GE Amersham ImageQuant 800 User Manual page 47

Part
4
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6
7
8
9
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11
Fluorescence multiplex
In Chemiluminescence imaging, it is possible to perform sequential chemiluminescence
and fluorescence imaging without moving the sample. Up to two fluorescence dyes can
be imaged in addition to the chemiluminescence imaging. This makes further downstream
processing of the images easier. The option to add multiplex imaging is found in the
Advanced settings panel when creating a method.
To use the fluorescence multiplex imaging, use the following steps.
Step
1
2
Amersham ImageQuant 800 User Manual 29383813 AA
3 Handle methods in ImageQuant 800 control software
Function
Manual exposure
Automatic exposure
Open saved methods
Return to Home screen
Chemiluminescence tab
Colorimetric tab
Fluorescence tab
Toggle colorimetric marker
Action
Next to Fluorescence multiplex, tap Select.
Tap Select dye.
3.4 Chemiluminescence image capture
3.4.1 Chemiluminescence imaging
Part Function
15 Settings tab
16 Help
17 Show or hide advanced settings
18 Select binning
19 Select fluorescence multiplex
20 Change capture area
21 Start pre-capture
22 Start capture
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