Page 1 UltiMate 3000 Series SD, RS, and BM Pumps Operating Instructions (Original Operating Instructions) Revision: 1.4 Date: September 2010 © 2010 Dionex Doc. No. 4820.4001...
Page 2 UltiMate 3000 Series: SD, RS, and BM Pumps Operating Instructions...
Page 3: Declaration Of Conformity
ISO-3100SD, ISO-3100BM HPG-3200SD, HPG-3200RS, HPG-3400SD, HPG-3400RS LPG-3400SD, LPG-3400RS, LPG-3400BM DGP-3600SD, DGP-3600RS, DGP-3600BM Dionex GmbH herewith declares conformity of the above products with the respective requirements of the following regulations:  Machinery Directive 2006/42/EC  Low-Voltage Directive 2006/95/EC  EMC Directive 2004/108/EC The safety of the machinery was evaluated based on the following standard: ...
Page 4 UltiMate 3000 Series: SD, RS, and BM Pumps Page II Operating Instructions...
Page 5 UltiMate 3000 Series: SD, RS, and BM Pumps Operating Instructions Page III...
Page 6 UltiMate 3000 Series: SD, RS, and BM Pumps Page IV Operating Instructions...
Page 7 UltiMate 3000 Series: SD, RS, and BM Pumps Operating Instructions Page V...
Page 8 UltiMate 3000 Series: SD, RS, and BM Pumps Page VI Operating Instructions...
Page 9: Table Of Contents
UltiMate 3000 Series: SD, RS, and BM Pumps Table of Content Introduction........................1 How to Use this Manual ..................1 Safety Information ....................2 1.2.1 Symbols on the Pump and in the Manual ............2 1.2.2 General Safety Precautions................3 1.2.3...
Page 10 3.5.3 Configuring the Pump..................35 3.5.3.1 Initial Installation..................35 3.5.3.2 Changing the Configuration Properties ...........42 Connecting a Dionex ESA Detector to the Pump ..........43 Preparation for Operation (Startup) ................45 Overview of Actions ....................45 General Precautions for Connecting Capillaries..........47 Solvent Reservoirs ....................48 4.3.1 General Notes ....................48...
Page 11 UltiMate 3000 Series: SD, RS, and BM Pumps 5.5.3 Setting the Flow Rate, Flow Acceleration, and Flow Deceleration ....81 5.5.4 Setting the Pressure Limits................82 5.5.5 Recording the Pump Pressure................. 83 5.5.6 Rear Seal Wash System.................. 83 5.5.6.1 Working with Rear Seal Washing ............83 5.5.6.2...
Page 12 UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.2.1 Removing the Pump Head and Pistons..........135 7.5.2.2 Installing the Pistons and Pump Head ...........137 7.5.3 Replacing the Piston Seals................139 7.5.3.1 Disassembling the Pump Head and Removing the Piston Seals ...140 7.5.3.2 Cleaning the Pistons ................141...
Page 13 UltiMate 3000 Series: SD, RS, and BM Pumps HPG-3200 ......................176 8.4.1 Interior Components ..................176 8.4.2 Flow Path...................... 177 8.4.3 Operating Principle (Schematics)..............178 HPG-3400 ......................179 8.5.1 Interior Components ..................179 8.5.2 Flow Path...................... 180 8.5.3 Possible Gradient Combinations and Operating Principle (Schematics) ..181 Optimizing the Pump for Specific Applications ............
Page 14 UltiMate 3000 Series: SD, RS, and BM Pumps Page vi Operating Instructions...
Page 15: Introduction
The layout of this manual is designed to provide quick reference to the sections of interest to the reader. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation.
Page 16: Safety Information
Dionex. 1.2 Safety Information The CE Mark label and cTUVus Mark safety label on the pump indicate that the pump is compliant with the related standards (...
Page 17: General Safety Precautions
UltiMate 3000 Series: SD, RS, and BM Pumps At various points throughout the manual, messages of particular importance are indicated by certain symbols: Tip: Indicates general information, as well as information intended to optimize the performance of the instrument. Important: Indicates that failure to take note of the accompanying information could cause wrong results or may result in damage to the module.
Page 18  Always use fresh rear seal wash liquid.  Dionex advises against recycling the solvents. This may impair the performance of the seals.  When connecting the capillaries, make sure that the connectors are free from contaminants.
Page 19 The enclosure should be opened only by authorized service personnel.  Always replace blown fuses with original spare part fuses from Dionex ( page 191).  Replace faulty power cords and communication cables.
Page 20: Consignes Générales De Sécurité
Attendez jusqu'à ce que le rinçage du joint arrière ait été pompé par toutes les têtes de pompe.  Utilisez toujours le liquide frais pour le rinçage du joint arrière.  Dionex déconseille de recycler les solvants. Ceci peut nuire aux performances des joints. Page 6 Operating Instructions...
Page 21 électrique avant de retirer les capots. Ne démontez les capots de protection que si cela est explicitement demandé au cours de ces instructions.  Remplacez toujours les fusibles grillés par des fusibles de rechange d'origine Dionex ( page 191). ...
Page 22 97.  Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l'instrument. ...
Page 23: Intended Use
(AB Sciex), Compass™/ HyStar™ (Bruker Daltonics), or Xcalibur™ (Thermo Scientific), contact the Dionex sales organization. Please note that the pump may be operated only with Dionex accessories and spare parts ( page 191) and within its technical specifications ( page 187).
Page 24 UltiMate 3000 Series: SD, RS, and BM Pumps Page 10 Operating Instructions...
Page 25: Overview
2 Overview 2.1 Unit Description The pump is the heart of the Dionex UltiMate 3000 system. The pump performs equally well as a flexible and reliable module for routine analysis and sophisticated research tasks in HPLC and UHPLC and can be used in numerous laboratory environments: ...
Page 26: Pump Configurations
UltiMate 3000 Series: SD, RS, and BM Pumps 2.2 Pump Configurations 2.2.1 Overview The pump is available in the following configurations: Pump Description Part No. ISO-3100SD Isocratic pump (analytical, 1 solvent) 5040.0011 working pressure: up to 62 MPa (9000 psi)
Page 27: Combinations Of Ultimate 3000 Pumps And Solvent Racks
- one HPG-3400 - two HPG-3200s in a two-stack system - one HPG-3200 or ISO-3100SD if you want to degas the solvents and the wash liquid of an UltiMate 3000 series autosampler SRD-3600 Solvent Rack with analytical 6-channel vacuum degasser 5035.9230...
Page 28: Special Information For Biocompatible Pumps
UltiMate 3000 Series: SD, RS, and BM Pumps 2.2.3 Special Information for Biocompatible Pumps The UltiMate 3000 pump series includes the following biocompatible pump types:  LPG-3400BM  DGP-3600BM  ISO-3100BM Except for the fluid components, the BM pumps are identical to the SD and RS pumps.
Page 29: Operating Principle
UltiMate 3000 Series: SD, RS, and BM Pumps 2.3 Operating Principle The pump is a zero-pulsation, serial dual-piston pump with electronic compressibility compensation. The pump head includes two cylinders—working cylinder and equilibration cylinder—that are connected in series. The solvent passes both cylinders successively.
Page 30: Front Panel Elements
UltiMate 3000 Series: SD, RS, and BM Pumps 2.4 Front Panel Elements Fig. 1 Front panel view Front Panel Description Element Display Shows information about the pump, for example: - General information upon power up ( page 61) - Status screen ( page 62) - Functions and menus (...
Page 31: Rear Panel
UltiMate 3000 Series: SD, RS, and BM Pumps 2.5 Rear Panel Fig. 2: Rear panel view Description Power switch ( page 18) Fuse cartridge ( page 18) Main power receptacle ( page 31) Digital I/O ports for communication with external devices ( page 19) Solvent Rack port for connection of an SRD-3x00 Solvent Rack (...
Page 32: Power Switch
UltiMate 3000 product line, depending on the configuration of the UltiMate 3000 system, or three external USB hubs to the pump. Important: Use these USB ports only for connections to Dionex instruments. Dionex cannot guarantee correct functioning if instruments from other manufacturers are connected.
Page 33: Digital I/O
UltiMate 3000 Series: SD, RS, and BM Pumps 2.5.4 Digital I/O The digital I/O ports provide two inputs and two relay outputs that can be used to exchange digital signals with external devices. To connect external devices to the digital I/O ports, use the appropriate mini-DIN cables (part no.
Page 34: Fluid Connections
UltiMate 3000 Series: SD, RS, and BM Pumps 2.6 Fluid Connections The front panel door tilts upward to provide easy access to the fluid connections in the pump. Tilt the front cover upward. The open front panel locks in the topmost position.
Page 35: Rear Seal Wash System
UltiMate 3000 Series: SD, RS, and BM Pumps 2.7 Rear Seal Wash System As a standard, the pump is equipped with an active rear seal wash system. Rear seal washing helps avoiding damages to the pistons, piston seals, and support rings, and thus increases the piston life.
Page 36: Mixing System And Inline-Filter
UltiMate 3000 Series: SD, RS, and BM Pumps 2.8 Mixing System and Inline-Filter RS and SD pumps except ISO-3100SD All RS and SD pumps (except ISO-3100SD) are shipped with a two-step mixing system (SpinFlow). The system includes a capillary mixer and a static mixer. DGP-3600 pumps have to separate two-step mixing systems.
Page 37: Purge Unit
UltiMate 3000 Series: SD, RS, and BM Pumps 2.9 Purge Unit The purge unit comprises a pressure transducer for the system pressure and purge valve with purge valve knob and outlet nozzle. Connection port Connection port for the right pump head...
Page 38: Vacuum Degasser
Therefore, it is normally not required to use a degasser with these eluents If the UltiMate 3000 system includes an UltiMate 3000 series autosampler, you should degas also the wash liquid on a continuous basis. The procedure how to prepare and install the wash liquid lines is similar to the steps for the solvent supply (...
Page 39: Chromeleon Software
UltiMate 3000 Series: SD, RS, and BM Pumps 2.13 Chromeleon Software The pump can be controlled by the Chromeleon Chromatography Management System. To control the pump, an appropriate Chromeleon version and license are required. Two modes of software control are available: ...
Page 40: Wellness, Predictive Performance And Diagnostics
UltiMate 3000 Series: SD, RS, and BM Pumps 2.14 Wellness, Predictive Performance and Diagnostics System Wellness monitors the health of the pump. Therefore, the pump supports several performance and reliability features that can help you detect small problems before they turn into big ones: ...
Page 41: Installation
Tips: Immediately report any shipping damage to both, the incoming carrier and Dionex. Shipping insurance will compensate for the damage only if reported immediately Keep the original shipping container and packing material. They provide excellent protection for the pump in case of future transit.
Page 42: Positioning The Pump In The Ultimate 3000 System
UltiMate 3000 Series: SD, RS, and BM Pumps 3. Remove the foam spacers, and then remove the polythene packaging. 4. Tilt the front panel upward and remove the foam inserts securing the front panel door during shipment. 5. Before connecting the pump to the power source, wait approximately 4 hours to allow the instrument to come to room temperature and to allow any condensation that might have occurred during shipping to evaporate.
Page 43 Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer by using the USB port on the rear panel of the instrument. However, Dionex recommends interconnecting all modules, and then connecting the system to the Chromeleon computer with only one connection. For systems with a DAD-3000(RS) or MWD-3000(RS), you can use only the hub on the detector for the connection.
Page 44: Connecting The Pump
UltiMate 3000 Series: SD, RS, and BM Pumps 3.4 Connecting the Pump 3.4.1 General Information If you want to operate the pump with Chromeleon Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the pump to the USB port on the Chromeleon computer and turn on the pump power.
Page 45: Connecting The Power Cord
To connect devices to the digital I/O ports on the rear panel of the pump, use a 6-pin mini-DIN signal cable. The cable is available from Dionex (part no. 6000.1004). 1. Plug the 6-pin connector of the mini-DIN cable into the Digital I/O port 1 (or 2). For information about the functions of the connector pins and pin assignment, see page 215.
Page 46: Setting Up The Pump In Chromeleon
UltiMate 3000 Series: SD, RS, and BM Pumps 3.5 Setting Up the Pump in Chromeleon This section provides brief instructions for setting up the pump in Chromeleon. For details, see the Chromeleon Help. Tip: When the pump is connected to the Chromeleon computer, verify that the Chromeleon software is installed before turning on the pump power for the ®...
Page 47 UltiMate 3000 Series: SD, RS, and BM Pumps To resolve the problem: a) Click Cancel to exit the wizard. b) Turn off the pump. c) Install Chromeleon. d) Turn on the power to the pump. Windows will now detect the pump and install the USB software for the pump automatically.
Page 48: Installing The Pump
Add Device on the menu. 6. On the Manufacturers list, click Dionex HPLC: UltiMate 3000 and on the Devices list, click the pump you want to add, for example, HPG-3x00RS Pump or LPG- 3400SD Pump.
Page 49: Configuring The Pump
UltiMate 3000 Series: SD, RS, and BM Pumps 3.5.3 Configuring the Pump 3.5.3.1 Initial Installation On each configuration page for the pump, check and change the settings if necessary and select additional settings if needed. You may reopen the configuration pages later again to change the settings (...
Page 50 UltiMate 3000 Series: SD, RS, and BM Pumps  Module Address Select the module address of the pump if necessary. Click Browse and then double-click the pump that you want to use on the Device List. The address is automatically entered in the Module Address box.
Page 51 UltiMate 3000 Series: SD, RS, and BM Pumps Under PumpModule, the following commands and properties are displayed, for example, in the Commands dialog box ( page 65):  General pump properties and commands, such as, Connect, Connected, Disconnected, and Degasser.
Page 52 UltiMate 3000 Series: SD, RS, and BM Pumps  Timebase (only DGP-3600) Select the timebase to which the related the pump is assigned. The default setting is the timebase in which the pump is installed. To change the setting, click the arrow next to the Timebase box and click a different timebase on the list.
Page 53 UltiMate 3000 Series: SD, RS, and BM Pumps Limits Page or Left Limits and Right Limits pages (depending on the pump type) Displays the allowed ranges for the flow rate and the pressure. You can change the upper and lower limits within the allowed ranges. In addition, you can change the pressure unit.
Page 54 UltiMate 3000 Series: SD, RS, and BM Pumps Solvents Page or Left Solvents and Right Solvents Pages (depending on the pump type) Check and change the number of solvents delivered by the pump and the solvent names if necessary. ...
Page 55 UltiMate 3000 Series: SD, RS, and BM Pumps  Share Waste Bottle The check box is selected by default. Accept this setting if both pumps of the DPG-3600 are connected to the same waste container. If the check box is selected, Chromeleon assigns the two waste properties, Waste_RemainTime and Waste_WarningLimit, the same values for each pump.
Page 56: Changing The Configuration Properties
UltiMate 3000 Series: SD, RS, and BM Pumps 3.5.3.2 Changing the Configuration Properties To change the default configuration settings, reopen the configuration pages: 1. Start the Server Configuration program ( page 34). 2. Right-click the pump in the timebase and click Properties on the menu.
Page 57: Connecting A Dionex Esa Detector To The Pump
UltiMate 3000 Series: SD, RS, and BM Pumps 3.6 Connecting a Dionex ESA Detector to the Pump Note the following if you want to connect an ESA Corona or Coulochem detector to the pump:  To connect the detector to a digital I/O port on the rear panel of the pump, use the 6-pin mini-DIN signal cable (part no.
Page 58 UltiMate 3000 Series: SD, RS, and BM Pumps Page 44 Operating Instructions...
Page 59: Preparation For Operation (Startup)
UltiMate 3000 Series: SD, RS, and BM Pumps 4 Preparation for Operation (Startup) 4.1 Overview of Actions Important: The pump is filled with 2-propanol when being shipped from the factory. During initial operation of the module, make sure that the solvents used are miscible with 2-propanol.
Page 60 UltiMate 3000 Series: SD, RS, and BM Pumps 7. If you want to operate the pump with Chromeleon  Set up the pump in Chromeleon if it is not already set up ( page 31).  If you want to operate the degasser of an LPG-3400 or an SRD-3x00 Solvent Rack from Chromeleon, verify on the Devices page of the Properties dialog for the pump that the Degasser Control setting is correct (...
Page 61: General Precautions For Connecting Capillaries
Use only the capillaries shipped with the pump and original spare capillaries from Dionex.  Dionex recommends using Viper capillary connections whenever possible. When using Viper capillaries, observe the information in the instructions shipped with the capillary.  Depending on the fitting connection, also observe the following: ...
Page 62: Solvent Reservoirs
Fig. 12: Pump with Solvent Rack If the UltiMate 3000 system includes an UltiMate 3000 series autosampler, you should degas also the wash liquid on a continuous basis, for example, by using the vacuum degasser of either the LPG-3400 or an appropriate SRD-3x00 Solvent Rack.
Page 63: Connecting The Solvent Reservoirs
3. If required Verify that the end of the solvent line is cut straight and is not deformed. Cut the tubing straight if necessary. Use only the original Dionex solvent lines. 4. Place the entire assembly in the solvent reservoir.
Page 64: Iso-3100Bm
UltiMate 3000 Series: SD, RS, and BM Pumps 6. The solvent supply lines are connected to the pump at the factory.  All pumps except LPG-3400 Connect the solvent supply lines from the reservoirs and from the pump to the degassing module of an appropriate SRD-3x00 Solvent Rack.
Page 65: Setting Up The Rear Seal Wash System
UltiMate 3000 Series: SD, RS, and BM Pumps 4.4 Setting Up the Rear Seal Wash System The peristaltic pump is installed at the top left in the pump enclosure. The tubing under the peristaltic pump lever remains compressed and does not relax, thus blocking the wash solution.
Page 66 UltiMate 3000 Series: SD, RS, and BM Pumps b) Draw seal-washing liquid into a syringe at the open end of the tubing. Press the lever of the peristaltic pump to the left so that the liquid can easily pass the system.
Page 67: Connecting Drain Tubing
UltiMate 3000 Series: SD, RS, and BM Pumps 4.5 Connecting Drain Tubing To discharge liquids that might have accumulated in the interior and the seal washing liquid, the pump has a drain port at the bottom right of the instrument.
Page 68: Purging The Pump
UltiMate 3000 Series: SD, RS, and BM Pumps 4.6 Purging the Pump Purging the pump means rinsing the system for a short time at a higher flow rate. Select one of the following alternatives:  Purge the pump manually ( page 55).
Page 69: Purging The Pump Manually
UltiMate 3000 Series: SD, RS, and BM Pumps 4.6.1 Purging the Pump Manually 1. Attach a piece of silicone tubing to the outlet nozzle on the purge unit. (Silicone tubing is provided in the accessory kit of the pump.) Purge valve knob Purge outlet nozzle Fig.
Page 70 UltiMate 3000 Series: SD, RS, and BM Pumps To purge the pump from the pump display a) Select the Control menu. b) Set the channel to be purged, for example %A, to 100%. c) Start the purge cycle. To do so, set Purge to On.
Page 71: Purging The Pump By Using The Autosampler
UltiMate 3000 Series: SD, RS, and BM Pumps 4.6.2 Purging the Pump By Using the Autosampler If the UltiMate 3000 system includes a WPS-3000RS or WPS-3000SL autosampler, you can purge the pump also from Chromeleon via the membrane pump of the autosampler. In this way, you can remove small amounts of air, for example, after exchanging the solvent.
Page 72: Equilibrating The System
UltiMate 3000 Series: SD, RS, and BM Pumps 4.7 Equilibrating the System Before using the pump for sample analysis, equilibrate the UltiMate 3000 system: 1. Pump the starting solvent through the entire system until the system is free of any other liquid composition.
Page 73 UltiMate 3000 Series: SD, RS, and BM Pumps The equilibration panel shows the equilibration status of each instrument in the system. Fig. 20: Equilibration panel To equilibrate the system from the front panel menus Select and perform the operating commands and parameters on the front panel menus of the instruments.
Page 74 UltiMate 3000 Series: SD, RS, and BM Pumps Page 60 Operating Instructions...
Page 75: Operation And Maintenance
UltiMate 3000 Series: SD, RS, and BM Pumps 5 Operation and Maintenance The pump is operated with the Chromeleon Chromatography Management System. For details, see section 5.3 ( page 64). In addition, function keys and menus are available on the pump display to facilitate operation during, for example, initial installation of the pump or maintenance procedures.
Page 76: Status Screens
UltiMate 3000 Series: SD, RS, and BM Pumps 5.2 Status Screens When the self test was successful, the initial screen changes to the status screen. The pump type determines the appearance of the screen: Pump Running %A: 100.0 %B: 0.0 %C: 0.0...
Page 77 UltiMate 3000 Series: SD, RS, and BM Pumps The screen shows … When … Hold When you performed the Hold command for the pump from Chromeleon. The retention time is stopped, however, the pump continues delivering with the current settings (flow rate, solvent composition). Perform the Continue command in Chromeleon to cancel the Hold command.
Page 78: Operation With Chromeleon
UltiMate 3000 Series: SD, RS, and BM Pumps 5.3 Operation with Chromeleon Before you begin, verify that 1. The Chromeleon software is installed on the computer and the license code is entered. 2. The pump is connected to the Chromeleon computer by means of an USB connection.
Page 79: Direct Control
To open the Commands dialog box for the pump 1. Open a control panel (any panel is possible). To open a control panel, open the Chromeleon Browser and double-click a control panel in the Dionex Templates/Panels folder. 2. Connect the control panel to the timebase in which the pump is installed. On the Control menu, select Connect to Timebase, and then select the timebase on the Timebase tab.
Page 80 UltiMate 3000 Series: SD, RS, and BM Pumps The commands and parameters available in the dialog box vary, depending on the  Chromeleon version  Options selected for the pump in the Properties dialog ( page 35).  Display filter level (Normal, Advanced, or Expert) 5.
Page 81 UltiMate 3000 Series: SD, RS, and BM Pumps To open a control panel for the pump 1. On the View menu, click Default Panel Tabset or click the corresponding icon the toolbar, and then connect to the Chromeleon server. Chromeleon creates centralized control panels, called panel tabsets, for all timebases available on the Chromeleon server.
Page 82: Automated Control
UltiMate 3000 Series: SD, RS, and BM Pumps 5.3.3 Automated Control With automated control, you create a program file (PGM) for automated operation of the pump. Programs can be created automatically with the help of a software wizard or manually by editing an existing program. In addition to programs for sample analysis, you can also create programs for special purposes, for example, to automate system shutdown (...
Page 83 UltiMate 3000 Series: SD, RS, and BM Pumps 2. Change the settings in the program as desired. The easiest way is to edit a program is to do this in the Device Views ( Fig. 24). Click a device icon and change the settings on the device pages. Editing the program in the Device Views ensures correct command syntax.
Page 84: Function Keys And Menus On The Pump Display
(or Flow off), Set flow, and Purge ( table further down in this section). To show the keys, touch the position of the utmost left spot on the pump display with the Dionex menu pen. The menu pen is included in the pump shipment.
Page 85 UltiMate 3000 Series: SD, RS, and BM Pumps To … Select ... Menu Open the Main menu ( page 74). Have the pump deliver with the specified flow rate. Flow on While is pump is idle, the nominal flow rate is displayed and the flow value is flashing.
Page 86: Pump Menus
UltiMate 3000 Series: SD, RS, and BM Pumps 5.4.2 Pump Menus Fig. 27 shows an overview of the pump menus, here as an example for a DGP-3600RS. The pump type determines which menu items are available on the menus. For information about the general menu layout and structure, see page 73. For information about the commands and parameters that are available on the menus, see sections 5.4.2.2...
Page 87: General Menu Layout And Structure
UltiMate 3000 Series: SD, RS, and BM Pumps 5.4.2.1 General Menu Layout and Structure In general, the menu layout is as follows: > Control (1..4) 1. Flow 2. Flow rate 3. %A 4. %B   Select Back Fig. 28: Menu layout (here Control menu) Description Reports the menu name and the number of items on the menu list.
Page 88: Main Menu
UltiMate 3000 Series: SD, RS, and BM Pumps To … Select ... Cancel the action and restore the last value. Cancel Note: Depending on the selected option, specific keys may replace these general keys. If an error is found, one or more messages appear on the pump display. In this case, the Prev, Next, and Clear keys appear on the navigation bar.
Page 89: Control Menu
UltiMate 3000 Series: SD, RS, and BM Pumps 5.4.2.3 Control Menu On the Control menu, you can make the different settings for pump operation. To … Select ... Start the pump flow with the selected flow rate or to stop the flow.
Page 90: Diagnostics Menu
Leak detection is enabled as a standard. For more information about how operate the pump with leak detection, see page 86. Set the pressure unit. Pressure unit Restore the Dionex standard settings. Reset to factory defaults In the Reset to factory defaults? dialog box, select OK to confirm the restore or select Cancel to keep your settings.
Page 91: Information For Operating The Pump
UltiMate 3000 Series: SD, RS, and BM Pumps 5.5 Information for Operating the Pump This section provides specific information about settings and functions that should be considered for operating the pump. To learn more about ... On page … Choosing the solvents...
Page 92 Chemical reactions may also occur when using tetrachloromethan, diethyl ether, ethyl ether, di-isopropyl ether, ketones, methylbenzene, methycyclohexane, and monochlorobenzene. If you use these solvents, contact your Dionex sales representative.  If the UltiMate 3000 system includes an LPG-3400 pump or an SRD-3x00 Solvent Rack, observe also the solvent compatibility of the degasser.
Page 93 You should not recycle the solvent or use methanol from aluminum reservoirs. This may impair the performance of the seals. Important: Dionex déconseille de recycler les solvants ou employer du méthanol stocké dans des réservoirs en aluminium. Ceci peut affecter les performances des joints.
Page 94: Linking The Pump To The Autosampler
5.5.2 Linking the Pump to the Autosampler Only if the UltiMate 3000 system includes a WPS-3000 or ACC-3000 In this case, you can specify which pump is linked to the autosampler. Dionex recommends always specifying a pump because this setting ...
Page 95: Setting The Flow Rate, Flow Acceleration, And Flow Deceleration
 If the values are too high, this may or reduce the lifetime of the column. Dionex recommends that you set these parameters to values between 1/3 and factor 3 of the (column) flow rate. To set the flow rate, flow acceleration, and flow deceleration in Chromeleon 1.
Page 96: Setting The Pressure Limits
UltiMate 3000 Series: SD, RS, and BM Pumps 5.5.4 Setting the Pressure Limits The pump firmware and Chromeleon provide default values for the upper and lower pressure limits. The limits depend on the pump type. You are free to change the limits within the allowed pressure range.
Page 97: Recording The Pump Pressure
UltiMate 3000 Series: SD, RS, and BM Pumps 5.5.5 Recording the Pump Pressure On the Signals page, the check box for the pump pressure is selected by default when the pump is installed and configured in Chromeleon ( page 41). With this setting, Chromeleon generates the appropriate channel for recording the pump pressure.
Page 98: Choosing The Seal-Washing Liquid
UltiMate 3000 Series: SD, RS, and BM Pumps 5.5.6.2 Choosing the Seal-Washing Liquid Observe the precautions for the composition of the seal washing liquid:  Make sure that the liquid used for rear seal washing is miscible with the solvent. This is to avoid impairing the tightness of the pump.
Page 99: Purging The Pump
UltiMate 3000 Series: SD, RS, and BM Pumps Take the following remedial action:  Check the liquid level in the liquid reservoir of the rear seal wash system.  Replace the peristaltic tubing.  Verify that the liquid can pass the tubing properly. Replace the tubing if required.
Page 100: Detecting Liquid Leaks In The Pump
UltiMate 3000 Series: SD, RS, and BM Pumps 5.5.8 Detecting Liquid Leaks in the Pump Leak detection is enabled as a standard when the pump is shipped. When leak detection is active and the leak sensor reports a leak ...
Page 101: Adjusting The Screen Brightness Or Contrast
UltiMate 3000 Series: SD, RS, and BM Pumps 5.5.9 Adjusting the Screen Brightness or Contrast You can adjust the screen brightness or screen contrast to your requirements from Chromeleon or on the pump display. To do so, select one of the following alternatives: ...
Page 102: Vacuum Degasser (Lpg-3400 And Srd-3X00)
In addition, observe the following to ensure optimum degassing performance:  Dionex recommends filling all channels with eluent and degassing all channels (even if they are not used for the application). This will reduce the speed of the vacuum pump and reduce the degasser noise.
Page 103: Turning The Degasser On And Off
Reports whether the leak sensor in the SRD detected a leak. Tips for operating the degasser  Dionex recommends always leaving the degasser on while the pump is on.  Turning off the pump to which an SRD-3x00 Solvent Rack is connected also turns off the solvent rack.
Page 104: General Precautions For Operating An Iso-3100Bm
UltiMate 3000 Series: SD, RS, and BM Pumps 5.5.12 General Precautions for Operating an ISO-3100BM When operating an ISO-3100BM, observe the following:  When the fluid components of the pump are filled with liquid and the solvent reservoirs are located above the pump outlet during pump operation, the hydrostatic pressure in the system may cause eluent to escape when you open a fluid connection in the pump.
Page 105: Special Chromeleon Functions
Open the Commands dialog box for the pump and enter the limits for the predictive performance parameters. For a list of the commands and counters that are supported for the pump, see the Chromeleon Help. To keep the predictive performance information up-to-date, Dionex recommends the actions listed in the table. After you have …...
Page 106 UltiMate 3000 Series: SD, RS, and BM Pumps After you have … Perform the following command … Replaced the entire pump head assembly CheckValveServiceDone , PistonsChanged SealChanged , SupportRingChanged Replaced a support ring SupportRingChanged Serviced the pump (for example, annual maintenance)
Page 107: Pump Diagnostics
UltiMate 3000 Series: SD, RS, and BM Pumps 5.6.2 Pump Diagnostics To perform the tests, the diagnostics toolkit (part no. 6040.3099) is required. The kit includes all materials required for performing the tests. 1. Verify that the signal for pump pressure is enabled ( page 41). If it is not, pump diagnostics cannot be performed.
Page 108: Setting A Gradient Curve
UltiMate 3000 Series: SD, RS, and BM Pumps 5.6.3 Setting a Gradient Curve For ramp gradients and multi-step gradients, you can specify linear or non-linear (curved) gradient profiles. The gradient profile (curve) is set in the program. 1. Create a program with the Program Wizard ( page 68).
Page 109 UltiMate 3000 Series: SD, RS, and BM Pumps Liquid Level Description Waste Container WasteLevel Set the following: Value The liquid level in the waste container at the beginning of a sequence. The upper limit for the liquid level. UpperLimit If the liquid level in the waste container reaches the upper limit and - an emergency program is available in Chromeleon, the pump is stopped as defined in the program.
Page 110: Using The Digital Inputs And Outputs (Digital I/O)
5.6.6 Operational Qualification and Performance Qualification Operational Qualification and Performance Qualification allow you to check and document the performance of the HPLC system. All materials required for performing qualification and detailed instructions are available from Dionex on request. Page 96 Operating Instructions...
Page 111: Shutting Down The Pump
(50:50) before it is shut down. This will prevent salt buildup inside the unit.  If operation is interrupted for more than 5 days, Dionex recommends removing the ® Pharmed tubing from the peristaltic pump. To remove the tubing, slightly press the lever to the left, remove the tubing, and release the lever.
Page 112 UltiMate 3000 Series: SD, RS, and BM Pumps Standby Program A standby program sets the HPLC system into standby mode. The application can be reactivated very quickly afterward. The main program steps are:  The pump flow is automatically reduced at the end of the program.
Page 113: Routine And Preventive Maintenance
UltiMate 3000 Series: SD, RS, and BM Pumps 5.8 Routine and Preventive Maintenance The pump is made of high-quality components and materials to minimize maintenance requirements. All surfaces are resistant to weak acids, alkali, and organic solvents. Nevertheless, immediately wipe up all liquids spilled onto the pump surface, by using lint- free cloth or paper.
Page 114 To avoid contamination of the degasser, you should prepare fresh solvents, clean the solvent lines, and rinse the degassing channels at regular intervals ( page 157). Recommended Annually Have Dionex Service perform preventive maintenance on the pump once a year. Page 100 Operating Instructions...
Page 115 UltiMate 3000 Series: SD, RS, and BM Pumps The following maintenance kits are available for the pumps: Maintenance kit for: Part No. ISO-3100SD 6040.1950 ISO-3100BM 6042.1950 HPG-3x00SD 6040.1953 HPG-3x00RS 6040.1956 LPG-3400SD 6040.1951 LPG-3400RS 6040.1954 LPG-3400BM 6042.1951 DGP-3600SD 6040.1952 DGP-3600RS 6040.1955 DGP-3600BM 6042.1952...
Page 116 UltiMate 3000 Series: SD, RS, and BM Pumps Page 102 Operating Instructions...
Page 117: Troubleshooting
UltiMate 3000 Series: SD, RS, and BM Pumps 6 Troubleshooting 6.1 Overview The following features help you to identify and eliminate the source for problems that may occur during the operation of the pump or UltiMate 3000 system.  Status Indicators ...
Page 118: Pump Block Status Indicator
UltiMate 3000 Series: SD, RS, and BM Pumps 6.2 Pump Block Status Indicator The status indicator for the pump block (on the interior front panel above the pump head) indicates the operational status of the pump block: Operational Status The LED is dark.
Page 119: Messages On The Pump Display
The Audit Trail may provide additional information. In addition to the messages listed in the table, other messages may appear. In this case, note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem.
Page 120 ( page 121). Configuration error: Found x (where x and y are numbers) ID chips and y pump blocks. A pump block is defective. Contact Dionex Service. Degasser malfunction LPG-3400 The vacuum level monitoring function of the degasser in the pump recognized insufficient vacuum.
Page 121 Position error A position error is reported for the pump. Turn the pump off and on again by pressing the power switch on the rear of the module. If the message appears again, contact Dionex Service. Pressure buildup during A pressure of more than 0.5 MPa built up during piston and/or piston maintenance operation.
Page 122 UltiMate 3000 Series: SD, RS, and BM Pumps Error Message Remedial Action Pressure sensor malfunction The pressure transducer for the system pressure reported a pressure under 0 MPa. Verify that the cable for the system pressure transducer is properly connected to the P-Sys connector on the interior front panel.
Page 123 UltiMate 3000 Series: SD, RS, and BM Pumps Error Message Remedial Action The pump drive is still in An attempt was made to start the pump while the pump is still in maintenance position. maintenance position, for example, during piston replacement or piston seal replacement.
Page 124 Zero position not found Turn the pump off and on again by pressing the power switch on the during the last revolution. rear of the pump. If the message appears again, contact Dionex Service. Zero position not found Turn the pump off and on again by pressing the power switch on the during the last 3 revolutions.
Page 125 UltiMate 3000 Series: SD, RS, and BM Pumps When the pump is operated with Chromeleon and if communication between Chromeleon and the pump cannot be established, related messages may appear in the Chromeleon Audit Trail. Message Remedial Action HPG-3400USB-1610103 -...
Page 126: Chromeleon Diagnostics Messages
SD, RS, and BM Pumps 6.4 Chromeleon Diagnostics Messages If the pump fails a diagnostics test, perform the instructions given here and repeat the test. If the pump still fails the test, contact Dionex Service. Degasser Vacuum Test failed Probable Cause...
Page 127 UltiMate 3000 Series: SD, RS, and BM Pumps Performance Test failed Test Result Probable Cause Remedial Action The pressure/% compression Eluent may not have been Degas the eluent. was outside specification! degassed correctly. It is likely that the wrong eluent Repeat the test.
Page 128: Operating Problems
UltiMate 3000 Series: SD, RS, and BM Pumps 6.5 Operating Problems The table provides information about common operating problems that might occur with the pump or an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information, also see the manuals for the other modules of the UltiMate 3000 system.
Page 129 UltiMate 3000 Series: SD, RS, and BM Pumps Problem Probable Cause Remedial Action The degasser of an SRD-3x00 The degasser is turned off. Turn the degasser on. Solvent Rack that is A first generation SRD-3x00 Contact Dionex Service. connected to the pump does...
Page 130 UltiMate 3000 Series: SD, RS, and BM Pumps Problem Probable Cause Remedial Action High noise level, pulsation The lamp is too old. Replace the detector lamp (Cont'd) ( Detector Manual). The wavelength is wrong. Select an appropriate wavelength. The Step is too small Increase the Step (for example, 0.1 sec.)
Page 131 UltiMate 3000 Series: SD, RS, and BM Pumps Problem Probable Cause Remedial Action Peak Broadening, The inner diameter of the Change the capillary. increased dead time capillary to the detector is too large. The filter frits on the solvent Check the filter for lines are clogged.
Page 132 UltiMate 3000 Series: SD, RS, and BM Pumps Problem Probable Cause Remedial Action Poor reproducibility The autosampler, the injection Inspect the autosampler and (Cont'd) valve, or the syringe valve is not the connections on the tight. injection valve or syringe valve.
Page 133 UltiMate 3000 Series: SD, RS, and BM Pumps Problem Probable Cause Remedial Action No flow The system is leaking. Find and eliminate the leak. One or both valve cartridges are Install the cartridges in the not installed correctly (not in the...
Page 134 UltiMate 3000 Series: SD, RS, and BM Pumps Problem Probable Cause Remedial Action Poor degassing There is a leak in the capillaries Inspect the capillary and or solvent lines or there are loose solvent tube connections for connections. leakage; tighten loose fitting connections.
Page 135: Checking The Compression Values
UltiMate 3000 Series: SD, RS, and BM Pumps 6.6 Checking the Compression Values The compression value of the pump head can provide valuable information for troubleshooting. If you observe pump pulsation and/or shifts in retention times, check the compression values: ...
Page 136 UltiMate 3000 Series: SD, RS, and BM Pumps Page 122 Operating Instructions...
Page 137: Service
SD, RS, and BM Pumps 7 Service 7.1 General Notes The following sections describe all service and repair procedures for the pump that the user may perform. All other maintenance and service procedures must be performed by Dionex personnel. Observe the following precautions: ...
Page 138: Eliminating Leakage
UltiMate 3000 Series: SD, RS, and BM Pumps 7.2 Eliminating Leakage The leak sensor is installed inside the pump and reports a leak when liquid collects in the drip tray under the fluid connections. Locate the source for the leak, eliminate the cause, and dry the leak sensor.
Page 139: Rear Seal Wash System
UltiMate 3000 Series: SD, RS, and BM Pumps 7.3 Rear Seal Wash System 7.3.1 Inspecting the Rear Seal Wash System for Leakage 1. Remove the seal wash tubing from the detector. Seal wash tubing Detector of the rear seal wash...
Page 140: Replacing The Peristaltic Tubing
UltiMate 3000 Series: SD, RS, and BM Pumps 7.3.2 Replacing the Peristaltic Tubing Description Part No. ® Peristaltic tubing (= Pharmed tubing, white) from the Tubing Kit for the Rear Seal Wash System 6040.9502 1. Remove the peristaltic tubing from the peristaltic pump. Press the lever to left, remove the tubing from the pump, and release the lever.
Page 141: Replacing The Detector
UltiMate 3000 Series: SD, RS, and BM Pumps 7.3.3 Replacing the Detector Description Part No. Detector of the rear seal wash system 6040.4131 Note the information for NP applications ( page 84). 1. Remove the seal wash tubing from the detector ( Fig. 30).
Page 142: Replacing The Check Valve Cartridges
UltiMate 3000 Series: SD, RS, and BM Pumps 7.4 Replacing the Check Valve Cartridges Two check valves are installed in the pump head: inlet check valve and outlet check valve ( Fig. 34, page 130, nos. 11 and 13). Description Part No.
Page 143 UltiMate 3000 Series: SD, RS, and BM Pumps Inlet valve (nut) Inlet valve (bottom part) Fig. 33: Inlet check valve 9. Reconnect the tubing connections to the inlet and outlet check valve nuts. On the outlet check valve, tighten the tubing hand-tight, and then tighten it an additional one- quarter turn, by using a wrench.
Page 144: Pistons And Piston Seals
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5 Pistons and Piston Seals Fig. 34: Pump head, piston, and piston seals (here for a HPG-3x00) Depending on the pump version, the design of some parts may differ from the representation in the picture. However, the position of the parts is the same for all pump versions.
Page 145 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. HPG-3x00RS HPG-3x00SD Pump head, entire assembly, with: 6040.1901A 6040.1903A Piston (sapphire) 6040.0042 (= 2 pistons) Plate of the rear seal wash system ----- Piston seal (reversed phase) 6040.0304 (= 2 seals) Seal ring (rear seal wash system) Included in 6040.2208...
Page 146 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. LPG-3400RS ISO-3100SD DGP-3600RS LGP-3400SD DGP-3600SD Pump head, entire assembly, with: 6040.1902A 6040.1904A Piston (sapphire) 6040.0042 (= 2 pistons) Plate of the rear ----- seal wash system Piston seal (reversed phase) 6040.0304 (= 2 seals)
Page 147 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. LGP-3400BM DGP-3600BM ISO-3100BM Pump head, entire assembly, with: 6042.1902 Piston (sapphire) 6040.0042 (= 2 pistons) Plate of the rear seal wash system ----- Piston seal (reversed phase) Included in 6025.2012 Seal ring (rear seal wash system) Included in 6040.2208...
Page 148: Visually Inspecting The Pump For Piston Seal Leakage
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.1 Visually Inspecting the Pump for Piston Seal Leakage You can inspect the pump visually for liquid leaks from the piston seals. 1. Flush the rear seal wash system with the seal-washing liquid.
Page 149: Pump Head And Pistons
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.2 Pump Head and Pistons 7.5.2.1 Removing the Pump Head and Pistons 1. Tilt the front cover upward. 2. If necessary, purge the pump to remove toxic solvents. 3. Set the pump flow rate to 0 and wait until the system pressure is down to zero.
Page 150 UltiMate 3000 Series: SD, RS, and BM Pumps 8. Loosen the pump head screw with the Allen wrench (size 6 mm) from accessories kit of the pump. 9. Hold the pump head with one hand in its position, remove the pump head screw, and then remove the pump head toward you.
Page 151: Installing The Pistons And Pump Head
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.2.2 Installing the Pistons and Pump Head Description Part No. Pump head (entire assembly) HPG-3x00RS 6040.1901A HPG-3x00SD 6040.1903A LPG-3400RS and DGP-3600RS 6040.1902A ISO-3100SD, LPG-3400SD, and DGP-3600SD 6040.1904A ISO-3100BM, LPG-3400BM, and DGP-3600BM 6042.1902 Piston (2 pistons, sapphire) 6040.0042...
Page 152 UltiMate 3000 Series: SD, RS, and BM Pumps 4. Reconnect the fluid connections on the pump head. Observe the following:  When installing the capillary from the pump head to the purge unit, make sure that you install the capillary in the direction of solvent flow, as indicated by the arrow on the capillary.
Page 153: Replacing The Piston Seals
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.3 Replacing the Piston Seals The seals prevent solvent from leaking into the rear seal wash system or into the pump. This may cause unstable flow rates and baseline noise. Each piston has two piston seals. One seal is in the pump head ( Fig. 34, no. 7, page 130);...
Page 154: Disassembling The Pump Head And Removing The Piston Seals
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.3.1 Disassembling the Pump Head and Removing the Piston Seals 1. To remove the pump head and pistons, follow the steps in section 7.5.2.1 ( page 135). Tip: Never disassemble the pump head with bare hands. Put on a pair of clean room gloves to prevent contamination on the pump parts.
Page 155: Cleaning The Pistons
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.3.2 Cleaning the Pistons Cleaning the pistons is required only if you reinstall the pistons you removed. Verify that the pistons are clean and free of damage. Hold the rear side of the piston into the light. Dirt particles will be enlarged by the refraction of the light.
Page 156: Installing The Piston Seals And Reassembling The Pump Head
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.3.3 Installing the Piston Seals and Reassembling the Pump Head Description Part No. Piston Seal, Reversed Phase, 2 seals ( Fig. 34, page 130, nos. 3 and 7 ) 6040.0304 RS and SD pumps Included in 6025.2012...
Page 157 UltiMate 3000 Series: SD, RS, and BM Pumps 4. Push the piston seal tool into the pump head as far as it goes in. 5. Remove the tool from the pump head. The piston seal and the support ring remain in the pump head.
Page 158: Recommended Actions After Piston Seal Replacement
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.3.4 Recommended Actions after Piston Seal Replacement Observe the following recommendations when you have replaced the piston seals in the pump head: Tip: Pump performance and the seal life cycle depend directly on the actions recommended in this section.
Page 159: Replacing The Piston Seals In The Plate Of The Seal Wash System
UltiMate 3000 Series: SD, RS, and BM Pumps 7.5.3.5 Replacing the Piston Seals in the Plate of the Seal Wash System 1. To remove the pump head and pistons, follow the steps in section 7.5.2.1 ( page 135). 2. Remove the plate of the rear seal wash system.
Page 160: Mixing System
UltiMate 3000 Series: SD, RS, and BM Pumps 7.6 Mixing System RS and SD pumps except ISO-3100SD The steps described in the following sections refer to the replacement procedure for the mixers with which the pump is shipped ( page 22).
Page 161: Static Mixer
UltiMate 3000 Series: SD, RS, and BM Pumps 7.6.2 Static Mixer 7.6.2.1 Replacing the Static Mixer Description Part No. Static mixer (volume: 350 µL), for HPG-3x00SD, LPG-3400 and DGP-3600 (both SD and RS) 6040.5310 Static mixer (volume: 150 µL), for HPG-3x00RS 6040.5110...
Page 162: Checking The Static Mixer For Permeability
UltiMate 3000 Series: SD, RS, and BM Pumps 7.6.2.2 Checking the Static Mixer for Permeability Check the permeability of the static mixer at regular intervals. Tip: You can check the permeability of the inline filter also by running the Mixer Frit Test from Chromeleon (...
Page 163: Inline Filter
UltiMate 3000 Series: SD, RS, and BM Pumps 7.7 Inline Filter 7.7.1 Replacing the Inline Filter (ISO-3100) Description Part No. ISO-3100SD and ISO-3100BM Inline filter (volume: 150 µL) 6040.5110 ISO-3100SD Capillary from purge unit to inline filter 6040.3024 ISO-3100BM Included in 6042.3002...
Page 164: Inline Filter (Lpg-3400Bm, Dgp-3600Bm)
UltiMate 3000 Series: SD, RS, and BM Pumps 7.7.2 Inline Filter (LPG-3400BM, DGP-3600BM) 7.7.2.1 Replacing the Inline Filter Description Part No. Inline filter for LPG-3400BM and DGP-3600BM 6042.5014 (volume: 10 µL) Capillary from purge unit to inline filter 6042.3024 (LPG-3400BM, DGP-3600BM)
Page 165: Replacing The Filter Frit In The Inline Filter
UltiMate 3000 Series: SD, RS, and BM Pumps 7.7.2.2 Replacing the Filter Frit in the Inline Filter Description Part No. Filter frits for inline filter (LPG-3400BM and DGP-3600BM) 6268.0036 (2 frits, titanium, porosity: 2 µm) 1. Remove the inline filter as described in section 7.7.2.1.
Page 166: Checking The Inline Filter For Permeability
UltiMate 3000 Series: SD, RS, and BM Pumps 7.7.3 Checking the Inline Filter for Permeability Check the permeability of the inline filter at regular intervals. Tip: You can check the permeability of the inline filter also by running the Mixer Frit Test from Chromeleon (...
Page 167: Replacing The Purge Valve Knob
UltiMate 3000 Series: SD, RS, and BM Pumps 7.8 Replacing the Purge Valve Knob Description Part No. Purge valve knob (with integrated cap seal) 6040.2035 Replace the purge valve knob if  Leakage is observed around the valve knob when the valve is open.
Page 168: Testing The Pump For Leakage
UltiMate 3000 Series: SD, RS, and BM Pumps 7.9 Testing the Pump for Leakage After you have carried out any maintenance or repair work on the fluid system, test the pump for leakage. Tips: With a DPG-3600, you have to perform the test twice, that is, separately for each of the two pumps.
Page 169 UltiMate 3000 Series: SD, RS, and BM Pumps 5. Have the pump deliver some µL/min until the following pressure has built up: Pump Type Pressure RS pumps 75 MPa SD pumps 45 MPa BM pumps 35 MPa 6. When this pressure has been built up and when the pump delivers a master flow of 1 µL/min (ISO-3100BM: 10 µL/min), the pressure should increase or remain constant...
Page 170 UltiMate 3000 Series: SD, RS, and BM Pumps c) After the stabilization time, monitor the pressure drop. d) Tighten the connection a little more tight. The pressure will suddenly increase a little. e) Monitor if the pressure drops at the same rate as before. If the pressure drops significantly slower, the connection was leaking.
Page 171: Vacuum Degasser (Rinsing The Degassing Channels)
UltiMate 3000 Series: SD, RS, and BM Pumps 7.10 Vacuum Degasser (Rinsing the Degassing Channels) To avoid contamination of the degasser, you should prepare fresh solvents, clean the solvent lines, and rinse the degassing channels at regular intervals. Rinsing the degassing channels is especially important for the channel that degasses aqueous solvents (for reverse-phase chromatography).
Page 172: Replacing The Main Power Fuses
1. Remove the fuse cartridge, by using a small screwdriver. Fuse cartridge Fig. 53: Fuse cartridge 2. Replace the fuses. Dionex recommends always replacing both fuses. Description Part No. Fuse, 2A, slow-blow (5 x Included in the Fuses Kit, part no.
Page 173: Updating The Pump Firmware
UltiMate 3000 Series: SD, RS, and BM Pumps 7.12 Updating the Pump Firmware The pump is shipped with the most recent firmware version. The pump firmware is also included in Chromeleon. To check which firmware version is installed in the pump and which version is included in Chromeleon: ...
Page 174 If the download is not successful, the related messages appear in the Audit Trail. In this case, turn off the pump. Turn on the pump again and repeat the download as described above. If the download fails again, contact Dionex Service. Page 160...
Page 175: Pump-Specific Information
UltiMate 3000 Series: SD, RS, and BM Pumps 8 Pump-Specific Information The sections listed in the table provide a short overview of the interior components and liquid flow path through the pump. For the … Find information about the ...
Page 176: Iso-3100
UltiMate 3000 Series: SD, RS, and BM Pumps 8.1 ISO-3100 8.1.1 ISO-3100SD 8.1.1.1 Interior Components Fig. 54: Interior components (ISO-3100SD) Description Peristaltic pump Detector of the rear seal wash system Capillary guides Pump head with working cylinder and equilibration cylinder Leak sensor Inline filter (...
Page 177: Flow Schematic
UltiMate 3000 Series: SD, RS, and BM Pumps 8.1.1.2 Flow Schematic The picture illustrates the liquid flow path through the pump. Fig. 55: Flow path through the ISO-3100SD Description Part No.  page 13 SRD-3x00 Solvent Rack with analytical vacuum degasser Solvent line from analytical degasser to pump head 6030.2546...
Page 178: Operating Principle (Schematics)
UltiMate 3000 Series: SD, RS, and BM Pumps 8.1.1.3 Operating Principle (Schematics) The picture illustrates how the pump operates. Fig. 56: Operating principle of the ISO-3100SD Element Pump head with working cylinder (no. 1a) and equilibration cylinder (no. 1b) Purge unit with purge valve knob (no.
Page 179: Iso-3100Bm
UltiMate 3000 Series: SD, RS, and BM Pumps 8.1.2 ISO-3100BM 8.1.2.1 Interior Components Fig. 57: Interior components (ISO-3100BM) Description Peristaltic pump Detector of the rear seal wash system Capillary guides Pump head with working cylinder and equilibration cylinder Leak sensor Pulse damper Inline filter (...
Page 180: Flow Path
UltiMate 3000 Series: SD, RS, and BM Pumps 8.1.2.2 Flow Path The picture illustrates the liquid flow path through the pump. Fig. 58: Flow path through the ISO-3100BM Description Part No.  page 13 SRD-3x00 Solvent Rack with analytical vacuum degasser (optional) Solvent line from analytical degasser to pump head 6030.2546...
Page 181: Operating Principle (Schematics)
UltiMate 3000 Series: SD, RS, and BM Pumps 8.1.3 Operating Principle (Schematics) The picture illustrates how the pump operates. Fig. 59: Operating principle of the ISO-3100BM Element Pump head with working cylinder (no. 1a) and equilibration cylinder (no. 1b) Purge unit with purge valve knob (no.
Page 182: Lpg-3400
UltiMate 3000 Series: SD, RS, and BM Pumps 8.2 LPG-3400 8.2.1 Interior Components Fig. 60: Interior components (here LPG-3400SD/RS) Instead of the two-step mixing system, LPG-3400BM pumps have an inline filter, which is located below the pump head. Fig. 61: Inline filter (LPG-3400BM)
Page 183: Flow Path
UltiMate 3000 Series: SD, RS, and BM Pumps 8.2.2 Flow Path The picture illustrates the liquid flow path through the pump. Fig. 62: Flow path through an LPG-3400 (top: LPG-3400SD/RS, bottom: LPG-3400BM) Operating Instructions Page 169...
Page 184 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. SR-3000 Solvent Rack  page 13 Solvent lines to degasser (4 lines) 6040.2049 Analytical 4-channel vacuum degasser ---- Note: You are free to choose the direction of flow through the degasser.
Page 185: Operating Principle (Schematics)
UltiMate 3000 Series: SD, RS, and BM Pumps 8.2.3 Operating Principle (Schematics) The picture illustrates how the pump operates. Fig. 63: Operating principle of the LPG-3400 Element Inbuilt vacuum degasser Proportioning valve Pump head with working cylinder (no. 3a) and equilibration cylinder (no. 3b) Purge unit with purge valve knob (no.
Page 186: Dgp-3600
UltiMate 3000 Series: SD, RS, and BM Pumps 8.3 DGP-3600 8.3.1 Interior Components Fig. 64: Interior components (here DGP-3600SD/RS) Instead of the two-step mixing system, DGP-3600BM pumps have an inline filter, which is located below the pump head. Fig. 65: Inline filter (DGP-3600BM)
Page 187: Flow Path
UltiMate 3000 Series: SD, RS, and BM Pumps 8.3.2 Flow Path The picture illustrates the liquid flow path through the pump. Fig. 66: Flow path through a DGP-3600 (top: DGP-3600SD/RS, bottom: DGP-3600BM) Operating Instructions Page 173...
Page 188 UltiMate 3000 Series: SD, RS, and BM Pumps No. Description Part No. SRD-3x00 Solvent Rack with analytical vacuum degasser  page 13 Solvent lines from degasser to proportioning valve (3 lines) 6030.2547 3-channel proportioning valve ------ Tubing from proportioning valve to pump head (set of 2 tubes) DGP-3600RS and DGP-3600SD 6040.3023...
Page 189: Operating Principle (Schematics)
UltiMate 3000 Series: SD, RS, and BM Pumps 8.3.3 Operating Principle (Schematics) The picture illustrates how the pump operates. Fig. 67: Operating principle of the DGP-3600 Element Element Left pump Right pump Proportioning valve Proportioning valve Pump head with Pump head with working cylinder (no.
Page 190: Hpg-3200
UltiMate 3000 Series: SD, RS, and BM Pumps 8.4 HPG-3200 8.4.1 Interior Components Fig. 68: Interior components in an HPG-3200 (here HPG-3200SD) Description Peristaltic pump Detector of the rear seal wash system Capillary guides Pump head with working cylinder and equilibration cylinder...
Page 191: Flow Path
UltiMate 3000 Series: SD, RS, and BM Pumps 8.4.2 Flow Path The picture illustrates the liquid flow path through the pump. Fig. 69: Flow path through the HPG-3200 Description Part No.  page 13 SRD-3x00 Solvent Rack with analytical vacuum degasser Solvent lines from analytical degasser to pump head (set of 2 lines) 6035.2530...
Page 192: Operating Principle (Schematics)
UltiMate 3000 Series: SD, RS, and BM Pumps 8.4.3 Operating Principle (Schematics) The picture illustrates how the pump operates. Fig. 70: Operating principle of the HPG-3200 Element Element Left pump head with Purge unit with working cylinder purge valve knob...
Page 193: Hpg-3400
UltiMate 3000 Series: SD, RS, and BM Pumps 8.5 HPG-3400 8.5.1 Interior Components Fig. 71: Interior components in an HPG-3400 (here HPG-3400SD) Description Peristaltic pump Detector of the rear seal wash system Capillary guides Pump head with working cylinder and equilibration cylinder...
Page 194: Flow Path
UltiMate 3000 Series: SD, RS, and BM Pumps 8.5.2 Flow Path The picture illustrates the liquid flow path through the pump. Fig. 72: Flow path through the HPG-3400 Description Part No.  page 13 SRD-3x00 Solvent Rack with analytical vacuum degasser Solvent lines from analytical degasser to solvent selectors (set of 4 lines) 6035.2532...
Page 195: Possible Gradient Combinations And Operating Principle (Schematics)
UltiMate 3000 Series: SD, RS, and BM Pumps 8.5.3 Possible Gradient Combinations and Operating Principle (Schematics) The HPG-3400 includes a "2 from 4" solvent selector. Channels A and C are connected to the left pump head, while channels B and D are connected to right pump head. Thus, the...
Page 196 UltiMate 3000 Series: SD, RS, and BM Pumps The picture illustrates how the pump operates. Fig. 73: Operating principle of the HPG-3400 Element Solvent Selector ( page 181) Left pump head with working cylinder (no. 2a) and equilibration cylinder (no. 2b) Solvent Selector Right pump head with working cylinder (no.
Page 197: Optimizing The Pump For Specific Applications
UltiMate 3000 Series: SD, RS, and BM Pumps 9 Optimizing the Pump for Specific Applications All pump types except ISO-3100SD and BM pumps In the standard configuration, the pump has been optimized for the gradient delay volume and ripple. The gradient delay volume of an HPLC system is defined as the volume that the pump must deliver until a change in solvent composition reaches the head of the column.
Page 198 UltiMate 3000 Series: SD, RS, and BM Pumps Two-step mixing systems (SpinFlow) Two-step mixing system Mixing volume including: Part No. 100 µL Static mixer (volume: 75 µL) 6040.5100 Capillary mixer (volume: 25 µL) 200 µL Static mixer (volume: 150 µL) 6040.5110*...
Page 199 UltiMate 3000 Series: SD, RS, and BM Pumps Single-step mixing system Single-step mixing system Part No. Single-step mixing system, mixing volume: 35 µL: 6040.5000 Capillary mixer (25 µL) and Inline filter (10 µL, porosity of the filter frit: 10 µm) The components of the kit are available also as separate parts: Capillary mixer (25 µL)
Page 200 UltiMate 3000 Series: SD, RS, and BM Pumps Page 186 Operating Instructions...
Page 201: Technical Information
UltiMate 3000 Series: SD, RS, and BM Pumps 10 Technical Information Specification* ISO-3100SD LPG-3400SD DGP-3600SD HPG-3200SD HPG-3400SD Serial dual-piston pump Operating Principle Compressibility Fully automated, independent of the composition of the mobile phase compensation mL/min mL/min mL/min Flow rate rage Recommended 0.05 - 10...
Page 202 UltiMate 3000 Series: SD, RS, and BM Pumps Specification* LPG-3400RS DGP-3600RS HPG-3200RS HPG-3400RS Operating Principle Serial dual-piston pump Compressibility Fully automated, independent of the composition of the mobile phase compensation Flow rate rage mL/min mL/min Recommended 0.1 - 8 0.05 - 8 Settable 0.001 - 8...
Page 203 UltiMate 3000 Series: SD, RS, and BM Pumps Specification* LPG-3400BM DGP-3600BM ISO-3100BM Operating Principle Serial dual-piston pump Compressibility Fully automated, independent of the composition of the mobile phase compensation Flow rate rage mL/min Recommended 0.05 - 2.5 Settable 0.001 - 2.5 Flow rate accuracy ±0.5%...
Page 204 UltiMate 3000 Series: SD, RS, and BM Pumps Page 190 Operating Instructions...
Page 205: Accessories, Consumables, And Spare Parts
For information about these kits, see section 11.3 ( page 199). The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact the Dionex sales organization. Description Part No.
Page 206 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Quantity in the kit Capillary (0.13 x 250 mm (I.D. x L), SST, Viper) 6040.2325 e.g., for the connection from the TCC-3000RS (column outlet) to the DAD-3000RS, MWD-3000RS, or VWD-3400RS Capillary (0.13 x 350 mm (I.D.
Page 207 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Quantity in the kit Accessories kit of SD pumps: Solvent filter, including: Filter holder (top and bottom parts) and Included in 6268.0115 filter frit (stainless steel, porosity: 10 µm) Included in 6268.0110...
Page 208 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Quantity in the kit Plastic syringe (12 mL) Included in 6000.0010 1 syringe Tubing connector (straight, for I.D. 1.0 - 2.0 mm) Included in 6040.9502 USB cable, type A to type B, high speed USB 2.0 6035.9035...
Page 209 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Quantity in the kit Dionex menu pen 6300.0100 Fuse, 2A, slow-blow (5 x 20 mm) Included in 6030.9003 Silicone tubing (O.D. x I.D. 2.80 x 1.30 mm) Included in 6000.0010...
Page 210 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Quantity in the kit Components for UltiMate 3000 system drainage: Included in 6040.0005 Cable clips (self-adhesive) Y piece T piece Elbow Connecting tube Drain tubing Installation instructions Capillary (PEEK, 1/16", 0.25 mm I.D.) with appropriate 6251.6001...
Page 211: Optional Accessories
UltiMate 3000 Series: SD, RS, and BM Pumps 11.2 Optional Accessories Accessory Description Part No. Diagnostics Tool kit The kit includes all materials required for performing pump 6040.3099 diagnostics with Chromeleon. Piston seal For SD pumps only 6025.2011A normal phase The kit contains 2 seals (NP) and 1 support ring.
Page 212 UltiMate 3000 Series: SD, RS, and BM Pumps Accessory Description Part No. Single-step mixing system for RS and SD pumps (except ISO-3100) Mixing volume: 35 µL Kit with 6040.5000 Capillary mixer (25 µL) and inline filter (volume: 10 µL) The components of the kit are available also as separate parts: Capillary mixer (25 µL)
Page 213: Consumables And Spare Parts
SD, RS, and BM Pumps 11.3 Consumables and Spare Parts The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact the Dionex sales organization. Description Part No. Bottle cap (4 caps) for rear seal wash system and solvent reservoirs 6270.0013...
Page 214 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Capillaries, Viper, for UltiMate 3000 standard systems (single stack setup) Capillary (0.18 x 550 mm (I.D. x L), SST, Viper) 6040.2355 e.g., for the connection from the ACC-3000 to the column inlet Capillary (0.18 x 450 mm (I.D.
Page 215 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Filter frit with seals for Inline filter (10 µL) in LPG-3400BM/DGP-3600BM (frit: titanium; 2 µm) 6268.0036 Inline filter (10 µL) in the single-step mixing system (frit: titanium; 10 µm) 6268.0034...
Page 216 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Maintenance kit for DGP-3600SD—including: 6040.1952 6 solvent filters with filter frits 1.5 m silicone tubing (O.D. x I.D. 2.80 mm x 1.30 mm) ® 18 cm Pharmed tubing (O.D. x I.D. 3.2 mm x 1.6 mm) 5 tubing connectors, straight, for I.D.
Page 217 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Maintenance kit for ISO-3100SD—including: 6040.1950 1 solvent filter with filter frit 1.5 m silicone tubing (O.D. x I.D. 2.80 mm x 1.30 mm) ® 18 cm Pharmed tubing (O.D. x I.D. 3.2 mm x 1.6 mm) 3 tubing connectors, straight, for I.D.
Page 218 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Mixers, capillary mixers (RS and SD pumps except for ISO-3100SD) Capillary mixer (volume: 25 µL) to be used with: 6040.3020 - Static mixer (volume: 75 µL) in the two-step mixing system (100 µL) - Inline filter (10 µL, part no.
Page 219 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Pump head, entire assembly, for HPG-3x00RS 6040.1901A HPG-3x00SD 6040.1903A LPG-3400RS and DGP-3600RS 6040.1902A ISO-3100SD, LPG-3400SD, and DGP-3600SD 6040.1904A ISO-3100BM, LPG-3400BM, and DGP-3400BM 6042.1902 Purge valve knob 6040.2035 Rear seal wash system, detector 6040.4131...
Page 220 UltiMate 3000 Series: SD, RS, and BM Pumps Description Part No. Support ring for pistons seals in RS and SD pumps (2 support rings) 6040.0012 Support ring/piston seal kit (reversed phase) for BM pumps 6025.2012 The kit includes 2 piston seals (reversed phase) and 1 support ring.
Page 221: Reference Information
UltiMate 3000 Series: SD, RS, and BM Pumps 12 Reference Information 12.1 Chemical Resistance of PEEK While PEEK has superb chemical resistance to most organic solvents, it tends to swell when in contact with trichlormethane (CHCl3), dimethyl sulfoxide (DMSO), or tetrahydrofuran (THF).
Page 222 UltiMate 3000 Series: SD, RS, and BM Pumps Medium Concentration Temperature Maximum Resistance Duration (+ = yes; - = no) (Days) Diisopropyl ether Dimethylformamide Dioctylphthalate Dioxan Ethanol 96 (Vol.) Ethyl acetate Ethylene glycol Ferric chloride Formaldehyde Formic acid Glycerin Heptane...
Page 223 UltiMate 3000 Series: SD, RS, and BM Pumps Medium Concentration Temperature Maximum Resistance Duration (+ = yes; - = no) (Days) Sodium thiosulphate Sulphur dioxide Sulphur dioxide Sulphuric acid Sulphuric acid Sulphuric acid (dissolved) Toluene Trichlormethane (chloroform) Trichloroethylene Water Xylene...
Page 224: Solvent Miscibility
UltiMate 3000 Series: SD, RS, and BM Pumps 12.2 Solvent Miscibility Miscibility describes the ability of liquids to form homogeneous mixtures in all proportions (one-phase system). Solvent miscibility is important during elution and when changing from one solvent to another. Thus, when you prepare solvents consider the miscibility and homogeneous mixing of the single components.
Page 225: Properties Of Common Solvents
UltiMate 3000 Series: SD, RS, and BM Pumps 12.3 Properties of Common Solvents The table summarizes the properties of the most important solvents in HPLC [1, 2] UV Transmission at [nm] 20% (0.7 AU) 80% (0.1 AU) 98% (0.01 AU) <...
Page 226: Safety Information About Flammable Solvents
UltiMate 3000 Series: SD, RS, and BM Pumps 12.4 Safety Information about Flammable Solvents The following table provides an overview of safety information for flammable solvents in HPLC Boiling point (°C) Vapor pressure (hPa) Flash point (°C) Auto-ignition temperature (°C)
Page 227 UltiMate 3000 Series: SD, RS, and BM Pumps References [1] Otto-Albrecht Neumüller, Römpps Chemie-Lexikon, 8. Auflage, 1987 [2] W.E. Baker et al., Explosion Hazards and Evaluation, Elsevier Sci. Publ., 1983 [3] H. Bennett, Concise Chemical and Technical Dictionary, Edward Arnold Ed., 1986 [4] D.R.
Page 228 UltiMate 3000 Series: SD, RS, and BM Pumps Page 214 Operating Instructions...
Page 229: Appendix
UltiMate 3000 Series: SD, RS, and BM Pumps 13 Appendix 13.1 Digital I/O (Pin Assignment) The two digital I/O ports provide two digital inputs and two relay outputs that can be used to exchange digital signals with external devices. Relay 2 out...
Page 230: Solvent Rack (Pin Assignment)
UltiMate 3000 Series: SD, RS, and BM Pumps 13.2 Solvent Rack (Pin Assignment) Signal Name Signal Level Remarks Reserved Solvent Rack Error TTL_high with solvent rack errors Jumper to pin 9 Solvent Rack Leak TTL_high with Solvent Rack leaks Reserved...
Page 231: Index
UltiMate 3000 Series: SD, RS, and BM Pumps 14 Index %A(B, C, or D)..........75 Right Solvents ..........40 Signals ............41 Solvents ............40 Cleaning (Pistons) ........... 141 Accessories Clear ..............74 Optional ............197 Commands (Dialog Box)........65 Standard ............191 Commands (Display).........
Page 232 UltiMate 3000 Series: SD, RS, and BM Pumps Drainage Pump .............53 General (Dialog Box) ........35 System............53 Gradient Combinations (HPG-3400) ....181 Gradient Curves ..........94 Gradient Profile (Specify) ......... 94 Equilibration ............58 Program............58 SmartStartup Wizard........58 Hold..............63 Equilibration Program ........58 HPG-3200 ............
Page 233 UltiMate 3000 Series: SD, RS, and BM Pumps Leak ..............89 Off ..............62 Leak Detection...........86 OK ..............73 Leak Rate ............154 Operating Instructions ......... 1 Leak Sensor .........23, 86, 124 Operating Principle..........15 Leak Sensor Mode ........76, 86 DGP-3600........... 175 Leak Test HPG-3200...........
Page 234 UltiMate 3000 Series: SD, RS, and BM Pumps Solvent Reservoirs ........49 System Equilibration........58 Safety..............2 Pressure Limits ..........82 Safety Precautions ..........3 Pressure Unit .............76 Seal Wash System ........21, 83 Prev..............74 Clean Detector ..........127 Program Connect............51 Create (Wizard) ..........68 Function............
Page 235 UltiMate 3000 Series: SD, RS, and BM Pumps Solvent Reservoirs ..........48 Display Messages ........105 Connect ...........48, 49 Operating Problems ........114 General Notes ..........48 Position ...........50, 54 Solvent Selector ..........181 Unit Description Solvents (Dialog Box) ........40 Biocompatible Pumps........14 Spare Parts ............199...
Page 236 UltiMate 3000 Series: SD, RS, and BM Pumps Page 222 Operating Instructions...

Dionex UltiMate 3000 Series Operating Instructions Manual

1 Introduction

2 Overview

3 Installation

4 Preparation for Operation (Startup)

5 Operation and Maintenance

6 Troubleshooting

7 Service

8 Pump-Specific Information

9 Optimizing the Pump for Specific Applications

10 Technical Information

11 Accessories, Consumables, and Spare Parts

12 Reference Information

13 Appendix

14 Index

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