Merging Image Channels - Olympus FLUOVIEW FV300 User Manual

APPLIED OPERATIONS
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2-14-2 Merging Image Channels

For instance, when a specimen is imaged with two scans for 2-channel fluorescence
observation and 1-channel transmitted light observation, the transmitted light image can
be merged to the fluorescence images to create a 3-channel image. Note that the
display gradation of the images obtained by overlaying 3 channels may be poor than
original.
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OPERATION INSTRUCTIONS
IV . Page
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/Merger/Extraction of Image Channels
Display the image slice to start the range at the front using the <Display> button at
the top of the [Display] panel.
Click the <Set start position> button. (If the start position is not set, image No. 0
becomes the start image automatically.)
Display the image slice to end the range at the front using the <Display> button.
Click the <Set end position> button. (If the end position is not set, image No. n-1,
assuming the number of image slices is n, becomes the start image automatically.)
Open the two files to be used in creating a new image.
The image files used in overlay are subjected to the following
NOTE
restrictions.
The sizes of the images in the two image files should be identical.
The number of data bits in the two image files should be identical.
(For example, it is not possible to overlay a Fluoview Multi Tiff file with
a Single TIF(S) 8-bit file.)