Hitachi PRIMAIDE 1440 Instruction Manual page 38

Absorption spectra
Component A
(a) Absorptiometry
Fig. 2-6 Spectroscopic Measurement of a Sample
In contrast, if the fluorescence spectra do not overlap (Fig.
3-6 (b)), quantitative analysis of each compound can be made by
selecting the appropriate fluorescence emission wavelength
(even if the absorption wavelengths of two components are the
same).
Excitation spectra
Component B
Containing Two Components
Table 3-1 compares the analytical information obtained via
absorptiometry and fluorometry.
Table 2-4 Comparison of Analytical Information
Attainable with Absorptiometry and Fluorometry
Absorptiometry
Absorption spectra only
(corresponding to excitation spectra)
2 - 10
Component A
(b) Fluorometry
•
•
Fluorescence spectra
Component B
Fluorometry
Excitation spectra
Fluorescence spectra