Thermo Scientific Orbitrap Fusion Lumos MS Getting Connected Manual
Thermo Scientific Orbitrap Fusion Lumos MS Getting Connected Manual

Thermo Scientific Orbitrap Fusion Lumos MS Getting Connected Manual

Orbitrap fusion series

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Orbitrap Fusion Series
Getting Connected Guide
80011-97002 Revision C
June 2017

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  • Page 1 Orbitrap Fusion Series Getting Connected Guide 80011-97002 Revision C June 2017...
  • Page 2 © 2017 Thermo Fisher Scientific Inc. All rights reserved. EASY-Max NG, Fusion, Ion Max NG, and Lumos are trademarks, and Orbitrap Fusion, Orbitrap Fusion Lumos, Thermo Scientific, Tribrid, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States.
  • Page 3 Thermo Fisher Scientific. To ensure continued compliance with the regulatory standards, order replacement parts and additional components, options, and peripherals directly from Thermo Fisher Scientific or an authorized representative. Regulatory compliance results for the following Thermo Scientific™ products: • Orbitrap Fusion Lumos MS •...
  • Page 4 Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations: This instrument must be used in the manner specified by Thermo Fisher Scientific to ensure protections provided by the instrument are not impaired.
  • Page 5 WEEE Directive 2012/19/EU Thermo Fisher Scientific is registered with B2B Compliance (B2Bcompliance.org.uk) in the UK and with the European Recycling Platform (ERP-recycling.org) in all other countries of the European Union and in Norway. If this product is located in Europe and you want to participate in the Thermo Fisher Scientific Business-to-Business (B2B) Recycling Program, send an email request to weee.recycle@thermofisher.com with the following information:...
  • Page 7: Table Of Contents

    Index ..............27 Thermo Scientific...
  • Page 8 Contents viii Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 9: Preface

    Use the information in this guide to install the MS system in your laboratory and connect any external liquid chromatography (LC) devices. External devices include those that are controlled from or are independent of Thermo Scientific mass spectrometry applications, such as the Thermo Xcalibur™ data system.
  • Page 10: Accessing Documentation

    Note Highlights information of general interest. Highlights helpful information that can make a task easier. Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 11 Risk of eye injury: Eye injury can occur from splattered chemicals, airborne particles, or sharp objects. Wear safety glasses when handling chemicals or servicing the instrument. Trip obstacle: Be aware of cords, hoses, or other objects located on the floor. Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 12: Contacting Us

    Note To provide feedback for this document, go to surveymonkey.com/s/PQM6P62 or send an email message to Technical Publications (techpubs-lcms@thermofisher.com). You can use your smartphone to scan a QR Code, which opens your email application or browser. Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 13: Vacuum System

    IMPORTANT For optimal performance of the forepumps, place them as follows: • For the Orbitrap Fusion Lumos MS, place the forepumps under the workbench. • For the Orbitrap Fusion MS, place the forepump under the workbench or within 2.4 m (8 ft) of the instrument.
  • Page 14: Chapter 1 Vacuum System

    Vacuum System Connecting the Forepumps to the Mass Spectrometer  To attach the vacuum hose assembly Orbitrap Fusion Lumos MS Orbitrap Fusion MS Using a centering ring and a swing clamp, connect a 90-degree plumbing elbow to the MS’s back...
  • Page 15: Connecting The Forepumps To The Lab Exhaust System

     To connect the forepumps to the exhaust system (Orbitrap Fusion Lumos MS) If you removed the workbench’s front panel (as shown), reattach it. Connect one end of the exhaust tubing to the forepump exhaust ports.
  • Page 16 Vacuum System Connecting the Forepumps to the Lab Exhaust System Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 17: Gas Connections

    CAUTION You must use either copper (provided) or stainless steel for the UHP gas lines. CAUTION After completing these connections, route the tubing so that they are not a trip hazard. Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 18: Chapter 2 Gas Connections

    Gas Connections  To connect the gas lines to the back of the MS (Orbitrap Fusion Lumos MS) (HP nitrogen gas) Cut a length of the Teflon™ PFA tubing and push it into the Nitrogen In inlet. On the other end, connect an appropriate fitting that connects to your HP nitrogen gas supply.
  • Page 19: Api Source And Waste Container Connections

    API Source and Waste Container Connections To install the Thermo Scientific EASY-Max NG™ API source and the solvent waste container to the Orbitrap Fusion Series MS, follow these procedures. Contents • Installing the API Source • Connecting the Solvent Waste Container Installing the API Source All the wiring and gas plumbing for the API source are internal.
  • Page 20: Connecting The Solvent Waste Container

    Vent the waste container to a dedicated fume exhaust system. The exhaust system for the API source must accommodate a flow rate of up to 30 L/min (64 ft /h). Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 21  To connect the solvent waste container to the MS Connect the tubing to the MS’s Drain/Waste port and (Orbitrap Fusion Lumos MS) the external vent system. Connect the filling/venting cap to the solvent waste container Then, connect the Tygon waste tubing (1 in.
  • Page 22 API Source and Waste Container Connections Connecting the Solvent Waste Container Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 23: Syringe Pump And Modular Valve Assembly

    Place the valve on the bottom shelf; using the (Not shown) Place the assembly on the top, left screwdriver and two screws, attach the top shelf. surface of the MS and adjust its position as needed. Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 24: Chapter 4 Syringe Pump And Modular Valve Assembly

    Syringe Pump and Modular Valve Assembly Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 25: Chapter 5 Electrical Connections

    CAUTION Safety and EMC regulations require the use of Category 5e, shielded Ethernet communication cables, maximum 3 m (10 ft) long. CAUTION After completing these connections, route all cables and power cords so that they are not a trip hazard. Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 26 Tip For troubleshooting purposes, you might want to record which devices connect to which ports on the Ethernet switch. The back of the data system computer might not be as shown. Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 27 The locking levers have three positions. To fully unlock the roll and swivel movements, push the levers back to the third position (almost horizontal). 3. Reattach the front panel when you are ready. Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 28 Electrical Connections Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 29: Chapter 6 External Devices

    Contact Closure Signal The Orbitrap Fusion Series MS can start data acquisition upon receiving a contact closure (start) signal from an external LC device, which is typically an autosampler. Thermo Scientific mass spectrometry applications can control external devices (for example, autosamplers, pumps, and detectors) from several manufacturers including Thermo Fisher Scientific, Agilent Technologies, and Waters Corporation.
  • Page 30: Communication Connectors

    Peripheral Control Ground Earth ground. Provides a 5 Vdc, 500 mA output (with pin 1). Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 31 10V Max: (Channel 1) Provides a connection for an external + (positive, pin 7) and device, such as an LC instrument. – (negative, pin 8) Input: 0–10 Vdc (voltage clamps at 15 Vdc) Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 32 Ethernet 1000 Base T Provides a connection for the Ethernet switch. Figure 1. Communication connectors (left side of the MS) Reset button Peripheral Control connector Analog Input connector USB ports Ethernet port Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 33: Appendix A Installation Kits And Power Cords

    Chemyx™ Fusion 100T syringe pump with a power cord 00109-99-00045 and USB interface cable Rheodyne™ MX Series II™ modular divert/inject valve 00109-99-00046 (six-port, two-position) with an ac adapter, power cord, USB interface cable, and high-pressure fitting sets Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 34: Single Mechanical Pump Kit

    NW40 Swing clamp, aluminum, NW32/40 00108-02-00004 Exhaust waste connection Tubing, Tygon, 3/4 in. (19.1 mm) OD, 0.5 in. 00301-22920 (12.7 mm) ID, 3 m (10 ft) long Only one piece is required. Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 35: Dual Mechanical Pumps Kit

    Exhaust waste connection Fitting Tee, barbed, nylon, for 0.5 in. (12.7 mm) ID 00103-01-00012 tubing Tubing, Tygon, 3/4 in. (19.1 mm) OD, 0.5 in. 00301-22920 (12.7 mm) ID, 6 m (20 ft) long Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 36: Ms Setup Kit

    (1.57 mm) thick, 4.6 m (15 ft) long (for the HP nitrogen gas) – Fittings to connect the copper and PFA tubings to the – – gas supplies (customer provided) Solvent waste connection Container, Nalgene™, 4 L heavy-duty; filling/venting cap 80100-20265 Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 37: Power Cords

    Part numbers are provided for Thermo Fisher Scientific field service use only. “International” designation is for countries or territories that do not use the North American plug configuration. Not specified in this guide Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 38 Installation Kits and Power Cords Power Cords Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...
  • Page 39: Index

    See contact closure, start instrument signal forepump connections symbols, meaning fume exhaust system cautions vacuum hose forepumps fume exhaust system cautions Thermo Fisher Scientific website, user documents gas ballast valve, opening tools maintenance instructions troubleshooting, Ethernet switch Thermo Scientific Orbitrap Fusion Series Getting Connected Guide...
  • Page 40 Index: V valve, LED viewing documents WEEE directive Orbitrap Fusion Series Getting Connected Guide Thermo Scientific...

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