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HV
The system can operate from 0-120 kV. The higher the V, the "harder" the electrons
will hit your sample. For biological samples, try using kV between 60-100 kV.
Filament V
The voltage is set by the technician when the filament is saturated (a process involved
in the filament mounting).
Beam µA
This beam current reflects the "number" of electrons hitting the sample.
Condenser
0= it is out, 1= large, 5= smallest. We generally use size 2 for Lab6 filament, size 1 for
aperture
Tungsten filament.
Objective
0= it is out. Aperture 1 will give the largest contrast image. We are leaving it on size 2.
aperture
Spot size
(1= smallest, 5= largest) The smaller the spot, the more light you get. Generally spot
size 3 is ok to use.
Magnification
A small magnetic lens comes into play and the beam shifts a bit. In HC field it happens
crossover
between 30-40K, while in HR between 50-60K
Beam µA
The is the "amount" of electrons. 8 µA is default. Lower it for critical samples.
Changing µA will affect the beam alignment.
Lab6
Lanthanum Hexaboride Filament. It's lifetime is about 1000 hours.
Screen camera
This is the camera which looks down on the fluorescent screen. As the camera chip is
not hit directly by the beam, this camera can take a lot of light exposure. It is a 14 bit
camera with 1024 x 1024 pixels.
Xarosa camera
This is the main camera and is mounted on the top-bottom. It is a 16 bit camera with
5120 x 4005 pixels.
HC field
High contrast mode. There is a mini lens which improved the contrast (will reduce a bit
the light). You can magnify up to 200K. This mode is generally used for most specimen.
HR field
This is used for higher magnification and can reach 600K. When switching over to HR,
notice that the beam gets brighter.
Lens reset
Pressing this button resets everything to morning setup. Use this if you "get lost".
TMP
Tubular Molecular Pump, a new and fast pumping system which will quickly remove
contamination from the column.
OS
Object stigma. It can be adjusted when you notice that your beam is not circular any
more.
Eucentric
You can focus the sample inside the goniometer instead of using the beam focus
mode. Adjusting eucentric height is crucial before tomography. It's done at 3-5K.
AMF
Automatic multiple fields. With Xarosa you can do 4x4 images. If you need larger
fields, you need to use "image navigation". Images will always be acquired with
"maximize" capture parameter.
Resolution
This system can give you 0,2 nm resolution at 100kV.
Common nomenclature explained
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