ChemoMetec NucleoCounter NC-200 User Manual
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ChemoMetec NucleoCounter NC-200 User Manual

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®
™
NucleoCounter
NC-200
Instrument User's Guide
P/N 991-0203
Revision 1.3
拓澳生物：www.topbiox.com
邮箱：sales@topbiox.com

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Summary of Contents for ChemoMetec NucleoCounter NC-200

  • Page 1 ® ™ NucleoCounter NC-200 Instrument User's Guide P/N 991-0203 Revision 1.3 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...
  • Page 2 This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...
  • Page 3 P / N 9 9 1 - 0 2 0 3 ( E n g l i s h ) R e v i s i o n 1 . 3 M a y , 2 0 1 4 ChemoMetec A/S Gydevang 43, DK-3450 Allerod, Denmark Telephone: (+45) 48 13 10 20, Fax: (+45) 48 13 10 21 Internet: www.chemometec.com,...
  • Page 4 This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...
  • Page 5 The material in this document and referred documents is for information only and is subject to change without notice. While reasonable efforts have been made in preparation of these documents to assure their accuracy, ChemoMetec A/S assumes no liability resulting from errors or omi ssions in these documents, or from the use of the information contained herein.
  • Page 6 This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 7: Declaration Of Conformity

    Date: 2011-July-1 Signed: Name: Sarah R. Rasmussen Position: QA/QC Manager Name and address of manufacturer: ChemoMetec A/S Gydevang 43 DK-3450 Allerod DENMARK www.chemometec.com 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com...
  • Page 8 Declaration of Conformity This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 viii 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 9: Weee Directive Information - Europe Only

    WEEE directive information – Europe only WEEE directive information – Europe only Correct Disposal of This Product (Waste Electrical & Electronic Equipment) - Europe only This marking shown on the product or its literature, indicates that it should not be disposed together with other household wastes at the end of its working life.
  • Page 10 WEEE directive information – Europe only This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 11: Introduction And Intended Use

    NucleoCounter® NC-3000. In normal viability analysis the sample volume is as high as 1.4 µl and the ability to detec t cells in clusters is also available. The NucleoCounter® NC-200™ part number at ChemoMetec A/S is 900-0201. 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com...
  • Page 12 Introduction and intended use This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 13: Warnings And Precautions

    The NucleoCounter® NC-200™ shall only be used with an external power supply purchased at ChemoMetec A/S. The detachable DC power supply cord set and appliance inlet of the external power supply are considered as the disconnecting device.

  • Page 14: Electromagnetic Interference

    Warnings and precautions protection provided by the NucleoCounter® NC-200™ and the external power supply may be impaired. Electromagnetic interference NOTE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation.
  • Page 15 Warnings and precautions 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 16: Table Of Contents

    Table of contents Table of contents DECLARATION OF CONFORMITY ..................VII WEEE DIRECTIVE INFORMATION – EUROPE ONLY .............. IX INTRODUCTION AND INTENDED USE ................XI WARNINGS AND PRECAUTIONS ..................XIII INSPECTION, UNPACKING AND INSTALLATION OF EQUIPMENT ........3 ® NC-200™............3 PERATION OF THE UCLEO OUNTER...
  • Page 17 Table of contents ....................19 OWER ON FAILURE TECHNICAL SPECIFICATIONS ..................21 ® NC-200™ ................21 UCLEO OUNTER ....................21 AMPLE MEDIAS ..................... 22 STANDARDS EQUIPMENT AND ACCESSORIES ................23 APPENDIX A: DESCRIPTION OF CHANGES FROM LATEST REVISION ........25 APPENDIX B: WEEE DIRECTIVE INFORMATION IN MORE EU LANGUAGES ......

  • Page 18: Inspection, Unpacking And Installation Of Equipment

    1 Inspection, Unpacking and Installation of Equipment Upon receiving the order from ChemoMetec A/S, the box or boxes should be carefully inspected for any damage that may have occurred during shipping. Any damage must be reported to the carrier and to ChemoMetec A/S immediately.
  • Page 19 1 Inspection, Unpacking and Installation of Equipment This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 20: Cell Counting With The Nucleocounter® Nc-200

     Viability and Cell Count Assay New protocols will be developed on an on-going basis and the updated list can be seen at the web site: www.chemometec.com The NucleoCounter® NC-200™ instrument The NucleoCounter® NC-200™ is shown in the figure below.

  • Page 21: Fluorescence Microscope

    2 Cell counting with the NucleoCounter® NC-200™ 2.1.1 Fluorescence microscope The NucleoCounter® NC-200™ is a fluorescent microscope with a magnification of approximately x1.3. It is equipped with 3 light sources (2 for fluorescent use and one for dark-field analysis). It has a dual-band emission filter. At present only Via1-Cassette is used the NucleoCounter®...

  • Page 22: The Cassette

    2 Cell counting with the NucleoCounter® NC-200™ The Cassette Figure 2 The Via1-Cassette. Here fluorescent dyes AO (Acridine Orange) and DAPI (4',6- diamidino-2-phenylindole) are immobilized in the first part of the flow system. 2.2.1 Loading the Cassette The cassette is loaded by gently pressing the white piston down to the top of the cassette, which creates a partial vacuum in the flow system.

  • Page 23: Sample Volume

    2 Cell counting with the NucleoCounter® NC-200™ The cassettes to be used with NucleoCounter® NC-200™ are highly specialized devices, which hold the key to several of the unique features of the NucleoCounter® NC-200™ concept. For instance in the Via1-Cassette the fluorescent dyes AO (Acridine Orange) and DAPI (4',6-diamidino-2-phenylindole) are immobilized inside the flow channels of the disposable cassette.

  • Page 24: Key, Led Indicator And Pc Control

    3 Key, LED indicator and PC control 3 Key, LED indicator and PC control Interactive Controls Figure 4 The front of the NucleoCounter® NC-200™ The figure above shows the front of the NucleoCounter® NC-200™. Two operational areas are present:  Lid with cassette fixture behind ...
  • Page 25 3 Key, LED indicator and PC control This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 26: Start-Up

    4 Start-up 4 Start-up Power on, power off On the rear of the instrument, two plugs and one fuse holder are located. When seen from the rear the USB 2.0 port is to the left and the fuse holder and DC Power Plug is at the right.

  • Page 27: Starting Up

    4 Start-up 4.1.1 Starting up The NucleoCounter® NC-200™ is connected to a PC by inserting the respective plugs of a USB cable into the instrument and the PC. Once the NucleoView NC-200™ program is launched the stepping motor for the piston actuator performs a homing procedure in order to assure correct positioning of the actuator.

  • Page 28: Operation Of The Nucleocounter

    5 Operation of the NucleoCounter® 5 Operation of the NucleoCounter® Ready mode Once the NucleoCounter® NC-200™ and the NucleoView NC-200™ software are ready, analysis can begin. In the NucleoView NC-200™ software, the browse menu can be used to select the protocol to be performed. Once selected, the run button on the front of the instrument (see Figure 4) or the run button in the software can be used to execute the analysis.

  • Page 29: Actuator Movements

    5 Operation of the NucleoCounter® Figure 9. Illustration of how to hold and place the cassette in the fixture 5.2.1 Actuator movements When a cassette is analyzed after Run is pressed, the piston actuator will move the piston rod down through the cylinder of the cassette. Hereby, the sample material is transported to the cassette measurement chamber.

  • Page 30: Nucleoview Nc-200

    5 Operation of the NucleoCounter® 6 NucleoView NC-200™ Figure 10 NucleoView NC-200™ with a typical result after analysis The NucleoView NC-200™ software controls the NucleoCounter® NC-200™ instrument and image analysis of the recorded images. The program is installed on a Windows PC (Windows 7 or 8).
  • Page 31 5 Operation of the NucleoCounter® The events registered as cells can (when enabled in the protocol) be inspected by launching the Protocol Adaption Editor in the Tools menu and selecting to show events included in the count prior to running an analysis. This will launch a plot manager that shows a scatter plot with a gate that encloses the events record ed as cells.

  • Page 32: Maintenance Of Nucleocounter® Nc-200

    If the contamination is present on every image taken , it is most likely that other optical parts are contaminated. In this case contact ChemoMetec A/S. An example of a relatively large contamination is given in Figure 13.

  • Page 33: Spill Of Liquid

    Even though such phenomena are clearly visible on the image it only ra rely affects the results of the NucleoCounter® NC-200™. Since it is possible to damage the optical system while attempting to clean the system, it is recommended that ChemoMetec A/S should be consulted before the user attempts such cleaning.

  • Page 34: Pc- Maintenance, Error Messages And Power-On Failure

    Under certain conditions the NucleoView NC-200™ software will display error messages during operation. Corrective actions can be found in the Help section of the NucleoView NC-200™ software, but if they do not correct the errors, contact ChemoMetec A/S or the local distributor.
  • Page 35 ChemoMetec A/S. If the NucleoCounter® NC-200™ does not turn on after replacement of the fuse, or if the fuse was not broken, please contact ChemoMetec A/S or the local distributor fo r further instructions.

  • Page 36: Technical Specifications

    9 Technical specifications 9 Technical specifications The NucleoCounter® NC-200™ Optics Lens with x1.3 magnification, 1/2.” CMOS Sample device NC-200™ Cassettes, Excitation (nm) 2 LED light sources with peaks at 365 nm, 505 nm and dark field light Emission (nm) A single dual-band emission filter: 410-460 nm and 540-650 nm Sample consumption 60 μl Analyzed volume...

  • Page 37: Eu Standards

    9 Technical specifications EU standards The NucleoCounter® NC-200™ complies with EU standards as follows. Directives Electromagnetic Compatibility (2004/108/EC). EMC Low Voltage Directive (2006/95/EC) LVD Machinery Directive (2006/42/EC) MD Standards EN 61326-1:2006 Electrical equipment for laboratory use EMC: EN 61000-3-2: 2006 Limits for harmonic current emissions EN 61000-3-3: 1995 +A1 +A2: 2005 Limitation of voltage changes, voltage fluctuations and flicker Standards...

  • Page 38: Equipment And Accessories

    10 Equipment and Accessories 10 Equipment and Accessories On www.chemometec.com an updated list of Equipment and Accessories for the NucleoCounter® NC-200™ are available. 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...
  • Page 39 10 Equipment and Accessories This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...

  • Page 40: Appendix A: Description Of Changes From Latest Revision

    10 Equipment and Accessories Appendix A: Description of changes from latest revision This chapter describes changes from latest revision of this User’s guide. New:  Changes:  Front Page Revision 1.3  Page iii Revision 1.3 May 2014  ® Cell counting with the NucleoCounter NC-200™...
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  • Page 42: Appendix B: Weee Directive Information In More Eu Languages

    Appendix B: WEEE directive information in more EU languages Appendix B: WEEE directive information in more EU languages United Correc t Disposa l of This Product Kingdom (Waste Electr ical & Elec tronic Equ ipment) - Europe only This marking show n on the product or its lite rature , indicates that it should not be disposed t ogethe r w ith othe r household wastes at t he e nd of its work ing life.
  • Page 43 Appendix B: WEEE directive information in more EU languages Norway Korrekt avhending av dette produkt (Avfall elektrisk og elektron isk utstyr) - Kun Europa Denne merk ingen som vises på produktet elle r dens dokument asjon, indikere r at de n ikke skal kastes sammen med annet hus holdni ngsavfall ved slutten av sin levet id.
  • Page 44 Appendix B: WEEE directive information in more EU languages Finland Tämän tuotteen turvallinen hävittämin en (elektroniikka ja sähkölaitteet) - Vain Eu rooppa Oheinen me rkintä tuotteessa tai tuotteen ohe ismate ria alissa me rkitsee , että t ätä t uotetta e i tule hävittää kot italousjättee n mukana sen e linkaa ren päätyttyä.
  • Page 45 Appendix B: WEEE directive information in more EU languages This page was intentionally left blank 991-0203 Rev. 1.3 May 2014 拓澳生物：www.topbiox.com 邮箱：sales@topbiox.com...
  • Page 46 NucleoCounter or NucleoCassette™ shall be construed to imply any warranty or guarantee. ChemoMetec A/S and affiliated companies shall not be held liable for damages and customers shall indemnify ChemoMetec A/S and affiliated companies against liability flowing from use of potentially inaccurate data generated by ...

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