Materials Required But Not Provided; Storage; Features; Precautions For Use - NEC express5800 A2010b User Manual
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- User manual (574 pages) ,
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PrimeScript™ RT Master Mix （Perfect Real Time）
III. Materials Required but not Provided
- Thermal cycler
(or 37℃ water bath and 85℃ heat block)
- 0.2 ml and 1.5 ml microtubes (for reverse-transcription reaction)
- Micropipettes and tips (autoclaved)
IV. Storage
-20℃
V.
Features
(1) The reaction can be started simply by adding template RNA and water.
(2) Template cDNA for real-time PCR can be efficiently synthesized in a short time.
This kit is best suited for 2 step real-time RT-PCR.
(3) Optimal template cDNA for real-time PCR can be synthesized with 2 kinds of
reverse-transcription primers: Oligo dT Primer and Random 6 mer.
(4) A standard curve must be generated for the quantification of real-time RT-PCR.
To generate a precise standard curve, it is important to dilute the total RNA and
reverse-transcribed cDNA precisely to low concentrations. However, dilution
of templates with water or TE buffer may narrow the range of the curve due to
template instability at low concentrations. Using EASY Dilution (for Real Time PCR)
makes for more accurate results at low concentrations, facilitating the creation of a
wide-range standard curve.
VI. Precautions for Use
Read these precautions before use and follow them when using this product.
(1) Briefly centrifuge the 5X PrimeScript RT Master Mix before use and allow the
reagent to settle to the bottom of the tube. Since the 5X PrimeScript RT Master Mix
has high viscosity, you will need to perform pipetting slowly and carefully, and also
mix the reaction solution well.
(2) When dividing up the reagent, please be sure to use new disposable tips to avoid
contamination among samples.
(3) This product is a completely pre-mixed reagent with reverse-transcription primers
already added (Oligo dT Primer and Random 6 mers).
Note: This product cannot be used for reverse-transcription reactions using Gene-
4
Specific Primer.
Cat. #RR036Q
v201204Da_2
URL:http://www.takara-bio.com
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