Summary of Contents for GE FineLINE 35 oligo
- Page 1 GE Healthcare FineLINE™ 35 oligo column Operating Instructions Original instructions...
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Page 3: Table Of Contents
Reference information ............35 Specifications ......................35 Chemical resistance ..................... 36 Service and training .................... 37 Ordering information ................... 38 Material abbreviations ..................40 Appendix A Health and Safety Declaration/Liability Form .. 41 FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... - Page 4 Table of Contents FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 5: Introduction
35 oligo column. It also describes procedures for packing, operation, maintenance and troubleshooting in a safe way. Prerequisites In order to operate FineLINE 35 oligo column safely and according to the intended purpose the following prerequisites must be met: •... -
Page 6: Important User Information
All users must read the Safety Instructions in Chapter 2of these Operating Instructions before installing, using or maintaining the column. Do not operate FineLINE 35 oligo column in any other way than described in the user documentation. If you do, you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment. -
Page 7: Regulatory Information
Software texts and commands are identified by bold italic text. A colon is used to separate menu levels (e.g. File:Open refers to the Open option in the File menu). 1.2 Regulatory information This section lists the directives and standards that are fulfilled by FineLINE 35 oligo column. Manufacturing information... - Page 8 • used in the same state as it was delivered from GE Healthcare, except for alterations described in the user documentation or explicitly authorized by GE Healthcare. Regulatory compliance of...
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Page 9: Column Description
Design The FineLINE 35 oligo column design incorporates a number of features. The FineLINE 35 oligo column has a simple design. It comprises a number of complete units, including a hydraulically or manually adjustable adapter and special nets in the end-piece and adapter, which are easily assembled together. - Page 10 Simriz. PFR91 and Teflon™ (PTFE). In the current FineLINE 35 oligo column the top end-piece and adapter lid are made of PEEK. Previous versions have these parts made of POM. PEEK is necessary only if ACN is to be used in the hydraulic chamber.
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Page 11: Safety Instructions
2.1 Safety precautions Introduction The safety precautions in this section are grouped into the following categories: • Explosive environment • Flammable liquids • Personal protection • Installation • System operation • Maintenance FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... - Page 12 Safety instructions Safety precautions Explosive environment WARNING FineLINE 35 oligo column is not intended for usage in explosive environment. Flammable liquids WARNING Explosive or flammable liquids. If potentially flammable or explosive liquids are used, consult local authorities regarding local rules and regulations before use.
- Page 13 Emergency Stop for that system shall be used in case of an emergency. Refer to the Operating Instructions for the used pump or system before using the column. CAUTION To avoid precipitation keep all reagents at or above 18° C. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
- Page 14 Safety instructions Safety precautions Maintenance CAUTION Use only components and spare parts delivered or authorized by GE Healthcare. This equipment is classified with material specifications which must be preserved. CAUTION Remove spillage immediately. CAUTION Make sure that the column has been cleaned and contains no residual harmful substances before maintenance.
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Page 15: Labels
Serial no: 1234567 Made in Sweden 2.3 Recycling procedures When taking FineLINE 35 oligo column out of service, the following must be followed: • The equipment shall be emptied, decontaminated, cleaned and sterilized. • All local regulations shall be followed with regard to scrapping of the equipment. - Page 16 Safety instructions Recycling procedures FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 17: Installation
Installation 3 3 Installation The FineLINE 35 oligo column is delivered assembled, with the adapter fitted in the column. Packing list Item Article no. FineLINE 35 oligo column 28-9461-41 User manual 28-9649-57 Allen key 4 mm 19-0379-01 Allen key 2.5 mm 19-4442-01 Union 5/16”... - Page 18 Installation FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 19: Operation
NOTICE No other solvents than those specified in the Operating Instructions should be used as they could possible damage the system. NOTICE The column material is not compatible with tetrahydrofurane, THF. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... -
Page 20: Packing The Column
Please read the complete chapter before using the column! FineLINE 35 oligo column is intended for use with ÄKTA™ oligopilot™ 100 and the instructions in this section assumes that this is the case. - Page 21 7. Note: Keep the OD 1/8” tubing as short as possible. Start UNICORN method instruction PFlow AB (10 bar flow). Continue for 1.5 to 2.0 CV. Stop the flow. Start the run. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 22: Unpacking The Column
Disconnect the top end piece by turning the bayonet anti clockwise and lift it up Turn the column upside down and collect the support in a suitable vessel. Start the deprotection as described in the ÄKTA oligopilot User Manual. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... -
Page 23: Recommended Operating Conditions
That shortens the cycle times and reduces the risk of dilution of the synthesis reagents entering the column. Flowrate conversions Table 4-2. Unit conversion of flowrate in cm/hour into ml/min for FineLINE 35 oligo column. Velocity [cm/hour] Flow rate [ml/min] 9.62... -
Page 24: Scaling Up
Linear flow rates and the number of column volumes used during method development should generally be the same as those to be used during the envisaged full scale process. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... -
Page 25: Maintenance
Make sure that the column has been cleaned and contains no residual harmful substances before maintenance, remove spillage immediately. The FineLINE 35 oligo column is designed with a minimum of components, which facilitates cleaning and reliable operation. In normal routine operation, the column requires only little regular maintenance.l... -
Page 26: User Maintenance Schedule
Section 7.2 Chemical resistance‚ on page 36. 5.3 Storage If the FineLINE 35 oligo column will not be in use for an extended period in time, it is recommended to disassemble the column and then clean the parts according to Section 5.2 Cleaning‚... -
Page 27: Disassembly And Assembly
Maintenance 5 Disassembly and assembly 5.4 5.4 Disassembly and assembly Disassembly and assembly of the FineLINE 35 oligo column is very straightforward and therefore only basic instructions are necessary. WARNING Hazardous substances. When using hazardous chemical and biological agents, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the substances used. - Page 28 (c). If the adapter sticks to the column, use the wrench as shown in the illustration (d). Pull out the entire adapter from the column glass tube (e). Use the wrench supplied to remove the bottom filter unit (f) from the column. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
- Page 29 “1” on the column top end-piece (c). To lock the adapter, turn the adapter lid clockwise so that the slot is positioned directly above “2” on the top end-piece (d). FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 30: Component Maintenance
It is recommended to clean the filters and distributors at regular intervals (see Section 5.1 User maintenance schedule‚ on page 26) The FineLINE 35 oligo column is designed with a minimum of components, which facilitates cleaning and reliable operation. In normal routine operation, the column requires only a little regular maintenance. - Page 31 Tighten the bottom filter. Adapter filter unit replacement Step Action Unscrew the two screws that hold the adapter filter unit and remove it from the adapter rod. Gently pull out the filter unit. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
- Page 32 .Pull out the tubing from the adapter rod. Unscrew the adapter knob from the tubing using the plastic wrench supplied. Insert a new tubing, OD 1/8’’, 235 mm. Reassemble in reverse order. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 33: Troubleshooting
Solvents used not compatible with O-rings and/or gaskets. Refer to Section 7.2 Chemical resistance‚ on page 36. Connections to and from the pump not correct (loose or misplaced). Column not connected correctly. O-rings not positioned correctly. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... - Page 34 Troubleshooting FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 35: Reference Information
1 to 10 cm Design pressure 20 bar Flow relocity range 0 to 200 ml/min WARNING Design pressure MUST NOT BE EXCEEDED Note:Using Primer Support 200 according to recommended packing procedures adds no packing pressure. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... -
Page 36: Chemical Resistance
Ethanol 9003-99-0 100% Hydrochloric acid 9004-54-0 0.1 M Methanol 67-56-1 100% n-Propanol 71-23-8 100% Phosphoric acid 9066-91-5 Sodium hydroxide 95077-05-7 76-05-1 0.4% NOTICE The column material is not compatible with tetrahydrofurane, THF. FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... -
Page 37: Service And Training
Reference information 7 Service and training 7.3 Service and training Please contact your GE Healthcare representative regarding service and training for FineLINE 35 oligo column FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... -
Page 38: Ordering Information
2 1 2 2 Figure 7-1. FineLINE 35 oligo column exploded view, refer to Table 7-2 for explanations. Note: The locking device 56-3100-31 is not shown in the illustration. It fits between the Adapter Lid (3) and the Adapter tube (6). - Page 39 19-2145-01 Union 5/16” female / M6 Male 18-1127-76 T-connector, M6 female 19-2144-01 Allen key 2.5 mm 19-4442-01 Allen key 4.0 mm 19-0379-01 Wrench FineLine 351 18-1110-88 Wrench M6 19-7481-01 Operating Instructions 28-9649-57 FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
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Page 40: Material Abbreviations
Reference information Material abbreviations 7.5 Material abbreviations Abbreviation Meaning EPDM Ethylene propylene diene monomer ETFE ethylene-tetrafluoroethylene Perfluor rubber PEEK Polyetheretherketone Acetal plastic Tetrahydrofurane FFKM/FFPM Perfluoroelastomers FineLINE 35 oligo column Operating Instructions 28-9649-57 AA... -
Page 41: Appendix A Health And Safety Declaration/Liability Form
Failure to complete the form completely accurately and truthfully can endanger GE Healthcare Bio-Sciences personnel and may lead to a delay in servicing the equipment. Equipment not sufficiently cleaned prior to return to GE Healthcare Bio-Sciences may be subject to additional charges. - Page 42 Appendix A Health and Safety Declaration/Liability Form FineLINE 35 oligo column Operating Instructions 28-9649-57 AA...
- Page 44 Sweden All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information.