Hitachi TM3000 Instruction Manual page 121

NOTE : Verify that the main unit is connected to the PC with a USB cable. The application
will not start if the USB cable is not connected. Also, the application will not start if
the Power switch on the main unit is turned off.
4.2.2 Fixing a Specimen and Adjusting its Height
1. Precautions to be Observed Before Loading a Specimen When setting a specimen,
use caution on the following items:
1a. Specimens that can Blow Up When Placed in a Vacuum An object with a gas or
liquid sealed in an outer skin, such as a balloon, can blow up when placed in a vacuum and
damage internal components. These specimens can be observed by breaking the outer skin
in advance.
Examples: Balloons (inflated and sealed), eggs (of any size or type), cherry tomatoes, citrus
fruits, and living things such as insects and fish
1b. Liquids and Liquid-State Materials If a solution is placed in a vacuum, after the
water has evaporated, the remaining substance can splash in the specimen chamber,
potentially contaminating and damaging internal components. These specimens can be
observed by removing water in advance.
Examples: Milk and pen ink
1c. Insulators When an insulator specimen is observed in the normal mode, the
specimen can build up charge, resulting in image deviation and brightness/contrast change.
In such a case, perform observation by switching to the charge-up reduction mode (see 4.2.6
2. and 4.3.6 5.).
1d. Magnetized Samples Magnetized samples not only can stick to the objective lens
and damage internal components; they can also adversely affect the imaging. Such samples
can be observed by completely demagnetizing them.
Examples: Magnets and magnetic recording media
1e. Miscellaneous Human bodies, substances harmful to the environment, materials that
cannot be immobilized and living things should not be observed.
NOTE : Because the inside of the specimen chamber is evacuated, some specimens,
when observed, can damage the system. Either avoid observing such specimens,
or observe them after they are pre-treated appropriately.
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