Wasatch Photonics X Series Operation Manual

Wasatch Photonics X Series Operation Manual

Raman spectrometer
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Wasatch Photonics
X series Raman Spectrometer
Operation Manual
Document version: 1.0.7
October 27, 2023
808 Aviation Parkway, Suite 1400, Morrisville, NC 27560 USA
wasatchphotonics.com
| +1 919.544.7785

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Summary of Contents for Wasatch Photonics X Series

  • Page 1 Wasatch Photonics X series Raman Spectrometer Operation Manual Document version: 1.0.7 October 27, 2023 808 Aviation Parkway, Suite 1400, Morrisville, NC 27560 USA wasatchphotonics.com | +1 919.544.7785...
  • Page 2: Table Of Contents

    2.6. Laser Firing Delay 2.7. Laser Interlock Key-Switch 2.8. Remote Laser Interlock 2.9. Laser Software Control Unboxing Your X series Raman Spectrometer Optical Coupling 4.1. ILP (Integrated Laser and Probe) 4.2. ILC (Integrated Laser and Interchangeable Coupling) 4.3. IC (Interchangeable Coupling) 4.4.
  • Page 3 IC (Interchangeable Coupling) iv. IP (Integrated Probe) External Block Diagrams ILP (Integrated Laser and Probe) ILC (Integrated Laser and interchangeable Coupling) iii. IC (Interchangeable Coupling) Appendix: Mechanical Drawings ILP (Integrated Laser and Probe) X series Operations Manual Oct 27, 2023...
  • Page 4 Continuous Wave PWM vs “Pulsed” Appendix: Electrical Connectors and Pin-outs 12VDC Power USB 46 Remote Laser Interlock Appendix: Remote Interlock Sample Configurations Configuration 1: Integrated Laser (Remote Interlock) Configuration Two: External Laser Tested Vendor Products X series Operations Manual Oct 27, 2023...
  • Page 5: Introduction

    Raman measurements of the world around you. 1.1. Applicability This manual is applicable to all Wasatch Photonics X series Raman spectrometers, including all laboratory and OEM variants of the following: ● WP 532X ● WP 638X ●...
  • Page 6: Laser Safety

    Page 5 Laser Safety Wasatch Photonics X series Raman spectrometers with integrated lasers include a number of safety features to minimize risk of exposure and injury to the operator and any bystanders. Nevertheless, be aware that these remain Class 3B laser products and are therefore fundamentally unsafe for use without appropriate Personal Protective Equipment (PPE), such as laser goggles, and user training from a qualified instructor and syllabus.
  • Page 7: Safety Markings

    This can protect the optics from dust and damage when not in use, and represents an additional safety feature by physically preventing inadvertent laser emission when emplaced. For convenience, a magnetized clip is provided to hold the attenuator when not engaged. X series Operations Manual Oct 27, 2023...
  • Page 8: Light-Tight Optical Bench

    2.4. Light-Tight Optical Bench Wasatch Photonics Raman spectrometers are designed such that coherent laser light cannot escape the optical bench and radiate into the environment, save through the intended optical coupling (freespace front optic for ILP models, and FC/PC fiber connector for ILC models).
  • Page 9: Laser Warning Leds

    2.5. Laser Warning LEDs Wasatch Photonics Raman spectrometers with integrated lasers have two warning LEDs on the front panel. These LEDs are designed to be visible both from the front and top of the spectrometer. When operational, the LEDs will flash in synchronization; if either LED is illuminated “solid” (not flashing), that indicates a fault condition and the unit should be referred to the manufacturer for investigation and repair.
  • Page 10: Laser Firing Delay

    2.8. Remote Laser Interlock Wasatch Photonics Raman spectrometers with integrated lasers include a remote laser interlock jack on the back face of the spectrometer (adjacent to the key-switch). This jack uses a standard stereo audio plug connector (SP-3540A, DigiKey 102-4779-ND), simplifying development of compatible plugs for your laboratory or OEM requirements.
  • Page 11: Laser Software Control

    For this purpose, Wasatch provides ENLIGHTEN™ as a cross-platform open-source desktop application, as well as a variety of software drivers and libraries which customers can use to design their own software. ENLIGHTEN™ is covered in section 8, and also on our website. X series Operations Manual Oct 27, 2023...
  • Page 12: Unboxing Your X Series Raman Spectrometer

    Page 11 Unboxing Your X series Raman Spectrometer Your Wasatch Photonics X series Raman spectrometer should come boxed with the following items: ● Spectrometer ● 12V power supply with mains cable for appropriate regional outlet ● USB cable (standard-A plug to standard-B plug) ●...
  • Page 13 Page 12 Cable tray in shipping box bottom For additional information on setting up your X series Raman spectrometer, please see the appropriate QuickStart guide found on the Technical Documents & Resources page on our website: https://wasatchphotonics.com/technical-resources/ X series Operations Manual...
  • Page 14: Optical Coupling

    Page 13 Optical Coupling Wasatch Photonics X series Raman spectrometers support four different optical coupling designs which allow different type of system setups: ● ILP (Spectrometer with Integrated Laser and Probe) for creation of a fully integrated system ● ILC (Spectrometer with Integrated Laser and Interchangeable Coupling) for creation of a semi- integrated system ●...
  • Page 15: Ic (Interchangeable Coupling)

    SMA-905 connector to collect Raman scatter from the sample. X series Raman spectrometer with ILC coupling Typically both fibers are joined in an external Raman probe like the Wasatch RP 785. It is important to use a probe built for the correct laser wavelength, as that determines the filter within the probe.
  • Page 16: Ip (Integrated Probe)

    Page 15 X series Raman spectrometer with IC coupling Besides having only the single SMA-905 fiber connector, these units are also visually distinguished by lacking any LEDs on the front face, as they have no integrated laser requiring warning signals. Likewise, the back face of these spectrometers lack other laser-safety interfaces such as the interlock key-switch or remote interlock socket.
  • Page 17: Measurement Setup

    However, Wasatch gratings and spectrometers are designed with low polarization dependence to ensure throughput remains consistent regardless of the input polarization state. Thus, as Raman scattering is inherently unpolarized, and given that it is a X series Operations Manual Oct 27, 2023...
  • Page 18: Sampling Accessories

    Note that the same probes can be used interchangeably between ƒ/1.3 and ƒ/1.8 ILC models, however, Wasatch Photonics RP series Raman probes are designed with an f/1.3 input aperture, and will thus match most efficiently with our f/1.3 spectrometer models.
  • Page 19: Vial Accessory (Ilp)

    5.3.1. ILP Models / Fully Integrated Systems An X series spectrometer with integrated laser and probe (ILP coupling) can be used as a fully integrated Raman system, typically with an additional cuvette holder/vial assembly, as shown below: To complete setup of the system, slide the cuvette holder/vial accessory over the freespace front optic/lens, then tighten down the thumb-screw on the vial accessory such that it can’t inadvertently be...
  • Page 20: Ilc Models / Semi-Integrated Systems

    5.3.2. ILC Models / Semi-integrated Systems An X series spectrometer with integrated laser (ILC coupling) allows creation of a semi-integrated Raman system, which can be completed by adding an external Raman probe and corresponding cuvette holder/vial assembly, as shown below: To complete setup of the system, attach the Raman probe to the spectrometer with fibers: 1) Connect the excitation fiber (keyed FC/PC connectors) to the spectrometer’s laser port on the...
  • Page 21: Ic Models / Fully Modular Systems

    5.3.3. IC Models / Fully Modular Systems An X series spectrometer (IC coupling) allows creation of a fully modular Raman system, which can be completed by adding a standalone laser, an external Raman probe, and the corresponding cuvette holder/vial assembly, as shown below: To complete setup of the system, attach the Raman probe to the laser and spectrometer with fibers: 3) Connect the excitation fiber (keyed FC/PC connectors) to the laser’s output port as per the...
  • Page 22 (nor ambient light intrude). Note that the position of the cuvette holder/vial accessory relative to the probe (i.e., the working distance) may need to be adjusted in and out to achieve maximum signal from the sample and minimum background fluorescence from the sample vessel. X series Operations Manual Oct 27, 2023...
  • Page 23: Power

    Page 22 Power All X series spectrometers are powered by mains through an included external AC/DC transformer. The transformer accepts 50-60Hz at 100-240VAC, and outputs 12VDC at 5A, providing up to 60W of power. Per-model operational power requirements including empirically measured current draw can be found ENG-0157 Empirical Power Draw and Power Supply Guidance.
  • Page 24: Communications

    Page 23 Communications All X series spectrometers communicate with their host controller through USB 2.0. They currently support USB 2.0 High-Speed communications (480 Mbps). 7.1. USB Communications The USB communications interface is detailed in our public engineering document ENG-0001 USB API, available on our website’s...
  • Page 25: Enlighten™ Quick Start

    By default, the Spectrum Chart displays free-running spectra; if you wish to pause this feature, simply click the Pause button above the chart, then resume with “Play”: X series Operations Manual Oct 27, 2023...
  • Page 26: Set Measurement Parameters

    Adjust integration time as needed to achieve your desired Raman signal intensity, using the on-screen arrows or pressing ctrl-T (Time). As you increase or decrease integration time, monitor the signal intensity with respect to the y-axis (signal counts) to achieve the desired signal level. X series Operations Manual Oct 27, 2023...
  • Page 27: Scan Averaging

    You can then save a spectrum by pressing ctrl-S or clicking the Save button above the spectrum chart. On Windows computers, measurements will be saved to your hard drive in the following location: X series Operations Manual Oct 27, 2023...
  • Page 28 Click “Export” to re-save all spectra from the “clipboard” at the left of the screen to a single wide, multi- measurement CSV file. This file can be found directly inside the “EnlightenSpectra” folder. X series Operations Manual Oct 27, 2023...
  • Page 29: Troubleshooting

    In this event, the spectrometer should be fully power-cycled (switched off, if it has a power switch, else unplugged for 10 seconds) and the software restarted. The spectrometer should then resume normal operations. X series Operations Manual Oct 27, 2023...
  • Page 30: Maintenance And Servicing

    RMA for factory service. 10.2. Replacement Power Transformer Wasatch Photonics X series spectrometers ship with a 12V “wall-wart” power brick (AC/DC transformer). If yours is damaged or lost you can obtain a replacement from Wasatch ( EDAC EA10683N-120).
  • Page 31: Appendix: Block Diagrams

    Page 30 A. Appendix: Block Diagrams a. Internal Block Diagrams ILP (Integrated Laser and Probe) ILC (Integrated Laser and interchangeable Coupling) X series Operations Manual Oct 27, 2023...
  • Page 32: Ic (Interchangeable Coupling)

    Page 31 iii. IC (Interchangeable Coupling) IP (Integrated Probe) X series Operations Manual Oct 27, 2023...
  • Page 33: External Block Diagrams

    Page 32 b. External Block Diagrams ILP (Integrated Laser and Probe) ILC (Integrated Laser and interchangeable Coupling) X series Operations Manual Oct 27, 2023...
  • Page 34: Ic (Interchangeable Coupling)

    Page 33 iii. IC (Interchangeable Coupling) X series Operations Manual Oct 27, 2023...
  • Page 35: Appendix: Mechanical Drawings

    Page 34 B. Appendix: Mechanical Drawings All dimensions are in millimeters (mm). a. ILP (Integrated Laser and Probe) X series Operations Manual Oct 27, 2023...
  • Page 36: Ilc (Integrated Laser And Interchangeable Coupling)

    Page 35 b. ILC (Integrated Laser and interchangeable Coupling) X series Operations Manual Oct 27, 2023...
  • Page 37: Ic (Interchangeable Coupling)

    Page 36 c. IC (Interchangeable Coupling) X series Operations Manual Oct 27, 2023...
  • Page 38: Ip (Interchangeable Probe)

    Page 37 d. IP (Interchangeable Probe) Model available in OEM configuration only. Dimensioned drawings and models available through your sales channel or distributor. X series Operations Manual Oct 27, 2023...
  • Page 39: Appendix: Housing Labels

    On models containing an integrated laser, this label is placed on the side of the spectrometer’s housing. It contains important safety and regulatory information about the spectrometer. Text of the label varies for different excitation wavelengths as shown below. 638nm (ILP and ILC) X series Operations Manual Oct 27, 2023...
  • Page 40: 785Nm (Ilp And Ilc)

    Page 39 785nm (ILP and ILC) iii. 830nm (ILP and ILC) X series Operations Manual Oct 27, 2023...
  • Page 41: 1064 (Ilp And Ilc)

    Page 40 1064 (ILP and ILC) X series Operations Manual Oct 27, 2023...
  • Page 42: Appendix: Mpe, Nohd And Nhz

    (referenced below as UC), which helps “bridge the gap” between MPE (as defined by IEC 60825-1) and NOHD / NHZ. The following tables contain inputs and computed results for X series Raman Spectrometers (638/785/830X) based on optical design specifications and vendor datasheets.
  • Page 43: Ilp Models

    Page 42 a. ILP Models The following table is applicable for Wasatch Photonics X series ILP spectrometers with integrated laser probes (laser radiation is being emitted from the spectrometer’s objective lens). Parameter (unit) 785X 830X 638X Reference Excitation λ (nm) 638 WP datasheets beam divergence (ɑ, full ∠, deg)
  • Page 44: Ilc Models (Fc/Pc Connector)

    Page 43 b. ILC Models (FC/PC Connector) The following table applies to X series ILC units in which no Raman probe has been connected (laser energy is being measured directly out of the FC/PC spectrometer port). Parameter (unit) 785X 830X...
  • Page 45: Ilc Models (Raman Probe)

    Page 44 c. ILC Models (Raman Probe) The following table applies to X series ILC units fiber-coupled to an external Raman probe, where laser radiation is being emitted through the external probe’s objective lens. Parameter (unit) 785X 830X 638X Reference Excitation λ...
  • Page 46: Continuous Wave Pwm Vs "Pulsed

    Continuous Wave PWM vs “Pulsed” When computing laser safety parameters, it is important to distinguish between Continuous Wave (CW) lasers and “pulsed” lasers. All Wasatch Photonics integrated lasers are Continuous Wave (CW) solid- state diode lasers. Pulsed lasers use an excitation medium which must be pulsed (pumped) at some rate to build up photons within a sealed resonance chamber, and then an optical switch (typically a Q-switch such as a Pockels cell) is used to briefly open the chamber and release the photons in a single high-energy pulse.
  • Page 47: Appendix: Electrical Connectors And Pin-Outs

    2m length or less (longer cables may allow additional noise to couple into the system). c. Remote Laser Interlock This jack uses a standard stereo audio plug connector (CUI Devices SP-3540A, DigiKey 102-4779-ND). X series Operations Manual Oct 27, 2023...
  • Page 48: Appendix: Remote Interlock Sample Configurations

    If your external laser has a continuity interlock connector, you can wire that directly to the Interlock Controller. Alternatively, you can use an interlocked power switch to control power to any external light source, as shown above. X series Operations Manual Oct 27, 2023...
  • Page 49: Tested Vendor Products

    Page 48 c. Tested Vendor Products LaserMet ICS-6-24 ELISe Interlock Controller LaserMet ICS-KP14 Entry Keypad X series Operations Manual Oct 27, 2023...
  • Page 50 Page 49 LaserMet IS-MDC-12 Door Interlock LaserMet Door Signs X series Operations Manual Oct 27, 2023...
  • Page 51 Page 50 LaserMet ICS-OR-PB Exit Override Digital Loggers IoT Power Relay X series Operations Manual Oct 27, 2023...

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