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Summary of Contents for Invitrogen EVOS XL
- Page 1 EVOS XL Core Imaging System ™ For transmitted light applications Catalog Number AMEX1000 Publication Number MAN0007989 Revision B.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 Information in this document is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
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Page 3: Table Of Contents
Contents About this guide .............................. 5 1. Product information ....................6 Product description ............................6 Standard items included ..........................6 Instrument exterior components and manual controls ................7 Descriptions of manual controls ........................8 Onscreen controls ............................9 Descriptions of onscreen controls ....................... 10 2. - Page 4 Appendix D: Safety ...................... 26 Safety conventions used in this document ....................27 Symbols on instruments ..........................28 Safety labels on instruments ........................30 General instrument safety ..........................31 Chemical safety .............................. 32 Chemical waste safety........................... 33 Electrical safety .............................. 34 Physical hazard safety ..........................
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Page 5: About This Guide
About this guide Audience This user guide is for laboratory staff operating, maintaining, and analyzing data using the EVOS ™ XL Core Imaging System. User attention Two user attention words appear in Thermo Fisher Scientific user documentation. Each word implies a particular level of observation or action as described below. words Note: Provides information that may be of interest or help but is not critical to the use of the product. -
Page 6: Product Information
1. Product information Product description EVOS XL Imaging The Invitrogen EVOS XL Core Imaging System (Cat. No. AMEX1000) is a fully ™ ™ ™ automated, digital, inverted imaging system. The system is designed for a broad System range of transmitted light applications including time-lapse imaging, manual- assist cell counting, and image review. -
Page 7: Instrument Exterior Components And Manual Controls
Instrument exterior components and manual controls Phase turret Coarse focus knob Fixed stage with glass stage plate Fine focus knob Freeze button Power switch Save button Power input jack Illumination wheel USB ports Objective turret Mechanical stage The attachable mechanical stage is an optional feature. See “Appendix C: Install the attachable mechanical stage”... -
Page 8: Descriptions Of Manual Controls
Descriptions of manual controls This glossary is not alphabetized. Manual controls are listed in the order they are normally used, with a separate section for the optional Attachable Mechanical Stage (page 25). Power input jack Plug the power adapter into the power input jack. Power switch Set the power switch to “... -
Page 9: Onscreen Controls
Onscreen controls Note: Roll over the bottom of screen with the mouse to activate the onscreen controls. Onscreen controls are only available when a mouse is installed. Login button Scalebar Illumination slider Settings button Freeze/Live toggle Onscreen clock Save image button... -
Page 10: Descriptions Of Onscreen Controls
Descriptions of onscreen controls This glossary is not alphabetized. Onscreen items are listed from left to right. Illumination slider The illumination slider controls the illumination intensity when the mouse is installed. To turn off illumination, slide all the way to the left. Freeze/Live button The Freeze/Live button toggles between Freeze and Live settings. -
Page 11: Installation
2. Installation Operating environment • Place the instrument on a level surface away from vibrations from other pieces of equipment. • Allow at least 5 cm (2 in) free space at the back of the LCD monitor housing to allow for proper ventilation and prevent overheating of electronic components. •... -
Page 12: Connect Power Supply
Connect power supply Connect power Turn the power switch to the “” (OFF) position before connecting the adapter power adapter. Connect the power adapter to the power jack on the back of the instrument base and plug the cord into an outlet. IMPORTANT! Always use the correct power supply. -
Page 13: Optional: Install Attachable Mechanical Stage
Optional : Install attachable mechanical stage Follow the procedure in “Appendix C: Install the attachable mechanical stage” (page 24) to install the mechanical stage. Install the glass stage plate Unpack the glass stage plate and insert it into the stage opening (if a mechanical stage is installed, move it all the way back to expose the stage opening). -
Page 14: Install The Evos ™ Xl Core Imaging System In A Cell Culture Hood
Install the EVOS XL Core Imaging System in a cell culture hood ™ The small footprint of the EVOS ™ XL Core Imaging System, its simple power connection, and its easily-viewed display make it quick to install and convenient to use in a cell culture hood. Dimensions The EVOS ™... -
Page 15: Operation
3. Operation Basic operation The EVOS XL Core Imaging System has both manual and onscreen operation ™ controls. Manual controls include the illumination wheel, focus knobs, freeze and save buttons, objective turret, and the mechanical stage X-Y axis knobs. Onscreen controls appear at the bottom of the screen when the mouse rolls over the controls area (page 9). - Page 16 Set the objective turret to the desired magnification. Turn the phase turret to the position that corresponds to the selected objective and contrast method. Bring the sample into focus using the coarse and fine-focus knobs. Adjust the illumination intensity if necessary, using the illumination wheel or the onscreen illumination slider Use the manual or the onscreen Freeze/Live toggle...
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Page 17: Adjust Instrument Settings
Adjust instrument settings The Settings window provides various controls to customize display performance and image file names and formats. The Settings button toggles to display or hide the Settings window. Note: If the power is switched off before saving the settings, the system reverts to the most recently saved settings. -
Page 18: Update Software
Update software Periodically, Thermo Fisher Scientific adds functionality and other improvements to the EVOS ™ user interface. We recommend keeping your EVOS ™ XL Core Imaging System up to date with the latest software. If you have any questions about software updates, contact your local EVOS distributor. -
Page 19: Care And Maintenance
4. Care and maintenance General care • When cleaning optical elements, use only optical-grade materials to avoid scratching soft lens coatings. • Use the appropriate cleaning solutions for each component, as indicated in the “Decontamination procedures” (page 20). • If liquid spills on the instrument, turn off the power immediately and wipe dry. -
Page 20: Decontamination Procedures
Decontamination procedures To decontaminate the EVOS XL Core Imaging System, follow these procedures: ™ Turn power OFF. Clean the LCD display. Use a soft, dry, lint-free cloth to wipe off any dust from the screen. b. Clean the LCD display with a non-alcohol based cleaner made for flat- panel displays. -
Page 21: Appendix A: Troubleshooting
Appendix A: Troubleshooting Image issues Problem Possible solutions • Set the phase turret to the BF position. Image is too dim, even at brightest • If a condenser slider from a different EVOS ™ instrument has setting (particularly at higher been inserted into the condenser, remove the slider. -
Page 22: Mechanical Issues
Mechanical issues Problem Possible Solutions Entire mechanical stage shifts loosely Tighten screws under stage. See “Appendix B: Install the over fixed stage attachable mechanical stage” (page 24). Use the correct vessel holder for the application (refer to the Vessel does not sit securely when EVOS ™... -
Page 23: Appendix B: Specifications
Appendix B: Specifications EVOS XL Core Imaging System ™ Model (Cat. No. AMEX1100) Infinity-corrected optical system; RMS-threaded objectives with 45 mm parfocal Optics distance 4× / 10× / 20×; Plan LWD Phase, infinity Objectives optional 2× (no phase) & 40× Ph available Objective turret 4-position;... -
Page 24: Appendix C: Install The Attachable Mechanical Stage
Appendix C: Install the attachable mechanical stage The mechanical stage is an optional accessory. You may purchase it separately for use with the EVOS ™ XL Core Imaging System. Go to the product page thermofisher.com/evosxlcore or Technical Support for more information (page 37). Installation/ Follow this procedure to attach the mechanical stage to the fixed... - Page 25 Stage clip Use the stage clip to secure vessel holders or large samples (i.e., a T-75 flask or multi-well vessel) to the mechanical stage. When properly secured, the sample moves with the stage as you turn the X-axis and Y-axis knobs. Pull the stage clip open and place the vessel holder or sample in the back right corner of the mechanical stage opening.
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Page 26: Appendix D: Safety
Appendix D: Safety This section includes the following topics: • Safety conventions used in this document • Symbols on instruments • Safety labels on instruments • General instrument safety • Chemical safety • Chemical waste safety • Electrical safety • Physical hazard safety •... -
Page 27: Safety Conventions Used In This Document
Safety conventions used in this document Safety alert words Four safety alert words appear in Thermo Fisher Scientific user documentation at points in the document where you need to be aware of relevant hazards. Each alert word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular level of observation or action: Definitions IMPORTANT! Provides information that is necessary for proper... -
Page 28: Symbols On Instruments
Symbols on instruments Electrical symbols The following table describes the electrical symbols that may be displayed on Thermo Fisher Scientific instruments. on instruments Symbol Description Indicates the On position of the main power switch. Indicates the Off position of the main power switch. Indicates a standby switch by which the instrument is switched on to the Standby condition. - Page 29 Safety symbols The following table describes the safety symbols that may be displayed on Thermo Fisher Scientific instruments. Each symbol may appear by itself or in combination with text that explains the relevant hazard (see “Safety labels on instruments” on page 30). These safety symbols may also appear next to DANGERS, WARNINGS, and CAUTIONS that occur in the text of this and other product-support documents.
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Page 30: Safety Labels On Instruments
Safety labels on instruments The following CAUTION, WARNING, and DANGER statements may be displayed on Thermo Fisher Scientific instruments in combination with the safety symbols described in the preceding section. Hazard English Français symbol CAUTION! Hazardous chemicals. Read the ATTENTION! Produits chimiques Safety Data Sheets (SDSs) before handling. -
Page 31: General Instrument Safety
General instrument safety WARNING! PHYSICAL INJURY HAZARD. Use this product only as specified in this document. Using this instrument in a manner not specified by Thermo Fisher Scientific may result in personal injury or damage to the instrument. Moving and lifting CAUTION! PHYSICAL INJURY HAZARD The instrument is to be the instrument moved and positioned only by the personnel or vendor specified in the... -
Page 32: Chemical Safety
Chemical safety Chemical hazard WARNING! CHEMICAL HAZARD. Before handling any chemicals, refer warning to the Safety Data Sheet (SDS) provided by the manufacturer, and observe all relevant precautions. WARNING! CHEMICAL HAZARD. All chemicals in the instrument, including liquid in the lines, are potentially hazardous. Always determine what chemicals have been used in the instrument before changing reagents or instrument components. -
Page 33: Chemical Waste Safety
Chemical waste safety Chemical waste CAUTION! HAZARDOUS WASTE. Refer to Safety Data Sheets (SDSs) hazard and local regulations for handling and disposal. Chemical waste To minimize the hazards of chemical waste: safety guidelines • Read and understand the Safety Data Sheets (SDSs) provided by the manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste. -
Page 34: Electrical Safety
Electrical safety DANGER! ELECTRICAL SHOCK HAZARD. Severe electrical shock can result from operating the EVOS ™ XL Core Imaging System without its instrument panels in place. Do not remove instrument panels. High‐ voltage contacts are exposed when instrument panels are removed from the instrument. -
Page 35: Physical Hazard Safety
Physical hazard safety Moving parts WARNING! PHYSICAL INJURY HAZARD. Moving parts can crush and cut. Keep hands clear of moving parts while operating the instrument. Disconnect power before servicing the instrument. Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, and blood of humans and other animals have the potential to transmit infectious diseases. -
Page 36: Safety And Electromagnetic Compatibility (Emc) Standards
Safety and Electromagnetic Compatibility (EMC) Standards This section provides information on: • U.S. and Canadian safety standards • European safety and EMC standards • Australian EMC standards U.S. and Canadian The CSA C/US Mark signifies that the product meets applicable U.S. and Canadian standards, including those from CSA, CSA America, ANSI, ASME, Safety Standards ASSE, ASTM, NSF and UL. -
Page 37: Documentation And Support
Documentation and support Obtaining support Technical support For the latest services and support information for all locations, visit www.thermofisher.com. At the website, you can: • Access worldwide telephone and fax numbers to contact Technical Support and Sales facilities • Search through frequently asked questions (FAQs) •... - Page 38 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 16 October 2017...
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