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VWR International VWR 10 Instruction Manual

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VWR
ELECTRONIC PIPETTE
INSTRUCTION MANUAL
North American Catalog Numbers
10827-884
VWR ELECTRONIC PIPETTE 10µL
10827-918
VWR ELECTRONIC PIPETTE 20µL
10827-920
VWR ELECTRONIC PIPETTE 300µL
10827-922
VWR ELECTRONIC PIPETTE 1200µL
10827-924
VWR ELECTRONIC PIPETTE 8X10µL
10827-926
VWR ELECTRONIC PIPETTE 8X20µL
10827-928
VWR ELECTRONIC PIPETTE 8X300µL
10827-930
VWR ELECTRONIC PIPETTE 8X1200µL
10827-932
VWR ELECTRONIC PIPETTE 12X10µL
10827-934
VWR ELECTRONIC PIPETTE 12X20µL
10827-936
VWR ELECTRONIC PIPETTE 12X300µL
10827-938
VWR ELECTRONIC PIPETTE 12X1200µL
European Catalog Numbers:
613-5415
613-5416
613-5417
613-5418
613-5419
613-5420
613-5421
VWR ELECTRONIC PIPETTE 8X300µL
613-5422
VWR ELECTRONIC PIPETTE 8X1200µL
613-5423
VWR ELECTRONIC PIPETTE 12X10µL
613-5424
VWR ELECTRONIC PIPETTE 12X20µL
613-5425
VWR ELECTRONIC PIPETTE 12X300µL
613-5426
VWR ELECTRONIC PIPETTE 12X1200µl
VWR ELECTRONIC PIPETTE 10µL
VWR ELECTRONIC PIPETTE 20µL
VWR ELECTRONIC PIPETTE 300µL
VWR ELECTRONIC PIPETTE 1200µL
VWR ELECTRONIC PIPETTE 8X10µL
VWR ELECTRONIC PIPETTE 8X20µL

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  • Page 1 ® ELECTRONIC PIPETTE INSTRUCTION MANUAL North American Catalog Numbers 10827-884 VWR ELECTRONIC PIPETTE 10µL 10827-918 VWR ELECTRONIC PIPETTE 20µL 10827-920 VWR ELECTRONIC PIPETTE 300µL 10827-922 VWR ELECTRONIC PIPETTE 1200µL 10827-924 VWR ELECTRONIC PIPETTE 8X10µL 10827-926 VWR ELECTRONIC PIPETTE 8X20µL 10827-928 VWR ELECTRONIC PIPETTE 8X300µL 10827-930 VWR ELECTRONIC PIPETTE 8X1200µL...
  • Page 2 Version: Issued: 02/18/2016 United States Europe VWR International, LLC VWR International bvba 100 Matsonford Rd Researchpark Haasrode 2020 Radnor, PA 19087 Geldenaaksebaan 464 800-932-5000 B-3001 Leuven http://www.vwr.com + 32 16 385011 http://be.vwr.com Country of origin: FRANCE Intended use : ®...

  • Page 3: Table Of Contents

    Table of Contents Page 1 - INTRODUCTION 4 2 - PARTS CHECK LIST AND ACCESSORIES 5 3 - GETTING STARTED 6 4 - DESCRIPTION 6 5 - PIPETTING 8 6 - PIPETTING MODES 8 7 - PIPETTING SPEED CONTROL 14 8 - GUIDELINES FOR GOOD PIPETTING 14 9 - PERSONALIZE YOUR VWR ELECTRONIC PIPETTE 16 10 - GOOD LABORATORY PRACTICE FEATURES 17...

  • Page 4: Introduction

    INTRODUCTION :  Ease-of-use: Only 2 buttons and 5 pipetting modes including the CUSTOM Mode which allows to create pipetting protocols easily and rapidly  Advanced Ergonomics: Lightweight and Perfectly Balanced, it requires virtually no force to significantly reduce RSI ...

  • Page 5: Parts Check List And Accessories

    Catalog Number Model Volume range Color code Europe VWR 10 10827-884 613-5415 0,5-10µl VWR 20 10827-918 613-5416 2-20µl Pale Yellow VWR 300 10827-920 613-5417 20-300µl Green VWR 1200 10827-922 613-5418 100-1200µl Blue VWR 8x10 10827-924 613-5419 0,5-10µl VWR 8x20 10827-926 613-5420 1-20µl...

  • Page 6: Getting Started

    GETTING STARTED VWR Electronic Pipette is provided with a minimal battery charge. Before using it, we strongly recommend you to fully charge the battery. VWR Electronic pipette charges 80 % of its full battery capacity in less than an hour; it takes three hours to fully charge the battery.
  • Page 7 Lower part: Tip-ejector: removable access to tip-holder Optimized tip-holder to reduce tip fitting and ejection forces, removable for cleaning and servicing Ejector clip Ejector support Cover Ejector spacer (only for Multi 1200µl) Screen: Pipetting Mode Aspirate and dispense indicator Battery status Purge indicator Volume...

  • Page 8: Pipetting

    Reset: To reset your pipette, simultaneously press on the Push-button and the Mode-button for at least 5 seconds. Switch off: To switch off your VWR Electronic Pipette, press the Push-button for at least 5 sec. Display: The VWR Electronic Pipette display is an OLED screen. It shows the current mode and operation step, pipetting volume, battery indicator and piston status (aspirated and dispensed volume) in real time.
  • Page 9 PIPET Mode This is the classic pipette mode (forward pipetting), your pipette is simply aspirating and dispensing. • Fit a suitable tip • Press the Push-button to aspirate the selected volume. • To dispense: Click (press and release) the Push-button. Three things happen: sample dispense, automatic purge and piston reset to zero.
  • Page 10 • Set the aliquot volume : Press the Push-button; the volume on the display will start to blink, you can now adjust the volume by turning left or right the Push-button. Then, click on the Push-button to confirm the volume choosen. The maximum number of aliquots has been calculated by your pipette.
  • Page 11 MIX Mode This is the classic pipette mode (forward pipetting) followed by a mixing phase, composed of repeatedly aspirating and dispensing, as well as an optional forward pipetting step. • Press the Mode-button to access menu, where you can choose between different pipette modes. Select “MIX Mode”...
  • Page 12 If you choose VOLUME1 = 0, step 2 won’t occur, if you choose VOLUME 2 = 0 μL, step 4 won’t occur, as well if you set VOLUME 1 and 2 = 0 μL, step 1-4 will disappear. If you set VOLUME 3 = 0 μL step 7 will not occur.
  • Page 13 CUSTOM Mode The CUSTOM Mode enables personalized pipetting protocol creation on a computer for transfer to VWR Electronic Pipette through VWR Protocol Builder software, an easy-to-use software. To use this mode, the software has to be installed on the computer (file downloadable on www.vwr.com/e-pipette).

  • Page 14: Pipetting Speed Control

    • Beep: makes pipette produce a beep sound while selected tasks are executed To transfer a protocol to your VWR Electronic Pipette, click the “Transfer custom protocols to pipette” button and follow the instructions on the screen. The CUSTOM mode is available in pipettes from firmware version cf01.00.03 For more information on VWR Protocol builder software, see page 34.
  • Page 15 Wait for at least a few seconds before releasing the Push-button to expel any residual liquid from the tip. While removing the pipette, draw the tip along the inside surface of the vessel. 5/ Eject the tip by pressing firmly on the tip-ejector button. General guidelines for good pipetting: a.

  • Page 16: Personalize Your Vwr Electronic Pipette

    PERSONALIZE YOUR VWR Electronic Pipette Tip-ejector extension for use VWR Electronic Pipette 10µl Single and Multi channels: In order to eject 10µl tips, tip-ejector extensions are supplied with VWR Electronic Pipette 10µl Single and Multi channels. The tip-ejector extension for Single channel which is made of PVDF (polyvinylidene Fluoride),is autoclavable, as well as the one of the Multi-channel which is in PBT.

  • Page 17: Good Laboratory Practice Features

    Personal label – Name and/or application tag: VWR Electronic Pipette offers you the possibility to mark your pipette. You can personalize your pipette with a name tag and/or an application tag: Window-tag: • Remove the window by inserting a small screwdriver in the access slot.
  • Page 18 Battery charging VWR Electronic Pipette charges 80 % of its full battery capacity in less than an hour, it takes three hours to fully charge the battery. 1/ Charge your pipette by using the power supply: - Connect the AC adapter to a suitable AC power supply (EU plug / UK plug….) - Plug the adapter into the Mini USB port of the pipette.

  • Page 19: Configuration

    You can also recharge VWR Electronic Pipette by USB with your computer; however it will take much longer than by power supply. Use only the original power transformer supplied by VWR. Use of an incompatible power supply can damage your VWR Electronic Pipette Low battery warning VWR electronic Pipette has a low battery alert.

  • Page 20: Maintenance Of Your Vwr Electronic Pipette

    3. Contrast: You can choose between values of 1 to 5 to set up the contrast of the screen. 4. Service: refer to chapter 13. 5. Adjustment: refer to chapter 13 6. Language: Available from the firmware version cf01.00.03. Languages available are English, Chinese and Japanese.
  • Page 21 b. Service info provides you with maintenance information; you cannot modify any of the items: • Number of pipetting cycles since last maintenance. • Number of weeks since last maintenance. c. Service settings gives you the option to organize your maintenance, you can set following service points: •...
  • Page 22 Conversion to volume must take into account the density of the liquid as well as evaporation during the cycle time. For each measurement, the corresponding volume (Vi) can be calculated as follows: Wi is the weight as read on the balance e is the mean evaporation loss during the cycle time Z expressed inL/mg, is a conversion factor incorporating density of water buoyed in air, at test temperature and barometric pressure (see Appendix II)
  • Page 23 the procedure.  Changing the tip-holder (lower part) After removing the tip-ejector, you may remove the lower part of the tip-holder, which is more likely to become contaminated or damaged than the upper part. Removal of the lower part is shown here; for the upper part see “Removing the piston”...
  • Page 24  Removing the piston 1/ Remove the tip-ejector and (optionally) the lower part of the tip-holder. If you remove the lower part, take care to remove the O-ring as described in “Changing the O-ring”. 2) Unscrew the connecting nut (turn by hand, counterclockwise). 3) Gently remove the connecting nut and upper tip holder.

  • Page 25: Cleaning And Decontamination

    How to lubricate the piston (except VWR10) Use only VWR lubricant (delivered inside the pipette box). Squeeze a small quantity from the tube onto a clean, lintless cloth. Use the cloth to transfer the lubricant to the piston. Ensure that the piston is evenly lubricated and that you wipe away any excess –...
  • Page 26 4/ To rinse, wipe the entire pipette with a soft-cloth or lint-free tissue impregnated with distilled water. 5/ Leave to air dry. Internal The following components only can be immersed in a cleaning solution: tip-ejector, tip-holder, connecting nut and piston. 1/ Disassemble the pipette as described in chapter 13 2/ Set aside the handle in a dry and secure location.

  • Page 27: Leak Test

    Chemical decontamination - Single and multichannel models You may choose to decontaminate your pipette chemically, in accordance with your own procedures. Whatever decontaminant you use, check that it is compatible with the plastics used in the construction of the pipette (see Chapter 14). Parts that may not be immersed 1/ Wipe the handle of the pipette with a soft-cloth or lint-free tissue covered with the chosen decontaminant.

  • Page 28: Troubleshooting

    • For multichannel models, check if the water level between each tip is the same TROUBLESHOOTING In case of malfunction, first reset the pipette by pressing on the Push-button and the Mode-button simultaneously at least for 8 seconds. If the problem persists, you may consult the following table which identifies potential problems and their solutions.

  • Page 29: Safety Precautions And Limitations Of Use

    • Ten measurements for each test volume, which are nominal volume, 50 % of nominal volume and 10 % of nominal volume. SAFETY PRECAUTIONS AND LIMITATIONS OF USE For safety reasons, it is important to observe the following instructions: 1. Battery and electrical specifications: Use AC-adaptor and stand adapter indoors.
  • Page 30 Dispose of used batteries in accordance with legal regulations. Batteries may not be disposed of with household waste and may explode if disposed in fire! We strongly recommend you fully charge the battery before using the pipette. VWR Electronic Pipette is supplied with an AC adapter that is suitable for your country.

  • Page 31: Replacement Parts

    REPLACEMENT PARTS COMING SOON...

  • Page 32: Appendix 1: Example Of A Performance Check

    PPENDIX 1 - EXAMPLE OF A PERFORMANCE CHECK (example with VWR10 at 1µl)

  • Page 33: Appendix 2: Z Factor

    APPENDIX 2 - Z FACTOR...

  • Page 34: Appendix 3: Evaporation Loss

    PPENDIX 3 - EVAPORATION LOSS Procedure for the determination of evaporation loss Use the same distilled water, weighing vessel and balance as you will be using for the gravimetric check. 1. Half fill the weighing vessel with distilled water. 2. Cover the weighing vessel with its lid and place it on the balance using a pair of tweezers. 3.
  • Page 35 VWR PROTOCOL BUILDER SOFTWARE Contents: 1. Introduction 1.1 What is VWR Protocol Builder Software 1.2 Passwords and user information 1.3 Forgotten password 2. Home screen 3. Installing VWR Protocol Builder Software 3.1 Computer requirements for VWR Protocol Builder Software 3.2 Installing VWR Protocol Builder Software 3.3 Installing the drivers 3.4 Software files 4.
  • Page 36 INTRODUCTION 1.1 What is VWR Protocol Builder Software? VWR Protocol Builder Software is an easy-to-use software for creating custom pipetting protocols for VWR Electronic Pipettes. Use it to create personalized pipetting protocols on your computer, and to transfer these protocols to VWR Electronic Pipettes in the Custom Mode, where you can execute them.
  • Page 37 And click Next. Provide the answer to the Hidden question or the Recovery password on the next screen and click Next. On the following window, provide a new password and click Finish. HOME SCREEN After launching VWR Protocol Builder Software you are directed to the home screen. The Home screen offers the following options: Manage custom protocols - Create, edit, import or export custom protocols.
  • Page 38 Installing VWR Protocol Builder Software 3.1 Computer requirements for VWR Protocol Builder Software VWR Protocol Builder Software is supported on PCs with Windows 7, Windows 8 and Windows 8.1. Table below lists the computer system requirements for the software. 3.2 Installing VWR Protocol Builder Software i.
  • Page 39 Creating and managing custom protocols In Manage custom protocols you can create, edit, import or export custom protocols. 5.1 Creating a custom protocol Start creating a new custom protocol from scratch by clicking the Create a new protocol button. Specify the Protocol name (e.g. DNA-isolation) and click Create. The edit custom protocol screen appears with the Protocol info and Protocol setup tabs.
  • Page 40 Default tasks available: • Aspirate: aspirates specified volume into the tip. • Dispense: dispenses specified volume from the tip. • Dispense all: dispenses all remaining volume from the tip followed by a purge action. • Repeat : enables repetition of any task once or more. •...
  • Page 41 Tasks within Repeat will be executed in a sequence for the specified number of iterations. Example: If Repeat contains two tasks: Aspirate 20 μL followed by Dispense 20 μL and the number of iterations is set to 3, the tasks will be executed on VWR Electronic Pipette in the following order: 1.
  • Page 42 • Use Push-button mixing (checkbox): when checked, mixing will be performed continuously for as long as the user holds the Push-button on the pipette; once the Push-button is released, mixing stops and the next task in the protocol is executed. •...
  • Page 43 5.1.2. Editing, cloning and deleting tasks: Once the task is listed in the protocol, you can edit its parameters by selecting the task, right-clicking on it and selecting Edit task, or by double-clicking the task. The Edit task window opens, where you can modify the task’s parameters and store them. You can clone a task by right-clicking on the desired task and selecting Clone task.
  • Page 44 5.1.4. Warning and error messages: VWR Protocol Builder Software verifies the protocol after the addition of each task, or after modification of a task, to determine whether the volume of the pipette is still within the volume specifications for that particular VWR Electronic pipette model. Should the volume in pipette model fall outside specifications, an error sign will appear next to the task that caused this.
  • Page 45 For each of the protocols listed you can perform the following actions. Simply select the protocol and click on one of the buttons: • View: view a protocol. No parameters can be changed in the “View mode”. • Edit: edit a protocol (change the VWR Electronic Pipette model, rename protocol, add/edit/ remove tasks).
  • Page 46 5/ You can also check “Mute beeps” if you want to disable any pipette sounds during beep tasks. 6/ Disconnect the pipette. The Custom protocol is now available on the pipette. Note: Protocols that have a red error sign cannot be transferred to pipettes. •...
  • Page 47 Updating pipette settings Here you can set the following configurations on the connected VWR Electronic pipette: • Set the Beep to On or Off (switches the tone of the pipette to On or Off). Beep On and Off only affects the built-in pipette protocols, as well as Aspirate, Dispense, Mix, Purge tasks (but NOT beep task!).
  • Page 48 5. Click Finish. 6. Disconnect pipette. You can refresh the pipette’s Maximal volume limit to pipette’s specified maximal volume by clicking the Reset to pipette max. Note: the pipette must be registered in the software prior to transferring custom protocols. If the connected pipette is not yet registered, a pop-up appears which informs you that the pipette is not yet registered.
  • Page 49 8.1 Pipette management: Here you can register your individual VWR Electronic pipettes. Registering a pipette is required to allow for transfer of your personalized protocols onto the pipette, as well as updating the pipette settings. Register a new pipette by clicking Register new pipette button and follow the instructions on the screen: 1.
  • Page 50 To display history of protocol modifications, navigate to Protocol management tab, select the time interval, and click Show history. The protocols that have been modified within selected time interval will be listed in the table below, displaying the following information for each protocol: protocol ID, Original protocol name, Modified by, Modified on, and Action.
  • Page 51 8.3 Change password: Here you can change a password, change the Hidden question and Hidden answer, or disable/enable the use of passwords (checkbox: Don’t use password). Note: remember or store the answer to the hidden question in case of password recovery 8.4 Institution/Company profile: Enter the information related to your Institution/Company here or modify it.

  • Page 53: Warranty

    Technical service Web Resources Visit the VWR website at www.vwr.com for: • Complete technical service contact information • Access to the VWR Online Catalogue, and information about accessories and related products • Additional product information and special offers Contact us for information or technical assistance contact your local VWR representative or visit.
  • Page 54 Equipment disposal This equipment is marked with the crossed out wheeled bin symbol to indicate that this equipment must not be disposed of with unsorted waste. Instead it's your responsibility to correctly dispose of your equipment at lifecycle -end by handling it over to an authorized facility for separate collection and recycling.
  • Page 55 Email: info@de.vwr.com Email: labart@pl.vwr.com *0,14 €/Min. aus d. dt. Festnetz Austria Portugal Hungary VWR International GmbH VWR International - Material de Laboratório, Graumanngasse 7 VWR International Kft. Edifício Neopark 1150 Vienna Simon László u. 4. Av. Tomás Ribeiro, 43- 3 D Tel.: +43 1 97 002 0...

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