Page 1 ® COULTER LH 780 System Special Procedures and Troubleshooting VIII STOP PREMIX START ALARM PRIME DRAIN RINSE CLEAR APERT APERT CONT RESET POWER ON START UP SHUT DOWN POWER OFF MultiSync LCD 1700m+ PN 773023AC (December 2010) Beckman Coulter, Inc.
Page 2 This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s computer with software authorized by Beckman Coulter.
Page 3 This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for your instrument.
Page 4 REVISION STATUS PN 773023AC...
Page 5: Table Of Contents
CONTENTS WARNINGS AND PRECAUTIONS REVISION STATUS, iii INTRODUCTION, xv ® HOW TO USE YOUR COULTER LH 780 SYSTEM HARD-COPY MANUALS, xv ABOUT THIS MANUAL, xv ONLINE HELP SYSTEM, xvi CONVENTIONS, xvi CALIBRATION, 1-1 OVERVIEW, 1-1 When to Calibrate, 1-1 PERFORMING REPRODUCIBILITY...
Page 6 CONTENTS 1.12 REVIEWING ANALYZER'S CURRENT CALIBRATION FACTORS FOR AUTOMATIC ASPIRATION MODE, 1-11 1.13 REVIEWING ANALYZER'S CURRENT CALIBRATION FACTORS FOR MANUAL ASPIRATION MODE, 1-12 CLEANING PROCEDURES, 2-1 OVERVIEW, 2-1 When to Zap and Bleach Apertures, 2-1 When to Unclog Apertures, 2-1 When to Bleach RBC Internal Electrodes, 2-1 When to Clean an Aperture...
Page 7 CONTENTS E Moisten O-Rings and Return Bath, 2-12 F Check for Leaks and Bubbles, 2-12 G Close the Compartment Door Beneath the Baths and Aperture Compartment, 2-13 H Prime the System by Cycling Whole Blood., 2-13 CLEARING CLOGGED APERTURES, 2-13 ZAPPING APERTURES, 2-14 CLEANING BLOOD SAMPLING VALVE (OUTSIDE...
Page 8 CONTENTS 2.17 ADVANCED BAR-CODE READER, 2-34 Positioning the Advanced Bar-Code Reader, 2-36 Up - Service Position , 2-36 Down - Read Position , 2-36 2.18 CLEANING VACUUM ISOLATOR CHAMBER (VIC), 2-37 Summary, 2-37 A Prepare VIC for Cleaning, 2-37 B Bleach VIC, 2-38 C Return VIC to Working Order, 2-38...
Page 9 CONTENTS REPLACING CHECK VALVES, 3-7 REPLACING CHOKES, 3-8 REPLACING FUSES, 3-8 REPLACING NEEDLE, 3-9 Summary, 3-9 Procedure, 3-9 A Prepare Instrument for Needle Replacement, 3-9 B Attach Safety Clip and Remove Needle Cartridge, 3-10 C Clean Needle Assembly Area, 3-10 D Remove Needle Tubing, 3-11 E Attach Tubing to New...
Page 10 CONTENTS H Press , 3-24 I If necessary, prime the reagent associated with the tubing., 3-24 J Run a sample. Make sure that the new valve is correctly connected and there are no leaks., 3-24 3.11 REPLACING REAGENT CONTAINERS, 3-24 Recording Information in Your Logbook, 3-25 3.12 REPLACING DILUENT WHEN USING DUAL PICK-UP...
Page 11 CONTENTS Workstation Troubleshooting, 5-1 Electronic Troubleshooting, 5-1 Indicators, 5-1 Electronic Tests, 5-2 Correcting Electronic Problems, 5-2 Pneumatic/Hydraulic Troubleshooting, 5-2 Pinched Tubing, 5-2 Plug, 5-2 Leak, 5-2 Defective Components, 5-2 Correcting Pneumatic/Hydraulic Problems, 5-2 Reagent Troubleshooting, 5-3 Correcting Reagent Problems, 5-3 DILUTER FUNCTIONS, 5-3 Alphabetically by...
Page 12 CONTENTS Forcing Mixing Bubbles into Baths, 5-23 Releasing Blood Sampling Valve (BSV), 5-23 Refilling the Reagent Reservoirs, 5-24 Dispensing Retic Stain, 5-24 Dispensing Retic Clearing Solution, 5-25 Performing Sheath Tank Check, 5-25 Freeing Solenoids, 5-25 Enabling/Disabling Access to Cycle Control, 5-26 Perform an Initial Prime of Diluter, 5-26 Monitoring Low...
Page 13 CONTENTS Icons and Traffic Lights Displayed on the Workstation with System Messages, 6-2 About, Workstation Messages, 6-2 Icons and Traffic Lights Displayed on the Workstation with Workstation Only Messages, 6-3 DILUTER ERRORS, 6-4 NON-DILUTER ERRORS, 6-5 ANALYTICAL STATION MESSAGES, 6-7 WORKSTATION MESSAGES, 6-49 INDEX, INDEX-1...
Page 14 CONTENTS PN 773023AC...
Page 15 Use the Reference manual for in-depth information about what the instrument does, the methods it uses, its specifications, and information on installation, safety and software options. The Reference manual for the LH 780 System is included in the online Help system; it is available in hard copy by request.
Page 16: Index,
INTRODUCTION ONLINE HELP SYSTEM Chapter 1, Calibration Provides procedures for reproducibility, carryover, calibration with S-CAL calibrator and calibration with whole blood. Chapter 2, Cleaning Procedures Provides procedures for cleaning parts of the system. Chapter 3, Replacement Procedures Provides procedures for replacing parts of the system. Chapter 4, Adjustment Procedures Provides procedures for adjusting parts of the system.
Page 17 After the replacement of any component that involves dilution characteristics (such as the BSV) or the primary measurements (such as the apertures) When advised to do so by your Beckman Coulter Representative. You should verify the calibration of your instrument:...
Page 18: Calibration,
CALIBRATION REVIEWING REPRODUCIBILITY RESULTS Ensure you have enough normal whole blood from a single donor for a minimum of 11 and a maximum of 31 cycles. Ensure pneumatics are on. Ensure the blood detector is enabled. Ensure the number of aspirations per tube is set to 1. Select the desired mode of operation.
Page 19: Results,
CALIBRATION PERFORMING CARRYOVER CHECK Select on the Command Center to display the Quality Assurance application. Select to display the Reproducibility window. Review the results table and statistics table on the window. Use the scroll bars to review results that do not appear on the window. Verify that the CV (Coefficient of Variation) doe not exceed the established limits.
Page 20: Check,
Note: If negative carryover values persist, and the third diluent is higher than the first two, check background. If the problem persists, an instrument problem may exist, call your Beckman Coulter Representative. Select to print the carryover results for your logbook.
Page 21: Results,
CALIBRATION ® CALIBRATING CBC PARAMETERS WITH S-CAL CALIBRATOR ® Verify calibration by cycling each level of 5C Cell control in Automatic aspiration mode. Procedure A Ensure Apertures are Clean If the instrument is shut down, and you routinely shut down the instrument for at least 30 minutes every 24 hours in LH Series Cleaner, cleaning is unnecessary.
Page 22: Procedure,
CALIBRATION ® CALIBRATING CBC PARAMETERS WITH S-CAL CALIBRATOR IMPORTANT Misleading results could occur if all calibration factors are 1.000. If all calibration factors are 1.000, specify the last valid set of calibration factors from your logbook at the Analyzer. Set the number of aspirations per tube to 11. D Set Up the CBC Calibration Information at the Workstation Note: To perform this task, you must log on with a user name that was set up as an Advanced Operator or Lab Administrator.
Page 23: Window,
IMPORTANT Misleading results could occur if you transmit calibration factors that are outside the established limits. If results are outside the limits, call your Beckman Coulter Representative. The Workstation checks the results for precision (%CV within the established limits). The Workstation flags results outside the limits with a red background.
Page 24: Clean,
CALIBRATION CALIBRATING CBC PARAMETERS WITH WHOLE BLOOD Table 1.1 Calibration Criteria Precision Acceptable Calibrate Calibrate Parameter (CV%) FAC % Diff if FAC % Diff is: if Delta Diff is: CV <= 2.5% <= 5.0% >1.25% BUT <=5.0% >0.1 BUT <= 0.4 CV <= 0.8% <= 2.0% >0.7% BUT <=2.0%...
Page 25: Needed,
CALIBRATION CHANGING CALIBRATION FACTORS FOR AUTOMATIC ASPIRATION MODE Using the whole-blood specimens collected in step 2, obtain reference values from other instruments. The following methods are suggested: WBC and RBC A single-aperture impedance cell counter such as a COULTER Z Series cell counter and the manufacturer's recommended reagents.
Page 26: Mode,
CALIBRATION CHANGING CALIBRATION FACTORS FOR MANUAL ASPIRATION MODE Press 10. Repeat steps 6 through 10 for each value you want to change. 11. Press one of the following: SYSTEM RUN To cycle samples. The next sample you cycle automatically updates the calibration factors on the Workstation. RETURN To display the CALIBRATION MENU screen.
Page 27: Information,
CALIBRATION RECEIVING AVERAGE CALIBRATION FACTORS FROM WORKSTATION 1.10 RECEIVING AVERAGE CALIBRATION FACTORS FROM WORKSTATION Go to the Analyzer screen. Press MAIN MENU Press ANALYZER FUNCTIONS Press CALIBRATION Press RECEIVE AVERAGE CALIBRATION FACTORS . Status messages appear on the Analyzer while it receives the data. Press one of the following: MAIN MENU To display the Analyzer MAIN MENU screen.
Page 28: Aspiration Mode,
CALIBRATION REVIEWING ANALYZER'S CURRENT CALIBRATION FACTORS FOR MANUAL ASPIRATION MODE Press ANALYZER FUNCTIONS Press CALIBRATION Press AUTOMATIC CALIBRATION FACTORS . The existing calibration factors appear. Press one of the following: SYSTEM RUN To cycle samples. RETURN To display the CALIBRATION MENU screen. 1.13 REVIEWING ANALYZER'S CURRENT CALIBRATION FACTORS FOR MANUAL ASPIRATION MODE Go to the Analyzer screen.
Page 29: Overview,
CLEANING PROCEDURES OVERVIEW Clean the apertures as needed. Use the following information to determine the best method for cleaning based on your system’ s status. When to Zap and Bleach Apertures If you fail to recover control values. If you observe: Decreased cell counts Increased MCV values Increased voteouts.
Page 30: Plate,
If you see blood or reagent buildup on the drip plate, clean the drip plate. Only remove and clean the BSV at the advice of a Beckman Coulter Representative. Note: It is normal for crystallized reagent to be present on the external surfaces. The presence of crystallized reagent does not indicate the need for removal and cleaning of the BSV.
Page 31: For Analyzer,
CLEANING PROCEDURES CLEANING AND REPLACING AIR FILTERS For Analyzer Loosen the screws on the back of the Analyzer. D i s k - s h a p e d r e t a i n e r Open the back of the Analyzer. Pull the air filter down over the disk-shaped retainer on the screws to remove the air...
Page 32: Bleaching Apertures,
Wash the filters in soap and water, rinse them and dry them completely. If you find a filter that is torn or shredded, discard it and replace it with a new one. Order replacement filters from your Beckman Coulter Representative. Return the clean air filters to their original location.
Page 33: Solution,
CLEANING PROCEDURES BLEACHING APERTURES N. Fill the baths with cleaning agent and drain. SHUT DOWN O. Press START UP Press Q. Prime the system by cycling whole blood. A Prepare Bleach Solution Put one part bleach and one part distilled water into a wash bottle. Use a high-quality, fragrance-free bleach (5 to 6% sodium hypochlorite - available chlorine).
Page 34: Bleach,
CLEANING PROCEDURES BLEACHING APERTURES C Use F05 and Activate Solenoid 12 to Bleach the Hgb Cuvette Go to the Numeric Keypad Press to manually activate a specific solenoid. The Numeric Keypad displays SOLENOID XXX. Press 12 to activate, then press .
Page 35: Bath,
CLEANING PROCEDURES BLEACHING APERTURES E Put Bleach Solution in RBC Bath Disconnect the bleach tubing from the check valve on the top of WBC bath and connect it to the bleach check valve on the top right of the RBC bath. Put bleach solution into the RBC bath so the solution is within 1/2 in.
Page 36: Bath,
CLEANING PROCEDURES BLEACHING APERTURES G Disconnect the Wash Bottle from WBC Bath H Leave the Bleach Solution in the Baths I Go to the Numeric Keypad DRAIN J Press K Perform F12, Extended Clear Go to the Numeric Keypad. Press to apply 5-psi pressure to the rear of the apertures for 60 seconds.
Page 37: Blood.,
CLEANING PROCEDURES CLEANING AN APERTURE BATH DRAIN Press Disconnect the wash bottle. RINSE Press 10. Ensure the baths fill with diluent and the waste chamber empties. 11. Close the aperture compartment. SHUT DOWN N. Press START UP O. Press P Prime the System by Cycling Whole Blood. CLEANING AN APERTURE BATH Summary WARNING...
Page 38: Procedure,
CLEANING PROCEDURES CLEANING AN APERTURE BATH Procedure DRAIN A Press until the Aperture Baths Drain Completely into the Waste Chamber POWER OFF B Press C Unhook Springs and Pull Out Bath Open the Diluter. Manually move the rocker bed to its backward position.
Page 39: Bath,
CLEANING PROCEDURES CLEANING AN APERTURE BATH D Use Distilled Water to Clean the Bath Moisten a lint-free tissue with distilled water. Use the moist lint-free tissue to clean the openings in the bath so they are free of salt deposits. IMPORTANT Risk of instrument damage.
Page 40: Bath,
CLEANING PROCEDURES CLEANING AN APERTURE BATH E Moisten O-Rings and Return Bath Moisten the three large O-rings with distilled water. Gently rock the bath onto the large O-rings until it snaps back to its original position. Ensure you properly aligned the bath with all three blocks to prevent leaking.
Page 41: Blood.,
CLEANING PROCEDURES CLEARING CLOGGED APERTURES Both solenoids will be active at the same time. Verify that all three lines from the bath to the vacuum isolator chamber pull a bubble-free stream. Repeat steps 5 through 7 as needed to ensure the bath is not leaking. STOP If acceptable, press three times.
Page 42: Zapping Apertures,
Check for leaks and bubbles. Close the aperture compartment. Prime the system by cycling whole blood in the aspiration mode you plan to use. If the aperture remains clogged, call your Beckman Coulter Representative. ZAPPING APERTURES Ensure pneumatics are on.
Page 43: Sections),
CLEANING PROCEDURES CLEANING BLOOD SAMPLING VALVE (OUTSIDE SECTIONS) Prime the system by cycling whole blood in Automatic aspiration mode. Run a control in CBC test mode. Verify your control results are acceptable. If they are unacceptable, bleach the apertures. CLEANING BLOOD SAMPLING VALVE (OUTSIDE SECTIONS) Procedure WARNING Possible biohazardous condition.
Page 44: Bsv,
Risk of system damage. The BSV can be scratched or misaligned during this procedure. Be careful not to scratch or rub the BSV surfaces. This is not a routine procedure and should only be done if instructed to do so by your Beckman Coulter Representative. WARNING Possible biohazardous condition.
Page 45 CLEANING PROCEDURES CLEANING BLOOD SAMPLING VALVE (REMOVE AND CLEAN INSIDE) B Remove BSV Find the air cylinder. Push back the sleeve on the air cylinder BALL and rotate it so that it does not slide back over the ball joint. Lift it off the ball joint to disconnect the air cylinder from the inner section.
Page 46 CLEANING PROCEDURES CLEANING BLOOD SAMPLING VALVE (REMOVE AND CLEAN INSIDE) Unscrew and remove the knob that holds the BSV in place. Do not try to remove the BSV tubing, you may damage the fittings. Do not pull on the sample loop or any other metal fittings attached to the instrument.
Page 47: C Clean Bsv,
CLEANING PROCEDURES CLEANING BLOOD SAMPLING VALVE (REMOVE AND CLEAN INSIDE) C Clean BSV Clean the guide post and mounting post with distilled water and dry them with a lint-free tissue. CAUTION Risk of damage to ceramic sections can occur if they come in contact with an abrasive material.
Page 48: Location,
CLEANING PROCEDURES CLEANING BLOOD SAMPLING VALVE (REMOVE AND CLEAN INSIDE) D Return BSV to its Original Location IMPORTANT Incorrect results can be reported if oil or powder gets on the BSV. Before returning each section to the mounting post, moisten the inside surfaces with distilled water. Never use your fingers to spread water over the surfaces because there could be oil or powder on your gloves.
Page 49: Bsv,
CLEANING PROCEDURES CLEANING BLOOD SAMPLING VALVE (REMOVE AND CLEAN INSIDE) 10. Screw the knob onto the mounting post until you see compression of the spring, then continue until finger BALL tight. 11. Go to the air cylinder. 12. While holding the air cylinder shaft securely, push back the sleeve and SLEEVE reattach the shaft to the ball joint on...
Page 50: Flushing System,
CLEANING PROCEDURES FLUSHING SYSTEM Verify that the BSV rotates and does not leak. Close the Diluter door. Disable the blood detector. 10. Cycle a sample of diluent in Automatic aspiration mode. 11. When the instrument completes its cycle, open the Diluter door and verify that the BSV is not leaking.
Page 51: Plate,
CLEANING PROCEDURES CLEANING BLOOD SAMPLING VALVE DRIP PLATE STOP Press twice to exit this function. The Numeric Keypad displays READY. Remove the reagent line from the distilled water container and place it in the CLEANER container. SHUT DOWN Press 10. Perform daily startup. 2.10 CLEANING BLOOD SAMPLING VALVE DRIP PLATE WARNING Possible biohazardous condition.
Page 52: Cell,
2.11 CLEARING CLOGGED FLOW CELL WARNING Risk of personal injury. If non-Beckman Coulter trained personnel attempts to remove the laser, injury could result. Do not try to remove the laser from the Diluter. All service and maintenance of the laser must be done by trained Beckman Coulter personnel.
Page 53: Chamber,
CLEANING PROCEDURES DRAINING OVERFLOW CHAMBER 2.12 DRAINING OVERFLOW CHAMBER WARNING Possible biohazardous condition. The overflow chamber can contain residual biological materials and must be handled with care. Follow your laboratories protocol for safety measures. This may include, but is not limited to, wearing protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this analyzer.
Page 54: Wipe,
CLEANING PROCEDURES CLEANING MANUAL ASPIRATION PROBE WIPE 2.13 CLEANING MANUAL ASPIRATION PROBE WIPE WARNING Possible biohazardous condition. The manual aspiration probe wipe can contain residual biological materials and must be handled with care. Avoid skin contact. Clean up spills immediately in accordance with your local regulations and acceptable laboratory procedures.
Page 55: Procedure,
CLEANING PROCEDURES BLEACHING RBC INTERNAL ELECTRODES CAUTION Risk of damage to the instrument. Possible damage can occur to the instrument if the waste chamber overflows. Use F05 to activate solenoid 15 to drain the waste chamber as needed. Do not let the waste chamber get too full.
Page 56: Bath,
CLEANING PROCEDURES BLEACHING RBC INTERNAL ELECTRODES B Put the Bleach Solution in the WBC Bath Open the Diluter door. Ensure pneumatics are on. Go to the Numeric Keypad. DRAIN Press until the aperture baths drain completely into the waste chamber. Find the bleach fitting check valve on the top right of the WBC bath.
Page 57: Prime,
CLEANING PROCEDURES BLEACHING RBC INTERNAL ELECTRODES D Perform Extended Prime IMPORTANT Risk of misleading results. Make sure the baths are full before performing this procedure. If the baths are not full, you may need to prime again because bubbles could be in the line. Go to the Numeric Keypad.
Page 58: Drain,
CLEANING PROCEDURES BLEACHING RBC INTERNAL ELECTRODES F Draw Bleach into RBC Aperture and Drain CAUTION Risk of damage to the instrument. Possible damage can occur to the instrument if the waste chamber overflows. Do not let the waste chamber get too full. Use F05 to activate solenoid 15 to drain the waste chamber as needed.
Page 59: Clear,
CLEANING PROCEDURES BLEACHING RBC INTERNAL ELECTRODES G Draw Cleaning Agent into RBC Apertures and Drain Transfer the ends of tubing connected to the fittings on the RBC bath from the beaker of bleach solution to the beaker containing cleaning agent. Use F05 to activate solenoid 11 to draw cleaning agent into RBC aperture housing.
Page 60: Belt,
CLEANING PROCEDURES CLEANING THE ROCKER BED BELT 2.15 CLEANING THE ROCKER BED BELT The instrument rocks the samples prior to aspiration in Automatic aspiration mode. When the compressor is off, the rocker bed is unlocked. It can be tilted easily. I I I V I I V I I I...
Page 61: Workstation,
1. Risk of sample misidentification. When using the handheld scanner, occasional misread errors can occur as the result of partial label scans and damaged or misapplied labels. Beckman Coulter recommends that you verify each bar-code reading to assure correct patient identification.
Page 62: It 3800),
CLEANING PROCEDURES ADVANCED BAR-CODE READER After the sample ID appears on the Analyzer. Press to accept the sample ID or STOP to reject the sample ID. Ensure the sample ID is correctly displayed at the Analyzer screen. ® Cleaning the Scan Window (Welch Allyn IT 3800) Note: The following information is reprinted from the Welch Allyn IT 3800/3900 Handheld Linear Imager User's Guide by permission of Welch Allyn, Inc.
Page 63 CLEANING PROCEDURES ADVANCED BAR-CODE READER Using two decoders enables the bar-code reader to remain in a fixed position. Because the Advanced Bar-Code Reader does not have any moving part or assemblies, it does not require routine cleaning, adjustment or calibration. Imaging Bar-Code Decoder 2-35 PN 773023AC...
Page 64: Reader,
CLEANING PROCEDURES ADVANCED BAR-CODE READER Positioning the Advanced Bar-Code Reader The Advanced Bar-Code Reader has two positions: Up - Service Position. Down – Read Position Up - Service Position Remove the Diluter center cover and pull out the Advanced Bar-Code Reader lock pin.
Page 65: Summary,
CLEANING PROCEDURES CLEANING VACUUM ISOLATOR CHAMBER (VIC) 2.18 CLEANING VACUUM ISOLATOR CHAMBER (VIC) Summary WARNING Possible biohazardous condition. The VIC can contain residual biological materials and must be handled with care. Follow your laboratories protocol for safety measures. This may include, but is not limited to, wearing protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this analyzer.
Page 66: Order,
CLEANING PROCEDURES CLEANING VACUUM ISOLATOR CHAMBER (VIC) B Bleach VIC Prepare a bleach solution. Connect the bleach bottle tubing to the VIC fitting where the U-shaped tubing was disconnected. CAUTION Risk of system damage. Operating the instrument when VIC is filled with bleach can cause VIC to overflow.
Page 67: Summary,
CLEANING PROCEDURES CLEANING WASTE CHAMBER 2.19 CLEANING WASTE CHAMBER Summary WARNING Possible biohazardous condition. The waste chamber can contain residual biological materials and must be handled with care. Follow your laboratories protocol for safety measures. This may include, but is not limited to, wearing protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this analyzer.
Page 68 CLEANING PROCEDURES CLEANING WASTE CHAMBER Tilt the rocker bed back to reach the waste chamber. Find the fitting on top of the waste chamber with an extension inside and disconnect the tubing from this fitting. Disconnect the tubing from a different fitting to vent the waste chamber.
Page 69: Order,
CLEANING PROCEDURES CLEANING THE SHEAR VALVE B Bleach Waste Chamber Prepare a bleach solution. CAUTION Risk of system damage. Possible damage can occur to the instrument if the waste chamber overflows. Do not let the waste chamber get too full. Use F05 to activate solenoid 15 to drain the waste chamber as needed.
Page 70 CLEANING PROCEDURES CLEANING THE SHEAR VALVE Open the Diluter lower front door. Locate the Shear Valve labeled VL93 / 128. Diluter lower cover removed Clip Drip tray VL93/128 Remove the clip and drip tray. 2-42 PN 773023AC...
Page 71 CLEANING PROCEDURES CLEANING THE SHEAR VALVE Place lint free tissue around the outside and underneath of the Shear Valve. Rinse the outside surfaces of the Shear Valve with warm tap water. Use a cotton tip applicator to clean Shear around the middle of the valve on valve both sides.
Page 72: Plate,
CLEANING PROCEDURES CLEANING THE STRIPPER PLATE Ensure the pneumatic pressures and vacuum are at a satisfactory level. From the Diluter Keypad, press to move the valve. While the valve is moving, re-wet the surfaces again with distilled water. Clean any excess moisture with lint free tissue.
Page 73 CLEANING PROCEDURES CLEANING THE STRIPPER PLATE Go to the Numeric Keypad. P O W E R O F F Press * S Y S T E M R U N * 0 4 / 3 0 / 9 6 1 2 : 3 0 : 1 8 S T A T U S : R E A D Y A L E R T : T E S T M O D E...
Page 74 CLEANING PROCEDURES CLEANING THE STRIPPER PLATE 2-46 PN 773023AC...
Page 75: Replacement Procedures,
REPLACEMENT PROCEDURES REPLACING AIR FILTERS Procedure CAUTION Risk of damage. A dirty air filter can cause overheating. Check the filters and clean as needed. Go to the Numeric Keypad. POWER OFF Press Turn off the main breaker on the Power Supply. WARNING Risk of personal injury.
Page 76: Bath,
Wash the filters in soap and water, rinse them and dry them completely. If you find a filter that is torn or shredded, discard it and replace it with a new one. Order replacement filters from your Beckman Coulter Representative. Return the clean air filters to their original location.
Page 77: Procedure,
REPLACEMENT PROCEDURES REPLACING AN APERTURE BATH H. Replace the aperture bath and reconnect it. Reconnect the primary power cord. Turn on the main breaker on the Power Supply. K. Check for leaks and air bubbles. Close the bath compartment. START UP M.
Page 78: Instrument,
REPLACEMENT PROCEDURES REPLACING AN APERTURE BATH E Unhook the Bath Springs and Gently Pull the Bath Out of the Instrument Open the bath compartment. Manually move the rocker bed to its backward position Unhook the spring attached to each side of the bath. Disconnect the electrode cable where it attaches to the Diluter panel.
Page 79: Cord,
REPLACEMENT PROCEDURES REPLACING AN APERTURE BATH H Replace the Aperture Bath and Reconnect it Moisten the three large O-rings with distilled water. Using both hands, gently rock the bath onto the large O-rings until it snaps back to its original position. Ensure you properly aligned the bath with all three blocks to prevent leaking.
Page 80: Compartment,
REPLACEMENT PROCEDURES REPLACING CASSETTE LABELS L Close the Bath Compartment START UP M Press N Run a Control in CBC Test Mode REPLACING CASSETTE LABELS Replace worn or damaged cassette labels as needed. Remove the old label C a s s e t t e a l i g n m e n t completely.
Page 81: Clip,
REPLACEMENT PROCEDURES REPLACING CASSETTE SPRING CLIP REPLACING CASSETTE SPRING CLIP Replace the spring clip if the fingers of the spring clip become flat and the tubes are not held securely in the cassette. Grasp the spring clip in the middle of the cassette and pull it out.
Page 82: Replacing Chokes,
Return the fuse and its cap by inserting the fuse and turning the cap 1/4 turn while pushing in. CAUTION Risk of system damage if you attempt to use the power supply when a fuse continually fails. If a fuse fails shortly after replacement, power off and call your Beckman Coulter Representative. PN 773023AC...
Page 83: Reconnect The Primary Power Cord
REPLACEMENT PROCEDURES REPLACING NEEDLE Reconnect the primary power cord. 10. Turn on the Power Supply main breaker. 11. Go to the Numeric Keypad. POWER ON 12. Press REPLACING NEEDLE Summary WARNING Possible biohazardous condition. Cotton swabs and components used in this area must be handled with care.
Page 84: Cartridge,
REPLACEMENT PROCEDURES REPLACING NEEDLE B Attach Safety Clip and Remove Needle Cartridge WARNING Skin puncture from the needle, which might contain biohazardous material, can occur. Handle the needle cartridge with extreme care. Always use the safety clip to remove and install the needle cartridge. This protects you from possible needle puncture Without squeezing the safety clip, fit the right edge of the safety clip into...
Page 85: Tubing,
REPLACEMENT PROCEDURES REPLACING NEEDLE D Remove Needle Tubing If necessary, label the tubing before removing it. WARNING Possible biohazardous condition. Pulling or cutting the tubing can damage the tubing causing it to leak. Do not pull or cut the tubing. Detach the black tubing from the Instrument.
Page 86: Needle,
REPLACEMENT PROCEDURES REPLACING NEEDLE E Attach Tubing to New Needle A safety clip comes attached to the new needle cartridge. If safety clip is missing, attach one before proceeding. Attach the black tubing's white connector to the white fitting on QD 26.
Page 87: Turn On The Main Breaker On The Power Supply
REPLACEMENT PROCEDURES REPLACING APERTURE O-RINGS F Return Needle to Its Original Location Slide in the needle cartridge until it clicks into place. Slightly squeeze the safety clip and move it a little to the right to separate the right side of the clip from the needle assembly.
Page 88: Check For Leaks And Air Bubbles
REPLACEMENT PROCEDURES REPLACING APERTURE O-RINGS D. If replacing the O-rings on the RBC bath, disconnect the sweep-flow tubing. Unscrew the white fitting beneath the housing. IMPORTANT Incorrect results can occur if aperture blocks are mismatched. Remove only one aperture block at a time to avoid mismatching the apertures. Pull the block out.
Page 89 REPLACEMENT PROCEDURES REPLACING APERTURE O-RINGS Open the Diluter. Manually move the rocker bed to its backward position. Use a hemostat to unhook the spring attached to each side of the bath. IMPORTANT Risk of incorrect control results. If you move the external electrode attached to the bath you can create a carryover problem.
Page 90: Tubing,
REPLACEMENT PROCEDURES REPLACING APERTURE O-RINGS D If Replacing the O-Rings on the RBC Bath, Disconnect the Sweep-flow Tubing E Unscrew the White Fitting Beneath the Housing IMPORTANT Incorrect results can occur if aperture blocks are mismatched. Remove only one aperture block at a time to avoid mismatching the apertures.
Page 91: O-Rings,
REPLACEMENT PROCEDURES REPLACING APERTURE O-RINGS G Replace Defective O-Rings Remove and discard the defective O-rings. Fit the new O-rings onto the block. H Return Aperture Block to Its Original Location Rinse the rim of the aperture block with distilled water and dry it. Align the aperture block so the beveled edge is at the top left corner, and put it back into its housing.
Page 92: Blocks,
REPLACEMENT PROCEDURES REPLACING APERTURE O-RINGS I Repeat Steps D through H for the Remaining Aperture Blocks J Return the Bath and Reconnect It Moisten the three large O-rings with distilled water. Gently rock the bath onto the large O-rings until it snaps back to its original position.
Page 93: Compartment,
REPLACEMENT PROCEDURES REPLACING APERTURE O-RINGS Both solenoids will be active at the same time. Verify that all three lines from the bath to the vacuum isolator chamber pull a bubble-free stream. Repeat steps 5 through 7 as needed to ensure the bath is not leaking. STOP If acceptable, press three times.
Page 94: Summary,
REPLACEMENT PROCEDURES REPLACING PINCH VALVES Waste Pickup Tube Replacement: POWER OFF Press Cut tubing above pickup tube to be discarded with tubing cutters. There may be a small splash of bleach solution when the tubing is cut. Disinfect pickup tube by placing in a container with 10% bleach dilution for 15 minutes. CAUTION Do not use regular scissors as the tubing will not be cut properly.
Page 95: Procedure,
REPLACEMENT PROCEDURES REPLACING PINCH VALVES Remove the pinch valve tubing and pinch valve. Install the tubing and new pinch valve. G. Check the drawing you made in step D. Make sure the tubing is properly routed. START UP H. Press If necessary, prime the reagent associated with the tubing.
Page 96: Valve,
REPLACEMENT PROCEDURES REPLACING PINCH VALVES E Remove the Pinch Valve Tubing and Pinch Valve Disconnect the tubing that goes through the pinch valve at the fitting closest to the pinch valve. Attach a red shipping clip to hold open the valve.
Page 97: Valve,
REPLACEMENT PROCEDURES REPLACING PINCH VALVES F Install the Tubing and New Pinch Valve Use a red shipping clip to hold open the valve. Thread the I-beam tubing through the I-BEAM TUBING valve. Remove the red shipping clip. TRANSIT CLIP Line up the valve to start the 1/4-turn twist based on where you want the valve to end.
Page 98: Routed.,
Dispose of these items in accordance with your laboratory procedures. Note: If you have Dual Diluent Pick-up Tubes installed, Beckman Coulter, Inc. (BCI) recommends that you use diluent containers with the same lot number.
Page 99: Logbook,
Dispose of these items in accordance with your laboratory procedures. Note: If you have Dual Diluent Pick-up Tubes installed, Beckman Coulter, Inc. (BCI) recommends that you use diluent containers with the same lot number.
Page 100: Tubing,
Incorrect results can occur if you change the length of tubing. If tubing tears or becomes damaged, replace it with a new piece of tubing of the same type and length. If you need tubing, call your Beckman Coulter Representative. 3-26...
Page 101: Container,
REPLACEMENT PROCEDURES REPLACING WASTE CONTAINER Carefully push the tubing straight onto the fitting. Ensure it is securely in place. 3.14 REPLACING WASTE CONTAINER WARNING Possible biohazardous condition. The contents of the old waste container and its associated tubing can include residual biological material and must be handled with care. Check the tubing connection and container location periodically.
Page 102 REPLACEMENT PROCEDURES REPLACING WASTE CONTAINER 3-28 PN 773023AC...
Page 103: Mode,
ADJUSTMENT PROCEDURES CHANGING TEST MODE Go to the Command Center. to select the default test type for sample processing. Run samples for CBC, Diff and Retic. Run samples for CBC and Diff. Run samples for CBC only. Run samples for CBC and Retic. Run samples for Retic only.
Page 104: Voltage,
. The message Performing HGB Lamp Adjust appears on the HGB LAMP ADJUST Analyzer. The message Adjusting HGB appears on the Keypad display. (If the voltage appears out of range, call your Beckman Coulter Representative. Press SYSTEM RUN Cycle three (3) samples of diluent in CBC Manual aspiration mode. Ignore all results, including Hgb “..”...
Page 105: Information,
ADJUSTMENT PROCEDURES SETTING UP BAR-CODE CONFIGURATION Saving/Restoring Configuration Information Saving Configuration Information Select on the Command Center to display the System Setup window. Select to save system setup configuration information. Ensure Save is selected, and then select Select either or both of the following operations and then select Select This To Do This Save Registry...
Page 106: Database,
ADJUSTMENT PROCEDURES ENABLING/DISABLING BLOOD DETECTOR A window appears with the message, "the system will be restarted to reinitialize with the new settings". Select OK. The system shuts down windows. Another window appears with the message, " It is now safe to turn off your computer." 10.
Page 107: Time,
ADJUSTMENT PROCEDURES SETTING UP ANALYZER DATE AND TIME Press one of the following: MAIN MENU To display the Analyzer MAIN MENU screen. SYSTEM RUN To cycle samples. RETURN To display the ANALYZER FUNCTIONS screen. SETTING UP ANALYZER DATE AND TIME Go to the Analyzer screen.
Page 108: Detector,
ADJUSTMENT PROCEDURES ADJUSTING TUBE DETECTOR 4.10 ADJUSTING TUBE DETECTOR If your system produces multiple aspiration alarms when using Automatic aspiration mode, you may need to adjust the tube detector. Place a cassette of empty, stoppered tubes on the rocker bed. Ensure the pneumatics are on.
Page 109 Counterclockwise moves the sensor to the left. Verify that the needle centers CENTERED CORRECTLY directly on the tube stoppers. If the detector fails to hold the adjustment, call your Beckman Coulter Representative. OUT OF ALIGNMENT PN 773023AC...
Page 110: Scanner,
View below and print the bar-codes used to configure additional options such as Keyboard Language Code Settings, Reset to Beckman Coulter, Inc. Defaults, Code 39, Interleaved 2-of-5, Codabar, and reagent container labels. When you hear two beeps, the scanner has accepted the configuration information.
Page 111: Settings,
ADJUSTMENT PROCEDURES CONFIGURING HANDHELD SCANNER Keyboard Language Code Settings Scan This Bar-code For This Result Configure scanner to English keyboard characters (default) Configure scanner to French keyboard characters Configure scanner to German keyboard characters Configure scanner to Italian keyboard characters Configure scanner to Spanish keyboard characters Configure scanner to Japanese keyboard characters Configure scanner to read reagent container labels.
Page 112: Codabar,
ADJUSTMENT PROCEDURES CONFIGURING HANDHELD SCANNER Interleaved 2-of-5 Scan This Bar-code For This Result Enable (AIM) Interleaved 2-of-5 checksum. Disable (AIM) Interleaved 2-of-5 checksum. Enable (AIM) Interleaved 2-of-5 checksum and transmit. Enable Coulter Corporation Interleaved 2-of-5 checksum. Codabar Scan This Bar-code For This Result Disable Codabar checksum (disable all Codabar checksum techniques).
Page 113: Troubleshooting,
If your Workstation is connected to a network, some messages may be caused by your network configuration. If you have a local network administrator or a Windows 2000 Administrator, contact your administrator prior to calling your Beckman Coulter Representative. Your administrator may be able to resolve the problem.
Page 114: Electronic Tests,
Correcting Electronic Problems Although you may be able to correct minor problems, such as loose cables, most electronic problems require the assistance of your Beckman Coulter Representative. Pneumatic/Hydraulic Troubleshooting Most pneumatic/hydraulic problems are detected by observing the Diluter section in operation.
Page 115: Reagent Troubleshooting,
You can correct most reagent problems by changing the container of reagent and priming the instrument with the new reagent. DILUTER FUNCTIONS Use the following Diluter functions for troubleshooting purposes or at the advice of your Beckman Coulter Representative. Alphabetically by Function Activate and Restore Solenoid Activate and Keep Solenoid Active...
Page 116: Press
TROUBLESHOOTING DILUTER FUNCTIONS Priming Retic Clearing Solution Priming Retic Staining Solution Priming Sweep Flow Probe Wipe Test Purge Mode RBC and WBC Mix Release BSV Reservoir Refill Retic Stain Retic Clear Sheath Tank Diagnostic Solenoids Free System Flush Tube Advance Wait Enable/Disable Zap Apertures Drain the Overflow Chamber...
Page 117: Active,
TROUBLESHOOTING DILUTER FUNCTIONS Press16 to activate, then press . The Numeric Keypad displays SOLENOID 16, while processing your request. STOP Press: 2 times to return the solenoid to its original state if no other solenoid was selected. The Numeric Keypad displays READY. Drain the Waste Chamber Go to the Numeric Keypad.
Page 118: Tank,
If the baths and the vacuum overflow tank drain completely, Press to resume normal operation. If the baths and the vacuum overflow tank do not drain completely, or if the problem recurs, call your Beckman Coulter Representative. Verifying Backwash Tank Operation Go to the Numeric Keypad. Press...
Page 119: Cleaning Apertures,
TROUBLESHOOTING DILUTER FUNCTIONS F27 - Dispensing CBC Lytic Reagent Go to the Numeric Keypad. Press to dispense 1.04 mL CBC lytic reagent into the WBC bath. Note: This function is intended for troubleshooting, not for priming CBC lytic reagent. The Numeric Keypad displays CBC LYSE while the instrument performs this function. When the function completes processing, the Numeric Keypad displays FUNCTION = 27.
Page 120 Perform steps 6 through 9 up to three more times. If you do not get a result below 500, cycle a new vial of primer. If you still do not get a result below 500, call your Beckman Coulter Representative. 13. At the Numeric Keypad, press to reactivate the function for the latex control.
Page 121: Control—Diff,
TROUBLESHOOTING DILUTER FUNCTIONS 14. Gently mix the latex control according to the directions in the package insert. 15. Immerse the aspirator tip in the latex control vial. The instrument automatically aspirates the control. The Numeric Keypad displays RETIC+DIFF--LATEX while the instrument performs this function.
Page 122: Control—Retic,
If you do not get a result below 500, cycle a new vial of primer. If you still do not get a result below 500, call your Beckman Coulter Representative. 13. At the Numeric Keypad, press to reactivate the function for the latex control. The Numeric Keypad displays PRESS MANUAL OR CLEAR APERTURE.
Page 123 Perform steps 6 through 9 up to three more times. If you do not get a result below 500, cycle a new vial of primer. If you still do not get a result below 500, call your Beckman Coulter Representative. 13. At the Numeric Keypad, press to reactivate the function for the latex control.
Page 124: Volume,
TROUBLESHOOTING DILUTER FUNCTIONS F23 - Dispensing Diff Lytic Reagent Low Volume Go to the Numeric Keypad. Press to dispense Diff lytic reagent into the mixing chamber. The Numeric Keypad displays DIFF PAK LYSE LOW VOLUME while the instrument performs this function. When the function completes processing, the Numeric Keypad displays FUNCTION = 23.
Page 125: Volume,
TROUBLESHOOTING DILUTER FUNCTIONS displays FUNCTION = 21. Press to perform this function again. STOP Press to exit this function. The Numeric Keypad displays READY. F22 - Dispensing Diff Preservative High Volume Go to the Numeric Keypad. Press to dispense Diff preservative into the mixing chamber. The Numeric Keypad displays DIFF PRESERVE PAK HV DISPENSE while the instrument performs this function.
Page 126: Ensure The Pneumatics Are On
TROUBLESHOOTING DILUTER FUNCTIONS Dispensing Diluent into RBC Bath Go to the Numeric Keypad. DRAIN Ensure the pneumatics are on and press . The Numeric Keypad displays DRAIN during this processing. READY appears upon completion. Press to activate the RBC dispenser to deliver diluent into the RBC bath.
Page 127: Prime,
TROUBLESHOOTING DILUTER FUNCTIONS Performing Extended Prime IMPORTANT Risk of misleading results. Make sure the baths are full before performing this procedure. If the baths are not full, you may need to prime again because bubbles could be in the line. Go to the Numeric Keypad.
Page 128 Risk of system damage. This function places stress on the flow cell. Excessive use of this function can damage your instrument. Use this procedure only if other functions fail to remove the clog and never more than specifically instructed to do so by your Beckman Coulter product information or Beckman Coulter Representative.
Page 129: Test,
Risk of system damage. This function places greater stress on the flow cell than do other functions, and it may damage your instrument. Use this procedure only if the other functions fail to remove the clog and never more than once unless specifically instructed to do so by your Beckman Coulter Representative.
Page 130: Reagent,
The Numeric Keypad displays KEYBOARD TEST. Press any key on the Numeric Keypad. The Numeric Keypad displays the name of the key pressed. If the key fails to appear on the keypad, contact your Beckman Coulter Representative. STOP Press twice to stop the keypad test.
Page 131: Reagent,
TROUBLESHOOTING DILUTER FUNCTIONS Press to prime cleaning agent lines. The Numeric Keypad displays PRIME CLEANER while the instrument performs this function. When the function completes processing, the Numeric Keypad displays FUNCTION = 17. Press to perform this function again. STOP Press to exit this function.
Page 132: Priming Diluent,
TROUBLESHOOTING DILUTER FUNCTIONS Note: This function takes approximately 2 minutes. When the function completes processing, the Numeric Keypad displays FUNCTION = 15. Press to perform this function again. STOP Press to exit this function. The Numeric Keypad displays READY. Priming Diluent Go to the Numeric Keypad.
Page 133: Solution,
TROUBLESHOOTING DILUTER FUNCTIONS When the function completes processing, the Numeric Keypad displays FUNCTION = 19. Press to perform this function again. STOP Press to exit this function. The Numeric Keypad displays READY. Priming Retic Stain Solution Go to the Numeric Keypad. Press to prime the Retic stain lines.
Page 134: Test,
TROUBLESHOOTING DILUTER FUNCTIONS STOP Press to exit this function. The Numeric Keypad displays READY. Performing Probe Wipe Test Go to the Numeric Keypad. Press and observe that the truck moves up the full length of the probe, then down to the rest position, then moves halfway up, and finally returns to rest. During the test the Numeric Keypad displays CHECKING PROBE WIPE, and displays FUNCTION = 28, when completed.
Page 135: Baths,
TROUBLESHOOTING DILUTER FUNCTIONS When the function completes processing, the Numeric Keypad displays FUNCTION = 13. Press to perform this function again. STOP Press to exit this function. The Numeric Keypad displays READY. Forcing Mixing Bubbles into Baths Go to the Numeric Keypad. Press to force mixing bubbles into the RBC and WBC baths.
Page 136: Dispensing Retic Stain,
TROUBLESHOOTING DILUTER FUNCTIONS Press to perform this function again. STOP Press twice to exit this function. The Numeric Keypad displays READY. Refilling the Reagent Reservoirs Go to the Numeric Keypad. Press to prime the reagent reservoirs. The Numeric Keypad displays REFILLING ... (whatever reagent is low) while the instrument performs this function.
Page 137: Solution,
TROUBLESHOOTING DILUTER FUNCTIONS Dispensing Retic Clearing Solution Go to the Numeric Keypad. Press to dispense Retic clearing solution into the mixing chamber. The Numeric Keypad displays RETIC CLEAR while the instrument performs this function. When the function completes processing, the Numeric Keypad displays FUNCTION = 26.
Page 138: Control,
TROUBLESHOOTING DILUTER FUNCTIONS the function completes processing, the Numeric Keypad displays FUNCTION = 95. Press to perform this function again. STOP Press to exit this function. The Numeric Keypad displays READY. Enabling/Disabling Access to Cycle Control Go to the Numeric Keypad. Press to enable or disable access to cycle control.
Page 139: Place A Cassette Containing Tubes In Each Cassette Position On The Rocker Bed To The Right Of The Tube Detector
TROUBLESHOOTING DILUTER FUNCTIONS Monitoring Low Vacuum Go to the Numeric Keypad. Press to monitor low vacuum. The Numeric Keypad displays X.XXXX, where X.XXXX is the current vacuum level. The vacuum level may appear to change slightly while you view it on the Numeric Keypad. This is normal. Use the vacuum regulator to adjust the level to 6 ±0.01 (5.99 to 6.01) inches Hg.
Page 140 TROUBLESHOOTING DILUTER FUNCTIONS Apply pressure behind the baths. Drain and rinse the baths four times with cleaning agent. Draw cleaning agent behind the apertures. Zap the apertures 10 times to remove protein or debris from the apertures. The Numeric Keypad displays ZAP APERTURES while the instrument performs this function.
Page 141 TROUBLESHOOTING SOLENOID FUNCTIONS SOLENOID FUNCTIONS Table 5.1 Solenoid Functions Solenoid Function Components Activated Rinse Needle Lee Valve Backwash Probe Lee Valve CBC Lyse Pump PM7, VL3 Hgb Drain Valve VL4A, VL4B, VL4C Backwash Pressure Backwash Tank (VC23) Hgb and Waste Drain WBC Dispense PM3, VL7 RBC Dispense...
Page 142 TROUBLESHOOTING SOLENOID FUNCTIONS Table 5.1 Solenoid Functions (Continued) Belt Advance Needle Bellow Up Not used Vacuum Overflow Dump VL39, VC22 Stop Vacuum VL40 Reverse Flush VL41 Rinse - Diff Lee Valve Diff Lyse (PAK Lyse) PM4, CVL43 Sample Disable - Retics VL44 Diff Preserve (PAK Preserve) PM1, VL45...
Page 143 TROUBLESHOOTING SOLENOID FUNCTIONS Table 5.1 Solenoid Functions (Continued) RF On Logic Signal to Trigger RF On Mixer - Diff Logic Signal to Trigger Diff Mixing Motor Count - Diff/Retics Logic Signal to Trigger Diff/Retics Count Rinse Retic Chamber Lee Valve Sample Pressure - Retics VL76 Diluent Sense...
Page 144: Go To The Analyzer Screen
SYSTEM RUN Review the results at the Workstation. If results are unacceptable, rerun the background test. If the results are unacceptable on the second try, call your Beckman Coulter Representative. If background test results are acceptable, begin running controls or press...
Page 145: Test,
Workstation. Review the results at the Workstation. If results are unacceptable, rerun the precision test. If the results are unacceptable on the second try, call your Beckman Coulter Representative. If precision test results are acceptable, press one of the following: MAIN MENU To display the Analyzer MAIN MENU screen.
Page 146 TROUBLESHOOTING CHECKING THE NEEDLE Press one of the following: MAIN MENU To display the Analyzer MAIN MENU screen. SYSTEM RUN To cycle samples. RETURN To display the ANALYZER FUNCTIONS screen. CHECKING THE NEEDLE Cycle two samples: One in Automatic aspiration mode (to check the sample flow to the BSV). While cycling the sample, blood passes through the BSV.
Page 147: Card,
BLOOD DETECT: ON VIII You should only open the Analyzer cover at the instruction of your Beckman Beckman Coulter Representative. Locate the circuit card you want to reseat. A P S 1 A M C C O M M I N T E R F A C E...
Page 148 TROUBLESHOOTING RESEATING AN ANALYZER CARD Loosen the thumbscrews on the upper and lower front edge of the circuit card. B . E x t e n d t a b s A . U n f a s t e n t h u m b s c r e w s C .
Page 149 TROUBLESHOOTING RESEATING AN ANALYZER CARD 13. Close the Analyzer cover. 14. Reconnect the primary power cord. 15. Turn on the main breaker on the Power Supply. POWER ON 16. Press 5-37 PN 773023AC...
Page 150 TROUBLESHOOTING ANALYZER RESET BUTTON 5.11 ANALYZER RESET BUTTON Reset button Press this button to reset the Analyzer. Resetting the Analyzer: Resets the electronic and pneumatic subsystems. Causes the baths to drain and rinse and a backwash to occur. Performs a memory test and senses the reagent and waste levels. If used when the unit is not cycling, data from the last sample in the Analyzer memory is erased.
Page 151: Logbook,
TROUBLESHOOTING WORKING WITH ELECTRONIC HISTORY LOGBOOKS Each log presents identification information, such as the date and time a message was logged, and system messages. You can access detailed descriptions and corrective actions for system messages by selecting and then selecting an entry in the log. The most current message appears at the top of the window.
Page 152: Cover,
TROUBLESHOOTING OPENING THE DILUTER LOWER COVER 5.13 OPENING THE DILUTER LOWER COVER Place your hands on each side of the lower cover and pull it toward you. Place your hand on top of the lower cover and pull it toward you, lowering it to a resting position.
Page 153: Autostop ,
TROUBLESHOOTING CLOSING THE DILUTER LOWER COVER 5.14 CLOSING THE DILUTER LOWER COVER Lift the cover upward. Push the cover back toward the instrument. 5.15 AUTOSTOP The LH Workstation software allows you to configure the system to automatically halt under various conditions. By configuring the system to halt, it avoids analyzing samples during a fault condition that could affect parameter results or slide preparation.
Page 154: Autostop Options,
TROUBLESHOOTING COMMUNICATION HAS BEEN LOST BETWEEN THE ANALYTICAL STATION AND WORKSTATION AutoStop Options Category Definition Configuration Options Control A control failure has occurred. Detail is Disabled or Enabled for all. provided in Control AutoStop On. Applies to both traditional Controls and Extended QC.
Page 155: Lines,
TROUBLESHOOTING FLUSHING LOW VACUUM LINES optimize the processing speed of the system. At a minimum, the Workstation should be shutdown weekly. When the Shutdown Computer dialog box displays “It is now safe to restart your computer”, turn off the computer. Allow it to remain off for a minute and then turn it back on. Depending on how much optimization needs to occur and how long it has been since the system was last shutdown, it may take as much as 60 minutes or as little as five.
Page 156 TROUBLESHOOTING FLUSHING LOW VACUUM LINES Remove the Diluter right-side panel by lifting up and out on the panel. Open the lower right side door as shown. Remove the clear plastic protective covering by unscrewing the white plastic screw. 5-44 PN 773023AC...
Page 157 TROUBLESHOOTING FLUSHING LOW VACUUM LINES Locate the tubing connected to the Count Vacuum. Remove the yellow and black-striped tubing from the reducer fitting. Fill the container with no more than 22 cc of distilled water and place the tubing into the container. NOTE: The vacuum will draw the water out of the container.
Page 158 TROUBLESHOOTING FLUSHING LOW VACUUM LINES Let the instrument set idle for a minimum of 10 minutes to allow the tubing to dry, and then reconnect the tubing back into the fitting. Replace the plastic protective covering and secure it with the white plastic screw.
Page 159 TROUBLESHOOTING FLUSHING LOW VACUUM LINES 12. Open the left-side Diluter cover by lifting up and out on the cover. 13. Locate VC22, the Vacuum Overflow Tank, in the bottom section of this area. 14. Disconnect the float sensor connector labeled SEN1 J13, located above the tank.
Page 160 TROUBLESHOOTING FLUSHING LOW VACUUM LINES 16. Press Alarm Reset on the Analyzer keypad. The keypad display will change to a Ready status. 17. Reconnect SEN1 J13. 18. Replace the left-side Diluter cover. 19. Perform Diluter Function F92, and adjust the low vacuum level, if needed. The reading should be 6.000 ±0.010.
Page 161 TROUBLESHOOTING FLUSHING THE BACKWASH TANK VACUUM SUPPLY LINE 20. Remove the right-side Diluter cover to access the Low Vacuum Regulator. 21. Use the adjustment wheel on the upper right-side corner to make the respective adjustments. The +/- sign is above the wheel.
Page 162 TROUBLESHOOTING FLUSHING THE BACKWASH TANK VACUUM SUPPLY LINE Remove the left-side Diluter cover by lifting up and out on the panel. Locate the VL107A, the white pinch valve located above the Backwash Tank. The top-fitting above VL107A is a feed-through fitting marked FF-5. Disconnect the I-beam from the FF-5.
Page 163 TROUBLESHOOTING FLUSHING THE BACKWASH TANK VACUUM SUPPLY LINE Fill the syringe with distilled water and attach the syringe tubing to FF-5. The water will fill the Vacuum Overflow Tank (VC22). (The VC22 is located towards the bottom of the left panel, behind the rocker bed.) Use enough water so that VC22 is half-full.
Page 164 TROUBLESHOOTING FLUSHING THE BACKWASH TANK VACUUM SUPPLY LINE Press the Reset button on the Analyzer. This will drain the Vacuum Overflow Tank (VC22). To confirm that the vacuum supply line is open, perform Diluter Function F30 to activate the Backwash Tank Check and verify that no error messages are generated.
Page 165: Error Messages,
ERROR MESSAGES SYSTEM AND WORKSTATION MESSAGES The LH 700 SERIES System automatically monitors many of its activities and conditions and generates messages to alert the operator when the system does not respond as expected. Messages that apply to the LH 700 SERIES System are described in the tables in Heading 6.4, ANALYTICAL STATION MESSAGES;...
Page 166: About, Workstation Messages,
If the Workstation is connected to a network, some messages may be caused by the network configuration. If a local network administrator or Windows NT administrator is available, contact the administrator for help resolving the problem prior to calling your Beckman Coulter Representative. PN 773023AC...
Page 167 ERROR MESSAGES SYSTEM AND WORKSTATION MESSAGES Icons and Traffic Lights Displayed on the Workstation with Workstation Only Messages The Patient icon is displayed on the Command Center when patient sample results are pending to be reviewed for validation in the review folder. The Host Communication icon is displayed on the Command Center when sample transmissions are pending to be transmitted and the LIS/HIS is not responding.
Page 168: Diluter Errors,
ERROR MESSAGES DILUTER ERRORS DILUTER ERRORS Any of the following messages may precede a DILUTER ERROR message. ASPIRATION N--- ASPIRATION -P-- ASPIRATION --B- ASPIRATION ---C BED NOT ADVANCED BED NOT BACKWARD BED NOT FORWARD BED NOT LEVEL BELLOWS NOT DOWN Bellows Not Down BELLOWS NOT UP Bellows Not Up...
Page 169: Non-Diluter Errors,
ERROR MESSAGES NON-DILUTER ERRORS NON-DILUTER ERRORS Any of the following messages may precede a NON-DILUTER ERROR message. AMC, Diluter, Or DIFF Processor Hardware Malfunction ANALYZER ERROR BACKWASH TANK NOT FULL BACKWASH TANK STILL FULL Diluent Level Low Or Faulty Backwash Tank Float Sensor Diluent Level Low Or Faulty Sheath Tank Float Sensor Numeric Keypad Failure DPR DILUTER PACKET ERROR...
Page 170 ERROR MESSAGES NON-DILUTER ERRORS PN 773023AC...
Page 171 NOT READY Instrument Log Data not transmitted. Stopped - inoperable. DIFF PROCESSOR card 1. Reset the Analytical Station. Communication Error communication problem. 2. If message persists, call your Beckman Coulter AMC-DIFF communication Representative. failure: 286 board. Reset Analytical Station. PN 773023AC...
Page 172 (depends upon when error AMC. 2. If message persists, reseat the AMC. Diluter not receiving data occurred). from AMC. Reset 3. If message still persists, call your Beckman Coulter Analytical Station. Representative. AMC Never Sent Start NOT READY AMC NEVER SENT Instrument Log Stopped - inoperable.
Page 173 Verify air filter is clean. occurred). unless the error condition c. Turn Power Supply MAIN POWER switch ON. is corrected. 3. If message still persists, call your Beckman Coulter Representative. Analyzer Error SYSTEM FAULT ANALYZER ERROR Instrument Log Data displayed if available Stopped - inoperable.
Page 174 Instrument Log Data displayed if available Stopped - inoperable. Communication error. 1. Reset the Analytical Station. (depends upon when error 2. If message persists, call your Beckman Coulter AMC reported error - occurred). ASCII Receive Error. Reset Representative. Analytical Station.
Page 175 TROUBLESHOOTING ANALYTICAL STATION MESSAGES Table 6.2 System Messages - A (Continued) Analytical Station Workstation Analytical Station Status/Recovery Analyzer Diluter Beep Log Message and Analyzer Alert Message Status Message Screen Message Icon on Command Center Sample Results Analytical Station Status Probable Cause Corrective Action Aspiration ---C (current status)
Page 176 Check previous messages (<Tube ID>, 3. Reset the Analytical Station. <Cass/Pos>). 4. If unable to correct the problem, or if the message recurs, call your Beckman Coulter Representative. You can run samples in the Manual mode. 6-12 6-12 PN 773023AC...
Page 177 3. Reset the Analytical Station. MANUAL MODE tension or cassette 4. Continue normal operation. If message persists, position (<Tube ID>, call your Beckman Coulter Representative. You can <Cass/Pos>). run samples in the Manual mode. Bed Not Backward SYSTEM FAULT BED NOT...
Page 178 3. Continue normal operation. If message persists, check for leaks, kinks or plugs around the needle. 4. If message still persists, replace the needle. 5. If message still persists, call your Beckman Coulter Representative. You can run samples in the Manual mode.
Page 179 3. Reset the Analytical Station. rerun. process, but it will prevent 4. If the message persists, call your Beckman Coulter initiation of a new sample, Representative. You can place the cassette on the unless the error condition rocker bed and press START/CONT to continue is corrected.
Page 180 3. Turn the Power Supply MAIN POWER switch ON. (depends upon when error temperature >80°C). initiation of a new sample, 4. If message persists, call your Beckman Coulter occurred). unless the error condition Representative. is corrected.
Page 181 2. Reset the Analytical Station. pressure or voltage error Keypad) until error is error detected at power up. detected. Reset Analytical 3. If message persists, call your Beckman Coulter resolved. Station. Representative. If this error occurs it will not halt the action in...
Page 182 2. Power up the Analytical Station: appear. occurred). unless the error condition a. Turn on the MAIN POWER switch. is corrected. b. Press POWER ON. 3. If the message persists, call your Beckman Coulter Representative. 6-18 6-18 PN 773023AC...
Page 183 Turn on the MAIN POWER switch. is corrected. b. Press POWER ON. 3. If the message persists, call your Beckman Coulter Representative. Diff RAM Test Failed Instrument Log Data not transmitted. Idle - ready for request.
Page 184 2. Power up the Analytical Station: occurred). unless the error condition a. Turn on the MAIN POWER switch. is corrected. b. Press POWER ON. 3. If the message persists, call your Beckman Coulter Representative. Dil SOL/OP PS 24V (current status) DIL SOL/OP PS Instrument Log Data not transmitted, or data Stopped - inoperable.
Page 185 2. Power up the Analytical Station: occurred). unless the error condition a. Turn on the MAIN POWER switch. is corrected. b. Press POWER ON. 3. If the message persists, call your Beckman Coulter Representative. Dil4 Card 12V Error (current status) DIL4 CARD Instrument Log...
Page 186 Data displayed if available Stopped - inoperable. Diluter echo not understood by 1. Reset the Analytical Station. (depends upon when error Analyzer. 2. If message persists, call your Beckman Coulter Diluter communication occurred). failure. Reset Analytical Representative. Station. Diluter Error...
Page 187 Stopped - inoperable. Power Supply valve 1. Reset the Analytical Station. Closed STUCK CLOSED malfunction. Overflow valve 2. If message persists, call your Beckman Coulter Representative. malfunction (remained closed) detected while turning compressor ON. Reset Analytical Station. PN 773023AC 6-23...
Page 188 Turn on the MAIN POWER switch. b. Press POWER ON. 3. If the message persists, call your Beckman Coulter Representative. as Fatal DPR Error SYSTEM FAULT Instrument Log Data displayed if available Stopped - inoperable.
Page 189 UNCLOG 3 function once. 6. Allow Analytical Station to sit idle for 30 minutes. Then perform F44 FLOWCELL UNCLOG 1 function before analyzing samples. 7. If message persists, call your Beckman Coulter Representative. Function Not Allowed (current status) Instrument Log...
Page 190 Corrective Action HGB Status Error (current status) HGB STATUS Instrument Log Data not transmitted, or data Paused until error Hgb lamp power supply Call your Beckman Coulter Representative. ERROR transmitted and “System resolved. defective Workstation Alarm” message printed in Messages:HGB lamp...
Page 191 2. Power up the Analytical Station: occurred). unless the error condition a. Turn on the MAIN POWER switch. is corrected. b. Press POWER ON. 3. If the message persists, call your Beckman Coulter Representative. Histogram Mem. Reset NOT READY Instrument Log Data not transmitted.
Page 192 Stopped - inoperable. AMC, Diluter/Solenoid/Optics 1. Reset the Analytical Station. INTERRUPT (depends upon when error Power Supply or DIFF AMC, Diluter or DIFF 2. If message persists, call your Beckman Coulter occurred). PROCESSOR cards’ hardware Representative. Processor hardware NOT READY malfunction.
Page 193 Check label and scanner. Rerun sample. 3. Continue operation. If message persists, use a different cassette. 4. If message still persists, call your Beckman Coulter Representative. Laser Voltage Lower (current status) Instrument Log Data not transmitted, or data If AutoStop/Flow Cell Voltage is 1181 to 1230 Vdc.
Page 194 (left lift not up) or faulty 3. Continue normal operation. If message persists, MANUAL MODE sensor (<Tube ID>, call your Beckman Coulter Representative. You can <Cass/Pos>). Remove run samples in the Manual mode. obstruction and restart Analytical Station. Left Load Fail...
Page 195 2. Press START/CONT to resume normal operation. not halt the action in process, but it will prevent 3. If the message recurs, call your Beckman Coulter initiation of a new sample, Representative. unless the error condition is corrected.
Page 196 6.1-6.2 in. Hg and/or low low vacuum reading cannot be adjusted, call your Low vacuum drifted. vacuum regulator and/or Beckman Coulter Representative). Adjust low vacuum. If low If this error occurs it will vacuum pump defective. 2. If in a Shutdown mode, press STOP.
Page 197 4. Continue normal operation and watch for sensor and rerun sample. messages. 5. If additional messages appear, follow the corrective actions associated with those messages. 6. If this message persists, call your Beckman Coulter Representative. Monitor Task Did Not SYSTEM FAULT Instrument Log Data not transmitted.
Page 198 Stopped - inoperable. Communication error. 1. Reset the Analytical Station. Data FOR AMC DATA (depends upon when error 2. If message persists, call your Beckman Coulter Diluter not ready to occurred). receive data from AMC. Representative. Reset Analytical Station. Operator Alert 09...
Page 199 Data not available. Stopped - inoperable. Workstation not receiving data 1. Reset the Analytical Station and/or Workstation. from Analytical Station. 2. If message persists, call your Beckman Coulter AMC Reported Error - PC Representative. not ready. Reset Analytical Station. Peltier Heater Sensor...
Page 200 TRUCK faulty 2. Reset the Analytical Station. Manual sampling probe NOT HOME wipe obstructed (not r Probe-wipe mechanism is 3. If message persists, call your Beckman Coulter NOT READY obstructed or faulty. home) (<Tube ID>, Representative. <Cass/Pos>). Remove obstruction. Probe Wipe Up Fail...
Page 201 SYSTEM FAULT Instrument Log Data not transmitted, or data Stopped - inoperable. -15 V power to power supply 1 Call your Beckman Coulter Representative. transmitted and “System outside -5% limit. Power Supply 1 problem: Alarm” message printed in -15 Volts below limit. Call...
Page 202 Beeps- operation Harness not connected. 1. Reset the Analytical Station. Connected CONNECTED continues. 2. If message persists, call your Beckman Coulter The probe wipe harness is not connected. Reset If this error occurs it will Representative. Analytical Station. not halt the action in...
Page 203 Instrument Log Data not transmitted. Stopped - inoperable. Red and white counter did not 1. Reset the Analytical Station. reset to zero value. 2. If message persists, call your Beckman Coulter Red/white processor Representative. counter(s) did not reinitialize. Reset Analytical Station.
Page 204 If AutoStop/Flow Cell Reading <5.8 V. 1. Reset the Analytical Station. transmitted and “System switch is OFF, run 2. If message persists, call your Beckman Coulter RF (6.3V) voltage reading Alarm” message printed in continues. below limit (<Tube ID>, Representative.
Page 205 R/W Data Mem. NOT READY Instrument Log Data not transmitted. Stopped - inoperable. Internal memory error. 1. Reset the Analytical Station. Interference Analyzer memory 2. If message persists, call your Beckman Coulter Representative. read/write failure. Reset Analytical Station. PN 773023AC 6-41...
Page 206 Check for rocker bed while the rocker bed obstruction. advances on the rocker bed. was still active. 3. If the message persists, call your Beckman Coulter Representative. You can run samples in the Manual mode. Sample Removed Too (current status)
Page 207 2. Power up the Analytical Station: unless the error condition a. Turn on the MAIN POWER switch. is corrected. b. Press POWER ON. 3. If the message persists, call your Beckman Coulter Representative. SPC Power Supply Bad (current status) Instrument Log Data displayed if available Stopped - inoperable.
Page 208 Instrument Log Data displayed if available Stopped - inoperable. Internal software problem. 1. Reset the Analytical Station. (depends upon when error 2. If message persists, call your Beckman Coulter Internal software failure. occurred). Reset Analytical Station. Representative. Stain Heater Disabled...
Page 209 (not out) or faulty sensor. 3. Continue normal operation. If the message recurs, MANUAL MODE Remove obstruction and call your Beckman Coulter Representative. You can restart Analytical Station. run samples in the Manual mode. STX Rec. Error NOT READY Instrument Log Data not transmitted.
Page 210 Stopped - inoperable. Two NAKs detected while 1. Reset the Analytical Station. (depends upon when error receiving data from Diluter. Analyzer-Diluter 2. If message persists, call your Beckman Coulter occurred). Representative. Communication failure: two NAKs sent from Diluter. Reset Analytical Station.
Page 211 Reset Analytical 3. If additional messages appear, follow the corrective appear. Station. actions associated with the messages. 4. If this message persists, call your Beckman Coulter Representative. Vls Air Error MANUAL MODE VLS AIR ERROR Instrument Log...
Page 212 Invalid command received by 1. Reset the Analytical Station. Interpretation XX RECEIVED (depends upon when error Diluter. 2. If message persists, call your Beckman Coulter Diluter received an invalid occurred). Representative. command from the AMC. Reset Analytical Station. Wrong Command...
Page 213: Workstation Messages,
2. If the problem persists, reset the Analyzer. 2. Communications error between Workstation and Analyzer. 3. If the problem persists after Analyzer reset, call your Beckman Coulter Representative. Auto numbering has been enabled. Starting auto number is...
Page 214 <flag> limit Test parameters are HGB blank, HGB read, WBC, RBC, HGB, 2. If the message persists, call your Beckman Coulter Representative. PLT, DIFF, RETIC, sheath pressure, DIFF pressure, DIFF LS offset, RETIC LS offset, Laser voltage, RF300 Volts, RF6.3 Volts, and ambient temperature.
Page 215 10,000 ±1,000 and repeat the carryover test. outside the predefined limits. Carryover parameters are 2. Otherwise, repeat the carryover test with the same specimen. WBC, RBC, HGB, PLT, DIFF and RETIC. 3. If the message persists, call your Beckman Coulter Representative. Carryover passed. Carryover Ready All parameters for the last carryover test performed on Status only;...
Page 216 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.18 Workstation Messages - C through G (Continued) Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Control lot <lot#> is Inactive, Sample will be stored as a Control An inactive Lot# matched the sample ID received from If the sample was intended to be stored as a control, change the inactive control...
Page 217 2. If the clock is not running, reset the Analytical Station. with the same date and time. 3. If the clock is still not running, call your Beckman Coulter Representative. Flow cell voltages/pressures out of specifications (<Run Instrument “System...
Page 218 Host/ToDo Ready Data transmitted by Host to Workstation (ToDo List 1. LIS should retransmit. upload) was unrecognizable or inconsistent. 2. If the message persists, call your Beckman Coulter Representative. Host: Bad TestName in TestField Host/ToDo Abort Ready for next The data received in Host Transmission is not in the See Host Transmission Specification for correct format.
Page 219 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.19 Workstation Messages - H (Continued) Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Host: ID1 With TestType Pending Duplicate <Sample ID>, Host/ToDo Abort Ready for next The data received in Host Transmission is not in the See Host Transmission Specification for correct format.
Page 220 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.19 Workstation Messages - H (Continued) Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Host: No SOH in preamble Host/ToDo Ready for next The data received in Host Transmission is not in the See Host Transmission Specification for correct format.
Page 221 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.19 Workstation Messages - H (Continued) Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Host: Number Of Fields Count is 0 Host/ToDo Abort Ready for next The data received in Host Transmission is not in the See Host Transmission Specification for correct format.
Page 222 Communications between the Workstation and the 1. Reset the Analytical Station Analytical Station malfunction Analytical Station were interrupted. 2. Shutdown and restart the Workstation. 3. Check cable connections. 4. If message persists, call your Beckman Coulter Representative. 6-58 6-58 PN 773023AC...
Page 223 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.21 Workstation Messages - L through M Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Last Two XB batches are outside limits. Analytical Station Last XB batch is outside limits. 1.
Page 224 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.21 Workstation Messages - L through M (Continued) Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Last Diff XM batch outside limits condition has cleared. Ready Last Diff XM batch was out of limits and current Diff XM Status only: no action necessary.
Page 225 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.21 Workstation Messages - L through M (Continued) Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Last two Diff XM batches are outside limits. Analytical Station The last XM batch is outside limits.
Page 226 Analytical Station 4. Clean the bar-code reader. r Label is smudged. stops processing 5. If the message persists, contact your Beckman Coulter Representative. r Label is misplaced. samples. r Bar-code reader is dirty. NO READ: Sample run received without proper identification...
Page 227 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.23 Workstation Messages - O Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Operator <Operator ID> added <Location Name> to the Event Operator added the specified location to the location Status only;...
Page 228 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.23 Workstation Messages - O (Continued) Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Operator <Operator ID> enabled Extended QC Rule Limits Control Ready Operator enabled the Extended QC Rule limits. Status only;...
Page 229 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.24 Workstation Messages - P through V Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Password changed. Event Operator currently logged on has changed his Status only;...
Page 230 TROUBLESHOOTING WORKSTATION MESSAGES Table 6.25 Workstation Messages - W through Z Workstation System Status/Recovery Applicable Sample Log Message and Icon on Command Center Results System Status Probable Cause Corrective Action Workload has been automatically reset Event Ready The workload recording is automatically reset (restart at Status only: no action necessary.
Page 231 INDEX detector, drip plate, 2-23 access, 5-26 sampling valve, 2-15, 5-23 cycle control, 5-26 BSV, 2-15, 2-23, 5-23 add, 5-39 clean outside, 2-15 laboratory procedures, 5-39 drip plate, 2-23 adjust, 4-2, release, 5-23 Hgb lamp, bubbles, 5-23 tube detector, burn, advance, 5-27 Advancing the Tube,...
Page 232 INDEX needle assembly, 3-10 F01, rocker bed, 2-32 F02, 5-18 VIC, 2-37 F03, 5-23 waste chamber, 2-39 F04, 5-13 zapping apertures, 2-14 F05, 5-4, cleaning agent prime, 5-18 F06, 5-23 clearing, 2-24, 5-15, 5-16, 5-17, 5-20, 5-25 F08, 5-21 dispense Retic clearing solution, 5-25 F09, 2-14...
Page 233 INDEX F22, 5-13 F23, 5-12 electrode, 2-26 F24, 5-12 electronic, 5-38, 5-39 F25, 5-24 enabling, 4-4, 5-26 F26, 5-25 error, 5-38, 5-39 F27, Erythrolyse II, 5-12, 5-19 F28, 5-22 extended, 5-15 F30, F31, 5-24 F32, 5-14 F33, 5-14 F key, F40, 2-22 F01,...
Page 234 INDEX keypad, 5-17 PAK Clear, 5-25 PAK LYSE, 5-12, 5-19 PAK PRESERVE, 5-12, 5-13, 5-19 PAK Stain, 5-24 label, picture, 2-32, 2-34, 5-34 cassette, pinch valve, 3-20, 5-34 laboratory, 5-39 replace, 3-20 lamp, plate, 2-23 laser diode scanner, 2-33, pneumatics, latex, 5-7, 5-9, 5-10 Positioning the Advanced Bar-Code Reader,...
Page 235 INDEX -25, 3-26, 3-27 calibration, air filter, 2-2, sheath, 5-25 cassette, shutdown, cassette spring clip, solenoid, 5-4, 5-5, 5-25, 5-29, 5-43, 5-49 check valve, spring clip, fuses, StabiLyse, 5-12, 5-13, 5-19 needle, stain, 5-21, 5-24 pinch valve, 3-20 sweep flow, 5-21 reagent, 3-24, 3-25...
Page 236 INDEX AMC Reported Error - Retic Transmit Failure, 6-39 wait enable/disable, 5-26 AMC Reported Error - Run Aborted, PC Not wand, 2-33, Ready, 6-41 waste, 2-39, 3-27 AMC Reported Errors -- Operator Alert 09, 6-34 clean chamber, 2-39 AMC Requested Stop, replace container, 3-27 AMC, Diluter or DIFF Processor hardware...
Page 237 INDEX Automatic Mode Disabled, 6-12 Control folder for lot number <lot #> deleted, 6-51 Automatic sampling mode disabled, 6-12 Control folder for lot number <lot #>edited, 6-51 Auto-Numbering has been disabled, 6-49 Control lot is Inactive, Sample will be stored as a Auto-Numbering has been enabled auto number is Patient Sample, 6-52...
Page 238 INDEX Dil1 Card -15V Error, 6-21 Fan Power Bad, 6-24 Dil1 Card 5V Error, 6-20 Fatal DPR Error, 6-24 DIL4 Card 12V Error, 6-21 Faulty backwash tank float sensor, 6-13 Diluent front and rear sensors detected carryover Faulty diluter memory, 6-16 (<Tube ID>, <Cass/Pos>), 6-11...
Page 239 Last XB batch outside limit conditions has failed, 6-24 cleared, 6-59 Instrument has not performed a shutdown in <24> Left Lift Not Up, 6-30 hours. Beckman Coulter service recommends Left Load Fail, 6-30 shutdown every 24 hours, 6-65 Left Stack Full, 6-30 Intermittent PS1 Supply Error,...
Page 240 INDEX Manual sampling probe wipe obstructed (not up) Operator <OPERATOR ID> changed Reporting (<Tube ID>, <Cass/Pos>), 6-36 Units setup, 6-63 Maximum # Of Repro runs Exceeded - Sample Not Operator <Operator ID> changed Shifts Stored, 6-61 definition, 6-63 Maximum number of cassettes (12) already loaded, Operator <Operator ID>...
Page 241 INDEX limit, 6-36 Replace reagent container, 6-31 Power Supply 1 problem: -15 Volts above Reservoir low on CBC lyse, 6-31 limit, 6-36 Reservoir low on cleaner, 6-30 Power Supply 1 problem: -15 Volts below Reservoir low on DIFF PAK, 6-31 limit, 6-37 Reservoir low on diluent,...
Page 242 INDEX Sheath sample pressure below limit ‹<Tube ID>, Wrong Command Interpretation, 6-48 <Cass/Pos>›, 6-42 Wrong Command Sequence, 6-48 Sheath Tank Not Full, 6-43 Wrong Echo Xmit, 6-48 Sheath Tank Still Full, 6-43 Wrong Repro Type, latex Sample will not be SPC A/D Status Error, 6-43 stored,...
Page 243 TRADEMARKS 5C Series Control, Beckman Coulter, Coulter, Coulter Clenz, Coulter Counter, Lin-C, Lyse S, S-Cal, and Z Series are trademarks of Beckman Coulter, Inc.; SC Series Control, Beckman Coulter, the BECKMAN COULTER logo, COULTER, Coulter Clenz, Coulter Counter, Lin-C, Lyse S, S-Cal, and Z Series are registered in the USPTO and SIPO.
Page 244 COULTER LH 780 SYSTEM HARD-COPY DOCUMENTATION Host Transmission Specifications for transmitting to a host computer. PN 4277303 Available in hard copy by request. Reference Use and Function • Installation • Operation Principles • Specifications/Characteristics • PN 773021 Precautions/Hazards • References • Glossary • Index Available in hard copy by request.

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