Lonza Amaxa Nucleofector II Manual page 16

14
4.0 Nucleofector™ II Device
Error Codes
The following guide indicates possible Nucleofector™ Error Codes
and provides suggestions to solve the problem on your own. Should
these suggestions not resolve the problem, please call Lonza's
Scientific Support Team (Europe/World: +49 (0)221-99199-400;
USA: 800-521-0390). Alternatively use our internet and e-mail based
scientific support at www.lonza.com/nucleofection-support. If the
Nucleofector™ II Device has to be returned for repair, please contact
our Scientific Support Team for shipping and warranty instructions.
Arc discharge correction
Code
Arc discharge
correction 1 or 2
WEAK–Re-used cuvette or
inappropriate solution
Possible error
Inappropriate cuvette or
Nucleofector™ Solution or
inappropriate volume in the
cuvette or device possibly
defective.
Re-used cuvette or inappropriate
Nucleofector™ Solution.
Arcing is a complete or partial discharge circumventing the sample
and is often accompanied by a flash and a noise. This problem
is usually caused by imperfect cuvettes or cuvette filling. The
Nucleofector™ II Device is equipped with a hardware safety that
immediately detects arc formation at its beginning to protect the
cells from damage. After the arc interruption the Nucleofector™ II
Device resumes program execution. Normally, the program can
be completed successfully ("arc discharge correction 1 or 2") and
only limited differences in transfection efficiency can be observed.
When repeated arc discharges within one program occurs, it might
be impossible for the Nucleofector™ II Device to complete program
execution. In this case ("Err8 - Arc discharge") significant impacts
on transfection efficiency might be detected. Discard the possibly
damaged cuvette and its contents, reset the device by pressing
the "X" button (1), and repeat the experiment with a new cuvette.
It is not necessary to switch off the Nucleofector™ II Device.
What happened?
Device possibly generated an
arc discharge. Program was
automatically resumed and
successfully completed. A limited
impairment on efficiency and
vitality has to be presumed.
A weak pulse occurred. Only
sub-optimal efficiency might be
achieved.
Procedure
Reset device by pressing any
button. Utilize sample if maximum
efficiency is not essential or try
again with a new sample. For
further samples check if the
volume in the cuvette is 100 µl.
Acknowledge the error message
by pressing any button; utilize
the sample if maximum efficiency
is not essential. Otherwise, try a
new sample.