Page 1 Thermo Scientific SkanIt Software for ® Varioskan ® version 2.4.3 User Manual Rev. 3.1...
Page 3 Thermo Scientific ® SkanIt Software for ® Varioskan version 2.4.3 User Manual Rev. 3.1...
Page 4 Copyright © 2007 Thermo Fisher Scientific. All rights reserved. First edition printed in 2004. Printed in Finland. Reproduction of the accompanying user documentation in whole or in part is prohibited. Trademarks SkanIt”, Varioskan” and Microtiter” are registered trademarks of Thermo Fisher Scientific. All other trademarks and registered trademarks are the property of their respective holders. Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments.
Page 5: Table Of Contents
Appendix A Administrators ................... 275 Appendix B Database maintenance ..............285 Appendix C Drug Discovery Edition with 21CFR11 Compliance ......293 Glossary ..................... 303 Index ....................307 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 7 For more information ................15 Warning and other markings used in the documentation ....16 Chapter 1 Introduction ..................17 SkanIt Software ................... 17 Varioskan system ................. 18 Chapter 2 Installing SkanIt Software ..............19 Before installation ................19 Registering SkanIt Software ............... 19 Checking the PC requirements ............
Page 8 Using multipoint in a measurement ........... 114 Kinetic measurements ................ 116 Incubate .................... 116 Shake ....................117 Shaking speed and diameter combinations ........119 Dispense .................... 120 Pause ....................122 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 9 HBW calculation ................178 Ratio within spectrum ..............181 Ratio between spectra ..............182 Select wavelengths ................183 Kinetic calculations ................183 Average rate ..................184 Maximum rate ................185 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 10 Dispensers ..................234 Reports .................... 236 Colors ....................237 Plate template settings ............... 238 Creating plate templates ..............239 Modifying plate templates ............... 240 Plate tray ................... 242 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 11 Unlocking user accounts ..............278 Groups ....................279 General settings ................. 282 Company settings ................282 Appendix B Database maintenance ..............285 Backup ....................286 Restore ....................287 Archive ....................288 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 12 Signing a session or a run ..............298 Viewing signatures ................299 Date and time format ................ 300 Audit trail ..................300 Glossary ..................... 303 Index ....................307 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 13 Figure 1-1 Operation principle ............18 Figure 1-2 Varioskan spectral scanning multimode reader ....18 Figure 2-1 Computer and mains power supply connectors in the Varioskan instrument ..............38 Figure 3-1 Different parts of the main window ........42 Figure 3-2 Main window for protocol editing ........
Page 14 Determining the average value of a kinetic measurement .. 196 Figure 10-17 Determining the baseline subtracted kinetic curve ... 197 Figure 14-1 Menus and action panels of SkanIt Software ....259 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 15 Table 13-1 Available Settings menu items for different security groups ................. 225 Table 13-2 Number formats ............230 Table A-1 Rights of the different security groups ......280 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 16 Tables ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 17: About The User Manual
About the User Manual Intended users The user manual has been written for the users of the Thermo Scientific Varioskan spectral scanning multimode reader and provides information on Thermo Scientific SkanIt Software for Thermo Scientific Varioskan. The manual contains the operating instructions for Thermo Scientific SkanIt Software version 2.4.3 Research Edition (RE) and Drug Discovery Edition...
Page 18: Warning And Other Markings Used In The Documentation
Note Marks a tip, important information that is useful in the optimum operation of the system, or an item of interest. Tip Gives a helpful hint for getting the most out of the software functionality. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 19: Chapter 1 Introduction
Chapter 1 Introduction SkanIt Software SkanIt Software is used to control the Varioskan spectral scanning multimode reader. SkanIt Software provides features needed to define and run a variety of assays and to make comprehensive calculations and reports. A plate template database covers the most common plate formats.
Page 20: Varioskan System
Varioskan system Figure 1-1. Operation principle Varioskan system The Thermo Scientific Varioskan (Figure 1-2) is an advanced spectral scanning multimode reader. The Varioskan is used to measure fluorescence intensity, time-resolved fluorescence (TRF), and absorbance in end point, kinetic and spectral measurements in the UV/Vis/NIR range from appropriate microplate formats.
Page 21: Chapter 2 Installing Skanit Software
Pointing device Mouse or equivalent is necessary CD-ROM drive Monitor / color settings XVGA monitor with 1024 x 768 resolution Browser Microsoft Internet Explorer 6.0 (or greater) installed ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 22: Microsoft Windows Settings
The power options settings can be checked and changed in the Power Options Properties window: ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 23: Letters Permitted With Skanit Software
Insert the SkanIt Software installation CD into the CD-ROM drive of your PC. The SkanIt Software Setup window appears automatically. If it does not, launch the installation from the CD; double-click the Setup.exe file. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 24 Select Yes, if you do not know the system administration (sa) password of the existing database engine. The installation steps are shown. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 25: Installing Software Prerequisites
You can also use the advanced installation option to update the instrument's embedded software. Proceed with updating the embedded software as instructed in a separate document Upgrading Thermo Scientific Varioskan to SkanIt Software 2.4.3. Installing software To install software prerequisites, follow these steps:...
Page 26 Step 7 and also required for maintaining the database. • Confirm SA Password - Retype the password to confirm the new password. Click OK. The MSDE is installed. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 27 PC or that it is turned off before you continue with the USB Driver installation. Click Yes. The Varioskan Flash USB Driver Setup is launched. Click Next. You have to first accept the End User License Agreement. Select I accept this EULA and click Next.
Page 28: Installing The Software
However, the setup will inform you about it and the setup will continue after the restart. Installing the software When the prerequisites have been installed, the SkanIt for Varioskan Setup will proceed with the actual SkanIt Software installation. ®...
Page 29 The Enter Serial Number And Installation Code dialog is shown. You have to first enter SkanIt Software licensing and customer information. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 30 It is recommended to use these suggestions. Change them only if absolutely necessary. The recommended free disk space is minimum 2.5 GB for the SkanIt Software database. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 31 The Setup Wizard is now ready for the actual installation. Click Next to proceed. The installation files are copied to the selected folder. The Database Configuration is performed next. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 32 Click Configure. The installation proceeds with the database configuration. When the installation is complete, you will receive a notification of it. Click Close. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 33 Start > Programs > Thermo SkanIt Software > SkanIt RE/DDE for Varioskan 2.4.3. Or: double-click the Skanit for Varioskan shortcut icon on your desktop. The Log On To SkanIt Software dialog is shown. 10. When logging in for the first time, enter admin” in the User name field and leave the Password field empty.
Page 34: Installing Dde And Re Versions To One Computer
PC to operate several instruments, such as models Varioskan Flash, Multiskan Spectrum and Appliskan, you need to install separate versions of SkanIt Software onto your PC. The installation procedure is similar for all versions.
Page 35: Uninstalling Skanit Software
Reinstalling the software The reinstallation mode is not supported. You have to uninstall SkanIt Software and then install a fresh copy. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 36: Communicating With Instruments
54), if the Connect to default instrument at startup setting is selected (see General” on page 226). • Setup... - You can edit an existing instrument (see below). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 37 Close - Close the dialog. If your instrument is not included in the list, add the instrument to the database: select Varioskan on COM1 and click Duplicate. The Save Instrument As... dialog opens. Type the name of your instrument in the field and click OK.
Page 38 You can now change the default instrument to be the newly added instrument. Select the new instrument from the Instruments list and click Set Default. Note You must disconnect from the current default instrument first. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 39: Connecting The Instrument To The Pc Through A Com Port
The new instruments are available immediately in the Instruments list and in the list in the Execution action panel. Connecting the instrument The PC and the Varioskan instrument are connected by using an RS-232 to the PC through a COM C serial cable.
Page 40: Connecting The Instrument To The Pc Through A Usb Port
Installing SkanIt Software Communicating with instruments Figure 2-1. Computer and mains power supply connectors in the Varioskan instrument Switch ON the instrument and then the PC. Launch SkanIt Software as described in Launching SkanIt Software” on page 54. After startup the instrument is ready for operation. Since the instrument calibrates itself, you can start measuring immediately as soon as the instrument has been turned on.
Page 41 Communicating with instruments Select No, not this time and click Next. Make sure the installation CD is in the CD drive. Select Install the software automatically and click Next. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 42 Installing SkanIt Software Communicating with instruments Select the appropriate choice from the list and click Next. Click Finish. The instrument is now ready for operation. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 43: Chapter 3 User Interface
Chapter 3 User Interface Navigating in the When a session is open, the main window of SkanIt Software for Varioskan is divided into six areas (see Figure 3-1): software • The Session action panel on the upper left (1) has buttons to create, open and save sessions and to view the Help.
Page 44: Protocol Editing Window
Protocol is selected from the session list, and the desired step is added to or selected from the Steps tree. The parameters of the selected step can now be viewed or edited in the main panel. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 45: Plate Layout Editing Window
When a well is selected, its properties can be viewed or edited in the Sample Properties panel. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 46: Figure 3-3 Main Window For Plate Layout Editing
2 - Tab view of sample properties • 3 - Main panel showing the plate layout and the well definitions For more information on editing a plate layout, see Chapter 8: Plate Layouts”. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 47: Results Viewing Window
1 - Report toolbar • 2 - Tree view of measurements and their associated calculations • 3 - Main panel showing the status report of the selected session ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 48: Figure 3-5 Main Window For Results Viewing - Measurement Results As A
2 - The main panel showing the results of the selected measurement in the plate layout format For more information on viewing the measurement results, see Calculation results” on page 206. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 49: Figure 3-6 Main Window For Results Editing - Calculation Parameters
Navigating in the software Figure 3-6. Main window for results editing - calculation parameters Figure 3-7. Main window for results viewing - calculation results as a list ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 50: Run Log Viewing Window
Figure 3-8. Main window for run log viewing • 1 - Run status information • 2 - Main panel showing the log of the executed session ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 51: Exploiting The Software Features Fully
User Interface Exploiting the software features fully Exploiting the software You can speed up your use of SkanIt Software for Varioskan by using the following features. features fully Lists Click the list title bar to sort the entries in alphabetical order. The order can be ascending or descending.
Page 52: Right-Click Menus
You can use the arrow keys to select radio buttons, move sliders and change the ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 53: Shortcuts
Connect to the default instrument Disconnect from the default instrument Tooltips Tooltips appear when you move the cursor over a button in a toolbar or an action panel. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 54 Move the cursor over the icon to view instructions. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 55: Chapter 4 Sessions
The general operational procedure with SkanIt Software is as follows: procedure • Launch the SkanIt Software application. • Load an existing session or create a new one. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 56: Launching Skanit Software
Software Start > Programs > Thermo SkanIt Software > SkanIt RE/DDE for Varioskan 2.4.3. Or: double-click the SkanIt RE/DDE for Varioskan 2.4.3 shortcut icon on your desktop. The Log On To SkanIt Software dialog appears. Enter your user name and password and click OK. The software starts to load.
Page 57: Managing Folders In The Tree View
Creating new folders You can create new folders in the tree view when you: • create a new session with Session > New in the Definitions done dialog. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 58 Right-click on the parent folder and select New Folder from the pop-up menu. A new folder is created. Give a name to the new folder (see also Renaming folders” on page 57). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 59: Moving Folders Or Sessions
Session > Open in the Open Existing Session/Run dialog. To rename a folder: Select one of the steps above. Select the folder you want to rename. Click Rename. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 60: Deleting Folders
If there are more than one session in the folder, you cannot delete it. To delete a folder: Select one of the steps above. Select a folder you want to delete. Click Delete. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 61: Opening An Existing Session
You can create new folders, rename folders, move sessions between folders and delete folders, sessions or runs. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 62 If you only open the session of an executed run, only the session information will be opened and the measurement data will not be available. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 63: Creating A New Session
247. Also see Using pathlength correction in a measurement” on page 97 and Pathlength correction” on page 72). Give a name to the session in the Session name field. The Next button becomes available. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 64 Use existing - Use a previously created plate layout. The list displays all plate layouts and the user names of the plate layout creators. Select the plate layout from the list. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 65: Editing A Session
You can select an existing folder or create a new one (click New Folder or right-click on the parent tree) in the tree view for storing the session. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 66: Deleting A Session Or A Run From The Database
If the run or session is currently open, you cannot delete it unless you first close it. Close the item and select it again for deletion. Note If you delete a session, all of its runs are also deleted. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 67: Exporting And Importing Sessions
SkanIt Software protocol database. Sessions and runs that have been importing sessions exported from another instance of SkanIt Software for Varioskan 2.4.1 or 2.4.3 can be imported to the database. Exporting a session does not export the measurement results. You must export runs to export the measurement results.
Page 68: Importing Sessions Or Runs
Select the SkanIt data file (*.msd) by browsing in the Open dialog and click Open. The file should be indicated by _VS”. The file path appears in the File location text box. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 69 An imported session is shown in the folder tree with the text [import] in front of it. If the imported session or run has dispenser information, the information is also shown even if the connected instrument has no dispensers installed. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 70 Sessions Exporting and importing sessions ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 71: Chapter 5 Executing A Session
Open the session (see Opening an existing session” on page 59). If the session is already open, make sure that the session has been saved. Insert the plate into the plate tray (see the Thermo Scientific Varioskan User Manual). Caution Make sure you do not dispense into the instrument by mistake.
Page 72 Maximum - Displays the maximum value that has been measured up to the point. • Reading - Shows the ordinal of the execution loop (kinetic loop or well loop). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 73 If you have more than one plate in the plate layout, the software prompts you to insert the subsequent plates into the instrument after measuring the previous plate. When you have inserted the plate, click OK. The measurement starts. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 74: Pathlength Correction
975 nm measurement. See Figure 5-1. This absorption band can be used to calculate the pathlength of the aqueous solution being measured in a microplate and then used to normalize the data. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 75: Measuring New Pathlength Correction K-Factors
Select the correct plate type from the Plate layout options list and click Next. Select a folder where the session is saved. Click Finish. A ready protocol opens in the main window. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 76 975 nm. The calculated coefficient of variation (CV%) of each set of data must be less than 10%. Click the Add to Solvent List button. The Add New Pathlength Correction Factor dialog is shown. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 77: Priming Dispensers
Caution You must always prime into a waste vessel by removing the dispensing head from the dispensing head positioner. Hold on to the dispensing head while priming. Select Execute > Prime... or click the button. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 78: Emptying Dispensers
When the priming is finished, click Close. You can also prime the tubing using the Prime/Empty control switch located on top of the dispenser (see the Thermo Scientific Varioskan User Manual). Emptying dispensers You can empty the dispenser tubing to prevent wasting expensive reagents.
Page 79: Washing Dispensers
For more information, see the instrument manual. Washing dispensers Select Execute > Wash..The Wash Dispenser dialog opens. For more information, see Example of dispenser washing” on page 78. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 80: Example Of Dispenser Washing
Executing a Session Washing dispensers The Wash Dispensers dialog has the following functions: • Select Dispenser(s) - The Varioskan is equipped only with one dispenser. • Washing - Select the washing step and its settings. All the selected dispensers perform the step simultaneously.
Page 81 10. Place the aspirate tubing into distilled water. 11. Flush the dispenser syringe and tubing between 1 to 30 times. Select the required 1 to 30 cycles and click Flush. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 82 Executing a Session Washing dispensers 12. Empty the dispenser syringe and tubing of distilled water. Click Dispose. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 83: Chapter 6 Protocols
Plate template - Select the plate template used. The plate type selected in the Create New Session wizard (see Creating a new session” on page 61) is suggested. You can only change the plate type to another ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 84 The black dot designates the starting point and the arrows show the execution order (Figure 6-1). Use the up and down arrows to switch between the different options. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 85: Adding New Protocol Steps
(see Steps action panel” on page 264) or right-click in the Steps tree and select the step from the menu. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 86 Area1 followed by the Fluorometric1 measurement performed for Area2. The Dispense1 and Shake1 steps are applied next for the entire layout, followed by KineticLoop1, which includes the Dispense2 and Photometric2 steps. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 87: Deleting Protocol Steps
For steps that are not inside any area definition, the operation area consists of all the wells that contain blanks, unknowns, controls and/or calibrators. You can select according to an area or a group. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 88 In Group selection, select the group and/or the sample type(s) by ticking the appropriate check box(es). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 89: Well Loops
Settle delay value. This option is not available when the execution block type is set to Well and the well count is 1 (see below). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 90 The following 24-well plate will be measured with a kinetic dispense and kinetic dispense and fluorometric measurement with dispense in reading 1: fluorometric measurement The following settings are selected: ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 91: Measurement With Dispensing
Figure 6-2 shows a measurement with dispensing. The delay between the start of dispensing and the start of measurement is so small that no liquid has yet reached the well when the ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 92: Figure 6-2 Measurement After Dispense And Measurement Together With
Select at which reading the dispensing will be performed. The possible values are from 1 to the number of readings defined for the kinetic loop, the default being 1. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 93: Kinetic Loops
Readings - Enter the number of times the kinetic loop is executed. The default value is 10. • Interval [hh:mm:ss:t] - Enter the time that elapses between the starting times of two consecutive loops. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 94 Protocols Kinetic loops The Lag time and Step duration parameters are not available in the measurement step. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 95: Chapter 7 Step Parameters
The calibration is valid for 1 hour. Select Steps > Photometric or click the button on the action panel. The photometric measurement step includes the following parameters: ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 96 The calibration is valid for 1 hour. Defining multiple When you select the Multiple Measurements check box while defining photometric measurements the photometric measurement parameters, the following dialog opens: ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 97: Photometric Spectrum Scanning
The bandwidth of 5 nm by means of a slit is used in the measurement. Select Steps > Photometric Scanning or click the button on the action panel. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 98 Measurement time [ms] - The measurement or integration time defines the light collection time used in the measurement. The wells are measured with a selected measurement time. For more information, see Measurement times” on page 113. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 99: Using Pathlength Correction In A Measurement
Note A background subtraction step is automatically added to the pathlength correction calculation. Therefore, blanks are needed in the plate layout. Tick the Pathlength correction check box. The Pathlength Correction K-Factors dialog is launched. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 100 Add the pathlength correction for each measurement step separately. Note If you use a pathlength correction factor which did not pass the quality control check, the measurement results will be unreliable ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 101: Fluorometric Measurement
12 nm. The emission bandwidth is permanently set to 12 nm and cannot be changed. Note To prevent any stray light from the excitation light from interfering with the emission light, the difference between the ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 102 Top - Fluorescence is measured at the measurement position above the plate. • Bottom - This option is available with the Varioskan Flash only. • Multiple measurements - Tick the check box to enter multiple wavelength pair values. All wavelengths are measured one after the other from the same well before moving to the next well.
Page 103: Defining Multiple Fluorometric Measurements
In the Fluorometric Spectrum Scanning step, either the emission or the excitation wavelength is fixed and the other wavelength is scanned. scanning Select Steps > Fluorometric Scanning or click the button on the action panel. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 104 If the 5 nm excitation and 12 nm emission (permanent) bandwidths are used, the difference between excitation and emission wavelengths should be at least 17 nm. With the 12 nm ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 105: Trf Measurement
Top - Fluorescence is measured at the measurement position above the plate. • Bottom - This option is available with the Varioskan Flash only. TRF measurement The TRF Measurement step is used for measuring time-resolved fluorescence using special TRF labels at selected excitation and emission wavelengths.
Page 106 If the multipoint measurement is not selected, only the center of the well is measured. Multipoint is only available for 6 to 48-well plates. For more information, see Using multipoint in a measurement” on page 114. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 107 Top - Fluorescence is measured at the measurement position above the plate. • Bottom - This option is available with the Varioskan Flash only. • Multiple measurements - Tick the check box to enter a multiple wavelength pair and TRF parameter values. For more information, see Defining multiple TRF measurements”...
Page 108: Defining Multiple Trf Measurements
The list shows the current measurement parameters, that is, the excitation (Ex) and emission (Em) wavelengths and the delay time and integration time values. To add new measurements and edit the current values, click Edit..® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 109: Trf Scanning
In the TRF Scanning step, either the emission or the excitation wavelength is fixed and the other wavelength is scanned. Select Steps > TRF Scanning or click the button on the action panel. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 110 (permanent) bandwidths are used, the difference between excitation and emission wavelengths should be at least 17 nm. With the 12 nm excitation and emission bandwidths the difference should be at least 24 nm. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 111: Trf Decay
Top - Fluorescence is measured at the measurement position above the plate. • Bottom - This option is available with the Varioskan Flash only. • TRF delay time [µs] - Define the waiting period (TRF delay) between the excitation flash and the beginning of the emission light measurement (see Figure 7-1).
Page 112 (permanent) bandwidths are used, the difference between excitation and emission wavelengths should be at least 17 nm. With the 12 nm excitation and emission bandwidths the difference should be at least 24 nm. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 113 Stop time [µs] - Define the time from the flash to the middle point of the last mesurement (see Figure 7-2). • Step [µs] - Define the time difference between two sequential middle points of the measurement (see Figure 7-2). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 114: Dynamic Range Selections
Top - Fluorescence is measured at the measurement position above the plate. • Bottom - This option is available with the Varioskan Flash only. Dynamic range AutoRange (default) selects automatically that the optimal reading range is used. It is based upon signal intensity in the well and uses the lowest possible selections reading range to obtain best sensitivity.
Page 115: Measurement Times
100 ms measurement times are recommended to reduce noise if the measured absorbance is too high. The result is the mean value of individual 10 ms readings during the total measurement time. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 116: Using Multipoint In A Measurement
In Figure 7-4, the measured points are orange. Their coordinates are shown as values on the X and Y axes. For example, 190 : 0 means 1.90 mm left in the X direction from the origin. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 117: Figure 7-4 Multipoint Example: Measurement Points In A Well And Their
Horizontal line across well - Selects a horizontal line in the middle of the well. Vertical line across well - Selects a vertical line in the middle of the well. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 118: Kinetic Measurements
Incubation can be carried out with 24 to 1536-well plates. The incubation step includes the following parameters: • Incubation time - The duration of the incubation (hh:mm:ss). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 119: Shake
Shake The Shake step is used for shaking the microplate (orbital shaking) in order to mix the samples. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 120 ON time. If you select Background, the instrument shakes during idle periods. With background shaking the shaking always ends with an ON time. Duration is not available in the background mode. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 121: Shaking Speed And Diameter Combinations
The allowed combinations are those that are accepted by the software but may cause spilling. Only the recommended (dark gray) speed and diameter combinations should be used. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 122: Dispense
Caution Make sure that there is a plate in the plate carrier before dispensing to avoid dispensing into the instrument. Select Steps > Dispense or click the button on the action panel. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 123 The dispensing step includes the following parameters: • Dispenser - Select which dispenser will be used. Only one dispenser is available with the Varioskan instrument. Also see Dispensers” on page 234. • Volume [µl] - Enter the volume to be dispensed into all the wells in the defined area.
Page 124: Pause
The instrument continues to execute the session after the condition set in Continuation options is met. Select Steps > Pause or click the button on the action panel. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 125: Plate In
The Plate out step is used for running the plate out of the instrument. Select Steps > Plate Out or click the button on the action panel. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 126 Step Parameters Plate Out The step has no parameters. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 127: Chapter 8 Plate Layouts
Plate Layout on the Session view to display the plate layout (Figure 8-1). The plate layout is based on the selection made in the Create New Session wizard. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 128: Figure 8-1 Plate Layout Editing Window
Figure 8-1. Plate layout editing window • 1 - Plate layout is selected • 2 - Several plates can be defined • 3 - Samples are added with Fill Wizard ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 129: Editing A Plate Layout
Fill Wizard (see Adding samples to the plate layout using Fill Wizard” on page 128 below), but you can also add samples using the Sample ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 130: Adding Samples To The Plate Layout Using Fill Wizard
You can change the well from which you start filling by clicking it. If you start filling from the right side of the plate, remember to change the Filling order arrows accordingly (see below). Samples: ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 131 • Specific blanks - Select the number of specific blanks (individual sample blanks) in use: none, one or two. If you select one or two specific blanks, ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 132 The file must only contain sample names (no spaces allowed) separated by line breaks (see Example 8-1, Text file for defining names for unknown samples” below). The Load IDs button is only available for Unknowns. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 133: Adding A Concentration Or Dilution Series
When you select Generate series in the Fill Wizard dialog and click Concentrations or Dilutions, the Concentrations or Dilutions dialog is launched. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 134 Editing a plate layout See below for an explanation of the fields. Example 8-2, Adding a concentration series” shows an example of adding automatically a concentration series. Quantities ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 135 If you select Multiple Values, the following parameters are available: • Dilutions/Concentrations Enter each concentration or dilution value into the small box and click Add. The value is added to the larger box. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 136: Editing Sample Properties And Adding Samples With Sample Properties
Wizard dialog. Editing sample properties You can edit the properties of the samples or define new samples using the and adding samples with Sample Properties panel. Sample Properties ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 137: Adding New Samples To The Plate Layout
Use the Ctrl key to select several areas or to select wells one by one. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 138 Adding samples to the plate layout using Fill Wizard” on page 128). Enter the rest of the sample information in the fields. The same information is given to all the samples in the group. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 139: Creating New Groups For The Samples
Select the well(s) with the sample in the Plate Layout panel. You only have to select one of the wells because all the replicates are given the same information. Select the Properties tab in the Sample Properties panel. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 140: Modifying Group Names
Select the well(s) with the sample in the Plate Layout panel. You only have to select one of the wells because all the replicates are given the same information. Select the Properties tab in the Sample Properties panel. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 141: Adding Additional Information To Replicates
Enter the value and click OK. The property is added to the list of sample details. To edit the value of a property: Select the value of the property from the list of sample details. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 142: Printing The Plate Layout
Preview... - Select how many pages you want to view at a time. This selection will not affect the printing. • Page Setup... - You can define paper size, paper source, orientation and margins for printing. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 143 Plate Layouts Printing the plate layout • Font... - Select the font and font size used in the plate layout. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 144 Plate Layouts Printing the plate layout ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 145: Chapter 9 Calculations
Select the measurement for which you want to define calculations. Select the calculation from the Data Processing menu, click the button of that calculation on the action panel or right-click the measurement in the Results tree. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 146 Select the one you want to add. The software allows you to add only certain calculation steps for different measurement types. Table 9-1 shows the required data reductions for different measurement types. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 147: Deleting Calculations
Select a calculation from the Results tree and right-click it. Select Delete from the menu that opens next to the calculation. • Press the Delete button on your keyboard. • Select Edit > Delete or click the button. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 148 Calculations Deleting calculations ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 149: Chapter 10 Data Processing Parameters
See Example 10-2, Plate blank subtraction”. Specific blanks are subtracted from their own samples. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 150: Examples Of Blank Subtraction
The blank average is: (0.01 + 0.02)/2 = 0.015 The blank subtracted data would then be (with an accuracy of three decimals): Plate 1 -0.005 0.285 0.385 0.485 Plate 2 0.005 0.335 0.435 0.535 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 151: Blank Subtraction In Kinetic Measurements
Note If a certain rate cannot be determined, for example, increasing maximum rate if the whole reaction is decreasing, the software returns NaN (not a number). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 152: Basic Statistics
• Sample statistics with dilution factors – Calculates the statistics per sample while combining data from different dilutions and replicates ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 153: Data Normalization
Select Data Processing > Data Normalization, click the button on the Results action panel, or right-click the measurement in the Results tree. • Calculation - Select either Ratio or Inhibition. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 154: Multipoint Calculation
Standard deviation - The standard deviation of all the measurement points in each well. • Sum - The sum of all the measurement points in each well. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 155: Quantitative Curve Fit
Quantitative curve fit Select Data Processing > Quantitative Curve Fit, click the button on definitions the Results action panel, or right-click the measurement in the Results tree. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 156 To save the data as a new standard curve for use in other sessions: Select the groups for calibrators and unknowns. Select a correct wavelength. Only information related to the selected wavelength is saved. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 157: Table 10-1 Number Of Calibrators Required In Different Curve Fit Types
Cubic spline Four parameter logistic Log-Logit • Transformation - Select the type of data transformation trailing. Transformation of statistical data means in brief a change of scale used ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 158: Polynomial Fit Types
• y = a + bx + cx + dx + ex for the Quartic polynomial fit type. The minimum number of calibrators is five. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 159: Point To Point
Fill Wizard and the No. of replicates setting. Otherwise the cubic spline as a fit type in the quantitative curve fit calculation will not process correctly. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 160: Four Parameter Logistic And Log-Logit
. The list below the graph shows the calibrators used for defining the curve fit. The column Residual shows the difference between the calculated and actual values. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 161 Note If you want to remove a calibrator point or a replicate, you must disable it in the raw data. See the end of Raw measurement data” on page 203. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 162: Qualitative Classification
Any dilutions defined for samples in the layout are taken into account in the calculations. You can also select to ignore the dilutions in the calculations. Figure 10-1. Dividing measurement results into categories using cut-off limits ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 163 Limit to category check box for each desired category starting from the bottom (see Figure 10-1). Those categories become available and Qualitative Classification Limit Wizard is launched. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 164 • Color - Select a color for displaying each category value in the list view. Clicking the color field displays a small dialog for selecting the color. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 165 Data Processing Parameters Qualitative classification The result from the qualitative classification calculation is shown as the category text strings in the results list and table views. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 166: Effective Dose
Select Data Processing > Effective Dose, click the button on the Results action panel, or right-click the measurement in the Results tree. Select the Parameters tab to edit the calculation parameters. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 167 Group - Select the groups (assays) for both the controls and the unknowns used. The calculation is done for all unknown series found in the selected assay. • Sample - Select the individual sample. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 168 • Markers - Select whether the means of the measured calibrator replicates or individual measurements (replicates) are displayed in the curve. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 169: Quality Control (Qc)
Text if success - Enter a text string for each passed quality control check. The text string, for example, QC1 Succeeded, will be displayed for the quality control check value in the List view. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 170: Creating Formulas For Quality Control (Qc)
Quality Control calculation (see Quality control (QC)” on page 167). • Groups - Select which assay is used as the source data. The list includes all the available assays in the plate layout. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 171: User-Defined Equation
To add a user-defined equation step, select Data Processing > User-Defined Equation, click the button on the Results action panel, or right-click the measurement in the Results tree. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 172 Functions in user-defined equation”. When adding a function, click first the appropriate function button, then select the parameter(s) and close the parenthesis with the button. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 173 SQRT - Calculates the square root of the selected results. To calculate square root from the data, select [all groups in session] from the Group menu and [all samples in group] from the Sample menu. To calculate ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 174 Select either [all groups in session] or a specific group from the Group menu and [all samples in group] from the Sample menu. The calculation returns the average of all the samples either in the session or in the ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 175 The dilutions are taken into account. All the dilutions of a sample are multiplied by the dilution factor and the average is calculated. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 176: Spectral Analysis
The spectral peak search seeks all peak wavelengths with an intensity higher than the given threshold value. You can also define a wavelength area for which this calculation is performed (Figure 10-3). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 177: Figure 10-3 Spectral Peak Search: Threshold And Wavelength Area
(Figure 10-4 and Figure 10-5). Figure 10-4. Spectral peak search with window count 1 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 178: Spectral Maximum
You can define a wavelength area for which this calculation is performed. It is also possible to receive the maximum without defining the wavelength area or the threshold. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 179: Spectral Minimum
Wavelength 2 - Select the end wavelength for the scanning. Note User-selectable wavelength values are not checked against real measured values. Therefore, you may select wavelength values that are not even measured. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 180: Hbw Calculation
Figure 10-6. Definition of the HBW value If the 50% intensity value is not found from either lower or higher wavelengths, the calculation cannot be performed. See the following examples: ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 181: Figure 10-7 Hbw Calculation In A Simple Case
Figure 10-8. HBW calculation in a spectrum with two peaks In the case shown in Figure 10-8 the HBW calculation gives the same result for both peaks. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 182: Figure 10-9 Case Where An Hbw Calculation Is Not Possible
Therefore, you may select wavelength values that are not even measured. • Group - The calculation is performed for one replicate. Select the group to be used in the calculation. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 183: Ratio Within Spectrum
• Wavelength 1 - Select the wavelength of the first peak, I • Wavelength 2 - Select the wavelength of the second peak, I ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 184: Ratio Between Spectra
Wavelength 1 - Select the starting wavelength to be used in the Ratio between spectra calculation. • Wavelength 2 - Select the end wavelength to be used in the Ratio between spectra calculation. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 185: Select Wavelengths
Kinetic calculations are used to reduce data from kinetic measurements. Select Data Processing > Kinetic calculations, click the button on the Results action panel, or right-click the measurement in the Results tree. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 186: Average Rate
• Ignore first (n) readings - Ignores a defined number of points (readings), counted from the first reading. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 187: Maximum Rate
Window (2...100) setting. Figure 10-12. Determining the maximum rate with window value 2 You can define the following settings: ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 188 Ignore first (n) readings - Ignores a defined number of points (readings), counted from the first reading. • Ignore last (n) readings - Ignores a defined number of points (readings), counted from the last reading. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 189: Time To Maximum Rate
See Figure 10-13. Figure 10-13. Determining the time to maximum rate You can define the following settings: ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 190: Time To Maximum Rate / 2
187. This rate is halved and the data is scanned to determine the first rate, which is equal or exceeds this rate. The result is the time when this found rate occurs. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 191 Ignore first (n) readings - Ignores a defined number of points (readings), counted from the first reading. • Ignore last (n) readings - Ignores a defined number of points (readings), counted from the last reading. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 192: Time To Change
You can define the following settings: • Reaction - Define whether the reaction is supposed to produce increasing or decreasing signal levels. There are three options for this setting: ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 193: Maximum Of Well (Peak)
Maximum of well (Peak) The maximum of well is used to search for the maximum measurement value in each well. You can define the following settings: ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 194: Maximum - Minimum (Change)
Time to maximum (Peak) / The time to maximum / 2 equals the time to reach half of the maximum value. You can define the following settings: ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 195: Select Reading
Integral The integral calculation estimates the area under the measurement curve (Figure 10-14). The range of readings can be limited with the Ignore readings selection. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 196: Sum
In the example below (Figure 10-15), the kinetic processor sum gives the result (0.00 + 0.30 + 0.50 + 0.60 + 0.65 + 0.67) Abs = 2.72 Abs. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 197: Average Value
Ignore last (n) readings - Ignores a defined number of points (readings), counted from the last reading. Average value Average value is used to calculate the average value of all kinetic readings. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 198: Baseline Subtraction
5 to obtain 4 measurements from the non-reactive state and then do a baseline subtraction to ensure that the reaction in all wells starts from 0. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 199: Figure 10-17 Determining The Baseline Subtracted Kinetic Curve
Baseline - Select the number of points [Readings (0...100)], counted from the first or last reading, to be used as the baseline. If the number of readings is 0, no subtraction is made. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 200: Merge Data
Select Data Processing > Graph, click the button on the Results action panel, or right-click the measurement in the Results tree. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 201 The graph can be viewed when Graph is selected in the Results tree and the Graph tab is selected on the main window area. An example of an absorbance bar chart is shown below. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 202 Data Processing Parameters Graph For more information on how the graph is displayed, see Viewing results in graph format” on page 208. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 203: Trf Decay Calculation
Right-click menus” on page 50. Select Data Processing > TRF Decay Calculation, click the button on the Results action panel, or right-click the measurement in the Results tree. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 204 Data Processing Parameters TRF decay calculation • Sample - Select the sample for the Tau (t) and Alpha (a) calculation from the list. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 205: Chapter 11 Results
Other options are group name, sample type, dilution/concentration units and replicate number. Other viewing options are: ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 206 All the other measurement times are relative to that time. Other information in the list, shown in Table 11-1, depends on the measurement. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 207: Table 11-1 Result Information Shown In Result Lists
Reading in the kinetic loop The result list can be ordered in multiple ways. For more information, see Lists” on page 49 and Right-click menus” on page 50. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 208: Calculation Results
Other viewing options are: • Wavelength - If the measurement was performed with multiple wavelengths, select here the wavelength for which the calculation data ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 209: Viewing Results In List Format
HighPos”, for the qualitative classification result categories. The calculated effective dose values are displayed. The text string, for example, QC1 Succeeded”, will be displayed for the quality control categories. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 210: Viewing Results In Graph Format
Toolbar - Display the graph toolbar. • Data Editor - You can display the data points below the graph. • Legend Box - You can create a legend for the curve. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 211 Interlaced - Interlace the graph horizontally or vertically. • Properties... - You can more precisely define how the graph looks like. The example below shows the graph view of Quantitative Curve Fit. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 212 Calculation results For more information on curve fit results, see Quantitative Curve Fit results” on page 158. The example below shows the graph view of Effective Dose calculations. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 213: Viewing Results As Curves
Save step. To create a more detailed report of the entire run, you can use the Report step as described in ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 214 For example, you can create a folder for each year by adding the year placeholder to the file path and separating it with the backslash (\) character. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 215: Saving Manually
Report step to the Results tree after the measurements and calculations. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 216 Select the data from the list on the right and click the Format button. The button is not available for Step Data, Layout, Run Log, User Defined Graphs or QC Equations. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 217 Instrument serial number, Actual instrument temperature and Dispenser). You can also include laboratory information in the general data. The laboratory information includes the company contact information added ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 218 If you select Send via email to, enter the recipient's email address in the field. The measurement data is sent to the specified email address. For more information, see Email settings” on page 230. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 219 Measurement and calculation reports The PDF file is suitable for formal record keeping and it can be printed in color. The Excel file is sorted into separate worksheets. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 220 Results Measurement and calculation reports ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 221: Chapter 12 Instrument And Software Reports
The Start-up Initialization Status Report is needed for the instrument IQ/OQ/PQ. Select Settings > Instrument... > Setup... and then the Reports tab. Select the required report type using the radio buttons and click Run Report..® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 222 Instrument and Software Reports Creating instrument reports The Reports window is launched showing the selected report. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 223 The Export Report dialog is launched. Give a name to the report, select the file type and the folder and click Save. You can also print the report by clicking the button. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 224: Viewing Software Reports
You can view run status reports of a session. Select the session in the Results tree and the run status report opens in the main panel. reports ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 225 LumiSens module, and the plate tray used in the measurement. The actual instrument temperature is shown also. The report also has version information of SkanIt Software. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 226 Instrument and Software Reports Viewing run status reports ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 227: Chapter 13 Settings
Table 13-1. Available Settings menu items for different security groups Settings menu item Options Instrument Colors Plate Template Saved Curves Plate Tray Pathlength Correction Protocol Template Control Panel ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 228: Options
• Check plate - Tick to ensure that the instrument checks that a plate is inserted in the instrument before executing the session. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 229: Database
Database The Settings > Options... > Database tab is used to check and/or change the working database used by SkanIt Software (see Appendix B: Database maintenance”). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 230 If you want to view or take into use sessions or runs from an archived or restored database, first connect to that database using the procedure described ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 231: Temperature
Table view and the reports. The original accuracy of the data is retained in the memory and used in the calculations. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 232: Email Settings
The settings are needed, if you want to send, for example, measurement and calculation data reports to an email address automatically after executing a session. For more information, see Measurement and calculation reports” on page 213. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 233: Instrument
Enter the email address of the sender. Click the OK button to save the information. Instrument Open the Instrument Settings dialog by selecting Settings > Instrument..® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 234 Reports” on page 236 describes the Instrument Setup dialog. Also see Options” on page 226. To add a new instrument to the database, see Adding an instrument to the PC database” on page 34. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 235: General
The type label is on the right rear of the instrument. Communications Define the communication settings between the instrument and the PC on the Communications tab. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 236: Dispensers
19200. The recommended speed is 9600. This selection is only available for the COM port. Dispensers The Varioskan can be equipped with one optional dispenser, allowing dispensing from 1 to 1,000 µl in 1 µl increments. For configuring the dispenser of the instrument, select Settings >...
Page 237 The dispenser information is used in the dispensing step. For more information on using dispensing in protocols, see Dispense” on page 120. • Dispenser - The Varioskan instrument and SkanIt Software have one optional dispenser available; Dispenser 1. ® ®...
Page 238: Reports
Click OK. Reports You can create instrument status reports on the Reports tab. For more information, see Creating instrument reports” on page 219. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 239: Colors
Results table view. Select Settings > Colors..The Group Colors dialog is launched. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 240: Plate Template Settings
Note To use a newly created plate template, you have to first associate the plate template with one or several plate trays. For more information, see Associating plate templates with plate trays” on page 245. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 241: Creating Plate Templates
Select the new plate template from the list of plate templates and click Modify..The Plate template editor opens. Modify the dimensions of the plate template as necessary (see Modifying plate templates” on page 240). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 242: Modifying Plate Templates
Name - The name of the plate. • Well count X - The number of wells in the X-direction. • Well count Y - The number of wells in the Y-direction. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 243 The plate trays are associated with plate templates using the Plate Tray Editor (see Associating plate templates with plate trays” on page 245). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 244: Plate Tray
The database includes association links between plate types and plate trays. You can add new plate trays to the database and include compatibility information. You can also connect new plate templates to compatible plate trays. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 245 New... - Add a new plate tray to the database (see Adding new plate trays to the database” on page 244). • Modify... - Modify existing plate tray information (see Modifying plate trays” on page 246). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 246: Adding New Plate Trays To The Database
Type - Select the type of plate tray: Universal tray, Robotic tray or Specific incubation tray. • Lid - Tick the check box if the plate tray can be used with plates with a lid. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 247: Associating Plate Templates With Plate Trays
Select Settings > Plate Tray..The Plate Tray Editor dialog opens. Select the plate tray from the list and click Add..The Select Plate Template dialog opens. Select the plate template from the list and click OK. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 248: Modifying Plate Trays
Click Modify..The Modify Plate Tray dialog opens. Modify the information as necessary. For description of the settings, see Adding new plate trays to the database” on page 244. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 249: Editing Pathlength Correction K-Factors
Solvent list - List of solvents and their pathlength correction factors with descriptions. • Duplicate... - Make a copy of an existing pathlength correction factor and save it with a new name. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 250 K-Factor - Enter the pathlength correction factor value (from literature or the measurement). • Description - An optional description. Click OK to enter the new pathlength correction factor to the database. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 251: Saved Curves
Remove - Removes a curve from the list of saved curves. Select the curve you want to delete and click the button. Note You can only delete curves that are not in use. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 252: Protocol Template Setup
User-defined protocol (see Creating a new session” on page 61). • Add > - Add the selected protocol to the template list. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 253 < Remove - Remove the selected template from the template list. • Edit Name... - Click to change the name of the protocol template. Enter a new name for the protocol template and click OK. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 254: Skanit Control Panel
SkanIt Control Panel has the following views: Users (see Users” on page 253), Groups (see Groups” on page 256), General Settings (see ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 255: Users
Click the Change Password button to launch the Change Logon Password dialog. The button is only available, when the Password never expires check box is not selected. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 256: Viewing User Information
Only an administrator can view and modify the user information of other users (see Users” on page 275). In SkanIt Control Panel select your own user name and click Properties. The User Properties dialog is launched. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 257 Password never expires - If this is checked, the password does not expire. • Change Password - Click the button to launch the Change Logon Password dialog. The button is only available, when the Password never ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 258: Groups
Use the general settings to view and modify general logon and signature password settings. Only an administrator can modify these settings (see General settings” on page 282), other users may only view them. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 259: Company Settings
The technology approach is an event-based messaging system built on the .NET Framework version 2.0. SkanIt Automation Server is started by the Automation System and it has no graphical user interface. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 260 TCP/IP network, too. A separate document, Thermo Scientific Automation Interface for SkanIt Software ver. 2.4 User Manual (Cat. no. N05302), is available upon request from Thermo Fisher Scientific.
Page 261: Chapter 14 Menus And Action Panels
• 3 - Steps action panel. It is available when Protocol is selected. For more information about the buttons, refer to Steps action panel” on page 264. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 262: Menu Commands
Import/Export - Saves (exports) selected sessions and/or runs in separate files that can be imported to a database of another PC with SkanIt Software for Varioskan. Imports selected data from another PC with SkanIt Software for Varioskan (see Exporting and importing sessions”...
Page 263: Steps
(see Options” on page 226). • Instrument... - Launches the Instrument Setup dialog. You can configure the instrument connected to SkanIt Software and set the ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 264: Electronic Signatures (Dde Only)
(see Audit trail” on page 300) and signatures can be defined (see Electronic signature” on page 293). Electronic Signatures (DDE • Sign... - Sign a document electronically (see Signing a session or a run” only) on page 298). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 265: Execute
Contents... - Launches the Help application (see Chapter 15: Using Help”). • About... - Contains information about SkanIt Software and lets you create software reports (see Viewing software reports” on page 222). ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 266: Action Panels
Menus and Action Panels Action panels Action panels This section includes descriptions of all the action panel buttons in SkanIt Software for Varioskan. Session action panel The action panel has the following buttons: New session ( Creating a new session” on page 61) Open session ( Opening an existing session”...
Page 267: Results Action Panel
Graph ( Graph” on page 198) TRF Decay Calculation ( TRF decay calculation” on page 201) Report / Export ( Measurement and calculation reports” on page 213) ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 268: Report Toolbar
Run the plate into the instrument Run the plate out of the instrument Prime the dispenser tubing Empty the dispenser tubing Start the session execution (Chapter 5: Executing a Session”) ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 269: Chapter 15 Using Help
You can launch the Help application by selecting Help > Contents... from the menu bar, pressing the F1 key on your keyboard, or clicking the button on the action panel. The following Thermo Scientific SkanIt Software Help window appears. The Help toolbar buttons are: •...
Page 270 Using Help • Favorites – Add shortcuts to help topics of your choice. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 271: Chapter 16 Troubleshooting Guide
The arguments of the received Contact service. command are not valid. The X carriage position is Contact service. incorrect. The Y carriage position is Contact service. incorrect. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 272 Contact service. position is incorrect. Excitation selector position is Contact service. incorrect. Emission selector position is Contact service. incorrect. Varioskan LumiSens filter Contact service. position is incorrect. Excitation bandwidth selector Contact service. position is incorrect. Excitation beam diameter Contact service.
Page 273 XY table position calibration Contact service. failed. No factory calibration for the Contact service. current measurement method. This is only reported in response Contact service. to the factory calibration. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 274 Troubleshooting Guide Error messages Code Explanation Suggested action Varioskan LumiSens module Contact service. calibration failed. No tray attached to the Attach a tray to the tray holder. instrument. The tray carriage will not move unless there is a tray. Too high background level. The...
Page 275: Warning Messages
Invalid TRF measurement Contact service. command. Varioskan LumiSens crosstalk Clean the Varioskan LumiSens base. shield position is incorrect. Contact service if the error persists. Varioskan LumiSens reference Contact service. light failure. Dispensing head is not in the Move the dispensing head into the correct position.
Page 276 Dark level interpolation buffer Reduce well group size, use dark level overflow. interpolation between wells or do not use interpolation at all. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 277: Appendix A Administrators
The dialog lists the current users and the user groups they belong to that are defined in the database. Adding a new user Only an administrator can add new users. Click New User. The Add User dialog is launched. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 278 Password never expires - Tick the check box to avoid password expiration (defined in General Settings, General settings” on page 282). • Password - Enter the password. The user can later change the password. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 279: Modifying User Information
The Audit trail (DDE only) also records the full name. The full name cannot be edited after the user has been created. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 280: Unlocking User Accounts
Only an administrator can unlock a user account, and if necessary, change the user's forgotten password. In SkanIt Control Panel select the user whose account is locked and click Properties. The User Properties dialog opens. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 281: Groups
Only the administrator can add new user groups and modify their rights. Other users may only view the group list. The security rights defined for the different user groups are given in Table A-1. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 282: Table A-1 Rights Of The Different Security Groups
Create, delete or modify security groups Create an electronic signature and sign data Export result data To add a new user group, click New Group. The Create Group dialog opens. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 283 When a group is selected from the Control Panel Group list and the Properties button is pressed, the rights defined for the group can be viewed and modified. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 284: General Settings
You can give information here on the company (laboratory) performing the measurements. This information will be displayed in the reports (see Measurement and calculation reports” on page 213). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 285 • Company name, Tel., E-mail and Address - Enter the name and contact information of the company. • Save - Click the button to save the information. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 286 Appendix A Administrators Company settings ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 287: Appendix B Database Maintenance
Close SkanIt Software before you perform any database maintenance operations. Select Start > Programs > Thermo SkanIt Software > SkanIt RE/DDE for Varioskan Database Maintenance. • Database Administrator Settings - Give the database administrator login name, usually sa” and password as they were given during installation (see Step 1 in Installing software prerequisites”...
Page 288: Backup
Click the Backup button. You will be notified when the backup file is ready. A backup file can be restored to a database using the Restore function. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 289: Restore
That is, when you are ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 290: Archive
290. • Up to and including date - Choose the date up to which all the data will be archived (the selected date included). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 291: New Database
During this time, the Database Maintenance may appear to stop working but you should wait patiently. You will be notified when the database has been created. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 292: Upgrade
Database server name field. The location is defined at installation (see Step 7 in Installing the software” on page 26). ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 293 Click the Save button to change the database. Exit SkanIt Software. 10. Launch the Database Manager. When you now click the Connect button, the Database Manager is connected to the database of your choice. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 294 Appendix B Database maintenance Changing the working database ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 295: Appendix C Drug Discovery Edition With 21Cfr11 Compliance
The signature is always printed in all custom-made reports. The signature is shown in the Open Existing Session/Run dialog. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 296: Creating A Signature Password
SkanIt Control Panel from the Start menu. The Log On To SkanIt Control Panel dialog is displayed. Enter your current user name and password and click OK. The SkanIt Control Panel is launched. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 297 Appendix C Drug Discovery Edition with 21CFR11 Compliance Electronic signature Select the Users tab. Select the user you want to create the signature password for and click Properties. Click the Create Signature tab. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 298 The Create Signature tab has changed into the Signatures tab, the state is now Signature password is created and the Create Password button has changed into Change Password. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 299: Changing The Signature Password
Properties. All users can change their own signature passwords. Select the Signatures tab and click the Change Password button. The Change Signature Password dialog is opened. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 300: Signing A Session Or A Run
Select Electronic Signatures > Sign..The Select Document Type dialog opens. Select whether you want to sign the session or the run. Click OK. The Signatures for <session name> session/run dialog is opened. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 301: Viewing Signatures
Viewing signatures When you open a session or a run, click on the View Signatures button to view which user has signed the session or the run. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 302: Date And Time Format
Audit trail The audit trail shows who did what and when and what kind of operation was carried out in the system. The following operations are audited: creation, ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 303 – Tick one or more of the check boxes if you want to limit the number of objects shown according to the creation and/or modification time. • Show – Click the button to show the audit trail information. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 304 Instrument type, Instrument, User's access rights and Plate Template). • Creator – The creator of the original object. • CreationDateTime – The date and time of the creation of the original object. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 305: Glossary
Interfering signal from neighboring wells. EMU Emulation mode. DDE See Drug Discovery Edition. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 306 (generally ED ”), it denotes the dose having such an effect on a certain percentage (e.g., 50%) RFU or rfu See relative fluorescence/fluorometric of the test animals. units. ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 307 TRF delay Waiting period between the excitation flash end and the beginning of the emission light measurement. USB Universal serial bus. well An individual reaction vessel in a plate. ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 308 Glossary ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 309: Index
300 User-Defined Equation 169 autolock software after no usage 226 Calculations 261 automatic tip priming 120 calculations results automation interface 257 graph format 208 AutoRange 99, 103 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 310 238, 239 decreasing reaction 185 plate template 238, 239 default plate template 238 reports 211 selecting 61 signature password 294 defining software reports 222 calculations 143 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 311 99, 101, 103, 107, 109 in tree view 55 empty protocol 61 managing 55 empty well 125 moving 57 adding to the plate layout 128 renaming 57 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 312 160 .NET Framework 23 limits to categories 160 database engine 23 linear regression (LLS) fit type 156 software 26 list order 49 software prerequisites 23 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 313 234 Email settings 230 group names 138 general 226 instrument setup 34, 231 number formats 229 password settings 256, 282 temperature 229 pathlength correction K-factors 247 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 314 65 saving 63 importing 66 pull-back volume modifying 240 of the dispenser tip 234 selecting 61, 81 plate tray 242, 244, 245, 246 adding to database 244 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 315 213 serial port 37 exporting 213 series 131 printing 213 service group 256, 279 saving 211 service report 219 tree 143, 143 service status reports 219 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 316 SkanIt Software 54 signing a session or a run 298 startup options 226 size 81, 87 status bar 41 of execution block 81, 87 status reports 219 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 317 55 software reports 222 tree view 55, 55, 57, 57, 58, 59, 83, 143 user information 254 Results 143 volume 120 Steps 83 TRF decay 201 ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual...
Page 318 43 protocol editing 42 results 45 run log 48 window count 174 working database changing 290 xenon lamp 113 XLS file type 213, 219, 222 ® ® Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 319 Notes ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual 317...
Page 320 Notes ® ® 318 Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 321 Notes ® ® Thermo Fisher Scientific Thermo Scientific SkanIt Software for Varioskan User Manual 319...
Page 322 ® ® 320 Thermo Scientific SkanIt Software for Varioskan User Manual Thermo Fisher Scientific...
Page 324 Thermo Fisher Scientific Oy Microplate Instrumentation Ratastie 2, P.O. Box 100 FI-01621 Vantaa Finland www.thermo.com N02723...

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