upon the level of dirt and contamination and upon the frequency of use, regular cleaning and care of the rotary microtome is recommended. The instrument is best cleaned using a mild domestic cleaning agent.
  • Page 15: Transportation And Storage

    ® ® ® ® microTec 3. Transportation and Storage Dimensions and Weights Dimensions Width: .…….…… ……………. 460 mm Depth: ….……...…………….… 460 mm Height: ..…………………..…….. 280 mm Weight Weight (without accessories) ……..48 kg Instructions for Transportation Lock the handwheel. Remove all knives, knife holders, specimen...
  • Page 16: Data And Information Concerning The Instrument

    ® ® ® ® microTec 4. Data and Information Concerning the Instrument Designation Rotary Microtome, model CUT 4060E Scope of Supply, i.e. According to order confirmation.
  • Page 17: Packaging

    ® ® ® ® microTec Packaging Step Remove all side and top sitting screws sides and take the complete cover of the transportation crate. Step Remove all hexagon headed screws around and the packaging frame. Step With both hands lift the instrument out from the transportation crate.
  • Page 18: Ambient Temperatures During Operation

    ® ® ® ® microTec Ambient Temperatures during Operation + 10° C to +35° C Contamination level 2 Overvoltage categorie II (CUT 4055E & CUT 4060E) Performance Range of section thickness: 0.5 - 60 µm Increments: 0.5 µm from 0.5 µm to 2 µm 1 µm from 2 µm to 10 µm...
  • Page 19: Installation, Assembly, Commissioning

    ® ® ® ® microTec 5. Installation, Assembly, Commissioning Installation Protect the instrument from: • sources of heat such as fan heaters or hot air outlets ; • extremely low temperatures; • direct sunlight; • excessive dust; • moisture or rain;...
  • Page 20: Controls, Indicators And Displays

    ® ® ® ® microTec Controls, Indicators, and Displays Item Description Handwheel for cutting operation Handwheel handle for handwheel 1 Handwheel locking device Handwheel control panel Rotary knob for setting the thickness of slice Inspection window for setting and inspecting slice...
  • Page 21: Operating Panel

    ® ® ® ® microTec 5.3.1 Control Connect the operating panel as shown on the previous pages. If supplied, you may as well connect the following accessories: Foot switch: Order No. H150078 (optional on request) Inching service Upon pushing the „Inching service“ key, the vertical object fixture will carry out a cutting action.
  • Page 22: Operation

    ® ® ® ® microTec 6. Operation Preparation, Orientation, and Alignment of the Specimen Prior to starting to cut the specimen, the specimen must be suitably prepared and embedded and encapsulated in an appropriate medium. Various molds and plastic cassettes are used for encapsulation purposes.
  • Page 23: Cutting Action

    ® ® ® ® microTec Cutting Action The cutting action of the rotary microtome is generated by rotating the hand wheel in a clockwise direction. The cut is accomplished on the down stroke and retraction on the upwards directed stroke by further rotation of the hand wheel in a clockwise direction.
  • Page 24: Friction Clutch On The Coarse Advanced Drive Mechanism

    ® ® ® ® microTec Friction Clutch on the Coarse Advanced Drive Mechanism On reaching the front and rear positions of the specimen feed, a friction clutch built into the coarse advanced drive prevents the blocking of this coarse advanced drive. As soon as an audible engagement is triggered by the coarse drive, the feeding operation of the specimen stops.
  • Page 25: Setting Up And Changing The Setup

    ® ® ® ® microTec 7. Setting Up and Changing the Setup Specimen Fixture To hold the specimens during cutting, various adapters and clamping fixture systems are available, based on the microTec principle of modular construction. In conjunction with the specimen orientation device, it is possible to orientate and align the specimen to the plane of cut is simple manner.
  • Page 26: Orientatable Adapter

    ® ® ® ® microTec Orientable Adapter The orientable adapter can optionally be attached by screws to the various specimen clamping systems and be used only in combination with the specimen orientation device. Specimen clamping systems of orientable design are supplied ex works equipped with the orientable adapter.
  • Page 27: Cylindrical Specimen Insert And Prism

    ® ® ® ® microTec Cylindrical Specimen Insert and Prism To clamp cylindrical specimens, the cylindrical specimen insert, or the prism, can be inserted into the standard specimen clamping fixture, having the diameters - Ø 6 mm - Ø 15 mm - Ø...
  • Page 28: Film (Thin Material) Clamping Fixture

    ® ® ® ® microTec 7.6 Film (Thin Material) Clamping Fixture The film clamping fixture is a clamping system for film and foil materials or flat and thin specimens. To insert the specimen, the three threaded pins are loosened, and the movable jaw against the two compression springs, the setting of which is made by the two threaded pins, moved laterally.
  • Page 29: Specimen Orientation Device

    ® ® ® ® microTec Specimen Orientation Device Specimen clamping systems are attached to the orientation device by means of the orientable adapter. This way, orientation of the specimen in relation to the plane of cut is accomplished. Rotate the eccentric cam lever towards the front.
  • Page 30: Exchanging The Specimen Clamping Systems

    ® ® ® ® microTec Exchanging the Specimen Clamping Systems Exchanging the specimen clamping system Release (press downwards) the eccentric cam lever. Withdraw from the left out of the specimen orientation device. Screw out the orientation screws and spring loaded pin little way.
  • Page 31: Setting Up The Knife

    ® ® ® ® microTec 8. Setting up the Knife Knife Holder The knife holders are user friendly designed and easy to clean due to its special surface treatment. The risk of injury during the setting up of the knife and the specimen is minimized by a laterally movable finger guard.
  • Page 32: The "R" Type Knife Holder

    ® ® ® ® microTec The “R“ type knife holder Assembling the “R“ type knife holder on the base of the knife holder Release clamping lever in opposite direction to that of the arrow. Introduce the “R“ type knife holder into the clamping mechanism.
  • Page 33: The "Rs" Type Knife Holder

    ® ® ® ® microTec The “RS“ type knife holder Stable version for Tungsten carbide conventional steel knives with the central clamping plate providing optimal stability. The knife is stabilized exactly, where the highest cutting forces are applied. Full use of the knife length Assembling the „RS“...
  • Page 34: Holder For Throw-Away Blades

    ® ® ® ® microTec Holder for Throw-away Blades Assembling the holder for throw-away blades on the base for the knife holder. Release clamping lever in opposite direction to that of the arrow. Introduce the holder of the throw-away blades into the clamping mechanism.
  • Page 35: Clearance Angle Adjustment

    ® ® ® ® microTec Utilization of the blades In order to utilize the entire cutting edge of the blades. These blades can be pushed to the right or to the left. Release clamping lever. Push blade with the clamping plate to the left or the right.
  • Page 36 ® ® ® ® microTec Notice...

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