Instrument Run Troubleshooting; Re-Extraction Troubleshooting - Applied Biosystems 3730xl User Manual

Instrument run troubleshooting

Observation
Plate validation fails when you
start a run
"Container and NumCap
validation failed" message in
Event Log
"Sealing type (from instr) is
Heat Sealing and does not
match set by user Septa"
message in Event Log
"Error getting plate info from
database" message in Event
Log

Re-extraction troubleshooting

Observation
Re-extraction fails
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
Possible cause
The dye set for the active
calibration does not match the
dye set specified in the
instrument protocol.
An object in the plate record
(instrument protocol, analysis
protocol, results group) has
been deleted from the
database.
Results group Analysis tab
specifies autoanalysis, but no
Login ID or Password is
specified.
The plate record specifies a
plate type (number of wells)
that does not match the plate
type that is detected by the
instrument.
The plate record specifies a
sealing type that does not
match the base in which the
plate is loaded.
After the plate record was
added to the Run Scheduler,
the plate record was deleted in
the Plate Manager.
Possible cause
You used an analysis protocol
that was previously used to
process the plate record (it was
deleted from the plate record,
then added back to the plate
record).
Unknown cause.
Appendix A Troubleshooting

Instrument run troubleshooting

Recommended action
Change the active calibration to match the dye
set that is specified in the instrument protocol.
Edit the results group to specify an instrument
protocol with a dye set that matches the active
calibration.
See also the Event Log message observations
listed below.
Edit the plate record.
Edit the results group.
Edit the plate record to match the plate type.
Edit the plate record to specify the sealing type
that matches the base in which the plate is
loaded.
Load the plate in the correct base. See
"Prepare the plate assemblies" on page 42.
Load a different plate record.
Recommended action
Use a new analysis protocol with the same
settings, or create a new plate record for re-
extraction.
Review the run log file. See "Review a run log
file" on page 135.
A
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