Related Manuals for VistaLab Ovation
Summary of Contents for VistaLab Ovation
- Page 1 Operator’s Guide for Electronic Single hannel Macro Volume Pipettes 0.2-10mL 0.2-10mL L065-0001-001 Rev. F...
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Page 2: Table Of Contents
Battery Information Troubleshooting Warranty, Safety Compliance & Contact Information Appendix A Hazards & Precautions Operating Temperature & Environment Conditions Power Supply and Battery Specifications Chemical Compatibility Tips & Accessories Appendix B Ovation BioNatural Pipette – Electronic Single Channel Operator’s Guide... - Page 3 This manual describes how to use and care for your Ovation electronic single channel or macro pipette. As you can see from its appearance, it is different from every pipette that you have used before, therefore please read the instructions carefully.
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Page 4: Pipette Components
AC adapter for recharging battery (not shown) Captive Screw When loosened, allows access to inside of pipette for battery replacement Serial Number Provides positive identification for the pipette Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 1 - 2... -
Page 5: Bionatural Pipetting
The Ovation BioNatural Pipette is the only pipette designed to keep your hand in the neutral position recommended by ergonomics experts. We call this BioNatural™... - Page 6 2. Minimized exertion and contact stress 3. Low contact pressure 4. Lowest pressure in carpal tunnel and to median nerve 5. Minimized tension to shoulder and elbow Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 2 - 2...
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Page 7: Selecting Liquid Handling Functions
Press down until you hear or feel a “click”. This “click” indicates that the tip is properly seated and ready for use. Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 2 - 3... -
Page 8: Aspirating And Dispensing
Raising the arm to discard tips into a tall receptical on the counter may negate some of the benefits of Ovation’s low-profile design. Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 2 - 4... - Page 9 Store nozzle in a safe place. Nozzle 2. Carefully screw on adapter (clockwise). Ensure adapter is square to threads for proper installation. Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 2 - 5...
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Page 10: Operating Procedures
Displayed when cal factor has Icon indicates Displays liquid handling been changed from factory remaining function, setting or prompt setting of 1.000 battery capacity that is active Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 3 - 1... - Page 11 The volume of individual dispense(s) when setting up the MULTI function. The volume of the dilution to be mixed when using the SDILU function. The volume to be mixed when using the MIX function. Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 3 - 2...
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Page 12: Set-Up Of Liquid Handling Functions
Set-up of Liquid Handling Functions Introduction The Ovation pipette’s keypad is easy to use and provides access to all liquid handling functions and settings. Simply press the buttons on the keypad to step through the various functionss and make changes to settings. When a setting indicator is flashing, this indicates that the setting has been changed from a previously “saved”... - Page 13 Liquid Handling Functions Pipette Ovation pipettes provide user-defined pre-sets for routine pipetting (TC or “to contain”) at exact volumes with automatic blow-out (P1, P2 & P3 on electronic single channel models, P1 & P2 on macro models). An additional pipetting function (...
- Page 14 Multiple Multiple Dispense is a repetitive pipetting function for dispensing equal volumes. Once a dispense volume and the number of dispenses are established, the Ovation Dispense pipette automatically calculates the total fill volume required. To set-up and run a Multiple Dispense liquid handling function, follow these steps: Example: Dispense a 50µL aliquot, 10 times...
- Page 15 13. To empty the tip of liquid after the final dilution is made, press appears on the display (or to cancel PURGE, press again). Press and release the pipetting trigger to dispense liquid into an appropriate container. Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 3 - 6...
- Page 16 9. When zero appears on the display, remove tips from solution and press the pipetting trigger. The pipette is now ready to perform the next Mix function. Z E R O 100 . 0 µ M I X 5 Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 3 - 7...
- Page 17 The P U R G E pipette is now ready to perform the next Reverse Pipette function. µ R ever Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 3 - 8...
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Page 18: Set-Up Of Pipette Settings
Set-up of Pipette Settings Ovation pipettes use “beep” tones to indicate various actions. A single beep tone Tone indicates the end of a pipetting step. A double beep tone indicates the completion of the (last) dispense cycle when using the multiple dispense or reverse pipette function. -
Page 19: Introduction
The Ovation pipette can be easily in-lab calibrated ±10% for optimum performance at your operating conditions. In addition, the calibration factor can be pre-determined and set at the appropriate value for a liquid to be dispensed. Use Ovation’s calibration software to quickly and easily determine an appropriate calibration factor. Returning the calibration factor to 1.000 will re-set the Ovation pipette to its original factory... -
Page 20: Entering A Known Calibration Factor
Record this value. To set the calibration factor for use with serological pipets: 1. In the P1, P2 or PDB liquid handling function, set the macro Ovation to the maximum volume setting (i.e. 5000µl or 10.00mL). 2. Aspirate water or the liquid to be used with the serological pipet. Using the graduations on the pipet and the meniscus of the liquid, determine the volume aspirated. - Page 21 7. Press and the Ovation Macro Pipette will automatically store this factor. A “C” may now appear on the display if the Cal factor is other than the factory setting of 1.000.
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Page 22: Cleaning Exterior
MAINTENANCE & TROUBLESHOOTING The Ovation pipette requires minimal maintenance. Always store it in its “standing” position or on the Ovation Pipette Stand (Catalog No. 9058-4003) when not in use. Cleaning Exterior Clean outer surfaces as needed with a soft cloth dampened with warm water. To decontaminate outer surfaces, wipe with a 70% aqueous solution of ethanol or isopropranol. -
Page 23: Replacing A Nozzle (Macro 5Ml And 10Ml)
2. Screw new nozzle into the ejector sleeve. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the pipette. Ovation BioNatural Pipette – Electronic Single Channel Operator’s Guide 5 - 2... -
Page 24: Replacing A Nozzle Filter (Single Channel)
9. Place rubber tubing onto the nozzle, and screw the nozzle onto the pipette in a clockwise direction. Firmly tighten and remove tubing from nozzle. Re-install ejector sleeve. Ovation BioNatural Pipette – Electronic Single Channel Operator’s Guide 5 - 3... - Page 25 8. Screw the nozzle onto the extension tube. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the extension tube. Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 5 - 4...
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Page 26: Replacing A Nozzle Filter (Macro 10Ml)
4. Screw nozzle (or new nozzle if replacing) into the ejector sleeve. Tighten by hand, or use a macro pipette tip to ensure the nozzle is firmly attached to the pipette. Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide 5 - 5... -
Page 27: Battery Information
The Ovation pipette’s battery must be recharged for approximately 90 minutes when General Battery the pipette is first received, or if the battery is fully discharged. To ensure the pipette Information has sufficient power for extended use, connect it to the power supply whenever it isn’t being used or when the battery icon on the LCD is flashing (indicating a low charge). -
Page 28: Troubleshooting
Probable cause: The remaining battery charge is low Recommended action: Plug AC power supply into Ovation and to an AC source. Begin to use and allow to recharge at a convenient time. Symptom: The pipette does not aspirate or dispense properly... - Page 29 Symptom: Pipette is leaking during pipetting Probable cause: Non-Ovation tips are being used, tip is not on securely, or inner piston or nozzle seal is worn Recommended action: Use Ovation tips. Tighten and/or replace the pipette nozzle. If it continues to leak, inner piston seal may need to be replaced.
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Page 30: Warranty, Safety Compliance & Contact Information
VistaLab Technologies, Inc. warrants the Ovation BioNatural Pipette against defects in materials and workmanship for one year from the date of purchase. To register your pipette and activate the warranty, register on the VistaLab Technologies web site at www.vistalab.com. This warranty is void if failure or damage is the result of improper handling, unauthorized modification, or use of ancillary products not supported by VistaLab Technologies. -
Page 31: Hazards & Precautions
Hazards and Proper use of Ovation pipettes as specified in this manual will ensure safe operation. However, please be aware of the following situations that can damage the device or cause Precautions serious personal injury. WARNING! Connect power supply to a compatible power source. Refer to the voltage configurations shown below. - Page 32 RNase/DNase certified, 192 tips/rack, 5 racks † trace amounts of carryover. 4060-1333 † 2µL tip for Ovation 0.2-20µL, Ovation 1µL and Ovation 2µL only 4. All 5mL tips are VistaClear Box – Filtered, Sterile, Pyrogen-free, graduated, marked at RNase/DNase certified, 60 tips/box...
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