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Summary of Contents for Thermo Scientific TSQ Endura
- Page 1 TSQ Series TSQ Endura and TSQ Quantiva Hardware Manual 80100-97014 Revision C February 2017...
- Page 2 EASY-Max NG, Ion Max NG, and Viper are trademarks; Unity is a registered service mark; and Hypersil GOLD AQ, Thermo Scientific, TSQ Endura, TSQ Quantiva, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Fisher Scientific is a registered trademark of Fisher Scientific Co.
- Page 3 Regulatory Compliance Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in the next section or sections by product name. Changes that you make to your system may void compliance with one or more of these EMC and safety standards.
- Page 4 Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one person alone to handle safely.
- Page 5 WEEE Directive 2012/19/EU Thermo Fisher Scientific is registered with B2B Compliance (B2Bcompliance.org.uk) in the UK and with the European Recycling Platform (ERP-recycling.org) in all other countries of the European Union and in Norway. If this product is located in Europe and you want to participate in the Thermo Fisher Scientific Business-to-Business (B2B) Recycling Program, send an email request to weee.recycle@thermofisher.com with the following information:...
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Page 7: Table Of Contents
Vent Valve........... 26 Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... - Page 8 Checking the Gas Supplies ........58 viii TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
- Page 9 Index ..............93 Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual...
- Page 10 Contents TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
- Page 11 Figure 22. Quadrupole Q1 or Q3 (TSQ Endura MS) ......36 Figure 23.
- Page 12 Removing the multipole MP00 and lens L0 assembly ....73 Figure 37. Removing the RF lens and exit lens (TSQ Endura MS) ....74 Figure 38.
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Page 13: Preface
Preface The TSQ Endura and TSQ Quantiva Hardware Manual describes the modes of operation and hardware components for the Thermo Scientific™ TSQ Endura™ and TSQ Quantiva™ systems. It also provides the instruments’ cleaning and maintenance procedures. This guide is intended for the following Thermo Scientific mass spectrometers (MSs): •... -
Page 14: Special Notices, Symbols, And Cautions
Note Highlights information of general interest. Highlights helpful information that can make a task easier. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... - Page 15 Sharp object: Avoid handling the tip of the syringe needle. Trip obstacle: Be aware of cords, hoses, or other objects located on the floor. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual...
- Page 16 Leaking liquids might contact the electronic components and cause an electrical short circuit. CAUTION Hot surface. Allow heated components to cool to room temperature (approximately 20 minutes) before servicing them. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
- Page 17 CAUTION Follow the instrument maintenance instructions. To avoid personal injury or damage to the instrument, do not perform any servicing other than that contained in the TSQ Endura and TSQ Quantiva Hardware Manual or related manuals unless you are authorized to do so.
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Page 18: Contacting Us
Go to thermofisher.com/us/en/home/technical-resources. To find product information Go to thermofisher.com/us/en/home/brands/thermo-scientific. Note To provide feedback for this document, go to surveymonkey.com/s/PQM6P62 or send an email message to Technical Publications (techpubs-lcms@thermofisher.com). xviii TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 19: Introduction
Introduction The TSQ Endura and TSQ Quantiva mass spectrometers are members of the Thermo Scientific family of mass spectrometers. The TSQ Endura and TSQ Quantiva systems consist of the mass spectrometer, a syringe pump, a divert/inject valve, and the Thermo Xcalibur data system. -
Page 20: Mass Spectrometer Models
TSQ Quantiva MS The TSQ Quantiva is a mass spectrometer that contains a triple-quadrupole mass analyzer and includes an external syringe pump, a divert/inject valve, and the Thermo Scientific Ion Max NG™ API source. The instrument requires two forepumps. TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 21: Overview Of An Lc/Ms Analysis
LC pump. The TSQ Endura and TSQ Quantiva MSs consist of an API source, ion optics, a triple-stage mass analyzer, and an ion detection system. The ion optics, mass analyzer, ion detection system, and part of the API source are enclosed in a vacuum manifold. - Page 22 The data system serves as the user interface to the mass spectrometer, autosampler, LC pump, and syringe pump. Refer to the Xcalibur Help for more information about the TSQ Endura MS or TSQ Quantiva MS data processing and instrument control application.
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Page 23: Lc/Ms Functional Block Diagram
LC/MS Functional Block Diagram Figure 1 shows a functional block diagram of the TSQ Endura and TSQ Quantiva systems with hyperlinks to the applicable sections. A sample transfer line connects the LC inlet device to the mass spectrometer. The LC device is usually installed on the left side of the mass spectrometer. -
Page 24: Electronic Assemblies
LED indicators on the instrument front panel. See their descriptions in Table Figure 2. TSQ Endura and TSQ Quantiva front panel LEDs Table 3. LEDs for the TSQ Endura and TSQ Quantiva MSs (Sheet 1 of 2) State Description Power Green The mass spectrometer is receiving power. -
Page 25: Power Entry Module
Introduction Controls and Indicators Table 3. LEDs for the TSQ Endura and TSQ Quantiva MSs (Sheet 2 of 2) State Description Vacuum Green The vacuum is within the allowable operating range. Yellow The system bakeout is in progress or the vacuum is outside the allowable operating range. -
Page 26: Communications Panel
For more information about resetting the mass spectrometer, see “Resetting the Mass Spectrometer” page Figure 4 shows the communication connectors, and Table 4 lists the pin-out descriptions for these connectors. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 27: Figure 4. Communication Connectors (Right Side Of The Ms)
Introduction Controls and Indicators Figure 4. Communication connectors (right side of the MS) Ethernet port USB ports Analog Input connector Peripheral Control connector Reset button Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... - Page 28 Provides a relay-driven programmable output signal to the connected external device, such as a fraction collector. Output: Maximum 24 Vdc, 3 A RO/IH Common (return) connection for the Ready Out and Injection Hold pins TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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Page 29: Cooling Fans
Chapter 8, “Maintenance.” CAUTION To ensure safety and proper cooling, always operate the mass spectrometer with its covers in place. This is also necessary to comply with product safety and electromagnetic interference regulations. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... - Page 30 Introduction Cooling Fans TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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Page 31: Chapter 2 Scan Parameters
Scan Parameters This chapter describes some of the scan parameter settings that you set in the TSQ Endura or TSQ Quantiva Tune application. Contents • Scan Types • Scan Mass-To-Charge Ratio Ranges • Data Types • Ion Polarity Modes Scan Types The TSQ Endura and TSQ Quantiva mass spectrometers operate in a variety of scan types. -
Page 32: Full Scan Q1 And Q3 Scan Types
The other rod assemblies (Q2 and Q3, or Q1 and Q2, respectively) act as ion transmission devices. The full scan Q1 scan type uses Q1 as the mass analyzer; the full scan Q3 scan type uses Q3 as the mass analyzer. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 33: Selected Ion Monitoring Scan Type
After Q1 selects the precursor ions, they enter Q2, which is surrounded by the collision cell. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 34: Figure 5. Illustration Of Product Scan Type
A mass spectrum obtained in the product scan type (product mass spectrum) is the mass spectrum of a selected precursor ion. Figure 5 illustrates the product scan type. Figure 5. Illustration of product scan type RF Only + Ar Q1 Set Q3 Scanning Q3 m/z TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 35: Precursor Scan Type
(for example, substituted aromatics, phthalates, steroids, or fatty acids) that have a common fragment ion (for example, m/z 149 for the phthalates). Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 36: Neutral Loss Scan Type
However, you frequently lose neutral moieties from substituent functional groups (for example, CO from carboxylic acids, CO from aldehydes, HX from halides, and H O from alcohols). TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 37: Figure 7. Illustration Of The Neutral Loss Scan Type
Figure 7. Illustration of the neutral loss scan type RF Only + Ar Q3 = Q1 - Q1 Scanning Q1 m/z Figure 8. Examples of compounds with a common neutral-loss fragment Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 38: Selected Reaction Monitoring Scan Type
TSQ Quantiva MS can operate in a mass-to-charge ratio range of m/z 10–1800. Data Types With the TSQ Endura or TSQ Quantiva MS, you can acquire and display mass spectral data (intensity versus mass-to-charge ratio) in one of two data types: •... -
Page 39: Ion Polarity Modes
Ion Polarity Modes Ion Polarity Modes The TSQ Endura and TSQ Quantiva MSs can operate in either positive or negative ion polarity mode. The mass spectrometer controls whether positive ions or negative ions are transmitted to the mass analyzer for mass analysis by changing the polarity of the voltage potentials applied to the API source, ion optics, and ion detection system. - Page 40 Scan Parameters Ion Polarity Modes TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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Page 41: Vacuum System
The vacuum system evacuates the region around the API source interface, ion optics, mass analyzer, and ion detection system. Figure 9 shows a functional block diagram of the vacuum system with hyperlinks to the applicable sections. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 42: Schematic Of The Internal Gas Supply Lines
Figure 10. Schematic of the internal gas supplies source Collision cell Source PCB Gas box (Left side of the MS) UHP argon gas inlet HP nitrogen gas inlet TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 43: Inlet Gases Hardware
Note For a list of guidelines for the operating parameters, refer to section “LC Flow Rate Ranges” in Chapter 1 of the TSQ Endura and TSQ Quantiva Getting Started Guide. •... -
Page 44: Nitrogen Gas Valves
When power to the vent valve solenoid shuts off for more than a very brief period of time, the vent valve opens and the manifold vents air. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 45: Vacuum Manifold
A collector attracts positive ions formed in the ion gauge. The collector current is related to the pressure in the vacuum manifold. The ion gauge is also involved in vacuum protection. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 46: Vacuum Pumps
Vacuum System Vacuum Pumps Vacuum Pumps The mass spectrometer requires either one (TSQ Endura MS) or two (TSQ Quantiva MS) external forepumps, and both instruments require an internal turbomolecular pump provide the vacuum pressures for the five vacuum regions (Figure 9). -
Page 47: Atmospheric Pressure Ionization Source
The API source also serves as the interface between the LC and the mass spectrometer. You can configure the EASY-Max NG API source (provided with the TSQ Endura MS) or the Ion Max NG API source (provided with the TSQ Quantiva MS) for the following ionization techniques:... -
Page 48: Api Source Interface
API spray insert while transferring them into the vacuum system. The regular ion transfer tube has a 0.58 mm (0.02 in.) diameter orifice (TSQ Endura MS) and the high-capacity ion transfer tube has a vertical 2 × 0.6 mm (0.08 × 0.02 in.) rectangular orifice (TSQ Quantiva MS). -
Page 49: Figure 15. Ion Transfer Tubes
The RF lens is an ion transmission device consisting of progressively spaced, stainless-steel electrodes. The RF lens differs slightly between the TSQ Endura MS and the TSQ Quantiva MS. The mass spectrometer applies an RF voltage to the electrodes, and adjacent electrodes have voltages of opposite phase. -
Page 50: Figure 16. Rf And Exit Lenses For The Mass Spectrometers
Vacuum System API Source Interface Figure 16. RF and exit lenses for the mass spectrometers TSQ Endura MS RF lens Exit lens TSQ Quantiva MS RF lens Back Front TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 51: Chapter 4 Ion Transmission And Mass Analysis
Dual-Mode, Discrete-Dynode Ion Detection System Ion Optics Figure 17 shows a schematic of the ion transmission path through the TSQ Endura and TSQ Quantiva MSs with hyperlinks to the applicable sections. Figure 17. Schematic of the mass spectrometer ion transmission path... -
Page 52: Mp00 Ion Optics
L0 to aid in ion transmission. Lens L0, which mounts to the MP00 RF lens, also acts as a vacuum baffle between the MP00 and MP0 ion optics chambers. Figure 19. Lens L0 TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 53: Mp0 Ion Optics
Lenses TK1 and TK2 as a vacuum baffle between the multipole MP0 and quadrupole Q1. Figure 21. TK1 (left) and TK2 (right) lenses Front Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 54: Mass Analyzers
(Figure 17). Quadrupoles Q1 and Q3 enable high-resolution scans without signal loss. For the TSQ Endura MS, quadrupoles Q1 and Q3 (also known as a hyperquads) are square arrays of circular rods (Figure 22). For the TSQ Quantiva MS, quadrupoles Q1 and Q3 are... -
Page 55: Applied Rf And Dc Fields
Figure 25. Polarity of the RF and DC voltages applied to the Q1 and Q3 mass analyzers RF voltage + DC voltage RF voltage 180° out of phase – DC voltage Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 56: Figure 26. Magnitude Of The Asymmetric Rf And Dc Voltages Applied To The Q1 And
(approximately 1.095 MHz for the TSQ Endura MS and 1.005 MHz for the TSQ Quantiva MS). The RF voltage applied to one rod pair has 90% of the amplitude and is opposite in sign of the other rod pair. -
Page 57: Mass Analysis
The mechanism of dissociation involves converting some of the TKE of the ion into internal energy. This collision places the ion in an excited state. If the internal energy is sufficient, the ion fragments. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 58: Quadrupole Offset Voltage
• To retain the collision gas. Lenses EL23 and EL31 form two of the walls of the collision cell, so they tend to hold the collision gas in the collision cell. However, the collision gas escapes through the same lens holes through which the ion beam passes. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 59: Dual-Mode, Discrete-Dynode Ion Detection System
Its purpose is to shield Q3 from the high voltage applied to the ion detection system and to shield the ion detection system from the high RF voltages applied to Q3. Dual-Mode, Discrete-Dynode Ion Detection System The TSQ Endura and TSQ Quantiva MSs have a high-sensitivity, dual-mode, discrete-dynode ion detection system (Figure 17). - Page 60 Ion Transmission and Mass Analysis Dual-Mode, Discrete-Dynode Ion Detection System TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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Page 61: Syringe Pump And Divert/Inject Valve
Syringe Pump and Divert/Inject Valve This chapter describes the external syringe pump and divert/inject valve that ship with the TSQ Endura or TSQ Quantiva mass spectrometer. For information about installing these components, refer to the TSQ Endura and TSQ Quantiva Getting Connected Guide. -
Page 62: Divert/Inject Valve
The Method Editor in the Xcalibur application identifies the valve’s two positions as “1–2” (port 1 to 2) and “1–6” (port 1 to 6). TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 63: Configurations
5 and exits through port 6 into the API source. • Waste (position 1–6)—Solvent flow from the LC pump enters the valve through port 5 and exits through port 4 to waste. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 64: Controlling The Divert/Inject Valve
For instructions, refer to the manufacturer’s manual. Figure 30. Divert/inject valve (front view) Valve position indicator Six-port, two-position valve Valve control buttons TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 65: System Shutdown, Startup, And Reset
System Shutdown, Startup, and Reset When you are not using the TSQ Endura or TSQ Quantiva system for short periods of time, place the mass spectrometer in standby mode. For longer periods, for example, two or more months, you can shut it down completely. In addition, many maintenance procedures for the system require shutting down the mass spectrometer completely. -
Page 66: Placing The Mass Spectrometer In Standby Mode
Placing the Mass Spectrometer in Standby Mode Placing the Mass Spectrometer in Standby Mode If you are temporarily not using the TSQ Endura or TSQ Quantiva MS, you do not need to shut it down completely. Instead, place the mass spectrometer in standby mode. -
Page 67: Shutting Down The Mass Spectrometer Completely
Shutting Down the Mass Spectrometer Completely Shutting Down the Mass Spectrometer Completely Shut down the TSQ Endura or TSQ Quantiva system completely only when you are not using it for an extended period of time or when you must shut it down for maintenance or service. -
Page 68: Starting The System After A Complete Shutdown
4. If an autosampler and printer are included, use their On/Off switches to turn them off. Starting the System After a Complete Shutdown To start the TSQ Endura or TSQ Quantiva system after it has been shut down completely, follow these procedures: •... -
Page 69: Starting The Autosampler
5. If the mass spectrometer was turned off for an extended period of time, follow the procedures in “Pumping Down the Mass Spectrometer,” in Chapter 5 of the TSQ Endura and TSQ Quantiva Getting Started Guide. Otherwise, wait at least 1 hour to allow the mass spectrometer to pump down. -
Page 70: Resetting The Mass Spectrometer
(such as spray voltage, sheath and auxiliary gas flow, or ion transfer tube temperature). • If you reset the instrument to the factory calibration settings, always repeat the calibration of the internal electronic devices as specified in the TSQ Endura and TSQ Quantiva Getting Started Guide. Otherwise, all instrument calibrations might produce incorrect results. -
Page 71: Restarting The Data System
Restarting the Data System If possible, use the Windows restart procedure to shut down and restart the data system so that Windows can properly close applications and save changes to any open Thermo Scientific application. Note After you reset the data system, the communications link between the data system and the mass spectrometer is automatically reestablished. - Page 72 You can control this setting in your method even when the instrument is in standby mode. In standby mode, the Tune application sets the API gases to their standby default settings (2 arbitrary) to keep the API source clean. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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Page 73: Chapter 7 Daily Operation
Daily Operation To ensure the proper operation of the TSQ Endura or TSQ Quantiva system, Thermo Fisher Scientific recommends that you perform daily preventive maintenance. This chapter specifies the items to check before operating the system and the cleaning procedures to perform after completing the analyses. -
Page 74: Checking The System Mode
, indicates that the readback value is good. CAUTION For proper performance, operate the TSQ Endura or TSQ Quantiva system at the proper vacuum levels. Operating the system with poor vacuum levels can cause reduced sensitivity and reduced electron multiplier life. - Page 75 3. Check each fitting and flange on the system for tightness, and tighten the fittings or flanges that are loose. Do not tighten fittings indiscriminately. Pay particular attention to fittings that have been changed recently or to fittings that have been subjected to heating and cooling. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual...
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Page 76: Checking The Gas Supplies
Verify that the pressure of nitrogen gas reaching the mass spectrometer is 690 ±140 kPa (100 ±20 psi). If necessary, adjust the pressure with the tank pressure regulator. For more information about gas requirements, refer to the TSQ Endura and TSQ Quantiva Preinstallation Requirements Guide. -
Page 77: Purging The Oil In The Forepump
The best time to purge the oil is at the end of the working day after you flush the inlet components. Remember to close the purge valve before continuing normal operation. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 78: Emptying The Solvent Waste Container
Placing the System in Standby Mode After you complete the daily maintenance procedures, place the mass spectrometer in standby mode as described in “Placing the Mass Spectrometer in Standby Mode.” TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 79: Maintenance
TSQ Quantiva MS: sweep cone, ion transfer tube, API source interface, RF lens, MP00 RF lens, and lens L0. Unless otherwise noted, use the TSQ Endura MS procedures. CAUTION Heavy object. Never lift or move the instrument by yourself; you can suffer personal injury or damage the instrument. -
Page 80: Maintenance Schedule
Every 12 months of typical use, or if the oil is cloudy or discolored Cooling fans Clean the air filter. Every 4 months page 76 For instructions about maintaining the LC modules, refer to that instrument’s manual. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 81: Guidelines
(One of the tools is in the TSQ Source Installation Kit.) In addition, you can use the contents of the PM Cleaning Kit (P/N 70111-62112). Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... - Page 82 Screwdriver, Phillips #2 (M3) – (Optional) Toothbrush, soft (or similar tool) – (Optional) Tweezers, plastic (or similar tool) – Equipment Beaker or graduated cylinder (for use with – methanol) Chamois-tipped swabs 00725-01-00028 TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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Page 83: Maintaining The Api Source Housing
2. Put on appropriate eye-wear and gloves. 3. Under an appropriate fume hood, rinse the interior of the housing with UHPLC/MS-grade methanol. 4. Allow the housing to dry before you install it on the mass spectrometer. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 84: Maintaining The Api Source Interface
Follow these procedures: To remove the ion sweep cone and the ion transfer tube To clean the spray cone and O-ring To clean the ion transfer tube To clean the ion sweep cone TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 85: Figure 31. Ion Sweep Cone Removed From The Ms Mount Assembly
• To avoid contaminating the ion transfer tube, do not touch its exposed entrance. Figure 31. Ion sweep cone removed from the MS mount assembly Release lever for the API source interface API cone seal Ion sweep cone Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 86: Figure 32. Ion Transfer Tube Removal Tool (Tsq Endura Ms)
(flat edges) on the tool to slowly pull it out of the API source interface. Figure 32. Ion transfer tube removal tool (TSQ Endura MS) API source interface Fit this end of the tool around the exposed ion transfer tube. -
Page 87: Figure 34. Spray Cone, O-Ring, Ion Transfer Tube, And Ion Sweep Cone (Tsq Endura Ms)
1. Soak the lint-free tissues or chamois-tipped swabs in a 50:50 solution of methanol/water, and then clean the exterior surface of the spray cone. 2. (TSQ Endura MS) Remove and inspect the O-ring located in the spray cone under the entrance end of the ion transfer tube (Figure 34). - Page 88 Tip If you successfully unblocked the ion transfer tube, check that the Source Pressure reading has increased to a normal value (below 2 Torr for the TSQ Endura MS or below 4.5 Torr for the TSQ Quantiva MS). If trying this method does not clear the blockage, replace the ion transfer tube.
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Page 89: Removing The Api Source Interface
3. Remove the API source housing. 4. Lift up the release latch, grasp the API source interface with your fingers, and then carefully pull it out of the vacuum manifold (Figure 35). Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 90: Cleaning The Rf Lens, Exit Lens, Mp00 Rf Lens, And Lens L0
Maintenance Maintaining the API Source Interface Figure 35. API source interface removed from the vacuum manifold (TSQ Endura MS) Vacuum manifold Release latch on the API source interface Viton™ O-ring Cleaning the RF Lens, Exit Lens, MP00 RF Lens, and Lens L0 Chemicals can accumulate on the surfaces of the RF lens, exit lens, MP00 RF lens, and lens L0. -
Page 91: Figure 36. Removing The Multipole Mp00 And Lens L0 Assembly
4. Grasp the two retainer thumbscrews and carefully pull the RF lens (Figure 37) with the exit lens straight out of the API source interface cage. 5. Remove the exit lens off of the RF lens. Place both on a clean surface. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 92: Figure 37. Removing The Rf Lens And Exit Lens (Tsq Endura Ms)
Maintenance Maintaining the API Source Interface Figure 37. Removing the RF lens and exit lens (TSQ Endura MS) RF lens and exit lens assembly Exit lens 6. Remove multipole MP00 and lens L0 by pushing them out of the MP00-L0 mount cage. - Page 93 (Figure 37). 2. Reassemble the multipole MP00 and lens L0 assembly. 3. Attach the MP00-L0 assembly to the API source interface cage, and then attach lens L0 (Figure 36). Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual...
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Page 94: Reinstalling The Api Source Interface
Disconnect the plumbing tubing to the API source. ii. Depress the four spring catches that are located on either side of the front cover. iii. Pull the front cover off at an angle to clear the API source. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 95: Figure 39. Air Filter Location In The Mass Spectrometer With The Front Cover Removed
2. Wash the air filter in a solution of soap and water. 3. Rinse the filter with tap water, and then allow it to air dry. 4. Reinstall the air filter and front cover. 5. Reconnect the plumbing tubing. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... - Page 96 Maintenance Maintaining the Air Filter TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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Page 97: Diagnostics And Pcb And Assembly Replacement
Diagnostics and PCB and Assembly Replacement The TSQ Endura and TSQ Quantiva system diagnostics can test many instrument components. For example, the diagnostics can help you locate a problem with the instrument electronics. Usually, replacing a faulty PCB or assembly can correct the problem. After applying the fix, run the diagnostic tests again to verify that the instrument is functioning properly. -
Page 98: Replacing Fuses, Pcbs, And Power Supplies
CAUTION Thermo Fisher Scientific service is required. Because the electronic assemblies are close-packed to minimize the size of the instrument, only a service engineer can replace electrical components (that is, fuses, PCBs, power supplies, and so on). TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 99: Chapter 10 Replaceable Parts
Replaceable Parts This chapter provides the kit part numbers for the TSQ Endura and TSQ Quantiva MSs. Note You receive all of the listed kits with your ordered system, except for the TSQ Chemical Kit. The chemical kit arrives separately as part of the preinstallation kit. -
Page 100: Calibration Kit
(Upchurch Scientific 1535XL) Tubing, Teflon FEP, 1/16 in. OD, 0.030 in. ID, 3 cm (1.2 in.) 00301-22915 long (Upchurch Scientific 1522) Union, HPLC, black PEEK, 10-32, 0.01 in. thru-hole 00101-18202 (Upchurch Scientific P-742) TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 101: Ms Setup Kit
(Upchurch Scientific P-727) ....... 00101-18204 Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... -
Page 102: Single Mechanical Pump Kit
80100-60049 Fitting Tee, barbed, nylon, for 0.5 in. (12.7 mm) ID tubing 00103-01-00012 Tubing, Tygon, 3/4 in. (19.1 mm) OD, 0.5 in. (12.7 mm) ID, 6 m 00301-22920 (20 ft) long TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific... -
Page 103: Tsq Source Installation Kit
TSQ Source Installation Kit Ion transfer tube removal tool, TSQ Endura..... . . 70111-20258 Ion transfer tube removal tool, TSQ Quantiva ..... 70005-20972 L0 lens removal tool . - Page 104 2 μL, Peek ........... . 00110-16012 TSQ Endura and TSQ Quantiva Hardware Manual...
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Page 105: Glossary
(API) Ionization performed at atmospheric pressure by using collision gas A neutral gas used to undergo collisions atmospheric pressure chemical ionization (APCI), with ions. heated-electrospray (H-ESI), or nanospray ionization (NSI). Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... - Page 106 API ion transfer tube and acts also be referred to as a backing, mechanical, rotary- as a physical barrier to protect the entrance of the vane, roughing, or vacuum pump. tube. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
- Page 107 The peak-height level is usually specified as a of the molecular structure. percentage of the maximum peak height. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual...
- Page 108 In this type of device, virtually all ions have stable trajectories and pass through the assembly. syringe pump A device that delivers a solution from a syringe at a specified rate. TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
- Page 109 API stack, ion optics, mass analyzers, and ion detection system. vacuum system Components associated with lowering the pressure within the mass spectrometer. A vacuum system includes the vacuum manifold, pumps, pressure gauges, and associated electronics. Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual...
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Page 111: Index
API source housing exit lens lens L0 electromagnetic compatibility MP00 RF lens electron multiplier gain RF lens syringe electronic assemblies collision cell efficiency collision energy (Q2 offset voltage) Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual... - Page 112 MP00 RF lens, cleaning API source housing, cleaning MP00, description fan filter, cleaning ion polarity modes forepump oil, purging ion pressure vacuum gauge schedule mass analysis, discussion TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
- Page 113 MS components precursor O-rings product API source interface, under selected reaction monitoring (SRM) ion transfer tube, under summary of sheath gas, description shutdown emergency procedure parts mass spectrometer See replaceable parts Thermo Scientific TSQ Endura and TSQ Quantiva Hardware Manual...
- Page 114 LED description note about pressure vacuum manifold description pressures vacuum regions vacuum pressure levels, checking vacuum pumps See turbomolecular pumps vacuum system description functional block diagram gauges collision pressure convection TSQ Endura and TSQ Quantiva Hardware Manual Thermo Scientific...
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