SpectrumLabs Tangential Flow Depth Filtration Product Instructions For Use

Tangential Flow Depth Filtration
(TFDF )
®
Introduction
SpectrumLabs.com offers a unique TFDF
the benefits of the non-fouling nature of tangential flow filters
with the benefits of high dirt capacity depth filters. Please refer
to the following instructions for the care and use.
A. Materials of Construction
Housing: Polysulfone
Filter Media: HDPE 1-3um Pore Rating
HDPE 2-5um Pore Rating
Potting Material: Urethane
- ll materials USP Class VI compatible
-Pore Ratings for reference only: Retention rating may vary
based on application
B. Filter Preparation
TFDF ® filters are shipped ready-to-use with no surfactant or
storage agent. Filters wet readily with water and/or buffer.
C. Filter Sterilization
SpectrumLabs.com recommends using the filters with Pro-
onnex Ready-to-Use, Sterile Flow Paths that include the sin-
®
gle-use MagLev pump and pressure sensors for perfusion
applications. When needed, Sampler and MiniKros
ters part numbers beginning with S and N) filters can be auto-
claved one time using the conditions below:
NOTE
KrosFlo and KrosFlo
®
with K or X) have not been validated for autoclave
cycles.
1. Remove and discard all pouches, as these are not auto-
clavable; wet the membrane (optional).
2. Loosen all module connections to avoid damaging the
module during the autoclave cycle.
3. Wrap all open ports of the connecting tubing with auto-
clave paper, making sure permeate cavity is open to
steam penetration along with the inlet/retentate side.
4. Ramp up the autoclave by increasing the temperature by
1-2° /min up to 121° (15 psig)
®
filter that combines
®
® filters (fil-
Max filters (filters beginning
®
Product Instructions for Use
TANGENTIAL FLOW DEPTH FILTRATION
® ®
(TFDF ) p 1