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Emergency Information:
1. Medical Emergencies: Contact 911 and McGill Security 514.398.3000
2. Leave TEM as is. Do NOT shut down the vacuum system.
3. If possible, turn off High Tension and Close Column Valve.
4. Exit the Room/Building.
Emergency Contact Information:
• David Mui – Staff Scientist: Cell 438.938.6886; Email: xuedongliu88@hotmail.com
• S. Kelly Sears – Facility Manager: Cell 514.576.1926; Email: kelly.sears@mcgill.ca
• Joaquin Ortega – Director: Office 514.398.6348; joaquin.ortega@mcgill.ca
(Prepared by David Liu, S Kelly Sears and Joaquin Ortega)
Safety
• Do not wander behind the microscope or step on cables.
• There is one computer for the CM200 TEM.
1. The support PC (SPC) is for taking digital images with the AMT XR41B CCD camera and
collecting spectra with the EDAX Genesis Energy Dispersive Spectrometer (EDS) and
Genesis software. Note: Ensure you copy your data at the end of each TEM session.
Data older than three months may be deleted without notice.
Protective Equipment
• Nitrile gloves for handling sample holder and safety glasses for filling liquid nitrogen
dewar.
1. Initial Set-up
• When the CM200 is operational, the White Standby and
Red Microscope Off buttons will be illuminated and the
White On button will be dark on the front console. Lit
buttons indicate their availability as emergency
functions while the microscope is running.
Philips CM200 200kV TEM
Simplified Operating Manual
2017.07.07
1
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Summary of Contents for Philips CM200
- Page 1 • Nitrile gloves for handling sample holder and safety glasses for filling liquid nitrogen dewar. 1. Initial Set-up • When the CM200 is operational, the White Standby and Red Microscope Off buttons will be illuminated and the White On button will be dark on the front console. Lit buttons indicate their availability as emergency functions while the microscope is running.
- Page 2 REPLACE the styrofoam cap on the top of the dewar. • The CRT screen will display the CM200 status startup page or, more likely, one of the Modes or Mode Selection pages. If the Ready light (on the pushbutton...
- Page 3 2017.07.07 • PRESS the Vacuum key on the CRT Screen to check the vacuum status of the instrument. It should read Ready at the top center of the CRT screen. VERIFY the Ion Getter Pump (IGP) value is lower than 20 Log. •...
- Page 4 2017.07.07 a. RESET the sample stage. b. Always keep light pressure on the purple goniometer surface when removing the sample holder. PULL the holder straight back without rotating until it stops moving. c. ROTATE the holder clockwise until it stops. This rotation moves the guide pin approximately from the 12 o’clock to 5 o’clock position.
- Page 5 2017.07.07 d. TRANSFER the TEM grid into the recess at the end of the holder using the tweezers. e. Use the pin tool to gently LOWER the clamp straight down to hold the grid securely. RETURN the tool to the base of the holder stand.
- Page 6 2017.07.07 e. After the pumping countdown reaches zero and the Red LED goes out, SUPPORT the purple goniometer surface with one hand and GRIP the holder securely with the other. Slowly ROTATE holder counterclockwise from 5 o’clock to 12 o’clock position. f.
- Page 7 2017.07.07 b. Also on the first Parameters page is the Emission setting. Increase the filament current to the saturation value by TURNING the Filament knob on the control panel which should ordinarily be left at 1. A higher value will produce a brighter beam image but will also shorten lanthanum...
- Page 8 2017.07.07 c. On the PC screen, OPEN the CCD camera program by CLICKING the AMTV600 icon. d. On the CRT, PRESS Ready, Mode, choose configuration page. Turn the filament knob slowly until the Ext voltage reads 3.8. As a rule of thumb, rotate the knob until you hear two clicks;...
- Page 9 2017.07.07 5. Focusing the Image and Diffraction Pattern a. Focusing the image with the image wobbler i. Set the Focus Step size (inner Step Size knob) to 5 and PRESS the D button to place the microscope in Diffraction mode. ii.
- Page 10 2017.07.07 c. LOCATE a region of interest on the sample using stage joystick. d. To accurately focus, PRESS Focus. image will magnify four times and enable the imaging of the carbon grain and fine tune your focus. Go to higher magnification.
- Page 11 2017.07.07 d. From the TEM Brightfield page, PRESS CompuStage; the CompuStage Register Control page will appear. e. PRESS the Alpha Wobbler key and the stage will automatically rock through a tilt range of +/- 15°. f. USE the Z control lever on the Joystick to move the specimen up or down to MINIMIZE the apparent movement.
- Page 12 2017.07.07 saturation limit of the filament. With the limit set (highlighted), the operator can turn the Filament knob clockwise indefinitely without fear of oversaturating the filament. Turn the filament up to a number that is 4 or 5 less than the indicated filament limit. c.
- Page 13 2017.07.07 interpupillary distance so that you can see through them with both eyes; then adjust each eyepiece so that the rough edges of the beam stop are in focus for each eye. When you are done, retract the beam stop and lift the small screen back out of view. 9.
- Page 14 2017.07.07 b. PRESS the Align button on the front console to access the Alignment Selection page. On the upper right- hand side of the page, press Rot Center that becomes highlighted. either voltage or current centering performed; it is not necessary to do both.
- Page 15 (The Aperture Memo lists the diameters and positions of the apertures currently installed in the CM200. It may be accessed from the TEM BRIGHTFIELD page by PRESSING the Modes key and then Configuration.)
- Page 16 2017.07.07 f. UNDERFOCUS the image with the Focus knob (turn counter clockwise from the crossover) to obtain a few Thon rings. g. PRESS the Stg button to OPEN the Stigmator Control page. If it is not already highlighted, PRESS the Obj key on this page. h.
- Page 17 2017.07.07 and forth through focus, from underfocus to overfocus) and observe if a "streaking" pattern emerges and changes direction between under- and overfocus. If the astigmatism has been corrected, the specimen will vary only in focus, with no streaking pattern evident.
- Page 18 2017.07.07 j. For a fixed collection time, SELECT the Preset dropdown menu on the right-hand side of the Toolbar. Select a spectrum collection time in seconds either from the presets or by typing in a value. k. In the adjacent dropdown menu, SELECT a Time Option: Clock Time, Live Time or ROI Counts.
- Page 19 2017.07.07 select an element to display the spectrum peaks on top of the collected spectrum. CLICK Add to add the element to the Elements list. g. To remove an element from the Elements list, HIHLIGHT it and CLICK Delete. h. The Z+ and Z- buttons can be used to scroll through the elements. i.
- Page 20 2017.07.07 REMOVE the sample holder. RETRIEVE your TEM grid. REINSERT the holder into the CompuStage/column of the microscope. CHECK the Scheduler to determine if there is another user after you. If you are NOT the last user of the day, refill the LN dewar.
- Page 21 2017.07.07 Notes: Camera Constant Lλ for SAED For L=240 mm, Lλ=73.6 mm Å For L=340 mm, Lλ=104.1 mm Å For L=470 mm, Lλ=146.4 mm Å For L=700 mm, Lλ=204.4 mm Å Camera Constant Lλ for NBD ...