Plate Layout Wizard Basics; Create Single Zone Titration1Layouts - HP D300 Operating Manual

5-9 Plate Layout Wizard basics

The Plate Layout Wizard may be used for several purposes, both simple and complex. Complex examples
―Plate Layout Wizard advanced
are described in
titration (a set of different concentrations or volumes) for many or all of a set of fluids. For example if you
would like each row of a 384 plate to be an 8 point triplicate, and use those concentrations for all 16 rows of
the plate for 16 different compounds, the Wizard can create this configuration much more quickly than a
series of copy and paste special commands. This simple case is described here in section 5-9-1, it is a
―single zone titration 1‖ because all the concentrations in the titration are evenly spaced in linear or log scale
(known as a ―single zone‖) and because each well only contains a single compound (known as a ―titration 2‖).

5-9-1 Create single zone titration1layouts

Ensure that the desired well plate type, either 96 or 384, is selected using the Well Plate drop down
menu prior to starting the Plate Layout Wizard.
1. In the HP D300 Digital Dispenser main operating window, click Edit and then Plate Layout
Wizard. The Plate Layout Wizard: Initialization dialog box appears. See
The Plate Layout Wizard can also be accessed by right-clicking on any well in the well plate
image and then selecting the Wizard option in the Well Plate menu.
Figure 5-16
2. If there were existing entries in the Fluids List or fluids in any wells in the well plate, the
following statement appears in the dialog box:
―Some fluid and/or well data already exists. How would you like to proceed?‖
Select one of the three options:
a.
○
To use the wizard to add fluid to empty wells remaining in the current plate configuration
only, select Leave all existing data alone.
○
To retain the fluids from the Fluids List and design a new plate configuration, select
Clear the well plate only; leave the fluids alone.
functions‖. In the simple case, you can replicate a single
Click Edit and then Plate Layout Wizard
HP D300 Digital Dispenser
Figure 5-16.
Select desired
well plate type
here first
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